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KAI1 inhibits HGF-induced invasion of pancreatic cancer by sphingosine kinase activity

Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 201-208页

作者： Xu Liu,Xiao-Zhong Guo,Wei-Wei Zhang,Zhuo-Zhuang Lu,Qun-Wei Zhang, Hai-Feng Duan and Li-Sheng Wang State Key Laboratory of Cancer Biology and Institute of Digestive Diseases,Xijing hospital of Digestive Disease,Fourth Military Medical University,Xi’an 710032,china department of gastroenterology,shenyang general hospital of pla,shenyang 110016, china department of Experimental Hematology,Beijing Institute of Radiation Medicine,Beijing 100850,china [a] State Key Laboratory of Cancer Biology and Institute of Digestive Diseases Xijing Hospital of Digestive Disease Fourth Military Medical University Xi'an 710032 China [b] department of gastroenterology Shenyang General Hospital of PLA Shenyang 110016 China [c] department of Experimental Hematology Beijing Institute of Radiation Medicine Beijing 100850 China

BACKGROUND:KAI1/CD82 has been reported to attenuate the process of metastases in a variety of tumors;however,its mechanism of action in invasion has not been fully elucidated. The present study aimed to investigate the importance of KAI1 in invasion and its correlation with activation of sphingosine kinase(SPK)in human pancreatic cancer PANC1 and Miapaca-2 cell lines. METHODS:The expression of KAI1 in PANC1 and Miapaca-2 cells,which was mediated by recombinant adenovirus(Ad-KAI1), was assessed by a flow cytometer and Western *** successful infection was established,in vitro growth curve and invasive ability in Boyden Chamber assay were *** presence of KAI1 correlating with c-Met and SPK was detected by co-immunoprecipitation and[γ-32P]ATP incorporation. RESULTS:KAI1 genes had no significant effects on the curve representing cell *** infection with the KAI1 gene,decreased invasive ability in the Boyden Chamber assay was observed in PANC1 and Miapaca-2 cells that were induced by hepatocyte growth ***-expression of KAI1 in the cells led to the deactivation of SPK and a decreased level of intracellular *** correlation was observed between c-Met and KAI1 during co-immunoprecipitation. CONCLUSION:The results of this study for the first time demonstrated a regulatory role for KAI1 in SPK activation, which leads to decreased invasive ability in disease progression of human pancreatic cancer.

关键词： adenovirus vector KAI1 gene sphingosine kinase pancreatic cancer cell lines

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KAI1/CD82 suppresses hepatocyte growth factorinduced migration of hepatoma cells via upregulationof Sprouty2

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Science china(Life Sciences) 2008年第7期51卷 648-654页

作者： MU ZhenBin1 ,3,WANG Hua 2,ZHANG Jing 2,LI QingFang 2,WANG LiSheng 2&GUO XiaoZhong 3 1State Key Laboratory of Cancer Biology and Institute of Digestive Diseases,Xijing hospital,Fourth Military Medical University,Xi’an 710032,china 2department of Experimental Hematology,Beijing Institute of Radiation Medicine,Beijing 100850,china 3department of gastroenterology,shenyang general hospital of pla,shenyang 110016,china 1. State Key Laboratory of Cancer Biology and Institute of Digestive Diseases Xijing Hospital Fourth Military Medical University Xi’an 710032 China 3. department of gastroenterology Shenyang General Hospital of PLA Shenyang 110016 China 2. department of Experimental Hematology Beijing Institute of Radiation Medicine Beijing 100850 China

We conducted a study concerning the suppressive mechanism of KAI1/CD82 on hepatoma cell *** growth factor(HGF)induces the migration of hepatoma cells through activation of cellular sphingosine kinase 1(SphK1).Adenovirus-mediated gene transfer of KAI1(Ad-KAI1)downregulates the SphK1 expression and suppresses the HGF-induced migration of SMMC-7721 human hepatocellcular carcinoma *** of KAI1/CD82 significantly elevates Sprouty2 at the protein *** of Sprouty2 with RNA interference can block the KAI1/CD82-induced suppression of hepatoma cell migration and downregulation of SphK1 *** is demonstrated that KAI1/CD82 suppresses HGF-induced migration of hepatoma cells via upregulation of Sprouty2.

关键词： KAI1/CD82 Sprouty2 migration hepatocyte growth factor

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Corticosteroids or non-corticosteroids: a fresh perspective on alcoholic hepatitis treatment

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Hepatobiliary & Pancreatic Diseases International 2011年第5期10卷 458-464页

作者： Fei Wang and Bing-Yuan Wang department of gastroenterology, First Affiliated hospital, china Medical University, shenyang 110001, china department of gastroenterology First Affiliated Hospital China Medical University Shenyang 110001 China

BACKGROUND: Alcoholic hepatitis (AH) is a necrotizing inflammatory process caused by alcoholic liver injury. It carries a significant short-term mortality. The management of AH is challenging. Although corticosteroids have been demonstrated to exert anti-inflammatory and antifibrotic effects, their efficacy for the treatment of AH remains debatable. DATA SOURCES: A literature search was performed of MEDLINE, ScienceDirect, SpringerLink and Wiley InterScience using the key words 'alcoholic hepatitis', 'alcoholic liver disease', and 'corticosteroids'. The available data reported in the relevant literature were analyzed. RESULTS: More than 17 controlled trials and at least 13 meta-analyses have reported the efficacy of corticosteroids in the treatment of AH in the past 40 years. Many were poorly designed and used different inclusion/exclusion criteria, making it difficult to reach a consensus. In this review, we summarized all the controversial data in the past decade and analyzed the potential causes for the varying therapeutic effects of corticosteroids in AH. The focus of the controversy has changed from 'whether steroids are beneficial or harmful for AH patients' to 'how to accurately identify responders to steroids early and rationalize corticosteroid treatment'. An early response to glucocorticoids, as determined by calculating the Lille score after 7 days of treatment, has been shown to be a clinically useful indicator. Moreover, down-regulation of steroid sensitivity, risk of infection, and a rational therapeutic strategy of corticosteroids in AH patients are all crucial for therapeutic ***: An early and accurate determination of steroid sensitivity is important. Besides, we need to overcome the down-regulation of steroid sensitivity, reduce the infection risk and rationalize the therapeutic strategy of corticosteroids. A fresh perspective is needed on the use of corticosteroids in AH patients.

关键词： corticosteroids alcoholic liver disease alcoholic hepatitis steroid sensitivity scoring system

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Foundation and Clinical Research on the Effect of Traditional Chinese Herbs on Gastrointestinal Motility

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Chinese Journal of Integrative Medicine 2009年第2期15卷 86-88页

作者：李岩 department of gastroenterology Shengjing Hospital of China Medical University

Gastric dyskinesis functional dyspepsia is a commonly clinical condition characterized as epigastric discomfort,distension after meals,early satiety and belching,which are commonly associated with the dysfunction of g... 详细信息

关键词： Foundation and Clinical Research on the Effect of Traditional Chinese Herbs on Gastrointestinal Motility

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Cyclooxygenase-2 polymorphisms and susceptibility to gastric carcinoma: A meta-analysis

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World Journal of gastroenterology 2010年第43期16卷 5510-5517页

作者： Jia-Li Liu, Zhen-Ning Wang, department of Surgical Oncology and general Surgery, the First Affiliated hospital of china Medical University, shenyang 110001, Liaoning Province, china Yuan Liang, department of Oncology, the Shengjing Affiliated hospital of china Medical University, shenyang 110001, Liaoning Province, china Xin Zhou, department of Obstetrics and Gynecology, the Shengjing Affiliated hospital of china Medical University, shenyang 110001, Liaoning Province, china Li-Li Xing, department of Oncology, the Fourth Affiliated hospital of china Medical University, shenyang 110032, Liaoning Province, china department of Surgical Oncology and general Surgery the First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China. josieon826@***

