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Binding activity of H-Ras is necessary for in vivo inhibition of ASK1 activity

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Cell Research 2004年第2期14卷 148-154页

作者： Jun DU~(1,2*), Shao Hui CAI~(3,4), Zhe SHI~3, Fumihiko NAGASE~1 ~1department of medical technology, Nagoya University School of Health Sciences, Aichi 461-8673, Japan. ~2School of Pharmaceutical Science, Sun Yat-Sen University, Guangzhou, 510089, China. ~3department of Immunology, Nagoya University Graduate and Faculty School of Medicine, Aichi, 466-8550, Japan. ~4College of Pharmacy, Jinan University, Guangzhou, 510632, China. 2School of Pharmaceutical Science Sun Yat-Sen University Guangzhou 510089 China. 4College of Pharmacy Jinan University Guangzhou 510632 China. 3department of Immunology Nagoya University Graduate and Faculty School of Medicine Aichi 466-8550 Japan. 1department of medical technology Nagoya University School of Health Sciences Aichi 461-8673 Japan.

H-Ras is well known as one of the essential components of Ras/Raf/MEK/ERK cascade, which is a critical prosurvival signaling mechanism in most eukaryotic cells. Ras targets Raf/MEK/ERK cascade by integrating and transmitting extracellular signals from growth factor receptors to Raf, leading to the propagation of signals to modulate a serious of cellular survival events. Apoptosis signal-regulating kinasel (ASK1) serves as a general mediator of cell death because it is responsive to a variety of death signals. In this study, we found that H-Ras interacted with ASK1 to cause the inhibition of both ASK1 activity and ASK1-induced apoptosis in vivo, which was reversed only partially by addition of RafS621A, an antagonist of Raf, whereas MEK inhibitor, PD98059, and PI3K inhibitor, LY294002, did not disturb the inhibitory effect of H-Ras on ASK-1-induced apoptosis. Furthermore, by means of immunoprecipitate and kinase assays, we demonstrated that the interaction between H-Ras and ASK1 as well as the inhibition

关键词： H-Ras ASK1 apoptosis binding activity.

三波长法快速测定有溶血及混浊干扰的血清胆红素含量

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华西医科大学学报 1989年第2期20卷 223-225页

作者：柴虹东云华华西医大分析测试中心波谱室华西医大公共卫生学院医学检验教研室

本文介绍一种应用三波长分光光度法于500nm、450nm、370nm处测定血清胆红素并计算其浓度。该法在≤342.0μmol/L(20mg/dl)浓度范围内,线性极好,总平均回收率96.3±2.41％(n=6)。具有抗溶血和抗混浊干扰的特点,无需特殊化学试剂,操作简便... 详细信息

本文介绍一种应用三波长分光光度法于500nm、450nm、370nm处测定血清胆红素并计算其浓度。该法在≤342.0μmol/L(20mg/dl)浓度范围内,线性极好,总平均回收率96.3±2.41％(n=6)。具有抗溶血和抗混浊干扰的特点,无需特殊化学试剂,操作简便,快速,成本低,尤其适用于临床准确测定血清游离胆红素。

关键词：胆红素血清三波长法

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地震后心理危机干预工作的思考

中国医学伦理学 2009年第4期22卷 62-63,69页

作者：杜霞王路宝鸡职业技术学院医技系检验教研室陕西宝鸡721013

对灾后心理危机干预工作包括:分析地震受害者人群,精心准备心理危机干预工作的介入,对心理危机干预工作的措施、步骤、注意问题等进行了深入的思考和总结。旨在为地震后心理危机干预工作提供支持,为灾后心理重建工作提供智力支持。

关键词：地震心理危机干预

SPR Biochip and Detection System for Tuberculosis Bacillus

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SPR Biochip and Detection System for Tuberculosis Bacillus

早期干预与TrkA受体在脑损伤修复中的作用

第2届世界华人生物医学工程研讨会

作者： Chii-Wann Lin Hsin-Chih Lai Chih-Kung Lee Shi-Ming Lin Institute of Biomedical Engineering department of Electrical Engineering department of medical technology Institute of Applied Mechanics Center of Optoelectronic Biomedicine Center for Nano Science and technology National Taiwan University Taipei Taiwan China.

