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Wnt3a signaling promotes proliferation,myogenic differentiation,and migration of rat bone marrow mesenchymal stem cells

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Acta Pharmacologica Sinica 2007年第11期28卷 1761-1774页

作者： Yan-chang SHANG~(2,3),Shu-hui WANG~4,Fu XIONG~5,Cui-ping ZHAO~2,Fu-ning PENG~2,Shan-wei FENG~2,Mei-shan LI~2,YongLI~5,Cheng ZHANG~(2,5,6)~2 department of Neurology First Affiliated hospital,Sun Yat-sen University,Guangzhou 510080,china,~3 department of Geriatric NeurologyChinese People's Liberation Army General hospital,beijing 100853,china ~4 department of Neurology,beijing friendship hospital,CapitalMedical University,beijing 100050,china,~5 Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University Guangzhou510080.china ment of Neurology First Affiliated Hospital Sun Yat-sen University Guangzhou 510080China. zhangch6@***.

Aim:To investigate the effects of the wingless-related MMTV integration site 3A(Wnt3a) signaling on the proliferation,migration,and the myogenic and adipogenicdifferentiation of rat bone marrow mesenchymal stem cells (rMSC).Methods:Primary MSC were isolated and cultured from Sprague-Dawley rats and character-ized by flow *** L cells were transfected with Wnt3a cDNA,andconditioned media containing active Wnt3a proteins were *** prolif-eration was evaluated by cell count and 5-bromodeoxyuridine incorporation *** migration of rMSC was performed by using a transwell migration and woundhealing *** myogenic and adipogenic differentiation in rMSC were exam-ined by light microscopy,immunofluorescence,and RT-PCR at different timepoints after myogenic or adipogenic ***:Wnt3a signaling in-duced β-catenin nuclear translocation and activated the Wnt pathway in *** the presence of Wnt3a,rMSC proliferated more rapidly than the control cells,keeping their differentiation ***,Wnt3a signaling induced 2.62%and 3.76% of rMSC-expressed desmin and myosin heavy chain after being cul-tured in myogenic *** myogenic differentiation genes,including Pax7,MyoD,Myf5,Myf4,and myogenin,were activated after Wnt3a *** theother hand,Wnt3a inhibited the adipogenic differentiation in rMSC through thedownregulated expression of CCAAT/enhancer-binding protein alpha (C/EBPalpha)and peroxisome proliferator-activated receptor gamma (PPARgamma).Furthermore,Wnt3a promoted the migration capacity of ***:The results indi-cate that Wnt3a signaling can induce myogenic differentiation in ***3asignaling is also involved in the regulation of the proliferation and migration *** results could provide a rational foundation for cell-based tissuerepair in humans.

关键词： Wingless-related MMTV integration site (Wnt) mesenchymal stem cells myogenic differentiation adipogenic differentiation proliferation migration

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Distribution of Inherent Strains and Residual Stressesin Medium Thickness Plate Weldment

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Journal of Materials Science & Technology 2001年第2期17卷 252-256页

作者： Peng HE and Jiuhai ZHANG National Key Laboratory of Advanced Welding Production Technology, HIT, Harbin 150001, china Toshio Terasaki and Testuya Akiyama department of Materials Science and Engineering, Kyushu Institute of Technology, japan HIT Natl Key Lab Adv Welding Prod Technol Harbin 150001 Peoples R China

A fundamental theory for the analysis of residual welding stresses and deformation based on the inherent strain distribution along the welded joint is introduced. Distribution of inherent strains and longitudinal residual stresses in medium thickness plate weldment is calculated and analyzed. A new method of calculating inherent strains and longitudinal residual stresses is proposed.

关键词： In Distribution of Inherent Strains and Residual Stressesin Medium Thickness Plate Weldment Ta

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Resistless photochemical etching of a silicon wafer by UV laser with an H_2O_2 and HF aqueous solution

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Chinese Science Bulletin 2002年第2期47卷 164-167页

作者： YANG Jie, LIU Yanfa & M. Murahara State Key Laboratory of Ultrafast Spectroscopy of Zhongshan University, Guangzhou 510275, china department of Electrical Engineering of Tokai University, japan State Key Lab. Ultrafast Spectrosc. Zhongshan University Guangzhou 510275 China

A new resistless etching method has been de-veloped for Silicon wafers. This new method uses an aqueous solution consisting of hydrogen peroxide (H2O2) and hydro-gen fluoride (HF) as the activating etchants. A 193 nm ArF excimer laser and a 266 nm fourth harmonic generation Nd:YAG laser were used as the photon sources. Results showed that pattern etching has been achieved without any photoresist film. In the case of the 193 nm laser, the optimal etching appeared at a 1.3 H2O2/HF ratio, where an etchdepth of 210 nm was achieved with a fluence of 29 mJ/cm2 and shot number of 10000. At the same conditions, the etch depth with H2O2 and HF solution was three times of that by using H2O and HF mixture. In the case of the 266 nm Nd : YAG laser, the optimal etching appeared at twice ratio of H2O2/HF, where the etch depth of 420 nm was achieved witha fluence of 12 mJ/cm2 and shot number of 30000. Results showed that the etch effect of the 266 nm Nd : YAG laser was more desirable than that of the 193 nm ArF

关键词： UV laser resistless photochemical etching hydrogen peroxide (H2O2).

