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Plant Diversity 2017年第3期39卷 154-159页

作者： Yanyun Tan desen li Juan Hua Shihong Luo Yan liu Shenghong li State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201PR China Yunnan Key Laboratory of Natural Medidnal Chemistry Kunming 650201 PR China University of Chinese Academy of Sdences Beijing 100049 PR China

Glandular trichomes of plants produce a wide variety of secondary metabolites which are considered as major defensive chemicals. The capitate glandular trichomes of Oenothera glazioviana(Onagraceae) were collected with laser microdissection and analyzed by gas chromatography-mass spectrometry. The volatile compound 4-hydroxy-4-methylpentan-2-one(1) was identified. We found that compound 1 displays antimicrobial, insecticidal, and phytotoxic activities. These results suggest that compound 1 might function as a defensive compound in the capitate glandular trichomes of O. glazioviana against pathogens, insect herbivores, and presumably competitive plants as well.

关键词： Oenothera glazioviana Capitate glandular trichomes Laser microdissection 4-Hydroxy-4-methylpentan-2-one Defensive functions

Characterization of two chimeric sesterterpene synthases from a fungal symbiont isolated from a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum

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Chinese Chemical Letters 2023年第1期34卷 279-283页

作者： desen li Minjie Yang Rongfang Mu Shihong Luo Yuegui Chen Wenyuan li An Wang Kai Guo Yan liu Shenghong li State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China State Key Laboratory of Southwestern Chinese Medicine Resources and Innovative Institute of Chinese Medicine and PharmacyChengdu University of Traditional Chinese MedicineChengdu 611137China College of Bioscience and Biotechnology Shenyang Agricultural UniversityShenyang 110866China University of Chinese Academy of Sciences Beijing 100049China

Two chimeric sesterterpene synthases(Aa TPS1 and Aa TPS2)were functionally characterized from Alternaria alternata MB-30 isolated from the leaves of a sesterterpenoid-producing Lamiaceae plant Leucosceptrum *** TPS1 generated a 5/8/6/5 tetracyclic sesteraltererol(1)and its absolute stereochemistry was determined by X-ray crystallographic analysis of its derivative 10,11-epoxysesteraltererol(2),which enabled revision of the absolute configuration of C7 of sesterfisherol produced by Nf SS and PTTS014 characterized previously and its derivative 10,*** TPS2 produced a 5/15 bicyclic preterpestacin I(3).Site-directed mutagenesis suggested that F192 in Aa TPS1 was likely involved in controlling of the hydroxylation of C12,and eight amino acids were important for the enzyme activity of Aa TPS1 and Aa *** engineered Escherichia coli and Saccharomyces cerevisiae strains were constructed for the productions of compounds 1 and 3,and the highest titer of compound 1 reached 62.3 mg/L in shake-flask *** compounds 1 and 2 showed anti-adipogenic activity.

关键词： Sesterterpenoids Chimeric sesterterpene synthases Plant symbiotic fungi Alternaria alternata Leucosceptrum canum Anti-adipogenic activity

Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action

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Acta Pharmaceutica Sinica B 2023年第11期13卷 4638-4654页

作者： Yuegui Chen Qin Yan Yunheng Ji Xue Bai desen li Rongfang Mu Kai Guo Minjie Yang Yang Tao Jonathan Gershenzon Yan liu Shenghong li State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China State Key Laboratory of Southwestern Chinese Medicine Resources Innovative Institute of Chinese Medicine and PharmacyChengdu University of Traditional Chinese MedicineChengdu 611137China Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Max Planck Institute for Chemical Ecology Jena D-07745Germany University of Chinese Academy of Sciences Beijing 100049China

Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive ***,we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris polyphylla *** them,a 2'-O-rhamnosyltransferase and three 6'-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides,*** UDP-glycosyltransferases showed astonishing substrate promiscuity,resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds.A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular docking simulations and protein model alignments,and a mutant UGT91AH1^(Y187A)with increased catalytic efficiency was *** steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes,and 2'-O-rhamnosylation appeared to correlate with strong antifungal *** findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents.

