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人类辅助生殖技术研究的里程碑——2010年诺贝尔生理学或医学奖解读

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生物化学与生物物理进展 2010年第10期37卷 1041-1046页

作者：李大可复旦大学生命科学学院生理学和生物物理学系立人实验室上海200433

试管婴儿技术是现代医学治疗不育症的人类辅助生殖技术之一.试管婴儿技术的发展,打破了人类繁衍的自然方式和过程,是生殖医学领域的一场革命,对生命医学的发展产生了重大影响.为此,在试管婴儿技术研究中做出开创性贡献的英国科学家罗伯... 详细信息

试管婴儿技术是现代医学治疗不育症的人类辅助生殖技术之一.试管婴儿技术的发展,打破了人类繁衍的自然方式和过程,是生殖医学领域的一场革命,对生命医学的发展产生了重大影响.为此,在试管婴儿技术研究中做出开创性贡献的英国科学家罗伯特·爱德华兹,荣获2010年诺贝尔生理学或医学奖.

关键词：诺贝尔奖体外受精胚胎移植试管婴儿

Preparation of a gene-engineering mutant of bacteriorhodopsin BR-D96V and corresponding poly(vinyl alcohol)-based functional composite films

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Science Bulletin 2010年第33期 3829-3834页

作者： ZHAO YingChun1,2,MING Ming1,HONG Jie2,MA DeWang1,WU Jia1,LI QingGuo3,HUANG WeiDa2 & DING JianDong1 1 Key Laboratory of Molecular Engineering of Polymers of Ministry of Education,department of Macromolecular Science,fudan university,shanghai 200433,china 2 department of Biochemistry,school of life sciences,fudan university,shanghai 200433,china 3 department of physiology and biophysics,school of life sciences,fudan university,shanghai 200433,china

Bacteriorhodopsin(BR)exhibits,as a membrane protein in Halobacterium salinarum,unique photoresponsive behaviors,and shows promise as a functional information material.A new mutant of BR with the 96th aspartic acid replaced by valine(BR-D96V)was obtained and then a composite film of BR-D96V in a synthetic polymer matrix was prepared in this *** mutant BR-D96V was expressed in a bacterio-opsin deficient halobacterial strain(L33)by gene *** valine is very hydrophobic,this point mutant keeps the basic biological activities,namely,photoelectric and photochromic ***,the lifetime of M intermediate in the BR mutant is nearly two orders of magnitude longer than that of wild-type BR in neutral aqueous solution,which benefits its potential application as an information *** M lifetime is further significantly prolonged after embedding BR-D96V into poly(vinyl alcohol)(PVA).It was also found that BR-D96V is very sensitive to water content in comparison with wild-type BR and another BR mutant.

关键词： synthetic polymer organic photoelectric material composite film bacteriorhodopsin site-specific mutagenesis photore-sponsive protein

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新世纪中推动生物科学发展的“Bio-X”

生物物理学报 2001年第1期17卷 1-9页

作者：张志鸿复旦大学生命科学学院生理学和生物物理学系立人实验室上海200433

近二年国际上正掀起建立以生物科学为中心的交叉学科研究中心或研究所等实体的热潮。其中最引人注目的是美国斯坦福大学以诺贝尔物理学奖获得者朱棣文教授等人组建的“Bio -X”研究中心。Bio -X中的Bio为生物学 ,X泛指物理学、化学、工... 详细信息

近二年国际上正掀起建立以生物科学为中心的交叉学科研究中心或研究所等实体的热潮。其中最引人注目的是美国斯坦福大学以诺贝尔物理学奖获得者朱棣文教授等人组建的“Bio -X”研究中心。Bio -X中的Bio为生物学 ,X泛指物理学、化学、工程学、医学等其它学科。在新世纪到来之时 ,生物科学中有不少迅速发展的领域迫切需要多学科交叉共同研究和参与 ,特别是 :后基因组、基于同步辐射的结构生物学、单分子测量、纳米技术、脑科学、生物医学工程等。

关键词： Bio-X 交叉学科后基因组单分子测量脑科学物理学

Induction of Epstein-Barr Virus Lytic Replication by Recombinant Adenoviruses Expressing the Zebra Gene with EBV Specific Promoters

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 215-220页

作者： Lu CHEN Juan YIN Yi CHEN Jiang ZHONG department of Microbiology and Microbial Engineering,school of life sciences,fudan university,shanghai 200433,china department of Microbiology and Microbial Engineering School of Life Sciences Fudan University Shanghai 200433 China

The latent Epstein-Barr virus (EBV) is found in the cells of many *** example,EBVis detectable in almost all cases,and in almost all tumor cells,of non-keratinizing nasopharyngeal *** the latent virus,which will result in its lytic replication and the death of tumor cells,is a potentialapproach for the treatment of EBV-associated *** this study,three recombinant adenoviruses wereconstructed to express the Zebra gene,an EBV gene responsible for switching from the latent state to ***-specific promoters were used in order to limit Zebra expression in EBV-positive cells,andreduce the potential side *** EBV promoters used were Cp,Zp and a dual promoter combining *** Zebra protein was detected in HEK293 cells as well as the EBV-positive D98-HR1cells infected with recombinant *** EBV lyric replication early antigen,EA-D,was also detected ininfected D98-HR1,implying the initiation of lytic *** the cell viability assay,Zebra-expressingadenoviruses had little effect on EBV-negative HeLa cells,while significantly reducing the cell viability andproliferation of D98-HR1 *** results indicate that EBV virus promoters can be used in adenovirusvectors to express the Zebra gene and induce EBV lytic replication in D98-HR1 cells.