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中医辨证论治及中西医结合治疗慢性化脓性中耳炎

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中国中西医结合耳鼻咽喉科杂志 2004年第6期12卷 305-307页

作者：罗克强北京中日友好医院耳鼻咽喉科 100029

目的观察辨证论治及中西医结合治疗慢性化脓性中耳炎(单纯型)的临床疗效。方法慢性化脓性中耳炎(单纯型)患者160例,随机分为三组,中医治疗组(60例)接受中药汤剂口服;西医治疗组(35例)联合口服交沙霉素和增效联磺片(SMZ+SD+TMP);中西... 详细信息

目的观察辨证论治及中西医结合治疗慢性化脓性中耳炎(单纯型)的临床疗效。方法慢性化脓性中耳炎(单纯型)患者160例,随机分为三组,中医治疗组(60例)接受中药汤剂口服;西医治疗组(35例)联合口服交沙霉素和增效联磺片(SMZ+SD+TMP);中西医结合治疗组(65例)联合服用以上中药和西药;中医治疗组仅以3％双氧水清洁耳腔,西医治疗组和中西医结合治疗组,以3％双氧水清洁耳腔后,再用0.3％氧氟沙星滴耳液进行耳浴。结果中西医结合治疗组、中医治疗组、西医治疗组治愈率分别为78.5％、45.0％、48.6％,中医治疗组和西医治疗组疗效差异无显著性意义(P>0.05),中西医结合治疗组与中医治疗组、西医治疗组疗效差异有高度显著性意义(分别P=0.0001、P=0.002)。结论中西医结合治疗慢性化脓性中耳炎(单纯型)疗效较佳。

关键词：中西医结合治疗西医治疗慢性化脓性中耳炎单纯型中医辨证论治口服疗效滴耳液显著性 SD

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Excretion of urinary glycosaminoglycans in type 2 diabetic nephropathy

Excretion of urinary glycosaminoglycans in type 2 diabetic n...

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第十一届全国中西医结合肾脏病学术会议

作者： Tingting Zhao 1,Xiaoguang Lu 1,Ping Li1,Jingzhen Guo 1,Zhiguo Li1 1.department of Pharmacology,Institute of Clinical Medical Sciences,china-japan friendship hospital,beijing,china.

Introduction Glycosaminoglycans(GAGs)are important components of plasma membranes and extra-cellular matrix,and sulfated GAGs provide polyanionic sites within the glomerular basement membrane(GBM)thus contributing to the charge selectivity of the filtration *** in GAG metabolism have been found to play a role in diabetic vascular *** in the metabolism of GAG may play an important role in the development of diabetic nephropathy.

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Carotenoid Analysis of Several Dark-Green Leafy Vegetables Associated With a Lower Risk of Cancers

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Biomedical and Environmental Sciences 1996年第4期9卷 386-392页

作者： YANG YI HUANG CHENG-YU PENG SHU-SHENG AND LI JIAN (department of Clinical Oncology, Medical Institute of Biorngulation,Kyushu University 69, Beppu 874, japan School of Public Health, West china University of Medical Sciences (WCUMS),Chengdu, china) department of Clinical Oncology Kyushu University Beppu Japan.

We have examined the carotenoid contents of several dark green vegetables found to be associated with a lower risk of various epithelial cancers in our epidemiological study and animal study. Samples of these vegetables were quantitatively examined by highperformance liquid chromatography (HPLC) on a C-18 reversed-phase column for individual carotenoid content. Pure reference compounds (alpha-carotene, beta-carotent, lycopene, canthaxanthin, and lutein) and internal standard (beta-Apo-8'-carotenal) were employed to quantify xanthophylls and carotenes in these vegetables. The results indicated that fresh, dark-green, leafy vegetables were high in beta-carotene (0.94-9.36 mg/100 g) and oxygenated carotenoids or xanthophylls, primarily lutein (0.94-7.39 mg/100 g),whereas lycopene and alpha-carotene were not prominent and canthaxanthin was non existent in these vegetables. These analyses suggest that consumption of carotenoids such as lutein in addition to beta-carotene may be associated with a lower risk of cancers

关键词： Res Am Carotenoid Analysis of Several Dark-Green Leafy Vegetables Associated With a Lower Risk of Cancers

