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Effects of Dan-Shao-Hua-Xian on the expression of collagen type I and III in rats with hepatic fibrosis

Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 558-563页

作者： Xiao-Xia Geng, Qin Yang, Ru-Jia Xie, Xin-Hua Luo, Cheng-Xiu Li and Ming-Liang Cheng Guizhou, China department of Infectious Diseases, Affiliated Hospital of guiyang medical college department of pathophysiology, guiyang medical college Depart- ment of Infectious Diseases, People’ s Hospital of Guizhou Province and department of Pharmacology, guiyang medical college department of Infectious Diseases Affiliated Hospital of Guiyang Medical College Guizhou 550004 China.

BACKGROUND: Hepatic fibrosis is the common patholo gical change in various chronic liver diseases,and its major cause is the imbalance between the production and degra dation of the extracellular matrix, which is mainly com posed of collagens. Dan-Shao-Hua-Xian ( DSHX) cap sule, a traditional Chinese herbal compound, has shown marked preventive effects on hepatic fibrosis in rats in our previous studies. The present study was designed to further investigate its therapeutic actions on hepatic fibrosis in rats and its possible mechanisms. METHODS: Eighty male Wistar rats were randomly divid- ed into normal control group, hepatic fibrosis group, non DSHX-treated group, low-dose-treated group, and high dose-treated group. The rat models of hepatic fibrosis were established by subcutaneous injecton of CC14 , drinking al- cohol , giving diet of hyperliprosis and hypoprotein for 8 weeks. The two DSHX-treated groups were treated respec- tively with low dose (0.5 g/kg) and high dose (1.0 g/kg) of DSHX capsule p. o. everyday for 8 weeks. At the end of the experiment, liver indexes were calculated in each group in addition to the levels of the serum hyaluronic acid and alanine aminotransferase. Their degree of hepatic fibrosis and urinary excretion of hydroxyproline and expression of collagen , were detected. RESULTS: Comparison of the indexes of the hepatic fibro- sis group and non-DSHX-treated group revealed that the liver indexes, levels of serum hyaluronic acid and alanine aminotransferase, and stage of hepatic fibrosis were all sig- nificantly reduced in the two DSHX treated groups. The urinary excretion of hydroxyproline was increased and the expression of collagen I and HI in liver tissue was lessened. These alterations were more obviously observed in the high-dose-treated group. CONCLUSION: DSHX capsule has certain therapeutic effect on hepatic fibrosis in rats.

关键词： liver fibrosis collagen hyaluronic acid hydroxyproline traditional Chinese herbs

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双肾双夹大鼠肾血管性高血压模型的制作

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心脑血管病防治 2009年第5期9卷 341-343,346页

作者：杨小慢陆德琴李贤玉张菊陈胜枝李茜李保罗贵阳医学院病理生理学教研室贵州贵阳550004

目的:探讨用自制铝夹狭窄双肾动脉(2K2C)复制大鼠肾血管性高血压模型的方法。方法:选用SD大鼠64只,随机分为假手术(sham)4周(4w)组和8周(8w)组及手术(2K2C)4w组和8w组。用无创动物血压测试仪测量各组大鼠尾动脉血压值。术后4w或8w时处... 详细信息

目的:探讨用自制铝夹狭窄双肾动脉(2K2C)复制大鼠肾血管性高血压模型的方法。方法:选用SD大鼠64只,随机分为假手术(sham)4周(4w)组和8周(8w)组及手术(2K2C)4w组和8w组。用无创动物血压测试仪测量各组大鼠尾动脉血压值。术后4w或8w时处死动物,称左心室重(LVW)和体重(BW),计算左室重量指数(LVW/BW)。结果:2K2C术后2w血压即明显升高,显著高于sham组(P<0.01),术后4w血压继续上升,血压值稳定在(157.63±14.13)mmHg,术后8w血压值高达(177.98±18.03)mmHg。sham组手术前后血压值无明显变化。2K2C-4w组和2K2C-8w组LVW、LVW/BW较同时间sham组显著增加(P<0.01)。结论:采用自制铝夹能成功复制肾血管性高血压,血压升高显著且稳定,成功率高,重复性好,方法简便易行。

关键词：肾血管性高血压高血压模型 hypertension 继发性高血压制作大鼠双肾原发性高血压

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Mechanisms for regulation of gastrin and somatostatin release from isolated rat stomach during gastric distention

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World Journal of Gastroenterology 2003年第1期9卷 129-133页

作者： Yong-Yu Li department of pathophysiology,medical college of Tongji University,Shanghai 200331,China department of pathophysiology Medical College of Tongji University Shanghai 200331 China. liyyu@***