关键词： Paris polyphylla var.yunnanensis Steroidal saponins Glycosyltransferases Sugar chain elongation Antifungal activity

Genetic structure and domestication footprints of the tusk,coat color,and ear morphology in East Chinese pigs

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Journal of Genetics and Genomics 2022年第11期49卷 1053-1063页

作者： Sumei Zheng Pan Xu Zhongping Wu Hui Zhang desen li Shaojuan liu Bingbing liu Jun Ren Hao Chen Min Huang College of Animal Science and National Engineering Research Center for Breeding Swine Industry South China Agricultural UniversityGuangzhouGuangdong 510642China School of Animal Science and Technology Jiangsu Agri-animal Husbandry Vocational CollegeTaizhouJiangsu 225300China Zhongkai University of Agriculture and Engineering GuangzhouGuangdong 510225China College of life Sciences Jiangxi Science and Technology Normal UniversityNanchangJiangxi 330013China

The domestication and artificial selection of wild boars have led to dramatic morphological and behavioral changes,especially in East Chinese(ECN)***,we provide insights into the population structure and current genetic diversity of representative ECN pig *** identify a 500-kb region containing six tooth development-relevant genes with almost completely different haplotypes between ECN pigs and Chinese wild boars or European domestic ***,the c.195A>G missense mutation in exon 2 of AMBN may cause alterations in its protein structure associated with tusk degradation in ECN *** addition,ESR1 may play an important role in the reproductive performance of ECN pigs.A major haplotype of the large lop ear-related MSRB3 gene and eight alleles in the deafness-related GRM7 gene may affect ear morphology and hearing in ECN ***,we find that the two-end black(TEB)coat color in Jinhua pigs is most likely caused by EDNRB with genetic mechanisms different from other Chinese TEB *** study identifies key loci that may be artificially selected in Chinese native pigs related to the tusk,coat color,and ear morphology,thus providing new insights into the genetic mechanisms of domesticated pigs.

关键词： East Chinese pigs Tusk degradation Fertility Large lop ear Two-end black coat color

泽泻与吴茱萸配伍前后的指标性化学成分变化研究

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中国中药杂志 2011年第2期36卷 147-149页

作者：李小艳张秋萍吴水生许文李德森福建中医药大学药学院福建福州350108

选择泽泻、吴茱萸2味药为研究对象,以HPLC为主要研究工具,研究了不同比例配伍的泽泻-吴茱萸共煎液与单煎混合液化学成分的异同,重点关注因2味药共煎煮而产生的化学成分动态变化现象,总结了化学成分动态变化与药材配伍不同比例之间的关系。

关键词：泽泻吴茱萸共煎液单煎混合液化学成分动态变化

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大数据分析在教学质量评价中的应用

现代信息科技 2019年第12期3卷 83-85页

作者：黎德森深圳市光明区光明中学

教学质量评价是学校教学决策的重要依据,同时也是改善教学质量、改革教学模式的重要指导方向。在大数据时代的发展背景下,应该引入大数据技术来增强教学质量评价的活力,真正发挥出教学质量评价的作用。同时拓宽评价的含义,完善评价体系... 详细信息

教学质量评价是学校教学决策的重要依据,同时也是改善教学质量、改革教学模式的重要指导方向。在大数据时代的发展背景下,应该引入大数据技术来增强教学质量评价的活力,真正发挥出教学质量评价的作用。同时拓宽评价的含义,完善评价体系的功能。本文以教学质量评价为研究对象,探究了传统教学质量评价存在的弊端、分析了大数据技术在教学质量评价中的应用必要性及构建原则,最后简要介绍了基于大数据的教学质量评价体系的构建方法,以期为实际的教学质量评价体系构建提供指导。

关键词：大数据分析教学质量评价数据挖掘

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氯沙坦治疗充血性心力衰竭的临床疗效观察

临床心血管病杂志 2001年第2期17卷 78-79页

作者：肖永祺李德森耿登峰黎燕清南宁市第三医院心内科南宁530003 中山医科大学附属孙逸仙纪念医院心内科

目的 :观察氯沙坦治疗充血性心力衰竭 (CHF)的临床疗效。方法 :40例经常规洋地黄、利尿剂、血管扩张药等治疗效果欠佳的 CHF患者 ,给予口服氯沙坦 2 5～ 10 0 (5 0± 15 ) mg/ d,治疗 4～ 6周。观察治疗前后心率、心胸比、血压、左室舒... 详细信息

目的 :观察氯沙坦治疗充血性心力衰竭 (CHF)的临床疗效。方法 :40例经常规洋地黄、利尿剂、血管扩张药等治疗效果欠佳的 CHF患者 ,给予口服氯沙坦 2 5～ 10 0 (5 0± 15 ) mg/ d,治疗 4～ 6周。观察治疗前后心率、心胸比、血压、左室舒张末期内径、左室射血分数以及心功能的变化。结果 :治疗后心率、血压、心胸比以及左室舒张末期内径均明显下降 (P <0 .0 5、<0 .0 1)。左室射血分数增加 (P <0 .0 1) ,心功能改善 1～ 2级。药物副作用少 ,患者耐受性好。结论 :氯沙坦治疗 CHF疗效好 ,副作用少 ,值得临床推广。

