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雌三醇对人结肠癌细胞增殖的影响及其机理探讨

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四川医学 2000年第4期21卷 286-288页

作者：黄兴兰吴浩陈斌张正华西医科大学附属第一医院 610041

目的　探讨雌三醇 (E3)对人结肠癌细胞增殖的影响及其机理。方法　采用钙离子荧光指示剂 flour- 3/Am ,流式细胞仪动态检测 E3作用后 ,人结肠癌细胞内游离钙离子 [Ca2 + ]i水平的变化 ,同时用 MTT比色法测定细胞增殖情况。结果　 1E3使... 详细信息

目的　探讨雌三醇 (E3)对人结肠癌细胞增殖的影响及其机理。方法　采用钙离子荧光指示剂 flour- 3/Am ,流式细胞仪动态检测 E3作用后 ,人结肠癌细胞内游离钙离子 [Ca2 + ]i水平的变化 ,同时用 MTT比色法测定细胞增殖情况。结果　 1E3使细胞内 [Ca2 + ]i水平在 1分钟内下降 ,以后维持在一个较低水平 (2 7.88nmol/ L ) ,与静息状态细胞内[Ca2 + ]i水平 (94.4nmol/ L)相比有显著性差异 (P<0 .0 5 )。2 E3对癌细胞有促增殖作用 ,其浓度对数值与细胞增殖率呈显著性正相关 (r=0 .975 ,P<0 .0 5 ) ,其中 1× 10 - 5 mmol/ L浓度的 E3促增殖作用最大。结论　 E3有促人结肠癌细胞增殖作用 ,但不引起细胞内 [Ca2 + ]i水平升高。 E3的促癌细胞增殖作用未见与经钙离子通道引起的非基因的快速调节作用有关 ,推测是基因调节作用的结果。

关键词：雌三醇结肠癌胞内游离钙离子癌细胞增殖

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K28边坡变形破坏机制的离散元模拟

水文地质工程地质 2003年第3期30卷 51-54页

作者：胥良石豫川柴贺军冯学钢李祥海成都理工大学工程地质研究所成都610059 四川省交通厅成都610000

在工程地质勘察的基础上,对K28边坡在天然和饱水状态下的变形进行离散单元法数值模拟,分析了此类缓倾角顺层边坡滑移压致拉裂的变形破坏机制,以及地下水促进边坡变形破坏的作用机理,为边坡的工程治理设计提供合理的建议。

关键词：边坡变形破坏机制离散元模拟工程地质勘察地下水治理措施

C反应蛋白与慢性阻塞性肺疾病的急性加重期关系的探讨

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四川省卫生管理干部学院学报 2004年第1期23卷 2-3页

作者：卿克勤张锐成都市第一人民医院检验科四川成都610016 眉山市第二人民医院四川眉山612160

目的 :探讨C反应蛋白 (CRP)在判断老年慢性阻塞性肺疾病 (COPD)的急性加重期时的作用。方法 :检测慢性阻塞性肺疾病的急性加重期的 4 5名老年患者C反应蛋白、白细胞计数、血沉、体温和临床表现 ,并进行比较 ;同时给予抗生素治疗 ,并比... 详细信息

目的 :探讨C反应蛋白 (CRP)在判断老年慢性阻塞性肺疾病 (COPD)的急性加重期时的作用。方法 :检测慢性阻塞性肺疾病的急性加重期的 4 5名老年患者C反应蛋白、白细胞计数、血沉、体温和临床表现 ,并进行比较 ;同时给予抗生素治疗 ,并比较治疗前后这些指标的变化情况。结果 :C反应蛋白在患者入院时的阳性率为 95 5 6 % ,明显高于其他常规指标 ,并与临床的症状和体征相一致。在治疗后 ,CRP在常规检测指标还未有显著变化时已经明显下降 ,其治疗前后的差异具有统计学意义 (P <0 0 1)。结论 :CRP作为反映细菌性感染的敏感指标 ,对COPD急性加重期的早期发现、反映抗生素疗效方面具有重要意义。

关键词： C反应蛋白 CRP 慢性阻塞性肺疾病急性加重期

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废弃浅埋洞室围岩稳定性及破坏机制分析

四川水力发电 2004年第3期23卷 12-14页

作者：陈光胡卸文西南交通大学土木工程学院四川成都610031

运用快速拉格朗日差分分析程序FLAC研究新疆乌鲁木齐"世纪花苑"三期工程废弃人防地道在拟建高层建筑作用下的稳定性,通过对洞室围岩应力、应变的变化情况、破坏分布状况及发展规律的分析,阐述了相应的破坏机制。

关键词：浅埋洞室围岩稳定性破坏机制 FLAC 高层建筑

Enzymes activity of intestinal grafts after liver small bowel transplantation in rats

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Hepatobiliary & Pancreatic Diseases International 2003年第3期2卷 338-343页

作者： Xiao-Yi Sun Guo Wang Hui-Fen Shi Ming-Fa Wei Guang-Fu Yin Li-Qiang Ru the department of Pediatric Surgery, Tongji hospital, Tongji Medical College department of Neurobiology, School of Basic Medical Sciences Huazhong university of Science and Technology, Wuhan 430030, china department of Pediatric Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. xysuna85@***

OBJECTIVES: To investigate the activity alterations of enzymes in intestine grafts after liver/small bowel transplantation in rats and the relations of these changes to immune rejection of grafts. METHODS: A model of liver/small bowel transplantation (LSBT) was established in closed colony SD and Wistar rats. The activity of enzymes including triphosphatase (ATPase), alkalinophosphatase (AKP), acytelcholinesterase (AchE), oxidesynthase (NOS) and monoamine oxidase (MAO) in bowel grafts was studied histochemically at regular postoperative intervals. RESULTS: The activity of enzymes in the wall of the grafts disappeared eventually in isolated small bowel transplantation (SBT) rats. In contrast, the activity in LSBT rats remained and recovered postoperatively. CONCLUSIONS: The rejection in grafted intestine could be prevented or delayed in LSBT rats. The changes in the activity of enzymes and neurons might be used to detect the rejection and function of the graft.

关键词： small bowel transplantation combined liver/small bowel transplantation enzyme

Transforming growth factor-β1 in invasion and metastasis in colorectal cancer

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World Journal of Gastroenterology 2002年第4期8卷 674-678页

作者： Bin Xiong Hong-Yin Yuan Ming-Bo Hu Feng Zhang Zheng-Zhuan Wei Ling-Ling Gong Guo-Liang Yang department of Oncology,Affiliated Zhongnan hospital of Wuhan university,Wuhan 430071,Hubei Province,china department of Oncology Affiliated Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina. xbxh@***

AIM：To investigate the role of TGFβ1 in invasion and metastasis in colorectal cancer by analysing TGFβ1 correlated wity depth of tumor invasion,stage and ***:Serum TGFβ1levels were determined in50patients with colorectal cancer and 30healthy volunteers using a TGFβ1 enzyme-linked immunosorbent ***β1 expression in primary and lymph node metastatic lesions were detected in 98cases of colorectal cancer by immunohistochemical staining and in situ ***:Serum levels of TGFβ1 in patients with colorectal cancer(40±18μg·L^-1)were significantly higher than those in the healthy control group(19±8μg·L^-1),P<*** levels of serum TGFβ1were found in 60%of patients with colorectal cancer when the mean+2s was used as the upper limit of the normal range(35.1μg·L^-1).Increases in serum TGFβ1 levels were significantly asociated with Dukei's stage(P<0.05),but there was no significant difference between,Duki's stage Bpatients and Dukei's stage *** the cytoplasm of cancer cells,TGFβ1 was immunostained in37.8%(37/98)of colorectal cancer,and this expression was confirmed by in situ hybridization,Among35cases of colorectal cancer with lymph node metastatic lesions,TGFβ1 positive staining was found in18(51.4%)cases of primary tumor,and 25(71.4%)cases with lymph node metastatic lesions,respectively,Of17cases with no staining in the primary lesion.7(41.2%)casesshowed TGFβ1 staining in the metastatic *** TGFβ1 levels and TGFβ1 expression in colorectal cancer tissues were correlated significantly with depth of tumor invasion,stage and metastasis,Patients in stage C-D,T3-T4and with metastasis had significantly higher TGFβ1 levels than patients in stage A-B,T1-T2and without metastasis(P<0.05).CONCLUSION:These results suggest that transforming growth factor-β1 is closely related to the invasion and metastasis of colorectal *** increased the invasive and metastatic potential of tumor by altering a tumor ***β1 ma

关键词：结直肠癌转化生长因子β1 肿瘤侵袭肿瘤转移作用机制

Expression of insulin-like growth factor Ⅱ and its receptor in hepatocellular carcinogenesis

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World Journal of Gastroenterology 2001年第2期7卷 285-288页

作者： Zi Rong Fan Dong Hua Yang Jun Cui Han Rong Qin Chun Chi Huang department of Gastroenterology, Zhujiang hospital. The First Military Medical university, Guangzhou 510282.Guangdong Province. china department of Gastroenterology Zhujiang Hospital The First Military Medical University Guangzhou 510282 Guangdong Province China.

INTRODUCTIONInsulin-like growth factor Ⅱ(IGF-Ⅱ) is a mitogenic peptide of 74 kD and is mostly synthesized in fetal liver tissue .IGF-Ⅱ is believed to play an important role in fetal growth and development and is involved in cellular proliferation and differentiation[1-5]. Recently ,several researchers have reported increased expression of the IGF-Ⅱgene in human hepatocellular carcinoma (HCC) and adjacent non-cancerous liver tissues [6-10].

关键词： liver neoplasms/pathology insulin-like growth factor H/biosynthesis receptors,somatomedin/biosynthesis RNA, messenger/biosynthesis in situ hybridization hepatitis,chronic/pathology

Experimental Study on the Cryopreservation of LLC-PK1 Epithelial Cells with Hypoxic UW Solution

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Journal of Huazhong university of Science and Technology(Medical Sciences) 2007年第4期27卷 426-428页

作者：万赤丹王春友刘涛王宏博杨智勇 department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology

The effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells （LLC-PK1 cells） were cryopreserved in hypoxic UW solution （Ar-UW group） or standard UW solution （UW group） at 4℃ for 48 h. Trypan blue staining and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure in Ar-UW group was significantly declined from 242±6 mmHg to 83±10 mmHg. After cryopreservation at 4℃ for 48 h, LDH leakage rate and Trypan blue-stained rate in Ar-UW group were （11.3±3.4）% and （10.5±4.7）%, respectively, which were significantly lower than in UW group [（49.5±6.9）% and （47.6±9.3）% respectively, both P〈0.01]. It was concluded that lower oxygen partial pressure of UW solution was more beneficial to the cryopreservation of LLC.

关键词： organ preservation solution oxygen partial pressure cryopreservation

Size effect of well-defined nano hydroxyapatite on biological response of osteosarcoma cells

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Size effect of well-defined nano hydroxyapatite on biologica...