关键词： Epstein-Barr virus recombinant adenovirus lytic replication cancer

Proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

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Science china Chemistry 2010年第4期53卷 737-746页

作者： CHEN Xian Institutes for Biomedical sciences, fudan university, shanghai 200433, china department of Biochemistry & biophysics, school of Medicine, university of North Carolina-Chapel Hill, USA Institutes for Biomedical sciences Fudan University Shanghai China department of Biochemistry & biophysics School of Medicine University of North Carolina-Chapel Hill Chapel Hill USA

Cellular functions, either under the normal or pathological conditions or under different stresses, are the results of the coordinated action of multiple proteins interacting in macromolecular complexes or assemblies. The precise determination of the specific composition of protein complexes, especially using scalable and high-throughput methods, represents a systematic approach toward revealing particular cellular biological functions. In this regard, the direct profiling protein-protein interactions (PPIs) represent an efficient way to dissect functional pathways for revealing novel protein functions. In this review, we illustrate the technological evolution for the large-scale and precise identification of PPIs toward higher physiologically relevant accuracy. These techniques aim at improving the efficiency of complex pull-down, the signal specificity and accuracy in distinguishing specific PPIs, and the accuracy of identifying physiological relevant PPIs. A newly developed streamline proteomic approach for mapping the binary relationship of PPIs in a protein complex is introduced.

关键词： Chen Proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

Regulation of swelling-activated chloride channels in embryonic chick heart cells

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Cell Research 2003年第1期13卷 21-28页

作者： HUA WEI, VAN AI MEI, JIA TING SUN, HAN QING ZHOU, ZHI HONG ZHANGdepartment of physiology and biophysics, Liren Laboratory, school of life sciences, fudan university, shanghai 200433, china Fishery College, shanghai Fisheries university, shanghai 200090, china department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai China Fishery College Shanghai Fisheries University Shanghai China

Swelling-activated Cl- currents, I(ci,swell), were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I(ci,swell), Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKC, elicited the Cl~ current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I(ci,swell) in chelerythrine chloride(an inhibitor of PKC)-treated cells. Con-focal microscopy experiments using FITC-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (CB) or cytochalasin D (CD) under isosmotic condition the disruption of the F-actin integrity was observed, and I(ci,Sweii) was not elicited. With combination treatment of CB with PMA,

关键词： swelling activation myocardium chloride current F-actin phosphorylation.

Approximate solutions to infinite dimensional LQ problems over infinite time horizon

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Science china Mathematics 2006年第7期49卷 865-876页

作者： PAN Liping, ZHANG Xu & CHEN Qihong school of Mathematical sciences, fudan university, shanghai 200433, china school of Mathematics, Sichuan university, Chengdu 610064, china department of Applied Mathematics, shanghai university of Finance & Economics, shanghai 200433, china school of Mathematical sciences Fudan University Shanghai China school of Mathematics Sichuan University Chengdu China department of Applied Mathematics Shanghai University of Finance & Economics Shanghai China

This paper is addressed to develop an approximate method to solve a class of infinite dimensional LQ optimal regulator problems over infinite time horizon. Our algorithm is based on a construction of approximate solutions which solve some finite dimensional LQ optimal regulator problems over finite time horizon, and it is shown that these approximate solutions converge strongly to the desired solution in the double limit sense.

关键词： approximate solution, LQ problem, infinite dimensional, infinite time horizon.

Modern thermodynamics-New concepts based on the second law of thermodynamics

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Progress in Natural Science:Materials International 2009年第1期19卷 125-135页

作者： Jitao Wang department of Microelectronics,fudan university,shanghai 200433,china department of Microelectronics Fudan University Shanghai 200433 China

Thermodynamics is a core part of *** all scientists should have a basic knowledge of *** is a science of development,and is a viewpoint of scientific development in natural *** of thermodynamics has influence not only on natural sciences,but also on social sciences and *** concepts and definitions are very important for any discipline of science,so what is classical thermodynamics and what is modern thermodynamics have become the key points of puzzledom in *** this paper,after clarification of fundamental concept in thermodynamics,a complete basic modern classification of thermodynamics is naturally *** is suggested that extended Carnot theorem and dissipation decrease theorem,together with the laws of thermodynamics,are the most fundamental theorems in thermodynamics *** thermodynamics is a new field besides equilibrium thermodynamics belonging to the equal part of the second law of thermodynamics.

关键词： Modern thermodynamics Second law of thermodynamics Thermodynamic coupling Spiral reactions Chemical oscillation Low-pressure diamond growth

Mass spectrometry analysis of melanoma related O-glycans in sera

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Science china Chemistry 2010年第4期53卷 807-811页

作者： BAI Chen1,4, WU Gang1 & ZHAO Liang2,3 1department of life Science, fudan university, shanghai 200433, china 2department of Chemistry, fudan university, shanghai 200433, china 3Institutes of Biomedical sciences, fudan university, shanghai 200433, china 4department of Food Science, shanghai Business school, shanghai 200235, china department of life Science Fudan University Shanghai China department of Food Science Shanghai Business School Shanghai China department of Chemistry Fudan University Shanghai China Institutes of Biomedical sciences Fudan University Shanghai China

Glycomics comparison was carried out by screening melanoma serum biomarkers between C57 mice with and without B16 implanted. O-glycans were released from 10 μL sera by β-elimination, purified by Graphitized Carbon Cartridge Solid Phase Extraction (GCC-SPE) and analyzed by MALDI-QIT-TOF-MS. MS raw data were acquired and exported by Launchpad software. MATLAB was then applied for further data analysis. 10 Glycans were considered to have stable changes after B16 implantation and 5 of them were under structural analysis via MS/MS.

关键词： mass spectrometry glycomics melanoma biomarkers