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拉氏环田螺——中国现生田螺科一新记录属种

热带农业工程 2024年第2期48卷 159-161页

作者：宁鹏飞丁煌英李明会谷德贤天津市水产研究所天津300221 天津市城市规划设计研究总院有限公司天津300190 西南林业大学林学院云南昆明650224

2013年4月8日于中国云南省西双版纳州勐腊县境内澜沧江一级支流南腊河采获田螺1种,经鉴定为拉氏环田螺Anulotaia lagrandierei (Bavay,1898)。该属该种为国内首次报道,并做描述。

关键词：新记录环田螺属分布

Notch1通路调控CD4^(+)CD25^(+)Foxp3^(+)调节性T细胞在肾血管性高血压中的作用

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中华高血压杂志（中英文） 2024年第4期32卷 363-370页

作者：余德宪郑娜尹丽娟陈旸彭琳何发静陈文娟四川省医学科学院·四川省人民医院肾内科四川成都610072 四川省医学科学院·四川省人民医院心内科四川成都610072

目的探索Notch1通路调控CD4^(+)CD25^(+)Foxp3^(+)调节性T细胞(Treg)在肾血管性高血压(RVH)中的作用与机制。方法选择2019年1月至2022年1月在四川省人民医院肾内科诊治为RVH的患者220例(RVH组),以及同期普通非RVH原发性高血压患者220... 详细信息

目的探索Notch1通路调控CD4^(+)CD25^(+)Foxp3^(+)调节性T细胞(Treg)在肾血管性高血压(RVH)中的作用与机制。方法选择2019年1月至2022年1月在四川省人民医院肾内科诊治为RVH的患者220例(RVH组),以及同期普通非RVH原发性高血压患者220例(原发性高血压组),另选择健康体检者220人为对照组,经倾向性评分匹配后,每组纳入10例。采用流式细胞术测定外周血单个核细胞(PBMC)中CD4^(+)CD25^(+)Foxp3^(+) Treg比例、白细胞介素-10(IL-10)、转化生长因子β(TGF-β)和细胞增殖抗原Ki-67水平;采用实时荧光定量PCR测定Notch1、Notch配体δ样配体(DDL)1、DDL4、Jagged1、Jagged4的mRNA水平;采用Western-blot法测定Notch1、hes家族bHLH转录因子(Hes)1、Hes5蛋白水平。结果与对照组比较,RVH组PBMC中CD4^(+)CD25^(+)Foxp3^(+)Treg比例降低,TGF-β、Ki-67表达水平明显降低,IL-10表达水平升高(均P<0.001);与RVH组比较,原发性高血压组PBMC中CD4^(+)CD25^(+)Foxp3^(+)Treg比例升高,TGF-β和Ki-67表达水平升高,IL-10表达水平降低(均P<0.001)。实时定量PCR结果显示,RVH组患者PBMC中Notch受体Notch1 mRNA、DLL1、DLL4、Jagged1和Jagged2 mRNA相对表达量也高于对照组和原发性高血压组(P<0.001)。Western-blot结果显示,患者外周CD4^(+)CD25^(+)Foxp3^(+)Treg加入Notch1信号通路抑制剂后,Hes1与Hes5表达下降,CD4^(+)CD25^(+)Foxp3^(+)Treg占比和TGF-β表达水平上升(均P<0.001)。结论 Notch1信号通路在CD4^(+)CD25^(+)Foxp3^(+)Treg调节的RVH中起着免疫调控作用,调节Notch1信号通路可明显促进CD4^(+)CD25^(+)Foxp3^(+)Treg的增殖和分泌抑炎因子的能力。

关键词：肾血管性高血压 CD4^(+)CD25^(+)Foxp3^(+)调节性T细胞 Notch1通路转化生长因子-β

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长江上游重庆段近20 a降水时空变化特征

地球环境学报 2024年第2期15卷 342-356页

作者：曾春芬陈钰婕杨奇方德贤三峡库区地表过程与环境遥感重庆市重点实验室重庆401331 重庆师范大学地理与旅游学院重庆401331 渝北区气象局重庆401120 重庆市人工影响天气办公室重庆401147

