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Regulation of swelling-activated chloride channels in embryonic chick heart cells

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Cell Research 2003年第1期13卷 21-28页

作者： HUA WEI, VAN AI MEI, JIA TING SUN, HAN QING ZHOU, ZHI HONG ZHANGdepartment of physiology and biophysics, Liren Laboratory, school of life sciences, fudan university, shanghai 2004.3, china Fishery College, shanghai Fisheries university, shanghai 200090, china 2Fishery College Shanghai Fisheries University Shanghai 200090 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China

Swelling-activated Cl- currents, I(ci,swell), were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I(ci,swell), Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKC, elicited the Cl~ current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I(ci,swell) in chelerythrine chloride(an inhibitor of PKC)-treated cells. Con-focal microscopy experiments using FITC-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (CB) or cytochalasin D (CD) under isosmotic condition the disruption of the F-actin integrity was observed, and I(ci,Sweii) was not elicited. With combination treatment of CB with PMA,

关键词： swelling activation myocardium chloride current F-actin phosphorylation.

Proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

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Science china Chemistry 2010年第4期53卷 737-746页

作者： CHEN Xian Institutes for Biomedical sciences, fudan university, shanghai 2004.3, china department of Biochemistry & biophysics, school of Medicine, university of North Carolina-Chapel Hill, USA 1. Institutes for Biomedical sciences Fudan University Shanghai 200433 China2. Department of Biochemistry & Biophysics School of Medicine University of North Carolina-Chapel Hill Chapel Hill USA

Cellular functions, either under the normal or pathological conditions or under different stresses, are the results of the coordinated action of multiple proteins interacting in macromolecular complexes or assemblies. The precise determination of the specific composition of protein complexes, especially using scalable and high-throughput methods, represents a systematic approach toward revealing particular cellular biological functions. In this regard, the direct profiling protein-protein interactions (PPIs) represent an efficient way to dissect functional pathways for revealing novel protein functions. In this review, we illustrate the technological evolution for the large-scale and precise identification of PPIs toward higher physiologically relevant accuracy. These techniques aim at improving the efficiency of complex pull-down, the signal specificity and accuracy in distinguishing specific PPIs, and the accuracy of identifying physiological relevant PPIs. A newly developed streamline proteomic approach for mapping the binary relationship of PPIs in a protein complex is introduced.

关键词： Chen Proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

人类辅助生殖技术研究的里程碑——2010年诺贝尔生理学或医学奖解读

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生物化学与生物物理进展 2010年第10期37卷 1041-1046页

作者：李大可复旦大学生命科学学院生理学和生物物理学系立人实验室上海200433

试管婴儿技术是现代医学治疗不育症的人类辅助生殖技术之一.试管婴儿技术的发展,打破了人类繁衍的自然方式和过程,是生殖医学领域的一场革命,对生命医学的发展产生了重大影响.为此,在试管婴儿技术研究中做出开创性贡献的英国科学家罗伯... 详细信息

试管婴儿技术是现代医学治疗不育症的人类辅助生殖技术之一.试管婴儿技术的发展,打破了人类繁衍的自然方式和过程,是生殖医学领域的一场革命,对生命医学的发展产生了重大影响.为此,在试管婴儿技术研究中做出开创性贡献的英国科学家罗伯特·爱德华兹,荣获2010年诺贝尔生理学或医学奖.

关键词：诺贝尔奖体外受精胚胎移植试管婴儿

Approximate solutions to infinite dimensional LQ problems over infinite time horizon

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Science china Mathematics 2006年第7期49卷 865-876页

作者： PAN Liping, ZHANG Xu & CHEN Qihong school of Mathematical sciences, fudan university, shanghai 2004.3, china school of Mathematics, Sichuan university, Chengdu 610064, china department of Applied Mathematics, shanghai university of Finance & Economics, shanghai 2004.3, china school of Mathematical sciences Fudan University Shanghai 200433 China school of Mathematics Sichuan University Chengdu 610064 China department of Applied Mathematics Shanghai University of Finance & Economics Shanghai 200433 China

This paper is addressed to develop an approximate method to solve a class of infinite dimensional LQ optimal regulator problems over infinite time horizon. Our algorithm is based on a construction of approximate solutions which solve some finite dimensional LQ optimal regulator problems over finite time horizon, and it is shown that these approximate solutions converge strongly to the desired solution in the double limit sense.

关键词： approximate solution LQ problem infinite dimensional infinite time hori-

Mass spectrometry analysis of melanoma related O-glycans in sera

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Science china Chemistry 2010年第4期53卷 807-811页

作者： BAI Chen1,4, WU Gang1 & ZHAO Liang2,3 1department of life Science, fudan university, shanghai 2004.3, china 2department of Chemistry, fudan university, shanghai 2004.3, china 3Institutes of Biomedical sciences, fudan university, shanghai 2004.3, china 4department of Food Science, shanghai Business school, shanghai 200235, china 1. department of life Science Fudan University Shanghai 200433 China4. Department of Food Science Shanghai Business School Shanghai 200235 China2. Department of Chemistry Fudan University Shanghai 200433 China3. Institutes of Biomedical Sciences Fudan University Shanghai 200433 China

Glycomics comparison was carried out by screening melanoma serum biomarkers between C57 mice with and without B16 implanted. O-glycans were released from 10 μL sera by β-elimination, purified by Graphitized Carbon Cartridge Solid Phase Extraction (GCC-SPE) and analyzed by MALDI-QIT-TOF-MS. MS raw data were acquired and exported by Launchpad software. MATLAB was then applied for further data analysis. 10 Glycans were considered to have stable changes after B16 implantation and 5 of them were under structural analysis via MS/MS.

