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Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain

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Journal of environmental sciences 2009年第2期21卷 218-222页

作者： WANG Wei,WEN Xianghua department of environmental science and engineering,Tsinghua University,Beijing 100084,China. department of environmental science and engineering Tsinghua University Beijing 100084 China

The lipH2 gene, encoding the expression of lignin peroxidase, was cloned from Phanerochaete chrysosporium BKM-F-1767 and expressed in Pichia pastoris X-33, a yeast. The cDNA of LiPH2 was generated from total RNA extracted from P chrysosporium by PCR with primers that do not contain a P. chrysosporium lignin peroxidase secretion signal. The gene was then successfully inserted into the expression vector pPICZα, and resulted in the recombinant vector pPICZα-lipH2. The transformation was conducted in two ways. One was using the wild Pichia pastoris as the recipients, which results in the recombinant P. pastoris with single or low lipH2 gene copy. The second was using P. pastoris and single or low lipH2 gene copy as the recipients, which results in the recombinant P. pastoris with multi-copies of lipH2 genes. This study firstly expressed the gene lipH2 in P. pastoris and achieved the successful expression of the lipH2 depending upon the generation of a recombinant strain that contained multiple copies. The lignin peroxidase activity reached a maximum of 15 U/L after 12 h induction.

关键词： heterologous expression lignin peroxidase Pichia pastoris

Quantitativ　estructure－activity　relationships　for　studying　alkyl（1－phenylsulfonyl）　cycloalkane－carboxylates

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Journal of environmental sciences 1996年第2期8卷 157-166页

作者： He Yibing Wang Liansheng (department of environmental science and engineering, Nanjing University, Nanjing 210093, China) department of environmental science Nanjing University Nanjing 210093 China

Octanol/water partition coefficients,adsorption coefficients for soils and sediments and acute toxicities to Daphnia magna of 28 alkyl (1-phenylsulfonyl) cycloalkane-carboxylates were measured. These properties were correlated by linear solvation energy relationship (LSER) and chromatographic retention data determined by reverse-phase high performance liquid chromatography (RP-HPLC). The accuracy and range of applicability of these two quantitative structure-activity relationship (QSAR) methods were compared in this paper.

关键词： alkyl (1-phenylsulfonyl) cycloalkane-carboxylates LSER R-PHPLC.

To enhance the reproduction of Phanerochaete chrysosporium by adding natural lixiviums in liquid medium

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Journal of environmental sciences 2003年第6期15卷 808-814页

作者： LIN Gang, WEN Xiang-hua, QIAN Yi (department of environmental science and engineering, Tsinghua University, Beijing 100084, China. department of environmental science and engineering Tsinghua University Beijing 100084 Chin

Great promotion to the reproduction of white rot fungus Phanerochaete chrysosporium by adding natural lixiviums such as from wood, maize core and potato in liquid medium was found in this research. Incubated in the liquid medium contained 10 mg/L glucose as carbon source with natural lixiviums for three days, the production of mycelium pellet reaches more than 80 g/L, which is 5 times more than that of without natural lixiviums. Incubated in the liquid medium contained 5 mg/L glucose as carbon source with natural lixiviums for three days, the production of mycelium pellet can reach 69.5 g/L, while the production in the medium without natural lixiviums is very low. When the liquid medium contained 1—20 g/L glucose as carbon source, the production of mycelium pellet in 3 d can only reach 12.5 g/L to 14.5 g/L. The fungus in the medium with potato lixiviums are easily contaminated by other microorganisms and in the medium with maize core lixiviums are easily bulking, while in the medium with wood lixiviums are neither easily contaminated nor bulking. Medium with wood lixiviums can produce more pellet than other medium, endure contamination and keep better sedimentation capacity. So that, wood lixivium is better additive to the culture of white rot fungi in liquid medium. Addition of the mixture of wood, maize core and potato lixiviums is of advantage to the production of mycelium pellet. The difference of the production in the medium with different amount of wood lixiviums showed little in the first 3 d, while it expanded after 3 d. Wood lixiviums stimulate the growth of P. chrysosporium instead of supply organics which fungi need.

