检索结果-南通市图书馆

Regulation of swelling-activated chloride channels in embryonic chick heart cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 2003年第1期13卷 21-28页

作者： HUA WEI, VAN AI MEI, JIA TING SUN, HAN QING ZHOU, ZHI HONG ZHANGdepartment of physiology and biophysics, Liren Laboratory, School of life sciences, fudan university, shanghai 2.04.3, c.ina Fishery college, {6. Fisheries university, {6. 200090, c.ina 2Fishery college Shanghai Fisheries University Shanghai 200090 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1department of physiology and biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China

Swelling-activated cl- currents, I(ci,swell), were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I(ci,swell), Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKc, elicited the cl~ current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I(ci,swell) in chelerythrine chloride(an inhibitor of PKc)-treated cells. con-focal microscopy experiments using FITc-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (cB) or cytochalasin D (cD) under isosmotic condition the disruption of the F-actin integrity was observed, and I(ci,Sweii) was not elicited. With combination treatment of cB with PMA,

关键词： swelling activation myocardium chloride current F-actin phosphorylation.

Proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science c.ina chemistry 2010年第4期53卷 737-746页

作者： cHEN Xian Institutes for Biomedical sciences, fudan university, shanghai 2.04.3, c.ina department of Biochemistry & biophysics, School of Medicine, {6. of North carolina-chapel Hill, USA 1. Institutes for Biomedical sciences Fudan University Shanghai 200433 China2. Department of Biochemistry & Biophysics School of Medicine University of North Carolina-Chapel Hill Chapel Hill USA

cellular functions, either under the normal or pathological conditions or under different stresses, are the results of the coordinated action of multiple proteins interacting in macromolecular complexes or assemblies. The precise determination of the specific composition of protein complexes, especially using scalable and high-throughput methods, represents a systematic approach toward revealing particular cellular biological functions. In this regard, the direct profiling protein-protein interactions (PPIs) represent an efficient way to dissect functional pathways for revealing novel protein functions. In this review, we illustrate the technological evolution for the large-scale and precise identification of PPIs toward higher physiologically relevant accuracy. These techniques aim at improving the efficiency of complex pull-down, the signal specificity and accuracy in distinguishing specific PPIs, and the accuracy of identifying physiological relevant PPIs. A newly developed streamline proteomic approach for mapping the binary relationship of PPIs in a protein complex is introduced.

关键词： chen Proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

维普期刊数据库

人类辅助生殖技术研究的里程碑——2010年诺贝尔生理学或医学奖解读

在线全文

学校读者我要写书评

暂无评论

生物化学与生物物理进展 2010年第10期37卷 1041-1046页

作者：李大可复旦大学生命科学学院生理学和生物物理学系立人实验室上海200433

试管婴儿技术是现代医学治疗不育症的人类辅助生殖技术之一.试管婴儿技术的发展,打破了人类繁衍的自然方式和过程,是生殖医学领域的一场革命,对生命医学的发展产生了重大影响.为此,在试管婴儿技术研究中做出开创性贡献的英国科学家罗伯... 详细信息

试管婴儿技术是现代医学治疗不育症的人类辅助生殖技术之一.试管婴儿技术的发展,打破了人类繁衍的自然方式和过程,是生殖医学领域的一场革命,对生命医学的发展产生了重大影响.为此,在试管婴儿技术研究中做出开创性贡献的英国科学家罗伯特·爱德华兹,荣获2010年诺贝尔生理学或医学奖.

关键词：诺贝尔奖体外受精胚胎移植试管婴儿

维普期刊数据库

complexity Analysis and Its Application

在线全文

学校读者我要写书评

暂无评论

communications in Nonlinear Science and Numerical Simulation 1999年第1期4卷 48-50页

作者： Jiong RUAN (department of Mathematics, fudan university, shanghai 2.04.3, c.ina) Wei LIN and Dajun XIN (department of physiology and biophysics, fudan university, shanghai 2.04.3, c.ina) department of Mathematics Fudan University Shanghai 200433 China department of physiology and biophysics Fudan University Shanghai 200433 China

In this paper, we introduce a nonlinear method named as "complexity Analysis" by which we study the chaotic and other behavior in heart rate of the patients suffer from AMI, STSDS and VPB, or normal healthy subjects. The results show that the nolinear parameter-complexity reflects the dynamic state of heart. It can be used to measure the complexity of biologic systems quantitatively. It is possible to use such a method to analyze and diagnose cardiovascular disease in clinics.