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强直性脊柱炎骨质疏松30例临床分析

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中国实用内科杂志 2003年第1期23卷 33-35页

作者：宋淑菊马骥良北京友谊医院风湿免疫科 100050

目的　研究强直性脊柱炎 (AS)骨质疏松的发生情况、相关因素及与骨代谢指标的关系。方法　 3 0例AS患者及对照组 2 0例 ,用双能X线吸收法 (DEXA)测定腰椎、股骨颈骨密度 (BMD) ,用酶联免疫法测定血清骨钙素 (BGP)、骨碱性磷酸酶 (BAP)... 详细信息

目的　研究强直性脊柱炎 (AS)骨质疏松的发生情况、相关因素及与骨代谢指标的关系。方法　 3 0例AS患者及对照组 2 0例 ,用双能X线吸收法 (DEXA)测定腰椎、股骨颈骨密度 (BMD) ,用酶联免疫法测定血清骨钙素 (BGP)、骨碱性磷酸酶 (BAP)及尿脱氧吡啶胶原交联 (D Pyr)。结果　AS早期腰椎及股骨颈BMD均较对照组低 ,而晚期椎体周围软组织骨化使腰椎BMD增加 ,但股骨颈BMD仍低于对照组。 3 0例AS中共有 6例骨质疏松 ,10例骨量减少。AS的股骨颈BMD与病程、ESR、CRP、X线分期呈负性相关 ,绝经前女性BMD变化不如男性明显 ,HLA B2 7阳性与阴性患者BMD无明显差异。AS骨质疏松组 ,骨形成的指标BGP、BAP与对照组无明显差异 ,骨吸收的指标D Pyr明显增高。结论　AS继发全身性骨质疏松并不少见 ,其发生与病程、疾病活动指标、疾病严重程度相关 ,AS骨质疏松主要与骨吸收增加有关。

关键词：强直性脊柱炎骨质疏松临床分析

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Radiation Induced Segregation of Fe-Cr-Mn(W,V) Alloyunder Electron/Helium Dual-beam Irradiation

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International Journal of Minerals,Metallurgy and Materials 1997年第1期11卷 16-20页

作者： HU Benfu Takahashi H Ninoshita H(department of Material Science and Engineering, USTB, beijing 100083, china)(Center for Advanced Research of Energy Technology, Hokkaido University, Sapporo 060, japan)

The radiation induced segregation (RIS) was studied by electron/helium dual-beam irradiation. Within agrain, the content of Cr and Mn were higher in the center than at the margin of the irradiated areas. In thecontrary, withim the irradiated areas included grain boundaries the contents of Cr and Mn decreased at the grainboundaries. RIS was weaker under dual-beam irradiation than that under electron single-beam irradiation, whichwas because of the increases of dislocation density and sink strength of voids when helium existed.

关键词： irradiation Fe-Cr-Mn alloy segregation

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Ground state properties of La isotopes in reflection asymmetric relativistic mean field theory

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Science china(Physics,Mechanics & Astronomy) 2009年第10期52卷 1574-1578页

作者： WANG Nan1 & GUO Lu2 1 College of Physics,Shenzhen University,Shenzhen 518060,china 2 department of Physics,Faculty of Science,University of Tokyo,Tokyo 113-0033,japan College of Physics Shenzhen University Shenzhen 518060 China department of Physics Faculty of Science University of Tokyo Tokyo 113-0033 Japan

The ground state properties of La isotopes are investigated with the reflection asymmetric relativistic mean field(RAS-RMF) *** calculation results of binding energies and the quadrupole moments are in good agreements with the *** calculation results indicate the change of the quadrupole deformation with the nuclear mass *** "kink" on the isotope shifts is observed at A = 139 where the neutron number is the magic number N = *** is also found that the octupole deformations may exist in the La isotopes with mass number A ～ 145-155.

关键词： reflection asymmetric relativistic mean field(RAS-RMF) theory nuclear deformation binding energy

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Magnet Demagnetization Observation for Permanent Magnet Synchronous Motor

Magnet Demagnetization Observation for Permanent Magnet Sync...

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第11届国际电机与系统学术会议

作者： Xiao Xi Chen Changming Zhang Meng The department of Electrical Engineering Tsinghua University beijing 100084 china The department of Electrical Engineering Tsinghua University Beijing 100084 China

ISBN: (纸本)9787506292214

＜正＞Accurate information of rotor permanent magnet field is needed in permanent magnet synchronous motor（PMSM） control,but this information is variable under demagnetization conditions,such as high temperature or peak current Nonsinusoidal flux density distribution caused by demagnetization induces to torque ripple,which degrades the PMSM drive *** estimate flux magnitude,a Kalman filter is built by using stator current and PM flux as state *** estimation method can accurately track the diversity of permanent flux and is robust to motors’ parameters. The flux linkage is represented by means of Fourier series expansion,and the observe method is used for the online detection demagnetization of *** evaluations of flux linkage are carried out on a DSP-controlled PMSM drive *** results obtained demonstrate the effect of the proposed method.

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