全球气候变暖背景下,我国降水分布日趋复杂,气象灾害造成的损失巨大。为满足防灾减灾与水资源管理的数据支撑需求,基于长江上游重庆段近20 a降水数据,利用线性回归、M-K非参数检验等方法,探究降水的时间变化趋势与空间分异特征。结果表... 详细信息

全球气候变暖背景下,我国降水分布日趋复杂,气象灾害造成的损失巨大。为满足防灾减灾与水资源管理的数据支撑需求,基于长江上游重庆段近20 a降水数据,利用线性回归、M-K非参数检验等方法,探究降水的时间变化趋势与空间分异特征。结果表明:近20 a重庆市年降水量在875.6—1348.2 mm。时间上,春、秋两季呈明显增多的趋势,其中3月最显著。降水在14 a时间尺度的周期过程线中波动最强,是第一主周期。空间分布上,呈西低东高的特点,在渝西和长江河谷地区降水相对偏少,主要受重庆地形的影响;渝西和渝东北地区秋季降水显著增多。研究结果可为长江流域旱涝灾害防治、水资源开发利用及经济社会可持续发展提供数据支撑,同时可为植被修复与生态廊道保障提供参考。

关键词：长江上游降水时空变化地形复杂区重庆市

氟苯尼考影响子宫内膜基质细胞蜕膜化的功能研究

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重庆医科大学学报 2024年第7期49卷 838-843页

作者：何佳仪代德贤李伟轲高茹菲王应雄龙菁重庆医科大学公共卫生学院教育部生殖与发育国际合作联合实验室重庆400016

目的:探究氟苯尼考(florfenicol,FLO)暴露对蜕膜化的影响及线粒体在其中发挥的作用。方法:构建FLO暴露的原代小鼠蜕膜基质细胞(mouse decidual stromal cells,mDSCs)模型,经FLO(1.56、6.25、25.00μg/mL)暴露后,采用鬼笔环肽染色法观察... 详细信息

目的:探究氟苯尼考(florfenicol,FLO)暴露对蜕膜化的影响及线粒体在其中发挥的作用。方法:构建FLO暴露的原代小鼠蜕膜基质细胞(mouse decidual stromal cells,mDSCs)模型,经FLO(1.56、6.25、25.00μg/mL)暴露后,采用鬼笔环肽染色法观察细胞形态变化,采用Western blot检测蜕膜化标志分子HOXA10表达以分析FLO对蜕膜化的影响;采用流式细胞术检测细胞凋亡,采用Western blot检测细胞增殖标志分子PCNA表达以分析FLO对蜕膜化过程中细胞增殖、凋亡的影响;采用荧光探针Mito-tracker Red观察线粒体形态,采用免疫荧光检测线粒体外膜标志蛋白TOMM20表达并检测ATP水平以分析FLO对蜕膜基质细胞线粒体功能的影响。结果:25.00μg/mL FLO暴露导致细胞形态异常,蜕膜化标志分子HOXA10表达降低(P=0.002),细胞凋亡水平增加,细胞增殖能力降低;进一步发现FLO暴露后线粒体外膜标志蛋白TOMM20表达降低(P=0.010)、线粒体形态异常和ATP水平降低(P=0.003)。结论:25.00μg/mLFLO暴露可能与线粒体功能打破mDSCs的细胞增殖、凋亡平衡,损伤蜕膜化进程有关。

关键词：氟苯尼考蜕膜化子宫内膜基质细胞线粒体

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Analysis of the ligand-binding domain of macrophage colony-stimulating receptor

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Chinese Science Bulletin 2000年第13期45卷 1191-1195页

作者： LUO Shouqing ZHENG dexian LIU Yanxin RAO Qing WU Kefu State Key Laboratory of Experimental Hematology Institute of HematologyChinese Academy of Medical SciencesPeking Union Medical CollegeTianjin 300020China State Key Laboratory of Medicial Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100005China

RNAs have been extracted from human placenta. Extracellular regions of M-CSFR, D1-3, D2-3 and D3 motifs have been amplified with RT-PCR. The proteins have been expressed in ***. Enzyme-linked immusorbent assay (EIA) shows that recombinant D1-3 possesses binding ability 3 times that of D2-3. Kd, the dissociation constant of the former is 11 nmol/L, and that of latter is 39 nmol/L. D3 lacks binding ability. These data suggest that D2-3 is the main site for M-CSF binding, D1 is an assistant site and also contributes to the conformation of site for ligand binding.

