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Quantitative analysis of vascular endothelial growth factor, microvascular density and their clinicopathologic features in human hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2005年第2期4卷 220-226页

作者： Deng-Fu Yao, Xin-Hua Wu, Yong Zhu, Gong-Sheng Shi, Zhi-Zhen Dong, Deng-Bing Yao, Wei Wu, Li-Wei Qiu and Xian-Yong Meng nantong, China Research Center of Clinical Molecular Biology , department of Pathology and department of Gastroenterology , Affiliated Hos- pital of nantong university department of diagnostics , and institute of neurosciences , nantong university nantong 226001, China Research Center of Clinical Molecular Biology Affiliated Hospital of Nantong University Nantong 226001 China. dfyrcmb@***

BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful

关键词： vascular endothelial growth factor hepatocellular carcinoma ELISA microvascular density, immunohistochemistry

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Clinical impact of plasma TGF-β1 and circulating TGF-β1 mRNA in diagnosis of hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2008年第3期7卷 288-295页

作者： Zhi-Zhen Dong,Deng-Fu Yao,Min Yao,Li-Wei Qiu,Lei Zong,Wei Wu, Xin-Hua Wu,Deng-Bing Yao and Xian-Yong Meng department of diagnostics,Research Center of Clinical Molecular Biology,department of Laboratory Science,and department of GastroenterologyAffiliated Hospital and institute of neurosciences,nantong university,nantong 226001,China department of diagnostics Affiliated Hospital Nantong University Nantong 226001 China.

BACKGROUND:Transforming growth factor-β(TGF-β) plays an important role in the regulation of cell growth and differentiation,angiogenesis,extracellular matrix formation,immunosuppression and cancer development. In this study,we investigated the levels of TGF-β1 and TGF-β1 mRNA expression,their relationship with HBV replication,and their diagnostic value for hepatocellular carcinoma(HCC). METHODS:Total RNAs were extracted from HCC samples and matched non-tumor tissues,and from peripheral blood mononuclear cells in HCC ***-β1 mRNA was amplified by RT-PCR and confirmed by DNA sequencing. The distribution of TGF-β1 expression was assessed by *** clinical characteristics were analyzed between TGF-β1 and HBV *** diagnostic value of circulating TGF-β1 and TGF-β1 mRNA levels were investigated in HCC patients. RESULTS:The incidence of hepatic TGF-β1 expression was 83.3%in HCC samples,43.3%in the surrounding tissues, 94.7%in the HBV DNA-positive group,and 63.6%in the HBV DNA-negative *** TGF-β1 expression was associated with the degree of HCC differentiation and the status of HBV replication,but not with the size or number of *** TGF-β1 level and incidence of TGF-β1 mRNA were significantly higher in the HCC groupthan in any group of patients with benign liver disease, with a higher sensitivity of 89.5%and a specificity of 94.0% for HCC diagnosis when circulating TGF-β1 levels were >1.2μg/*** significant correlation was found between TGF-β1 expression and AFP level or tumor *** TGF-β1 level and serum AFP raised the detection rate to 97.4%. CONCLUSIONS:Abnormal expression of hepatic TGF-β1 is associated with the degree of HCC differentiation and HBV *** circulating TGF-β1 and TGF-β1 mRNA can be used as sensitive biomarkers for the diagnosis and prognosis of HBV-induced HCC.

关键词： hepatocellular carcinoma transforming growth factor beta immunohistochemistry diagnosis

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CONVERGENCE OF CHORIN-MARSDEN FOR MULA FOR THENAVIER-STOKES EQUATIONS ON CONVEX DOMAINS

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Journal of Computational Mathematics 1999年第1期17卷 73-88页

作者： Lung-an Ying(Research institute for Mathematical Sciences, Kyoto university, Japan department of Mathematics, Peking university, Beijing 100871, China) Peking Univ Dept Math Beijing 100871 Peoples R China Kyoto Univ Math Sci Res Inst Kyoto Japan

We prove the convergence of the Chorin-Marsden product formula for solving the initial-boundary value problems of the Navier-Stokes equations on convex domains. As a particular case we consider the case of the half pl... 详细信息

关键词： Navier Stokes equation vortex method fractional step method convergence

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Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats

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Neural Regeneration Research 2006年第7期1卷 642-644页

作者： Su Liu1, Guangyu Shen1, Guangming Lü2, Xiaosong Gu3 1department of Rehabilitation Medicine, Affiliated Hospital of nantong university, nantong 226001, Jiangsu Province, China 2department of Human Anatomy, nantong university, nantong 226001, Jiangsu Province, China 3Jiangsu Key Laboratory of Nerve Regeneration, nantong 226001, Jiangsu Province, China department of Rehabilitation Medicine Hospital of Nantong University Nantong 226001 Jiangsu Province China department of Human Anatomy Nantong University Nantong 226001 Jiangsu Province China Jiangsu Key Laboratory of Nerve Regeneration Nantong 226001 Jiangsu Province China

