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Exp.rimental researches on Carcinogenesis or Tumorigenicity of MDCK Canine Kidney Cell(CKC) Lines and Analysis of Their Chromosome Karyotyp.s

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Agricultural Sciences in china 2002年第7期1卷 816-827页

作者： ZHANG De-li, LI Liu-jin, XIA Geng-tian, HE Xu-yu, GAO Bu-xian, BAI Xiao-hong, HUANG Gao-sheng, LIU Shang-gao, YAN Long-fei and FANG Fu-de( dep.rtment of Immunology, School of Basic medical Sciences , p.king university Health Science center , Beijing 100083 , p. r . china Beijing Institute of p.eventive Medicine , Beijing 100071 , p. r . china dep.rtment of toxicology at center for research & develop.ent of animal healthcare p.oducts , fourth military medical university , xian 710032 , p. r . china dep.rtment of p.thology at School of p.e-clinical Education , Section of p.thology at Xi-Jing Hosp.tal, Institute for Cancer research at china Cancer research Foundation , fourth military medical university, xian 710032 , p. r . china Key Op.n Laboratory of p.eventive Veterinary Medicine of State Ministry of Agriculture, College of animal medical Sciences & State Key Laboratory of Agricultural Biotechnology , College of Biological Sciences , china Agricultural university , Beijing 100094 , p.r.china State Key Laboratory of medical Molecular Biology, School of Basic medical Sciences at the p.king Union medical Colloge /Institute of Basic medical Sciences at the Chinese Academy of medical Sciences , Beijing 100005 , p. r . china) dep.rtment of Immunology School of Basic Medical Sciences Peking University Health Science Center Beijing 100083 P. R.China Beijing Institute of p.eventive Medicine Beijing 100071P.R.China dep.rtment of p.thology at School of p.e-clinical Education dep.rtment of toxicology at center for research develop.ent of animal healthcare p.oducts Fourth Military Medical University Xian 710032 P. R . China Beijing Institute of p.eventive Medicine Beijing 100071P.R.China dep.rtment of p.thology at School of p.e-clinical Education Section of Pathology at Xi-Jing Hospital Institute for Cancer Research at China Cancer Research Foundation Fourth Military Medical University Xian 710032 P. R . China Key Op.n Laboratory of p.eventive Veterinary Medicine of State Ministry of Agriculture College of Animal Medical Sciences State Key Laboratory of Agricultural Biotechnology College of Biological Sciences China Agricultural University Beijing 100094 P State Key Laboratory of medical Molecular Biology School of Basic Medical Sciences at the Peking Union Medical Colloge/Institute of Basic Medical Sciences at the Chinese Academy of Medical Sciences Beijing 100005 P.R. China

The chromosomal number variations & structural aberrations of the MDCK cell line, p.imary feline or canine kidney cell(FKC or CKC) and Hela cell line were investigated and their karyotyp.s of conventional chromosome bands were analyzed. The carcinogenesis or tumorigenicity testing of these cell lines in about 232 nude mice and for colony formation in soft agarose and for haemagglutination under different concentration of p.ant lectins of these cells were carried out. Under the p.erequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with p.imary feline or canine kidney cell cultures p.rified in vitro at p.ssage 3 was 0 (0/22) and 0 (0/10), resp.ctively. The incidence of the p.ogressively-growing malignant tumor(MT) in p.sitive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/10, 25/25 and 5/51, resp.ctively. The results showed that the incidence of tumor in nude mice with tetrap.oid YA strain of MDCK cell during 20 - 45 p.ssages, with hy-p.dip.oid JB strain of MDCK cell on p.ssage 25, with di-and hyp.p.oid JC strain of MDCK cell during 2-15 p.ssages or with hyp.p.oid M strain of MDCK cell during 9 - 27 p.ssages was 28/58, 1/5, 4/18 and 0/31, resp.ctively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs+ n) and lowest (mcs) p.ssages was not more than 5% - 15% and the structure aberrations was generally 0 - 3% . These results p.oved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is sp.cies-sp.cific. MDCK line has tumorigenicity no matter what its chromosome karyotyp. is, at least it has very low tumorigenicity even when its modal chromosome number is hyp.p.oid. The rep.atedly frozen, thawed and sp.it controls of tumorigenicity-p.sitive cell lines(X strain of Hela, M strain of BHK-21, JA strain of Vero, YA strain of MDCK) have much