关键词： heart rate variability complexity nonlinear time series analysis

Identification and characterization of glnA Promoter and its corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2 Yu-Feng YAO~1 Ming-Xue cUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 Laboratory of Molecular Microbiology,Institute of Plant physiology and Ecology,shanghai Institutes for Biological sciences,chinese Academy of sciences,shanghai 200032,c.ina 2 {6. of Medicinal chemistry,School of Pharmacy,fudan university,shanghai 200032,c.ina 3 State Key Laboratory of Genetic Engineering & {6. of Microbiology,School of life Science,fudan university,shanghai 2.04.3,c.ina 4 Laboratory of Disease and Health Genomics,chinese National Human Genome center at shanghai,shanghai 201203,c.ina Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China department of Medicinal chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering & department of Microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32,were *** experiments showed that the promoter of U32 glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2,located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 6. nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32 pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32 pglnA in *** required GlnR,and its binding to the U32 pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32 glnR allowed usto detect the in vitro interaction between the U32 GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

Inhibitory effect of parvovirus H-1 on the formation of colonies of human hepatoma cell line in vitro and its tumors in nude mice

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 1994年第1期4卷 47-56页

作者： YAN SHANGJUN cHENGWU MA XIANHUA cHEN SHANHONG WAN ZUYU LUO(physiology and biophysics department, fudan university,shanghai 2.04.3,{6.) physiology and biophysics department Fudan University Shanghai China

The inhibitory effect of parvovirus H-1 on the colonyforming ability in vitro of QGY-7703, a cultured human hepatoma cell line, and on the formation and growth of its tumors in nude mice was studied. With higher multiplicity of infection (MOI) of H-1 given, survival of the QGY-7703 cells was found to be decreased. H-1 DNA amplification level at 30 h postinfection(p.i.) was detected to be 7.4 times higher than that at 2 h by dispersed cells assay, while the cells were delayed to enter into S *** were formed in the indicator cells (new-born human kidney cell line, NBK) by progeny H-1 virus particles released from the infected QGY-7703 cells by infectious cell center assay. The formation of tumors in nude mice by QGY-7703 cells which were injected s c at 2 h postinfection was observed to be prevented in 2 groups with given MOI 25 and 50. The tumor growth of MOI 10 group occurred at a lower exponential rate than that of control,after a 20 d latent period. It was evident that parvovirus H-1 exhibited a direct inhibitory effect on the formation and growth of human hepatoma cells in vivo as well as in vitro.

关键词： Parvovirus H-1 human hepatoma cell line colony formation nude mice inhibitory effect

Preparation of a gene-engineering mutant of bacteriorhodopsin BR-D96. and corresponding poly(vinyl alcohol)-based functional composite films

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2010年第33期 3829-3834页

作者： ZHAO Yingchun1,2,MING Ming1,HONG Jie2,MA DeWang1,WU Jia1,LI QingGuo3,HUANG WeiDa2 & DING JianDong1 1 Key Laboratory of Molecular Engineering of Polymers of Ministry of Education,department of Macromolecular Science,fudan university,shanghai 2.04.3,c.ina 2 department of Biochemistry,School of life sciences,fudan university,shanghai 2.04.3,c.ina 3 department of physiology and biophysics,School of life sciences,fudan university,shanghai 2.04.3,c.ina

Bacteriorhodopsin(BR)exhibits,as a membrane protein in Halobacterium salinarum,unique photoresponsive behaviors,and shows promise as a functional information material.A new mutant of BR with the 96.h aspartic acid replaced by valine(BR-D96.)was obtained and then a composite film of BR-D96. in a synthetic polymer matrix was prepared in this *** mutant BR-D96. was expressed in a bacterio-opsin deficient halobacterial strain(L33)by gene *** valine is very hydrophobic,this point mutant keeps the basic biological activities,namely,photoelectric and photochromic ***,the lifetime of M intermediate in the BR mutant is nearly two orders of magnitude longer than that of wild-type BR in neutral aqueous solution,which benefits its potential application as an information *** M lifetime is further significantly prolonged after embedding BR-D96. into poly(vinyl alcohol)(PVA).It was also found that BR-D96. is very sensitive to water content in comparison with wild-type BR and another BR mutant.