AIM: To investigate the association of the cyclooxygenases-2 (COX-2) polymorphisms and susceptibility to gastric cancer (GC) by means of meta-analysis. METHODS: Publications addressing the association between polymorphisms of COX-2 and susceptibility to GC were selected from the MEDLINE, EMBASE and CBMdisc databases. Data was extracted from the studies by 2 independent reviewers. The meta-analyses were performed by RevMan 5.0.23. From these data, odds ratio (OR) with 95% confidence interval (CI) was ***: Ten studies were retrieved reporting a total of 11 COX-2 polymorphisms. Carriers of -765C, -1195A, -1290G, *2430T alleles and *429TT genotype revealed increased risk for GC (OR = 1.71, 95% CI: 1.01-2.90, P = 0.05; OR = 1.58, 95% CI: 1.05-2.38, P = 0.03; OR = 1.55, 95% CI: 1.01-2.39, P = 0.05; OR = 2.62, 95% CI: 1.20-5.73, P = 0.02 and OR = 0.74, 95% CI: 0.59-0.95, P = 0.02, respectively). CONCLUSION: The -765C, -1195A, -1290G, *2430T alleles and *429TT genotype of COX-2 polymorphisms were determined a significant association with susceptibility to GC.

关键词： Cyclooxygenases-2 Gastric cancer Polymorphisms Susceptibility Meta-analysis

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TGF-β_1 in retinal ganglion cells in rats with chronic ocular hypertension: its expression and anti-apoptotic effect

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International Journal of Ophthalmology(English edition) 2011年第4期4卷 396-401页

作者： Yong-Jian Tao 1, Dian-Wen Gao 1, Miao Yu 2 1department of Ophthalmology, Shengjing hospital of china Medical University, shenyang 110004, Liaoning Province, china 2 shenyang Pharmaceutical University, shenyang 110016, Liaoning Province, china department of Ophthalmology Shengjing Hospital of China Medical University Shenyang 110004 Liaoning Province China shenyang Pharmaceutical University Shenyang 110016 Liaoning Province China

AIM: To investigate the anti-apoptotic effect of transforming growth factor beta-1 (TGF-β1) on chronic ocular hypertension. METHODS: The expression of TGF-β1 in retinal ganglion cells (RCGs) was measured using the immunohistochemiscal S-P method and real-time PCR in the normally control group, the ocular hypertension group (experimental group A), the ocular hypertension plus antibody intervention group (experimental group B) and the ocular hypertension plus antigen intervention group (experimental group C) at 1, 2, 3 and 4 weeks postoperatively. The count of apoptotic RCGs was measured using the TUNEL method. RESULTS: The expression of TGF-β1 was significantly higher in experimental group C than that in other three groups (P<0.05). The expression was the lowest in experimental group B (4.17%). A statistically significant difference was noted between the four groups (P <0.01). The count of apoptotic RCGs was statistically significantly lower in experimental group C than that in the experimental groups A and B (P <0.01). A statistically significant difference was noted in the count of apoptotic RCGs between these three experimental groups (P <0.01). CONCLUSION: TGF-β1 can inhibit the apoptosis of RCGs in rats with chronic ocular hypertension.

关键词： glaucoma nerve protect TGF-β1 retinal ganglion cells chronic ocular hypertension

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Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-con-verting enzyme-1 synthesis

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Acta Pharmacologica Sinica 2007年第8期28卷 1116-1122页

作者： Chao TANG~1 An-hua WU~(1,3) Hong-li XUE~2 Yurl-jie WANG~1 ~1department of Neurosurgery,the First Affiliated hospital,china Medical University ~2department of Neurosurgery,general hospital of shenyang Military Command,shenyang 110001,china. department of Neurosurgery the First Affiliated Hospital China Medical University Shenyang 110001 China department of Neurosurgery General Hospital of Shenyang Military Command Shenyang 110001 China