关键词：充血性心力衰竭氯沙坦血管紧张素Ⅱ 药物治疗

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冻土地区桥涵基础早期病害的防治

青海交通科技 2015年第5期27卷 51-54页

作者：史超李得森青海省湟源高速公路养护处湟源812100 青海威远路桥有限责任公司西宁810008

结合共玉公路桥涵基础施工中的总结,从基坑开挖时的冻土防治措施,基底处理的措施预防冻土破坏,模板工程和防腐措施,负温混凝土配合比的优化和施工工艺控制的措施,负温混凝土的生产和施工中的措施,冻土地基和台背回填的防治措施等七个方... 详细信息

结合共玉公路桥涵基础施工中的总结,从基坑开挖时的冻土防治措施,基底处理的措施预防冻土破坏,模板工程和防腐措施,负温混凝土配合比的优化和施工工艺控制的措施,负温混凝土的生产和施工中的措施,冻土地基和台背回填的防治措施等七个方面概述了冻土地区桥涵基础早期病害的防治。

关键词：道路工程桥涵基础冻土病害防治

黄精、陈皮、山楂提取液蒸制板栗的制备及其多糖对氧化应激小鼠血清抗氧化作用

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食品工业科技 2023年第24期44卷 193-199页

作者：檀兴慧王文义卢淑珍吴水生李德森周奕福建中医药大学药学院福建福州350122 福建中医药大学科技创新与转化中心福建福州350122

目的:获得易消化且具抗氧化作用的板栗蒸制品,促进板栗药食功能的开发与利用。方法:以黄精、陈皮、山楂提取液对板栗进行蒸制加工,总多糖含量、感官评价为指标,通过单因素实验和正交试验L9(34),优选陈皮、山楂、黄精三种药食两用中药辅... 详细信息

目的:获得易消化且具抗氧化作用的板栗蒸制品,促进板栗药食功能的开发与利用。方法:以黄精、陈皮、山楂提取液对板栗进行蒸制加工,总多糖含量、感官评价为指标,通过单因素实验和正交试验L9(34),优选陈皮、山楂、黄精三种药食两用中药辅料用量;通过炭末推进法观察蒸制前后板栗对正常小鼠小肠运动的影响;通过D-半乳糖诱导小鼠产生氧化应激,分离小鼠血清,采用酶联免疫法(EliSA)检测各组小鼠血清中过氧化氢酶(CAT)、血红素加氧酶(HO-1)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)的含量。结果:根据单因素和正交试验结果,黄精、陈皮、山楂提取液蒸制板栗的配方为:91.95%板栗、陈皮3.45%、山楂2.30%、黄精2.30%,其总多糖含量为:97.10±0.16 mg/g;炭末推进实验结果显示蒸制板栗组小鼠小肠炭末推进率高于板栗组,差异具有统计学意义(PliSA检测结果显示与空白组相比,模型组血清中CAT、HO-1、SOD、GSH-Px 4种抗氧化酶活性水平均降低(P<0.05),且MDA含量增高(P<0.05);维生素C组、板栗组、组血清中CAT、HO-1、SOD、GSH-Px水平升高(P<0.05),MDA水平降低(P<0.05),其中蒸制板栗多糖组较板栗多糖组的CAT水平更显著升高(P<0.05)。结论:本研究提供一种药食两用板栗蒸制品,较正常板栗更易消化且抗氧化抗衰老活性更强。

关键词：板栗药食两用多糖氧化应激抗氧化作用

维普期刊数据库博看期刊

课程思政引导下中药学本科专业导师制协同育人探索

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中国中医药现代远程教育 2020年第21期18卷 21-23页

作者：陈婉君李德森福建中医药大学资产管理处福建福州350122 福建中医药大学药学院福建福州350122

从中药学专业教育设计入手,探讨专业导师制在中药学专业大学生的全方位教育作用,以立德和树人两方面着手设计课程思政教育内容。专业导师制作为“课程思政”的课外延伸,更有利于挖掘大学生个人潜力,开展个性化教育,从思想上引领,服务大... 详细信息

从中药学专业教育设计入手,探讨专业导师制在中药学专业大学生的全方位教育作用,以立德和树人两方面着手设计课程思政教育内容。专业导师制作为“课程思政”的课外延伸,更有利于挖掘大学生个人潜力,开展个性化教育,从思想上引领,服务大学生成长成才。

关键词：课程思政中药学专业导师制协同育人