关键词： M-CSF binding ability structure-function.

Plasmon-tunable Au@Ag core-shell spiky nanoparticles for surfaceenhanced Raman scattering

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Nano Research 2019年第2期12卷 449-455页

作者： Zhulin Huang Guowen Meng Xiaoye Hu Qijun Pan dexian Huo Hongjian Zhou Yan Ke Nianqiang Wu Key Laboratory of Materials Physics and Anhui Key Laboratory of Nanomaterials and Technology Institute of Solid State Physics Chinese Academy of Sciences P. O. Box 1129 Hefei 230031 China University of Science and Technology of China Hefei 230026 China Department of Mechanical and Aerospace Engineering West Virginia University P.O. Box 6106 Morgantown WV 26506 USA

A facile synthetic approach has been developed to prepare uniform and size-tunable spiky Au@Ag core-shell nan oparticles (NPs) to tailor the localized surface plasm on res onance (LSPR) properties. The gradual assembly of small Au nano crystals allows the size of spiky Au NPs to be modulated from tens to several hundreds of nano meters by tuning the concentration of initial Au seeds and Au source;and the thick ness of the Ag shell can be adjusted with stepwise reduction of Ag(l)ions. The LSPR bands of such spiky Au@Ag core-shell NPs resemble those of pure spiky Au NP cores of similar sizes in near-infrared region, and increasing the Ag shell thickness results in a blue shift and broadening of the LSPR band in the n ear-i nfrared regi on. Additi on ally, the spiky Au@Ag core-shell NPs exhibit improved surface-e nhan ced Rama n scattering (SERS) activity as compared to the bare spiky Au NPs and spherical Ag@Au NPs. This work has offered a facile route to synthesize plasmonic metal NPs with LSPR band in 650 to 800 nm that show strong enhancement of localized electromagnetic field, which provides an effective SERS substrate for SERS imaging and detection in biological fluids and tissues.

关键词： localized surface plasmon resonanee gold silver surface-enhanced Raman spectroscopy

Geological and geoche.ical characteristics of the Baimazhai Ni-Cu-(PGE) sulphide deposit in Yunnan, China

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Chinese Journal Of Geoche.istry 2007年第4期26卷 374-383页

作者： PU Chuanjie QIN dexian NIAN Hong ZHANG Xueshu Franco PIRAJNO FAN Zhuguo Kunming University of Science and Technology Kunming 650093 China Yunnan Non-Ferrous Metals Geological Bureau Kunming 650051 China Geological Survey of Western Anstralia 100 Plain Street WA 6004 Australia

The sulphide ores of the Baimazhai deposit, although typically orthomagmatic, locally exhibit peculiar textural features and are intimately associated with hydrothermal minerals, such as biotite, amphibole and chlorite. This association suggests that the magmatic sulphide ores were subjected to hydrothermal alteration and subsequent redistribution, resulting in the observed textural features. Geoche.ically, the Baimazhai sulphide ores are enriche. in Cu, Pd and Au, which,according to previous studies, reflects the action of hydrothermal fluids. Interestingly, Ar-Ar dating yielded the plateau ages of about 160–170 Ma, which are at odds with the established Permian age of the Emeishan large igneous province. We interpreted these younger ages as due to thermal resetting during post-Permian tectonothermal events. We have proposed a model in which tectonic movements and hydrothermal fluids related to these events modified the pre-existing magmatic sulphides. Given the degree of overprint, we suggested two possible scenarios: 1) the sulphide disseminations that surround the massive magmatic ores are the result of deformation and hydrothermal alteration; and 2) there were both magmatic massive and disseminated sulphides, in which case the scale and relocation of remobilization would have been smaller, but still detectable.