关键词： mass spectrometry glycomics melanoma biomarkers

Jensen's Inequality for Backward Stochastic Differential Equations

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Chinese Annals of Mathematics,Series B 2006年第5期27卷 553-564页

作者： Long JIANG department of Mathematics, china university of Mining and Technology, Xuzhou 221008, Jiangsu, china school of Mathematical sciences, fudan university, shanghai 2004.3, china school of Mathematics and System sciences, Shandong university, Jinan 250100, china. department of Mathematics China University of Mining Technology Xuzhou 221008 Jiangsu China school of Mathematical sciences Fudan University Shanghai 200433 China school of Mathematics System sciences Shandong University Jinan 250100 China

Under the Lipschitz assumption and square integrable assumption on g, the author proves that Jensen＇s inequality holds for backward stochastic differential equations with generator g if and only if g is independent of y, g（t, 0）≡ 0 and g is super homogeneous with respect to z. This result generalizes the known results on Jensen＇s inequality for gexpectation in [4, 7-9].

关键词： Backward stochastic differential equation g-Expectation Jensen's inequality for g-expectation Jensen's inequality for BSDEs

Semantic Search among Heterogeneous Biological Databases Based on Gene Ontology

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 365-370页

作者： Shun-Liang CAO, Lei QIN1, Wei-Zhong HE1, Yang ZHONG2, Yang-Yong ZHU, and Yi-Xue LI1,3* department of Computing and Information Technology, fudan university, shanghai 2004.3, china shanghai Center for Bioinformation 1 Technology, shanghai 200235, china school of life sciences, fudan university, shanghai 2004.3, china Bioinformation Center of shanghai Institutes for Biological sciences, the Chinese Academy of sciences, shanghai 200031, china department of Computing and Information Technology Fudan University Shanghai China

Semantic search is a key issue in integration of heterogeneous biological databases. In this paper, we present a methodology for implementing semantic search in BioDW, an integrated biological data warehouse. Two tables are presented: the DB2GO table to correlate Gene Ontology (GO) annotated entries from BioDW data sources with GO, and the semantic similarity table to record similarity scores derived from any pair of GO terms. Based on the two tables, multifarious ways for semantic search are provided and the corresponding entries in heterogeneous biological databases in semantic terms can be expediently searched.

关键词： Gene Ontology semantic search BioDW

Sharpness on the Lower Bound of the lifespan of Solutions to Nonlinear Wave Equations

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Chinese Annals of Mathematics,Series B 2011年第4期32卷 521-526页

作者： Yi ZHOU 1 Wei HAN 2 1 Nonlinear Mathematical Modeling and Methods Laboratory shanghai Key Laboratory for Contem- porary Applied Mathematics school of Mathematical sciences, fudan university, shanghai 2004.3, china. 2 school of Mathematical sciences, fudan university, shanghai 2004.3, china department of Mathematics, North university of china, Taiyuan 030051, china. Nonlinear Mathematical Modeling and Methods Laboratory Shanghai Key Laboratory for Contemporary Applied Mathematics School of Mathematical Sciences Fudan University Shanghai 200433 China

This paper is devoted to proving the sharpness on the lower bound of the lifespan of classical solutions to general nonlinear wave equations with small initial data in the case n = 2 and cubic nonlinearity （see the results of T. T. Li and Y. M. Chen in 1992）. For this purpose, the authors consider the following Cauchy problem： { where □=δt^2-∑i=1n δx^^2 is the wave operator, g（x）（≡/） 0 is a smooth non-negative function with compact support, and ε 〉 0 is a small parameter. It is shown that the solution blows up in a finite time, and the lifespan T（ε） of solutions has an upper bound T（ε） ≤ exp（Aε-2） with a positive constant A independent of ε, which belongs to the same kind of the lower bound of the lifespan.

关键词： Nonlinear wave equation Cauchy problem lifespan

Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2 Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 Laboratory of Molecular Microbiology,Institute of Plant physiology and Ecology,shanghai Institutes for Biological sciences,Chinese Academy of sciences,shanghai 200032,china 2 department of Medicinal Chemistry,school of Pharmacy,fudan university,shanghai 200032,china 3 State Key Laboratory of Genetic Engineering & department of Microbiology,school of life Science,fudan university,shanghai 2004.3,china 4 Laboratory of Disease and Health Genomics,Chinese National Human Genome Center at shanghai,shanghai 201203,china Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering & department of Microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32,were *** experiments showed that the promoter of U32 glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2,located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32 pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32 pglnA in *** required GlnR,and its binding to the U32 pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32 glnR allowed usto detect the in vitro interaction between the U32 GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei