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Improved two-cuff technique for orthotopic liver transplantation in rat

Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 33-37页

作者： Yong Peng, Jian-Ping Gong, Lu-Nan Yan, Shou-Bo Li and Xu-Hong Li Chongqing, china department of Hepatobiliary Surgery, Second Affilia- ted hospital, Chongqing Medical university, Chongqing 400010, china department of General Surgery, west china hospital, sichuan university, chengdu 610041 , china department of Hepatobiliary Surgery Second Affiliated Hospital Chongqing Medical University Chongqing 400010 China. pengyong725@***

BACKGROUND: The first orthotopic liver transplantatio in rat (ROLT) was reported by Lee in 1973. Kamada inno vatively applied cuff technique to ROLT in 1979. However the operative procedures were highly demanding and th operative mortality was relatively high. The purpose of thi study was to improve the model of ROLT, simplify opera tive procedures, and enhance the successful rate of opera tion. METHODS: Orthotopic liver transplantation was per formed in 160 Wistar rats by improved two-cuff technique The portal vein between donor and recipient was anasto mosed with the cuff technique. The same method was use to anastomose the infrahepatic vena cava. The suprahepati vena cava and the hepatic artery were anastomosed by m crovascular suturing and the bile duct was anastomosed en to end by a Teflon catheter. RESULTS: The average time for donor operation, graf preparation and anhepatic phase was 31 minutes, 14 mi nutes and 13 minutes, respectively. The anastomosis tim for the suprahepatic vena cava, portal vein, infrahepatic ve na cava, hepatic artery and bile duct was 7 minutes, 2 mi nutes, 2 minutes, 8 minutes and 1 minute, respectively The main causes for operative mortality were pneumotho rax, anesthesia, air embolism and massive bleeding, an the successful rate of operation was 92.5%. The causes fo death after operation were stoma bleeding, infection, b liary obstruction and graft failure. CONCLUSION: The improved two-cuff technique can re duce operative mortality, enhance survival rate, and serv as an ideal method for the establishment of animal model o ROLT.

关键词： rat liver transplantation animal, model

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Toeplitz Operators on Dirichlet Spaces

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Acta Mathematica Sinica,English Series 2001年第4期17卷 643-648页

作者： Yu Feng LU Shun Hua SUN department of Applied Mathematics, Dalian university of Technology, Dalian 116024, P. R. china department of Mathematics, sichuan university, chengdu 610064, P. R. china department of Mathematics, sichuan university, chengdu 610064, P. R. china department of Applied Mathematics Dalian University of Technology Dalian 116024 P. R. China department of Mathematics Sichuan University Chengdu 610064 P. R. China

In this paper we study Toeplitz operators on Dirichlet spaces and describe the boundedness and compactness of Toeplitz operators on Dirichlet spaces. Meanwhile, we give density theorems for Toeplitz operators on Diric... 详细信息

关键词： Dirichlet space Toeplitz operator Compact operator Strong operator topology

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Inhibitory Effect of Green Tea Extract on the Carcinogenesis Induced by Asbestos Plus Benzo(a)pyrene in Rat

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Biomedical and Environmental Sciences 1995年第1期8卷 54-58页

作者： LUO SU-QIONG LIU XUE-ZE AND WANG CHAO-JUN(department of Occupational Health, School of Public Health, west china university of Medical Sciences, chengdu 610041, china Institute of Cancer Research, west china university of Medical Sciences, chengdu 6100 department of Occupational Health School of Public Health West China University of Medical Sciences Chengdu.

In this experiment lung carcinoma was induced by crocidolite plus benzo(a)pyrene in rat. From the cancer models, we observcd that the incidcnce (16.0%) of lung carcinomas was lowel, and the survival time (376 days) of the first case of carcinoma and the mean survival time (758 days) of the rats with carcinoma were higher in the group of rats drinking 2% green tea extract for life than in the positive group (without drinking green tea extract). The mortality ratio (0.5047) was smaller in the cxperimental group than in the positive control group, and the survival curve of the experimental group significantly raised up, in comparison with the positive group.