Aim:To investigate the effects of tanshinone ⅡA（Tan ⅡA）on the regulation ofthe production of endothelin（ET）-1（including large ET-1）,mRNA levels of ET-1,endothelin-converting enzyme-1（ECE-1）,endothelin-A receptor（ETA）andendothelin-B receptor（ETB）induced by TNF-α in rat brain microvascular endo-thelial cells（BMVEC）.Methods:The ET-1 release（including large ET-1）into theculture medium was determined by enzyme *** levels of ET-1,ECE- 1,ETA,and ETBmRNA were measured by *** receptor bind-ing was also ***:The induction of ET-1 release by TNF-α from cul-tured BMVEC was dose-dependently reduced by Tan IIA,but large ET-1 levelsprogressively increased in response to Tan ⅡA;the mRNA expression of ET-1 *** ⅡA also caused a decrease in ETAreceptor mRNA and ECE-1expression in a dose-dependent *** receptor binding was unal-tered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and TanⅡ***:These findings suggest that Tan ⅡA may inhibit ET-1 produc-tion in TNF-α-induced BMVEC through the suppression of ECE-1 synthesis.

关键词： tanshinone IIA endothelin-1 TNF-α brain microvascular endothelial cells

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Hepatic blood inflow occlusion without hemihepatic artery control in treatment of hepatocellular carcinoma

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World Journal of gastroenterology 2010年第46期16卷 5895-5900页

作者： Shan Jin,department of general Surgery,Affiliated hospital of Inner Mongolia Medical College,Hohhot 010050,Inner Mongolia Autonomous Region,china Chao-Liu Dai,department of Hepatobiliary Surgery,Shengjing hospital,china Medical University,shenyang,Liaoning Province,china department of general Surgery Affiliated Hospital of Inner Mongolia Medical College Hohhot 010050 Inner Mongolia Autonomous Region China. jinshangood@***

AIM:To investigate the clinical significance of hepatic blood inflow occlusion without hemihepatic artery control (BIOwHAC) in the treatment of hepatocellular carcinoma (HCC).METHODS:Fifty-nine patients with HCC were divided into 3 groups based on the technique used for achieving hepatic vascular occlusion:group 1,vascular occlusion was achieved by the Pringle maneuver (n=20);group 2,by hemihepatic vascular occlusion (HVO) (n=20);and group 3,by BIOwHAC (n=19).We compared the procedures among the three groups in term of operation time,intraoperative bleeding,postoperative liver function,postoperative complications,and length of hospital ***:There were no statistically significant differences (P > 0.05) in age,sex,pathological diagnosis,preoperative Child's disease grade,hepatic function,and tumor size among the three *** intraoperative complications or deaths occurrred,and there were no significant intergroup differences (P > 0.05) in intraoperative bleeding,hepatic function change 3 and 7 d after operation,the incidence of complications,and length of hospital *** and Pringle maneuver required a significantly shorter operation time than HVO;the difference in the serum alanine aminotransferase or aspartate aminotransferase levels before and 1 d after operation was more significant in the BIOwHAC and HVO groups than in the Pringle maneuver group (P < 0.05).CONCLUSION:BIOwHAC is convenient and safe;this technique causes slight hepatic ischemia-reperfusion injury similar to HVO.

关键词： Hepatic blood inflow occlusion without hemihepatic artery control Hepatocellular carcinoma Intraoperative bleeding Ischemia-reperfusion injury

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Quantitative analysis of the ribbon synapse number of cochlear inner hair cells in C57BL/6J mice using the three-dimensional modeling method

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Science china(Life Sciences) 2009年第9期52卷 807-812页

作者： LIU Ke,LI ShuNa & JIANG XueJun department of Otolaryngology,the First Affiliated hospital,china Medical University,shenyang 110001,china department of Otolaryngology the First Affiliated Hospital China Medical University Shenyang 110001 China