AIM: To investigate the intragastric mechanisms forregulation of gastric neuroendocrine functions during gastricdistention in isolated vascularly perfused rat ***: Isolated vascularly perfused rat stomach wasprepared, then the gastric lumen was distended with either5,10 or 15 ml pH7 isotonic saline during a period of 20 *** the distention, the axonal blocker tetrodotoxin (TTX),the cholinergic antagonist atropine, or the putativesomatostatin-antagonist cyclo [7-aminoheptanoyl-Phe-D-Trp-Lys-Thr(Bzl)] were applied by vascular perfusion. Thereleases of gastrin and somatostatin were then examinedby ***: The graded gastricdistention caused a significantvolume-dependent decrease in gastrin secretion [-183±75 (5ml), -385±86 (10 ml) and -440±85 (15 ml) pg/20 min] and asignificant increase of somatostatin secretion [260±102 (5 ml),608±148 (10 ml) and 943±316 (15 ml) pg/20 min]. In responseto 10 ml distention, the infusion of either axonal blocker TTX(10-6 M) or cholinergic blocker atropine (10-7 M) had a similaraffect. They both attenuated the decrease of gastrin releaseby approximately 50 %, and attenuated the increase ofsomatostatin release by approximately 40 %. The infusion ofsomatostatin-antagonist cyclo [7-aminoheptanoyl-Phe-D-Trp-Lys-Thr (Bzl)] (10-6M) attenuated the decrease of gastrin releaseby about 60 %. Furthermore, combined infusion of thesomatostatin-antagonist and atropine completely abolisheddistention-induced inhibition of gastrin ***: The present data suggest that distention ofisolated rat stomach stimulates somatostatin release viacholinergic and non-cholinergic TTX-insensitive pathways. Bothsomatostatin and intrinsic cholinergic pathways are responsiblefor distention-induced inhibition of gastrin release.

关键词：胃泌素生长抑制素胃胀作用机制动物模型神经内分泌功能

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Studies on monoclonal antibody AU14-1 and immunology of uterine cervical cancer

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中国病理生理杂志 1997年第4期13卷 437-442页

作者： Wu Su-Yun, Wen Gan-Sheng department of pathophysiology, department of Tumor Immunology, Jiangxi medical college, Nanchang(330006) 江西医学院病理生理系江西南昌

The article begins with a brief description of the preparation of monoclonal antibodies against uterine cervical cancer, turning then to the long-term culture of relevant hybridoma cells and transfectant cells in serum-free media. In this article, a hypothesis of "evolutionary antigen" is advanced as supported by the presence of a common antigen of human and mouse uterine cervical cancer. In addition, application of the monoclonal antibodies to clinical diagnosis is suggested while the use of these antibodies in the treatment of experimental cancer would seem feasible.

关键词： GS cell Studies on monoclonal antibody AU14-1 and immunology of uterine cervical cancer

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Ligands of Peroxisome Proliferator-activated Receptor Inhibit Homocysteineinduced DNA Methylation of Inducible Nitric Oxide Synthase Gene

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 366-376页

作者： Yideng JIANG~(1*), Jianzhong ZHANG~1, Jiantuan XIONG~1, Jun CAO~1, Guizhong LI~1, and Shuren WANG~2 1 department of Pathology, Ningxia medical college, Yinchuan 750004. China 2 department of pathophysiology, West China college of Preclinical and Forensic medical Sciences, Sichuan University, Chengdu 610041, China department of Pathology Ningxia Medical CollegeYinchuan 750004 China department of pathophysiology West China College of Preclinical and Forensic Medical Sciences Sichuan UniversityChengdu 610041 China

Homocysteine (Hcy) is a risk factor for atherosclerosis. It is generally accepted that inducible nitric oxide synthase (iNOS) is a key enzyme in the regulation of vascular disease. The aim of the present study is to investigate the effects of peroxisome proliferator-activated receptor ligands on iNOS in the presence of Hcy in human monocytes. Foam cells, induced by oxidize low density lipoprotein (ox-LDL) and phorbol myristate acetate (PMA) in the presence of different concentrations of Hcy, clofibrate and pioglitazone in human monocytes for 4 d, were examined by oil red O staining. The activity of iNOS was detected by real-time quantitative reverse transcription-polymerase chain reaction and Western blot analysis. The capability of DNA methylation was measured by assaying endogenous C5 DNA methyltransferase (C5MTase) activity, and the iNOS promoter methylation level was determined by quantitative MethyLight assays. The results indicated that Hcy increased the activity of C5MTase and the level of iNOS gene DNA methylation, resulting in a decrease of iNOS expression. Clofibrate and pioglitazone could antagonize the Hcy effect on iNOS expression through DNA methylation, resulting in attenuation of iNOS transcription. These findings suggested that Hcy decreased the expression of iNOS by elevating iNOS DNA methylation levels, which can repress the transcription of some genes. Peroxisome proliferator-activated receptor α/γ ligands can down- regulate iNOS DNA methylation, and could be useful for preventing Hcy-induced atherosclerosis by repressing iNOS expression.