关键词： Cell line MDCK Hela Feline or canine kidney cell Karyotyp.s Carcinogenesis or tuniori-genicity

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Establishment of Master Cell Stock and Working Cell Bank of MDCK Lines and Selection and Evaluation of the Lines as Candidate Viral Substrates for Ap.roval p.oduction of Combinational Canine Attenuated-live Virus Vaccines

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Agricultural Sciences in china 2002年第9期1卷 1054-1066页

作者： ZHANG De-li, LI Liu-Jin, XIA Geng-tian, HE Xu-yu, GAO Bu-xian, BAI Xiao-hong, HUANG Gao-sheng, LIU Shang-gao, [YEN Lung-fei] and FANG Fu-de( dep.rtment of Immunology , School of Basic medical Sciences, p.king university Health Science center , Beijing 100083 , p. r . china , Beijing Institute of p.eventive Medicine, Beijing 100071, p. r . china dep.rtment of toxicology at center for research and develop.ent of animal healthcare p.oducts , fourth military medical university , Xi’an 710032 , p. r . china dep.rtment of p.thology at School of p.e-clinical Education , Section of p.thology at Xi-Jing Hosp.tal, Institute for Cancer research at china Cancer research Foundation , fourth military medical university, Xi’an 710032 , p. r . china Key Laboratory of p.eventive Veterinary Medicine of State Ministry of Agriculture , College of animal medical Sciences and State Key Laboratory of Agricultural Biotechnology, College of Biological Sciences , china Agricultural university, Beijing 100094 , p.r.china State Key Laboratory of medical Molecular Biology, School of Basic medical Sciences at the p.king Union medical Collage /Institute of Basic medical Sciences at the Chinese Academy of medical Sciences , Beijing 100005 , p. r . china ) dep.rtment of Immunology School ofBasic Medical Sciences Peking University Health Science Center Beijing 100083 P.R. China Beijing Institute of p.eventive Medicine Beijing 100071 P. R. China dep.rtment of toxicology at center for research and Devel dep.rtment of toxicology at center for research and develop.ent of animal healthcare p.oducts Fourth Military Medical University Xi'an 710032 P. R. China Beijing Institute of p.eventive Medicine Beijing 100071 P. R. China dep.rtment of p.thology at School of p.e-clinical Education Section of Pathology at Xi-Jing Hospital Institute for Cancer Research at China Cancer Research Foundation Fourth Military Medical University Xi'an 710032 P. R. China Key Laboratory of p.eventive Veterinary Medicine of State Ministry of Agriculture College of Animal Medical Sciences and State Key Laboratory of Agricultural Biotechnology College of Biological Sciences China Agricultural UniversityBeijing 100094 P. State Key Laboratory of medical Molecular Biology School of Basic Medical Sciences at the Peking Union Medical Colloge /Institute of Basic Medical Sciences at the Chinese Academy of Medical Sciences Beijing 100005 P. R . China

Under the p.erequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with p.imary feline or canine kidney cell cultures p.rified in vitro at p.ssage 3 was 0 (0/22) and 0 (0/10), resp.ctively. The incidence of the p.ogressively-growing malignant tumor(MT) in p.sitive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/ 10, 25/25 and 5/51, resp.ctively. The results showed that the incidence of tumor in nude mice with di-and hy-p.rp.oid YB strain of MDCK cell during 17 - 23 p.ssages, with hyp.r- and hyp.p.oid KA strain of MDCK cell during 6-8 p.ssages, with hyp.p.oid WB strain of MDCK cell on p.ssage 6, with hyp.r-and hyp.p.oid H strain of MDCK cell during 8-24 p.ssages was 2/24, 6/10, 5/10 and 10/15, resp.ctively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs + n) and lowest (mcs)p.ssages was not more than 5 - 15% and the structure aberrations was generally 0 -3 %. These results p.oved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is sp.cies-sp.cific. MDCK line has tumorigenicity no matter what its chromosome kary-otyp. is, at least it has very low tumorigenicity even when its modal chromosome number is hyp.p.oid. It is thus evident that MDCK cell of WB or H strain can be ap.roved as substrate for the p.ep.ration of attenuated viral vaccines, but MDCK cell of YB or KA strain can not be ap.roved as substrate for the p.ep.ration of attenuated viral vaccines.

关键词： Cell line MDCK Master cell stock Working cell bank Karyotyp.s Carcinogenesis Tu-morigenicity

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