关键词： white rot fungi medium additive

ATP as anindicator of biomass activity in thermophilic upflow anaerobic sludge blanket reactor

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Journal of environmental sciences 2000年第3期12卷 380-384页

作者： CHEN Hong (department of environmental science and engineering ,Zhejiang University ,Hangzhou 31 0 0 2 7,China) 浙江大学浙江杭州 3 1 0 0 2

Thisworkinvestigatedthebiomassactivityinathermophilicupflowanaerobicsludgeblanket (UASB )reactorof wastewatertreatment .SynthetictextilewastewaterwithpH 1 0— 1 1 ,CODlevelof2 0 0 0— 30 0 0mg/Lwastested .Cellularadenosine triphosphate (ATP)involatilesolids (VS ;mgATP/gVS)wasmeasuredandexpressedasspecificATPcontenttocomparethe biomassactivityinupzoneandlowerzoneinUASBreactor .TheresultshowsthatthespecificATPcontentbasedontotalvolatile solids (VS)inlowerzone ( 0 .0 4 6mgATP/gVSaverage)ismuchlowerthanthatinupzone ( 0 .62mgATP/gVSaverage)duetohighcontentofinactivebiomassandhighpHinlowerzone .TheSATPinupzoneincreasesasHRTincreasesandapproachestoa maximumvalueof 0 .85mgATP/gVSatHRTof 7h ,thendecreases .ItshowsmostofthetotalVSinupzonerepresentactive bacterialbiomassatHRTof 7h .Rateofsubtractutiliz*** effectofHRTonSATPinlowerzoneisnotassignificantasonSATPinupzone .ThebuffercapacityofthethermophilicUASB reactorisverygood .ItistheactivityofsludgegranulesinlowerzonethatgivetheUASBreactorsuchagoodbuffercapacitytotheinlethighpH .

关键词： ATP biomassactivity UASBreactor

INTERVAL FINITE VOLUME METHOD FOR UNCERTAINTY SIMULATION OF TWO-DIMENSIONAL RIVER WATER QUALITY

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Journal of Hydrodynamics 2004年第4期16卷 455-463页

作者： HELi ZENGGuang-ming] HUANGGuo-he LUHong-wei departmentofenvironmentalSystemengineering UniversityofReginaSaskatchewanS4S0A2Canada departmentofenvironmentalscienceandengineering HunanUniversityChangsha410082China

Under the interval uncertainties, by incorporating the discretization form offinite volume method and interval algebra theory, an Interval Finite Volume Method (IF-VM) wasdeveloped to solve water quality simulation issues for two-dimensional river when lacking effectivedata of flow velocity and flow quantity. The IFVM was practically applied to a segment of theXiangjiang River because the Project of Hunan Inland Waterway Multipurpose must be started workingafter the environmental impact assessment for it. The simulation results suggest that there existrather apparent pollution zones of BOD_5 downstream the Dongqiaogang discharger and that of CODdownstream Xiaoxiangjie discharger, but the pollution sources have no impact on the safety of thethree water plants located in this river segment. Although the developed IFVM is to be perfected, itis still a powerful tool under interval uncertainties for water environmental impact assessment,risk analysis, and water quality planning, etc. besides water quality simulation studied in thispaper.

关键词： interval finite volume method (IFVM) uncertainty simulation water quality

Effects　of　copper，manganese　and　pH　on　the　growth　and　several　enzyme　activities　of　mycorrhizal　fungus　Cenococcum　geophilum　Fr．

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Journal of environmental sciences 1996年第4期8卷 419-425页

作者： Kong Fanxiang Zhang Min(department of environmental science and engineering, Nanjing University,Nanjing 210093,China) department of environmental science Nanjing University Nanjing 210093 China

he effects of various concentrations of copper,manganese and ph on the growth,protein content and several enzyme activities of mycorrhizal fungus Cenococcum geophilum Fr. Were *** results showed that high concentration of copper (20 mg/L)and low ph (3.0-4.0) inhibited the mycelial growth (dry weight), however, the protein content increased or not significantly reduced in mycelia after copper or manganese was applied. The activities of acid phosphatase and α-mannosidase were induced by high concentration of Mn (200-400 mg/L);but the activities of G6PDH, MTLDH and trehalase were inhibited by high content of copper(10-20 mg/L),both of the responses of these enzymes and mycelial growth under the presence of copper were correlative each other. Measurement of these enzyme activities might therefore provide a useful criterion for the evaluation of the fungitoxicity of soil contaminated by copper.

关键词： copper manganese ph mycorrhizal fungus enzyme activity.