In mammals,the ribbon synapses of cochlear inner hair cells are a synaptic structure of the first sensory nerve in the pathway of acoustical signal transmission to the acoustic center,and it is directly involved in voice coding and neurotransmitter release. It is difficult to quantitatively analyze the ribbon synaptic number only using an electron microscope,because the ribbon synaptic number is relatively limited and their location is deep. In this study,the specific presynaptic structure-RIBEYE,and non-specific postsynaptic structure-GluR 2 & 3 in C57BL/6J mouse basilar membrane samples were treated by immunofluorescent labeling. Serial section was performed on the samples using a laser scanning confocal microscope,and then the serial sections were used to build three-dimensional models using 3DS MAX software. Each fluorescein color pair indicates one synapse,so the number of ribbon synapses of inner hair cells is obtained. The spatial distribution and the number of ribbon synapses of cochlear inner hair cells were clearly shown in this experiment,and the mean number of ribbon synapses per inner hair cell was 16.10±1.03. Our results have demonstrated the number of ribbon synapses is accurately calculated by double immunofluorescent labeling to presynaptic and postsynaptic structures,serial sections obtained using a laser scanning confocal microscope,and three-dimensional modeling obtained using 3DS MAX software. The method above is feasible and has important significance for further exploring the mechanism of sensorineural deafness.

关键词： inner hair cells Ribbon synapses laser scanning confocal microscope three-dimensional modeling

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^(103)Pd-induced apoptosis of proliferative smooth muscle cells in bile ducts of dogs:significance and effects on related genes

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Hepatobiliary & Pancreatic Diseases International 2007年第5期6卷 521-526页

作者： Gui-Jin He,Fa-Qiang Yu,Rong Wu,Qin-Yi Gao,Shu-He Xu,Hong Gao, Wei-Guo Jiang,Tao Jiang and Xian-Wei Dai department of general Surgery,Second Affiliated hospital(Shengjing hospital),china Medical University,shenyang 110004,china,Radiotherapy Center,Second Affiliated hospital(Shengjing hospital),china Medical University,shenyang 110004,china,Pediatrics Surgery Laboratory,Second Affiliated hospital(Shengjing hospital),china Medical University,shenyang 110004,china,department of Pathology,Second Affiliated hospital(Shengjing hospital),china Medical University,shenyang 110004,china Isotope Unit,First Affiliated hospital,china Medical University,shenyang 110001,china and Isotope Research Institute,china Institute of Atomic Energy,Beijing 100050,china department of general Surgery Second Affiliated Hospital (Shengjing Hospital) China Medical University Shenyang 110004 China. 23318199@***

BACKGROUND:With the objective of developing a locally- produced radioactive stent,the present study used in vivo animal experiments to explore apoptosis of proliferative smooth muscle cells resulting from facilitation of the expression of genes caused byγ-radiation in order to prevent bile duct *** therefore explored the effects and significance ofγ-radiation on the activity of caspase-3,Fas and Bcl-2 genes in apoptosis of proliferative smooth muscle cells in the bile duct walls of dogs. METHODS:Twelve dogs were randomly divided into 2 groups(6 in each group).A postinjury bile duct stenosis model was established and radioactive 103 Pd( 103 palladium) or ordinary bile duct stents were implanted into the bile *** staining,RT-PCR and immunohistochemistry were used to detect the proliferation and apoptosis of bile duct smooth muscle cells in proliferative endomembrane and the expression of related caspase-3,Bcl-2 and Fas genes. RESULTS:The expression of caspase-3 and Fas genes in the bile duct tissues of dogs with radioactive stents was higher than that of dogs with ordinary *** was significant apoptosis of proliferative smooth muscle cells in the bile *** expression of the Bcl-2 gene in the bile duct tissues of dogs with radioactive stents was lower than that in those with ordinary *** was significant apoptosis of proliferative smooth muscle cells in the dogs with low Bcl-2 gene expression. CONCLUSIONS:Radiation increases the activity of caspase-3 and Fas genes and is associated with apoptosis. The radioactive 103 Pd stent may facilitate apoptosis of proliferative smooth muscle cells in the bile ducts of dogs by activating these *** Bcl-2 gene expression level is correlated with the occurrence of apoptosis and the radiosusceptibility of cells.

关键词： radioactive 103 Pd stent γ-radiation caspase-3 gene Fas gene Bcl-2 gene apoptosis

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