BACKGROUND: There are many methods for myelin staining, mordant, or oil-soluble dye or the special reaction of osmic acid with lipoid is used according to different principles. The commonly used methods are classic Well staining, classic lithium carbonate-haematine staining, fast green staining, silver staining, etc. Luxol Fast Blue can brightly stain myelin sheath, and has certain specificity. The background can be very clean if there is proper differentiation, whereas Luxol Fast Blue is cheap and convenient to operate, thus it is an ideal staining reagent for routine myelin sheath. OBJECTIVE: To show the corticospinal tract of normal adult rats with Luxol Fast Blue staining method. DESIGN: A repetitive measurement design. SETTINGS: institute of Nuerobiology, nantong university; department of Rehabilitation Medicine, Affiliated Hospital of nantong university. MATERIALS: Six healthy adult male SD rats of clean degree, weighing averagely 300 g, were provided by the experimental animal center of nantong university. 1 g/L Luxol Fast Blue solution was provided by Sigma Company; Leica CM1900 cryostat microtome by Leica Company; Leica DMR microscope by Leica Company. METHODS: The experiment was carried out in the Staff Room of Human Anatomy, nantong university in May 2005. The rats were given intraperitoneal injection of combined anesthetic (2 mL/kg), then the chest was open for perfusing saline and phosphate buffer containing formamint via heart. Brain and spinal cord were removed after 1 hour then fixed, then changed to phosphate buffer (pH 7.4) containing 300 g/L saccharu at 4 ℃, and stayed overnight, tissue blocks at pyramid, decussation of pyramid and cervical, thoracic, lumbar and sacral segments of spinal cord were removed to prepare continuous horizontal frozen sections (30 μm) after sedimentation, the sections were dried at room temperature. The corticospinal tract of normal adult rats were shown with Luxol Fast Blue staining method, and observed under Leica DMR

关键词： Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats

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Abnormal expression of HSP gp96 associated with HBV replication in human hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2006年第3期5卷 381-386页

作者： Deng-Fu Yao,Xin-Hua Wu,Xiao-Qin Su,Min Yao, Wei Wu, Li-Wei Qiu, Li Zou and Xian-Yong Meng The institute of Clinical Medicine, nantong university, and department of Gastroenterology,Affiliated Hospital of nantong university, nantong 226001, China and Nanjing Medical university, Nanjing 210009, China The institute of Clinical Medicine Nantong University Nantong 226001 China. yaodf@***

BACKGROUND: Heat shock protein (HSP) gp96 is a member of the HSP90 family and presumably overexpresses as a result of stimulation by mutated or abnormal proteins. Its abnormal expression correlates with carcinogenesis, progression and prognosis of hepatocellular carcinoma (HCC). In this study, we investigated the pathological characteristics of liver gp96 expression and its relationship with hepatitis B virus (HBV) replication in HCC patients. METHODS: Tumor specimens were prospectively collected from 30 HCC patients undergoing liver resection. Total RNAs were extracted from HCC or their noncancerous tissues. The distribution of gp96 expression in hepatocytes was investigated by streptavidin peroxidase (S-P) immunohistochemistry and tissue HBV-DNA was detected by the in situ molecular hybridization technique. The association of gp96 expression with HBV replication, and the histopathological characteristics of HCC were analyzed. RESULTS: The gp96 was strongly expressed in HCC (73.3%, 22 of 30) and weakly (46.7%, 14 of 30) in non-cancerous tissues. The gp96 expression in HCC tissues was correlated with degree of tumor differentiation and tumor size, but not with tumor number (P>0.05). Immunohistochemical analysis showed that 17 of 19 HCC patients with HBVDNA -positive were strongly expressed for gp96, whereas only 5 of 11 patients with HBV-DNA-negative were positive for gp96. A significant difference was found between the two groups (89.5% vs. 45.5%, P<0.05). CONCLUSIONS: The abnormal expressions of HSP gp96 in HCC tissues are associated with HBV replication. This finding indicates that HBV infection plays an important role in the development of HCC.