关键词： a)pyrene in Rat Inhibitory Effect of Green Tea Extract on the Carcinogenesis Induced by Asbestos Plus Benzo Plus

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Expression of platelet endothelial cell adhesion molecule-1 between pancreatic microcirculation and peripheral circulation in rats with acute edematous pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2003年第3期2卷 463-466页

作者： Hong-Kai Gao Zong-Guang Zhou You-Qin Chen Fang-Hai Han Cun Wang the department of General Surgery and Institute of Digestive Surgery, west china hospital, sichuan university, chengdu 610041, china department of General Surgery and Institute of Digestive Surgery West China Hospital Sichuan University Chengdu 610041 China. tone257@***

OBJECTIVE: To study the ehanges of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation anti pancreatic microcirculation in rats with acute edematous pancreatitis (AEP). METHODS: The model of AEP was established with 50 Wistar rats, and the changes of PECAM-1 expression on PMNs from the splenic vein and inferior vena cava were determined by flow cytometry. RESULTS: PECAM-I expression on PMNs showed no significant difference between pancreatic microcirculation and peripheral circulation at AEP2h and AEP4h time points. From the AEP4h to the AEP8h time point, PECAM-1 expression in peripheral circulation was up-regulated, but PECAM-1 expression in pancreatic microcirculation was down-regulated. PECAM-1 expression had a significant difference between pancreatic microcirculation and peripheral circulation at the AEP8h time point (P<0.05). CONCLUSION: PECAM-1 expression on PMNs is in a converse way between pancreatic microcirculation and peripheral circulation in AEP.

关键词： acute edematous pancreatitis peripheral circulation pancretic microcirculation polymorphonuclear leukocyte platelet endothelial cell adhesion molecules-1

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Enantioselective Reduction of Prochiral Ketones Catalyed by Oxazaborolidine Derived from L-Cystine

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Chinese Chemical Letters 1997年第8期8卷 679-680页

作者： Xing Shu Li Xiao Ling Zhang Ru Gang Xie(department of Chemistry, sichuan Union university, chengdu, 610064)(department of Chemistry, sichuan Educational College, chengdu, 610041) department of Chemistry Sichuan Union University Chengdu 610064 China department of Chemistry Sichuan Educational College Chengdu 610041 China

Two new chiral oxazaborolidine derivated from L-cystine have been used to catalyze the enantioselective reduction of prochiral ketones and the secondary alcohols are obtained with good to excellent opitical yields.

关键词： Enantioselective Reduction of Prochiral Ketones Catalyed by Oxazaborolidine Derived from L-Cystine

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Studying　the　Mode－Spectra　Characteristics　of　An　Above－Threshold　Biased　Semiconductor　Laser

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Chinese Journal of Lasers 1994年第1期B3卷 11-16页

作者： XIA Guangqiong WU Zhengmao CHEN Jianguo LU Yucun(Optoelectronics department, sichuan university,chengdu 610064, china) sichuan Univ Chengdu China

ＳｔｕｄｙｉｎｇｔｈｅＭｏｄｅ－ＳｐｅｃｔｒａＣｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆＡｎＡｂｏｖｅ－ＴｈｒｅｓｈｏｌｄＢｉａｓｅｄＳｅｍｉｃｏｎｄｕｃｔｏｒＬａｓｅｒ￥ＸＩＡＧｕａｎｇｑｉｏｎｇ；ＷＵＺｈｅｎｇｍａｏ；Ｃ... 详细信息

ＳｔｕｄｙｉｎｇｔｈｅＭｏｄｅ－ＳｐｅｃｔｒａＣｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆＡｎＡｂｏｖｅ－ＴｈｒｅｓｈｏｌｄＢｉａｓｅｄＳｅｍｉｃｏｎｄｕｃｔｏｒＬａｓｅｒ￥ＸＩＡＧｕａｎｇｑｉｏｎｇ；ＷＵＺｈｅｎｇｍａｏ；ＣＨＥＮＪｉａｎｇｕｏ；ＬＵＹｕｃｕ...

关键词： semiconductor laser ray trace method mode suppression ratio carrier density

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Antisense oligonucleotides-induced local blockade of T-bet expression leads to airway inflammation in rats

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Acta Pharmacologica Sinica 2006年第5期27卷 561-567页

作者： Gang WANG~2 Chun-tao LIU~(3,4) Zeng-li WANG~3 Li-li JIANG~5 Cun-liang YAN~6 Feng-min LUO~3 ~2department of Integrated Traditional Chinese and western Medicine.west china hospital.sichuan university,chengdu 610041,china.~3department of Respiratory Medicine,west china hospital,sichuan university.chengdu 610041.china ~5department of Pathology,west china hospital.sichuan university,chengdu 610041,china:~6department of Laboratory Medicine.Shenzhen hospital,Peking university.Shenzhen 518036,china departmentoflntegratedTraditionalChineseandwesternMedicine WestChinaHospitalSichuanUniversityChengdu610041China departmentofRespiratoryMedicine WestChinaHospitalSichuanUniversityChengdu610041China