关键词：白马寨矿化作用云南硫化物

Mineralogical characteristics and photocatalytic properties of natural sphalerite from China

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Journal of Environmental Sciences 2020年第3期32卷 156-166页

作者： Can Shen Xiangping Gu Bin Yang dexian Zhang Zhilin Wang Zhengxiang Shu Jeffrey Dick Anhuai Lu Key Laboratory of Metallogenic Prediction of Nonferrous Metals and Geological Environment Monitoring Ministry of Education Central South UniversityChangsha 410083China School of Geoscience and Info-physics Central South UniversityChangsha 410083China

Different natural sphalerites have a range of photocatalytic properties that can potentially be exploited for environmental remediation *** develop value in the exploitation of sphalerite,samples were collected from 19 ore deposits in China and characterized for their mineralogical and photocatalytic properties.X-ray diffraction(XRD) and electron probe micro analysis(EPMA) measurements indicated that all the natural sphalerites from various localities crystallized in cubic phases with various che.ical *** substitution of Fe for Zn ranged from 0.235% to 14.826% by weight,Mn from 0.004% to4.868%,Cu from 0.009% to 5.529% and Cd from 0.133% to 1.576%.As Fe became more abundant,the color of natural sphalerite darkened,becoming almost black;and higher Fe content was associated with stronger visible light *** spectra showed emission mainly related to S-vacancies and progressively decreasing fluorescence intensity with increasing Fe *** of the photocatalytic degradation of methyl orange indicated that the sample with the highest Cd content but moderate Fe content had the highest photocatalytic ***,the degradation of Methyl Orange(30 mg/L)attained 82.11% efficiency under visible light irradiation for 4 hr of natural sphalerite with4.262% Fe and 1.576% ***,the Fe content in sphalerite was found to contribute to the visible light absorption ability and the recombination rate of photo-generated electrons and holes,while substitution by Cd was observed to have a greater effect on the photocatalytic *** findings provide a scientific basis for the profitable utilization of base metal resources like sphalerite.

关键词： Natural sphalerite Mineralogical properties Band gap Photodegradation of Methyl Orange

酸枣仁种皮和种仁化学成分的定性及半定量分析

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色谱 2024年第3期42卷 234-244页

作者：石宇浩南易郑伟姚兰梁海珍陈晓娟宋娟张洁贾德贤王谦马百平天津中医药大学天津301617 军事科学院军事医学研究院辐射医学研究所北京100850 北京中医药大学北京100029

酸枣仁为鼠李科植物酸枣Ziziphus jujuba ***(Bunge)Hu ex ***的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据。基于超高效液相色谱-四极杆飞行时... 详细信息

酸枣仁为鼠李科植物酸枣Ziziphus jujuba ***(Bunge)Hu ex ***的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据。基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术,从种皮、种仁中共鉴定出57个化学成分。结合主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)对两者进行差异成分研究,以变量投影重要度(VIP)值>5为标准,筛选了差异成分17个,其中白桦脂酸、桦木酮酸、麦珠子酸和酸枣仁皂苷Ⅰ主要存在于种皮部位,斯皮诺素、酸枣仁皂苷A和6-阿魏酰斯皮诺素等13个化合物主要存在于种仁部位。通过超高效液相色谱-电雾式检测器(UPLC-CAD)结合反梯度补偿技术,建立半定量液相色谱指纹图谱,考察了6个不同结构类型的代表成分的响应一致性,其不同浓度下平均响应因子间的RSD值为7.04%,各化合物响应一致性良好,可用于酸枣仁的半定量表征分析;结果表明:种皮部位主要成分为白桦脂酸和油酸,其中白桦脂酸的含量约是种仁的7倍;种仁部位主要成分为斯皮诺素、酸枣仁皂苷A、亚油酸、白桦脂酸和油酸,其中斯皮诺素、酸枣仁皂苷A的含量分别是种皮的18倍和24倍。综上,本研究阐明了酸枣仁种皮、种仁的化学成分差异,明确了酸枣仁两个部位中各自的主要成分及其相对含量,为酸枣仁不同部位合理开发和利用奠定了基础。

关键词：超高效液相色谱电雾式检测器质谱差异成分酸枣仁种皮种仁半定量分析