关键词： homocysteine DNA methyltransferase PPARα/γ ligand iNOS DNA methylation

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Synthesis and Anticonvulsant Activity of γ-Aminobutyric Acid Derivatives

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Chinese Chemical Letters 1998年第4期9卷 369-371页

作者： Mu YUAN Yu Guo ZHONG Qi Jie PANG(department of Pharmacy, guiyang medical college, Gujyang 550004 School of Pharmacy, West China University of medical Sciences, Chengdu 610044) department of Pharmacy Guiyang Medical College Gujyang 550004 China School of Pharmacy W. China Univ. of Medical Sciences Chengdu 610044 China

Under physiological conditions, γ-aminobutyric acid poorly crosses the blood-brain barrier. It is likely that a non-toxic derivative of γ-aminobutyric acid which enters the brain easily will have useful anticonvulsant activity. 16 derivatives of γ-aminobutyric acid with an imine link to a lipophilic carrier were prepared and tested for anticonvulsant activity; six compounds show anticonvulsant activity.

关键词： γ-aminobutyric acid synthesis anticonvulsant activity

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Effects of extract F of red-rooted Salvia on mucosal lesions of gastric corpus and antrum induced by hemorrhagic shock-reperfusion in rats

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World Journal of Gastroenterology 2001年第5期7卷 672-677页

作者： Li-Hong Zhang~1 Chang-Bai Yao~2 He-Quan Li~3 1 department of Anesthesiology,Second Clinical college,China medical University,Shenyang 110003,Liaoning Province,China2 department of General Surgery,Second Clinical college,China medical University3 department of pathophysiology,China medical University department of Anesthesiology Second Clinical College China Medical University Liaoning Province China. cumzhanglihong@***

AIM: To compare the effects of extract F of red-rooted Salvia (EFRRS) on mucosal lesions of gastric corpus and antrum induced by hemorrhagic shock and reperfusion in rats. METHODS: The rats were subject to hemorrhagic shock and followed by reperfusion, and were divided randomly into two groups. Group 1 received saline, and group 2 received EFRRS intravenously. The index of gastric mucosal lesions (IGML) was expressed as the percentage of lesional area in the corpus or antrum. The degree of gastric mucosal lesions (DGML) was catalogued grade 0,1,2 and 3. The concentrations of prostaglandins (PGs) were measured by radioimmunoassay. The concentration of MDA was measured according to the procedures of Asakawa. The activity of SOD was measured by the biochemical way. The growth rates or inhibitory rates of above-mentioned parameters were calculated. RESULTS: As compared with IGML (%), grade 3 damage (%) and MDA content (nmol/g tissue) of gastric antrum which were respectively 7.96 +/- 0.59, 34.86 +/- 4.96 and 156.98 +/- 16.12, those of gastric corpus which were respectively 23.18 +/- 6.82, 58.44 +/- 9.07 and 230.56 +/- 19.37 increased markedly (P <0.01), whereas the grade 0 damage, grade 1 damage, the concentrations of PGE(2) and PGI(2)(pg/mg tissue), the ratio of PGI(2)/TXA(2) and the activity of SOD (U/g tissue) of corpus which were respectively 3.01 +/- 1.01, 8.35 +/- 1.95, 540.48 +/- 182.78, 714.38 +/- 123.74, 17.38 +/- 5.93 and 134.29 +/- 13.35 were markedly lower than those of antrum which were respectively 13.92 +/- 2.25, 26.78 +/- 6.06, 2218.56 +/- 433.12, 2531.76 +/- 492.35, 43.46 +/- 8.51 and 187.45 +/- 17.67 (P<0.01) after hemorrhagic shock and *** intravenous EFRRS, the growth rates (%) of grade 0 damage, grade 1 damage, the concentrations of PGE(2) and PGI(2), the ratio of PGI(2)/TXA(2) and the activity of SOD of corpus which were respectively 632.56, 308.62, 40.75, 74.75, 92.29 and 122.25 were higher than those in antrum which were respe