Traditional Tuberculosis Bacillus (TB) diagnostic techniques, such as acid-fast stain, bacteria culture, metabolic monitoring have some disadvantages, i.e. low sensitivity, long waiting time and low

关键词： protein chip SPR Tuberculosis bacillus

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解剖学杂志 2005年第5期28卷 532-534,F0003页

作者：陈敏王玮林海陈燕惠陈达光福建医科大学医学技术与与工程学院检验系福州350004 福建医科大学神经生物学研究中心福州350004 福建医科大学附属协和医院福建省儿童神经心理发育研究室福州350004

目的:研究早期干预对缺氧缺血性脑损伤(HIBD)大鼠脑功能的影响及其与神经生长因子TrkA受体表达的关系。方法:将HIBD大鼠分为干预(H1)组和非干预(H0)组,用跳台试验检测各组大鼠学习记忆能力,并用酶免疫组织化学技术检测TrkA受体的表达情... 详细信息

目的:研究早期干预对缺氧缺血性脑损伤(HIBD)大鼠脑功能的影响及其与神经生长因子TrkA受体表达的关系。方法:将HIBD大鼠分为干预(H1)组和非干预(H0)组,用跳台试验检测各组大鼠学习记忆能力,并用酶免疫组织化学技术检测TrkA受体的表达情况。结果:H0组错误反应潜伏期较正常对照组明显缩短,而H1组的学习记忆能力则明显提高。同时,H1组额皮质和海马CA3区TrkA受体阳性神经元数目明显多于H0组。结论:早期干预方法可提高HIBD大鼠学习记忆能力,其促进脑功能修复的机制与提高脑神经细胞TrkA受体表达有关。

关键词：缺氧缺血性脑损伤早期干预 TrkA受体大鼠

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成都市区三个功能区气传真菌的定量研究

华西医科大学学报 1989年第4期20卷 448-451页

作者：杨建平殷强仲叶梅君张朝武余倩华西医大医学检验教研室

作者使用液体吸收定量采样法,于1987年在成都市三个功能区进行了一年的气传真菌检测工作。调查表明,成都市区空气中常年飘散着较高浓度的真菌孢子,浓度变化范围为870.37～7592.59个/m^3,高峰在4月和10月。经分离鉴定,气传真菌分属36属... 详细信息

作者使用液体吸收定量采样法,于1987年在成都市三个功能区进行了一年的气传真菌检测工作。调查表明,成都市区空气中常年飘散着较高浓度的真菌孢子,浓度变化范围为870.37～7592.59个/m^3,高峰在4月和10月。经分离鉴定,气传真菌分属36属和群。其中,酵母、枝孢霉属、青霉属、曲霉属、短梗霉属及无孢菌群为优势气传真菌。对空气中黄曲霉群菌株初筛结果,产毒菌占33.49％。

关键词：气传真菌功能区黄曲霉群菌

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牛膝多糖对外周血单核细胞的激活作用

华中科技大学学报（医学版） 2005年第4期34卷 413-415页

作者：宁勇姚彩萍王宇学陈家春湖北中医学院医学检验与技术系武汉430064

目的探讨牛膝多糖对外周血单核细胞是否具有激活作用。方法分别应用中性红试验检测牛膝多糖(1.250mg/ml)对单核细胞吞噬作用的影响;非特异性脂酶染色及电镜技术检测牛膝多糖(1.250mg/ml)诱导单核细胞内溶酶体的改变;0.312、1.250、5.000... 详细信息

目的探讨牛膝多糖对外周血单核细胞是否具有激活作用。方法分别应用中性红试验检测牛膝多糖(1.250mg/ml)对单核细胞吞噬作用的影响;非特异性脂酶染色及电镜技术检测牛膝多糖(1.250mg/ml)诱导单核细胞内溶酶体的改变;0.312、1.250、5.000mg/ml牛膝多糖对体外培养的外周血单核细胞进行诱导培养12h,ELISA法测定单核细胞肿瘤坏死因子α(TNFα)和白细胞介素6(IL6)的表达。结果牛膝多糖能增强单核细胞的吞噬作用,增加单核细胞胞质内溶酶体量,显著诱导单核细胞表达TNFα和IL6。结论牛膝多糖对单核细胞具有激活作用。