Effect of pyrolysis temperature and hold time on the characteristic parameters of adsorbent derived from sewage sludge

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Journal of environmental sciences 2004年第4期16卷 683-686页

作者： ZHAI Yun-bo, WEI Xian-xun, ZENG Guang-ming (department of environmental science and engineering,Hunan University, Changsha 410082, China. department of environmental science and engineering Human University Changsha 410082 China

According to the Doehlert’s matrix method, the adsorbent derived from sewage sludge was prepared through chemical activation under controlling the pyrolysis temperature and hold time. The characteristic parameters including the total yield, adsorption of methylene blue, adsorption of iodine, BET surface area, micro-pore volume are 35%—49%, 16.5—38 mg/g, 285—362 mg/g, 185—359 m2/g, and 0.112—0.224 m3/g, respectively. According to the experimental data, the multi-linear regression method was adopted to fit the relations between the characteristic parameters and influential factors. At final, through optimization method, the optimal adsorbent is obtained when using 62 min as hold time and 1105K as pyrolysis temperature. Under the conditions, the adsorbent was produced and compared the characteristic parameters with model forecast value, the coherence is satisfied.

关键词： adsorbent sewage sludge pyrolysis temperature hold time Doehlert’s matrix

Prediction of soot–water partition coefficients for selected persistent organic pollutants from theoretical molecular descriptors

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Progress in Natural science:Materials International 2008年第7期18卷 867-872页

作者： Qing Zhang, Jun Huang, Gang Yu * POPs Research Centre, department of environmental science and engineering, Tsinghua University, Beijing 100084, China POPs Research Centre Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China

Quantitative structure–property relationship (QSPR) models were developed for soot–water partition coefficient (KSC) values of selected persistent organic pollutants (POPs), i.e. 10 polychlorinated dibenzo-p-dioxins and dibenzofurans, nine polychlorinated biphe- nyls, four polycyclic aromatic hydrocarbons and two polybrominated diphenyl ethers, using partial least squares (PLS) regression. Quan- tum chemical descriptors computed by parameterized model revision 3 Hamiltonian method were used as predictor variables. The cross- validated Q2cum value for the optimal QSPR model is 0.844, indicating a good predictive capability for the logKSC values of these chem- icals. The QSPR results showed that average molecular polarizability (a), standard heat of formation (DHf) and energy of the lowest unoccupied molecular orbital (ELUMO) have dominant effects on KSC of POPs. The results suggested that logKSC values of POPs increase with the increase of a. Contrarily, logKSC values decrease with the increase of ELUMO and DHf of POPs.

关键词： Quantitative structure–property relationships Black carbon Persistent organic pollutants Quantum chemical descriptors Partial least squares

Estimating health damage cost from secondary sulfate particles—a case study of Hunan Province,China

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Journal of environmental sciences 2003年第5期15卷 611-617页

作者： HAO Ji ming, LI Ji, YE Xue mei, ZHU Tian le (department of environmental science and engineering, Tsinghua University, Beijing 100084, China. ) department of environmental science and engineering Tsinghua University Beijing 100084 China

China's coal dominated energy pattern has resulted in large amount of SO 2 emissions. Estimate of the sulfur related health damage cost is necessary to help perform systematic cost benefit analysis and set national energy and emissions control priorities. Current researches were confined to gaseous SO 2 in urban areas; however, secondary sulfate (SO 2- 4) particles can exert serious impact in a wider region. Based on the concept of “intake fraction', CALPUFF long range dispersion model and 180 sample emission sources, multiple regression equation was obtained with good correlation( r =0 85), which illustrates that populations were key parameters to determine intake fraction but source characteristics were insignificant. Based on the formula and the population distribution data, county level intake fractions were mapped for Hunan Province(range: 1 1×10 -6 -3 2×10 -6 ) of China. A combination of county level SO 2 emissions with the intake fractions yields a total 1 98 tons of sulfate(SO 2- 4) inhalation, and resulting total health damage cost to be 0 76(willingness to pay approach, WTP) or 0 16(human capital approach, HC) billion USD in 1997, about 2.1% or 0 45% of GDP in Hunan in 1997. Average health damage cost per ton of SO 2 emission is 930(WTP) or 200 USD(HC). The results demonstrated that more stringent regulation should be forced.

关键词： intake fraction health impact Hunan Province sulfate regression analysis