关键词： Phytotherapy Salvia Alprostadil derivatives Animals Dinoprostone Gastric Mucosa Hydroxyl Radical Male Malondialdehyde Plant Extracts Pyloric Antrum Rats Rats, Wistar Reperfusion Injury Research Support, Non-U.S. Gov't Shock, Hemorrhagic Superoxide Dismutase Thromboxane B2

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ANGIOTENSIN Ⅱ: ANTAGONISM TO ELECTROACUPUNCTURE ANALGESIA AND INVOLVEMENT IN ELECTROACUPUNCTURE TOLERANCE IN PERIAQUEDUCTAL GREY OF THE RABBIT

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针刺研究 1989年第Z1期 145-146页

作者： Wang Xiaomin Han Jisheng department of Physiolongy, Beijing medical University,Beijing department of pathophysiology, Dalian medical college, Dalian

Male rabbits of 2. 0-2. 6kg was used. Nociception was assessed by measur- ing the latency of the escape response (ERL) elicited by strong radiant heatfocused on the skin of the nostrils. Eleetroacupuncture (EA) stimulation wasapplied via acupuncture needles inserted into the acupoint Zusanli and Qunlun,located near the knee joint and ankle joint respectively. The analgesic effectof EA (2-15Hz, 2V, 20min) could be blocked by injecting angiotensin Ⅱ (AⅡ)

关键词： PAG EA ANGIOTENSIN ANTAGONISM TO ELECTROACUPUNCTURE ANALGESIA AND INVOLVEMENT IN ELECTROACUPUNCTURE TOLERANCE IN PERIAQUEDUCTAL GREY OF THE RABBIT

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Regulation of Protein Kinase C on Proliferation and Telomerase Activity of Nasopharyngeal Carcinoma Cell Line CNE-2Z

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生物医学工程学杂志 2005年第S1期22卷 59-60页

作者： Bo BAO Pei-Chun HUANG Chuan-Ren DONG(department of pathophysiology, Guangdong medical college, Zhanjiang 524023,China)

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Different Effects of Homocysteine and Oxidized Low Density Lipoprotein on Methylation Status in the Promoter Region of the Estrogen Receptor α Gene

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Acta Biochimica et Biophysica Sinica 2007年第1期39卷 19-26页

作者： Yushan HUANG~1 Kejun PENG~2 Juan SU~1 Yuping HUANG~3 Yizhon XU~1 and Shuren WANG~1 ~1department of pathophysiology,West China School of Preclinic medical Sciences & Forensic Medicine,Sichuan University,Chengdu 610041.China ~2department of Laboratory Medicine,Chengdu medical college,Chengdu 610500,China ~3department of Biochemistry and Molecular Bilogy,Gannan medical college,Ganzhou 341000,China department of pathophysiology West China School of Preclinic Medical Sciences & Forensic Medicine Sichuan UniversityChengdu 610041 China department of Laboratory Medicine Chengdu Medical CollegeChengdu 610500 China department of Biochemistry and Molecular Biology Gannan Medical CollegeGanzhou 341000 China

We investigated the effects of homocysteine (Hcy) and oxidized low density lipoprotein (ox-LDL) on DNA methylation in the promoter region of the estrogen receptor α (ERos) gene,and its potentialmechanism in the pathogenesis of *** smooth muscle cells (SMCs) of humans weretreated by Hcy and ox-LDL with different concentrations for different periods of *** DNA methylationstatus was assayed by nested methylation-specific polymerase chain reaction,the lipids that accumulated inthe SMCs and foam cell formations were examined with Oil red O *** proliferation of SMCs wasassayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide *** results showedthat ox-LDL in moderate concentrations (10-40 mg/L) induced de novo methylation in the promoter regionof the ERα gene of ***,high concentrations (50 mg/L) of ox-LDL,resulted in demethylation ofERα.The Hcy treatment resulted in de novo methylation in the promoter region of the ERα gene with aconcentration- and treating time-dependent manner,and a dose-dependent promoting effect on *** data indicated that the two risk factors for atherosclerosis had the function of inducingde novo methylation in the promoter region of the ERα gene of SMCs. However,high concentrations (50rag/L) of ox-LDL induced demethylation,indicating that different risk factors of atherosclerosis with differentpotency might cause different aberrant methylation patterns in the promoter region of the ERα *** mechanism of Hcy might involve the hypermethylation of the ERα gene,leading to the proliferationof SMCs in atherosclerotic lesions.

关键词： atherosclerosis homocysteine oxidized low density lipoprotein estrogen receptor α DNA methylation

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