Aim:To explore whether local blockade of T-box expressed in T cells (T-bet)expression in the lungs could lead to airway ***:Twenty-fourrats were randomly divided into 4 groups:saline group,ovalbumin (OVA)-sensi-tized group,nonsense group,and the antisense *** OVA-sensitized ratswere sensitized and challenged with OVA,and the rats in the nonsense andantisense groups were subjected to an aerosol delivery of the nonsense andantisense oligonucleotides (AS-ODN) of T-bet (0.1%,w/v).The levels of inter-feron-γ(IFN-γ),interleukin-4(IL-4),and IL-5 in the bronchoalveolar lavage fluid(BALF) were detected by ELISA,and the mRNA and the protein expression ofT-bet and GATA-3 genes were examined by in situ hybridization and western blotanalysis,***:The expression of T-bet mRNA and protein in thelungs of the rats in the antisense group were inhibited *** lungs ofthe rats in the antisense and OVA-sensitized groups showed eosinophil and lym-phocyte inflammatory infiltration,and eosinophilia located predominantly aroundthe *** number of GATA-3 mRNA-positive cells and the level of GATA-3protein in the lungs of the rats in the antisense and the OVA-sensitized groupssignificantly *** level of IL-4 and IL-5 in the BALF in the antisenseand OVA-sensitized groups were elevated,but the level of IFN-γ ***:Antisense ODN-induced local blockade of T-bet expres-sion leads to airway inflammation with a selective alteration in patterns of cytokineexpression and recruitment of eosinophil cells similar to that in the OVA-sensitizedanimals.

关键词： airway inflammation antisense oligonucleotides GATA3 transcription factor interferon type Ⅱ interleukin-4 interleukin-5 Tbox expressed in T cells

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Approximate solutions to infinite dimensional LQ problems over infinite time horizon

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Science china Mathematics 2006年第7期49卷 865-876页

作者： PAN Liping, ZHANG Xu & CHEN Qihong School of Mathematical Sciences, Fudan university, Shanghai 200433, china School of Mathematics, sichuan university, chengdu 610064, china department of Applied Mathematics, Shanghai university of Finance & Economics, Shanghai 200433, china School of Mathematical Sciences Fudan University Shanghai 200433 China School of Mathematics Sichuan University Chengdu 610064 China department of Applied Mathematics Shanghai University of Finance & Economics Shanghai 200433 China

This paper is addressed to develop an approximate method to solve a class of infinite dimensional LQ optimal regulator problems over infinite time horizon. Our algorithm is based on a construction of approximate solutions which solve some finite dimensional LQ optimal regulator problems over finite time horizon, and it is shown that these approximate solutions converge strongly to the desired solution in the double limit sense.

关键词： approximate solution LQ problem infinite dimensional infinite time hori-

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Representation of Integers by Ternary Quadratic Forms

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Acta Mathematica Sinica,English Series 2001年第4期17卷 715-720页

作者： De Lang LI Chun Lai ZHAO department of Mathematics, sichuan university, chengdu 610064, P. R. china department of Mathematics, Peking university, Beijing 100871, P. R. china 四川大学数学系四川成都北京大学数学系北京

In this paper we give a formula for the number of representations of some square-free integers by certain ternary quadratic forms and estimate the lower bound of the 2-power appearing in this number.

关键词： Congruent elliptic curve Tate-Shafarevich group Modular form Class number

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Strongly Zero-Dimensional Locales

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Acta Mathematica Sinica,English Series 2002年第1期18卷 47-54页

作者： KOU Hui LUO Mao Kang department of Mathematics. sichuan university, chengdu 610064. P. R. china department of Mathematics Sichuan University Chengdu P. R. China

New kinds of strongly zero-dimensional locales are introduced and characterized, which are different from Johnstone's, and almost all the topological properties for strongly zero-dimensional spaces have the pointloss localic forms. Particularly. the Stone-Cech compactification of a strongly zero-diluensional locale is stongly zero-dimensional.

关键词： Locale Strongly zero-dimensional Compactification

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