关键词： hepatocellular carcinoma heat shock protein gp96 immunohistochemistry HBV-replication

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MIXED FINITE ELEMENT METHODS FOR A STRONGLYNONLINEAR PARABOLIC PROBLEM

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Journal of Computational Mathematics 1999年第2期17卷 209-220页

作者： Yan-ping Chen(department of Mathematics, Nanjing university, Naming 210008, China institute ofComputational and Applied Mathematics, Xiangtan university, Hunan 411105, China) 南京大学数学系江苏南京

A mixed finite element method is developed to approximate the solution of a strongly nonlinear second-order parabolic problem. The existence and uniqueness of the approximation are demonstrated and L-2-error estimates... 详细信息

关键词： finite element method nonlinear parabolic problem

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Protein expression profile in the differentiation of rat bone marrow stromal cells into Schwann cell-like cells

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Science China(Life Sciences) 2009年第3期52卷 267-277页

作者： LI WenTing1,SUN HuaLin2,XU ZengLu1,DING Fei2 & GU XiaoSong2 1 department of Anatomy,Histology and Embryology,Peking Union Medical College,institute of Basic Medical Science,Chinese Academy of Medical Sciences,Beijing 100005,China 2 Jiangsu Key Laboratory of Neuroregeneration,nantong university,nantong 226001,China 1. department of Anatomy Histology and Embryology Peking Union Medical College Institute of Basic Medical Science Chinese Academy of Medical Sciences Beijing 100005 China 2. Jiangsu Key Laboratory of Neuroregeneration Nantong University Nantong 226001 China

During the last decade,increasing evidence suggested that bone marrow stromal cells(MSCs) have the potential to differentiate into neural *** studies have reported that MSCs showed morphological changes and expressed a limited number of neural proteins under experimental ***,no proteomic studies on MSCs differentiated into Schwann cell-like cells have been *** this study,we isolated MSCs from adult Sprague-Dawley rat femur and tibia bone marrows and induced the cells in vitro under specific *** using two-dimensional gel electrophoresis(2-DE),we compared the protein profiles of MSCs before and after induced *** obtained 792 protein spots in the protein profile by 2-DE,and found that 74 spots changed significantly before and after the differentiation using PDQuest software,with 43 up-regulated and 31 *** analyzed these 74 spots by a matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS) and by database searching,and found that they could be grouped into various classes,including cytoskeleton and structure proteins,growth factors,metabolic proteins,chaperone proteins,receptor proteins,cell cycle proteins,calcium binding proteins,and other *** proteins also include neural and glial proteins,such as BDNF,CNTF and *** results may provide valuable proteomic information about the differentiation of MSCs into Schwann cell-like cells.

关键词： one marrow stromal cells(MSCs) differentiation Schwann cell-like cells proteomics

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Regularity of Solutions of Certain Parabolic System with Nonstandard Growth Condition

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Acta Mathematica Sinica,English Series 1998年第2期14卷 145-160页

作者： You Jiangsheng (department of Mathematics and institute of Heavy Ion Physics Peking university,Beijing 100871,China) department of Mathematics and institute of Heavy Ion Physicn Peking Univernity

By the use of Moser iteration and Campanato space estimate,the L~∞ and C~α regularity gradient of solutions of nonlinear parabolic system (u^2)/(t)-▽(g|▽u|▽u^2)=0 with nonstandard growth conditions are obtained u... 详细信息

关键词： Nonstandard growth condition Degenerate and singular Equations Moser iteration Campanato space estimate

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Effect of intravenous injection of lipopolysaccharide and small-dose adrenaline on the opening of blood-brain barrier

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Acta Pharmacologica Sinica 1999年第5期 83-84页

作者： KUANG Fang, WANG Bai-Ren, JU Gong (department of Neuroimmunomodulation, institute of neurosciences, The Fourth Military Medical university, Xi-an 710032, China)

It is believed that in neuroimmune communication the pro-inflammatory cytokines are key messengers for immune system to influence the brain where the cascade of neural events occurred. However, the pathway that the cytokines use to signalthe brain remains a topic of *** molecular weight of cytokines is too large to pass through blood-brain barrier (BBB) *** the. present study in order to understand whether the BBB opens when the immune system is challenged,

关键词： open BBB Effect of intravenous injection of lipopolysaccharide and small-dose adrenaline on the opening of blood-brain barrier

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PHOTOINDUCED　INTRAMOLECULAR　CHARGE　TRANSFER　IN　SELF－***　AND　DUAL　FLUORESCENCE　OF　ETHYL　P－DIDECYLAMINOBENZOATE　IN　DIOXANE－WATER　BINARY　SOLVENT

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Chinese Chemical Letters 1996年第2期7卷 173-176页

作者： Yun Bac JIANG(department of Chemistry and institute of Analytical Chemistry,Xiamen university, Xiamen 361005) XIAMEN UNIV DEPT CHEMXIAMEN 361005PEOPLES R CHINA XIAMEN UNIV INST ANALYT CHEMXIAMEN 361005PEOPLES R CHINA

Aggregation of ethyl p-didecylaminobenzoate (EDDAB) in dioxane-water binary mixture was shown to occur at water volume fraction of 0.47 and a strong intramolecular charge transfer emission was observed in the aggregate.

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