关键词：牛膝多糖单核细胞

Loss of C-terminal α-helix decreased SDF-1α-mediated signaling and chemotaxis without influencing CXCR4 internalization

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Acta Pharmacologica Sinica 2004年第2期25卷 26-34页

作者： Shao-hui CAI, Yi TAN2, Xian-da REN, Xiao-hong LI, Shao-xi CAI2, Jun DU3,4 department of Clinical Pharmacology, Pharmacy School of Ji-nan University, Guangzhou 510612 2College of Bioengineering, Key Laboratory for Biomechanics & Tissue Engineering of the State Ministry of Education, Chongqing University, Chongqing 400044 School of Pharmaceutical Science, Sun Yat-Sen University, Guangzhou 510612, China 3 4department of medical technology, Nagoya University School of Health Sciences, Aichi 461-8673, Japan department of Clinical Pharmacology Pharmacy School of Ji-nan University Guangzhou 510612 China

AIM: To investigate the possibility that a novel α-helix-defective mutant of stromal cell-derived factor-1α (SDF- 1α) (SDF-1/54R) acts as an antagonist of CXC chemokine receptor 4 (CXCR4). METHODS: According to the genetic sequence of natural SDF-1α, a recombinant α-helix-defective mutant of SDF-1α was designed and some biologic characteristics of this mutant were demonstrated. The migration of Jurkat cells was assessed with chemo- tactic assay. ERK phosphorylation was analyzed by Western blot with a specific anti-phospho-ERK1/2 antibody. Intracellular calcium influx was examined by flow cytometer with a calcium indicator dye Fluo-3AM. The CXCR4 on the cell surface was detected by flow cytometer with a PE conjoined anti-human CXCR4 antibody. RESULTS: Compared with native SDF-1α, SDF-1/54R displayed apparent decrease in chemotactic ability, ERK1/2 activation, and intracellular calcium influx in Jurkat cells. However, the binding to CXCR4 and inducing CXCR4 internalization of SDF-1/54R did not change outstandingly. Moreover, a competitive inhibitory effect of SDF-1/54R on the migration of Jurkat cells induced by native SDF-1α was confirmed. CONCLUSION: α-helix-defective mutant of SDF-1α, SDF-1/54R that remained both the N-terminus and the central β-sheet region, decreased SDF-1α-medi- ated signaling and chemotaxis but did not influence CXCR4 internalization, which suggested that SDF-1/54R might be developed as an anti-CHIV inhibitor with high biological potency and low side-effect.

关键词： stromal cell-derived factor-1 alpha CXC chemokine receptor 4 chemotaxis

成都地区五种家禽、家畜军团病菌的血清学调查

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华西医科大学学报 1989年第4期20卷 441-444页

作者：刘衡川殷强仲叶梅君张朝武廖骏彭启学华西医大公共卫生学院医学检验教研室

用微量凝集试验对成都地区兔、猪、鸡、鸭及鹅作嗜肺军团病菌(Lp)的血清学调查。结果除兔的Lp3型抗体未检出阳性外,其它各型的阳性率为1.27～89.8％,猪的Lp6型抗体阳性率高达89.8％,高于美国、丹麦和我国南京的报道。提示家禽、家畜中... 详细信息

用微量凝集试验对成都地区兔、猪、鸡、鸭及鹅作嗜肺军团病菌(Lp)的血清学调查。结果除兔的Lp3型抗体未检出阳性外,其它各型的阳性率为1.27～89.8％,猪的Lp6型抗体阳性率高达89.8％,高于美国、丹麦和我国南京的报道。提示家禽、家畜中可能发生军团病菌感染;对动物和外环境进行军团病的流行病学监测,对于防止人患军团病具有一定的意义。

关键词：军团杆菌病军团病杆菌家禽家畜