关键词： synthetic polymer organic photoelectric material composite film bacteriorhodopsin site-specific mutagenesis photore-sponsive protein

Bacterial Type Ⅰ Glutamine Synthetase of the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32,is the Only Enzyme Responsible for Glutamine Synthesis under Physiological conditions

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第12期 821-830页

作者： Wen-Tao PENG~(1&) Jin WANG~1 Ting WU~2 Jian-Qiang HUANG~1 Jui-Shen cHIAO~1 Guo-Ping ZHAO~(1,3,4) 1 Laboratory of Molecular Microbiology,Institute of Plant physiology and Ecology,shanghai Institutes for Biological sciences,chinese Academy of sciences,shanghai 200032,c.ina 2 {6. of Medicinal chemistry,School of Pharmacy,fudan university,shanghai 200032,c.ina 3 State Key Laboratory of Genetic Engineering/{6. of Microbiology,School of life Science,fudan university,shanghai 2.04.3,c.ina 4 Laboratory of Health and Disease Genomics,chinese National Haman Genome center at shanghai,shanghai 201203,c.ina

The structural gene for glutamine synthetase,glnA,from Amycolatopsis mediterranei U32 wascloned via screening a genomic library using the analog gene from Streptomyces *** clone wasfunctionally verified by complementing for glutamine requirement of an Escherichia coli glnA null mutantunder the control of a lac *** analysis showed an open reading frame encoding a protein of46. amino acid *** deduced amino acid sequence bears significant homologies to other bacterialtype Ⅰ glutamine synthetases,specifically,71% and 72% identical to the enzymes of *** and Myco-bacterium tuberculosis,*** of this glnA gene in *** U32 led to glutamineauxotrophy with no detectable glutamine synthetase activity in *** contrast,the cloned glnA+gene cancomplement for both phenotypes in *** thus suggested that in *** U32,the glnA geneencoding glutamine synthetase is uniquely responsible for in vivo glutamine synthesis under our laboratorydefined physiological conditions.

关键词： glnA glutamine synthetase Amycolatopsis mediterranei rifamycin SV

Ultrafast excitation relaxation in light-harvesting complex LH2 from *** 6.1

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science c.ina chemistry 2004年第3期47卷 192-199页

作者： GUO Lijun1,2, LIU Yuan3, LIU Weimin2, GUO Junhua1, XU chunhe3 & QIAN Shixiong2 1. School of Physics and Information Optoelectronics, Henan university, Kaifeng 475001, c.ina 2. Physics department, fudan university, shanghai 2.04.3, c.ina 3. {6. Institute of Plant physiology, chinese Academy of sciences, {6. 200032, c.ina 1. School of Physics and Information Optoelectronics Henan University 475001 Kaifeng China 2. Physics department Fudan University 200433 Shanghai China 3. shanghai Institute of Plant physiology Chinese Academy of Sciences 200032 Shanghai China

The energy relaxation and kinetic evolution of transient spectra of bacteriochloro- phylls (Bchls) in light-harvesting complex LH2 from Rb. sphaeroides 6.1 were investigated using femtosecond pump-probe technique. Upon 783 nm excitation, the energy at B800 Bchls ex-periences an intramolecular redistribution with 0.35 ps time constant before transferring to B850 Bchls. With tuning the excitation wavelength, the dynamical evolution of excited Bchls was clearly observed, which indicates an obvious competition between the ground state bleaching and excited state absorption (ESA) of Bchls involved and an isosbestic point near 818 nm, and also demonstrates that from the lower electronic excited state of B800 Bchls to the higher exci-tonic state of B850 Bchls is an efficient routine for energy transfer. The excitation energy in higher excitonic states of B850 Bchls relaxes rapidly to the next lowest excitonic state by inter-conversion, delocalization to adjacent molecular, populating the lowest excitonic state and the change of molecular conformation.

关键词： photosynthesis, light-harvesting complex, energy transfer, ultrafast, dynamics, purple bacteria.

维普期刊数据库