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Evaluation of steroidogenic capacity after follicle stimulating hormone stimulation in bovine granulosa cells of Revalor 200~ implanted heifers

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Journal of Animal Science and Biotechnology 2014年第3期5卷 280-285页

作者： ANDrea D Stapp Craig A Gifford dennis m hallford Jennifer A Hernandez Gifford Department of Animal Science Oklahoma State University Department of Animal and Range Science New Mexico State University Department of Animal Science 114B Animal Science BuildingOklahoma State University

Background： Heifers not used as breeding stock are often implanted with steroids to increase growth efficiency thereby altering hormone profiles and potentially changing the environment in which ovarian follicles develop. Because bovine granulosa cell culture is a commonly used technique and often bovine ovaries are collected from abattoirs with no record of implant status, the objective of this study was to determine if the presence of an implant during bovine granulosa cell development impacts follicle stimulating hormone-regulated steroidogenic enzyme expression. Paired ovaries were collected from 16 feedlot heifers subjected to 1 of 3 treatments： non-implanted （n = 5）, Revalor 200 for 28 d （n = 5）, or Revalor 200 for 84 d （n = 6）. Small follicle （1 to 5 mm） granulosa cells were isolated from each pair and incubated with phosphate buffered saline （n = 16） or 100 ng/mL follicle stimulating hormone （n = 16） for 24 h. Results： Granulosa cells of implanted heifers treated with follicle stimulating hormone produced medium concentrations of progesterone similar （P = 0.22） to non-implanted heifers, while medium estradiol concentrations were increased （P 〈 0.10） at 28 and 84 d compared to non-implanted heifers indicating efficacy of treatment. Additionally, real-time PCR analysis in response to follicle stimulating hormone treatment demonstrated a decrease in steroidogenic acute regulatory protein （P = 0.05） mRNA expression in heifers implanted for 84 d and an increase in P450 side chain cleavage mRNA in granulosa cells of heifers implanted for 28 （P 〈 0.10） or 84 d （P 〈 0.05） compared to non-implanted females. However, no difference in expression of 3-beta-hydroxysteroid dehydrogenase （P= 0.57） and aromatase （P = 0.23） were demonstrated in implanted or non-implanted heifers. Conclusions： These results indicate follicles which develop in the presence of high concentrations of androgenic and estrogenic steroids via an implant tend to demonstrate an altered capacity to res

关键词： Bovine Follicle stimulating hormone Granulosa cells Implant Steroidogenesis

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Ion emission from warm dense matter produced by irradiation with a soft x-ray free-electron laser

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matter and Radiation at Extremes 2024年第1期9卷 71-84页

作者： Josef Krása Tomás Burian Vera Hájková Jaromír Chalupský Šimon Jelínek Katerina Frantálová michal Krupka Zuzana Kuglerová Sushil Kumar Singh Vojtech Vozda Ludek Vysín michalŠmíd Pablo Perez-martin marion Kühlman Juan Pintor Jakub Cikhardt matthias Dreimann dennis Eckermann Felix Rosenthal Sam m.Vinko Alessandro Forte Thomas Gawne Thomas Campbell Shenyuan Ren YuanFeng Shi Trevor Hutchinson Oliver Humphries Thomas Preston mikako makita motoaki Nakatsutsumi Xiayun Pan Alexander Köhler marion Harmand Sven Toleikis Katerina Falk Libor Juha Department of Radiation and Chemical Physics Institute of PhysicsCzech Academy of SciencesNa Slovance 218221 Prague 8Czech Republic Laser Plasma Department Institute of Plasma PhysicsCzech Academy of SciencesZa Slovankou 318200 Prague 8Czech Republic Department of Surface and Plasma Science Faculty of Mathematics and PhysicsCharles University in PragueV Holesovi ckách 218000 Prague 8Czech Republic Department of Physical Electronics Faculty of Nuclear Science and Engineering PhysicsCzech Technical University in PragueV Holesovi ckách 218000 Prague 8Czech Republic Helmholtz-Zentrum Dresden-Rossendorf Bautzner Landstraße 40001328 DresdenGermany DESY Photon Science Notkestraße 85D-22607 HamburgGermany Institut de minéralogie de Physique des Matériaux et de CosmochimieUMR 7590–UPMC/CNRS/IRD/MNHNSorbonne Université4 place Jussieu75005 ParisFrance Department of Physics Faculty of Electrical EngineeringCzech Technical University in PragueTechnická216627 Prague 6Czech Republic Center for Soft Nanoscience University of MünsterBusso-Peus-Straße 10D-48149 MünsterGermany Department of Physics Clarendon LaboratoryUniversity of OxfordParks RoadOxford OX13PUUnited Kingdom Central Laser Facility STFC Rutherford Appleton LaboratoryDidcot OX110QXUnited Kingdom Lawrence Livermore National Laboratory 7000 East AvenueLivermoreCalifornia 94550USA European XFEL GmbH Holzkoppel 4D-22869 SchenefeldGermany Technische Universität Dresden 01062 DresdenGermany

We report on an experiment performed at the FLASH2 free-electron laser(FEL)aimed at producing warm dense matter via soft x-ray isochoric *** the experiment,we focus on study of the ions emitted during the soft x-ray ablation process using time-of-flight electron multipliers and a shifted maxwell–Boltzmann velocity distribution *** find that most emitted ions are thermal,but that some impurities chemisorbed on the target surface,such as protons,are accelerated by the electrostatic field created in the plasma by escaped *** morphology of the complex crater structure indicates the presence of several ion groups with varying *** find that the ion sound velocity is controlled by the ion temperature and show how the ion yield depends on the FEL radiation attenuation length in different materials.

关键词： electrostatic emitted chemi

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Evaluation of a Wireless Solar Powered Personal Weather Station

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Agricultural Sciences 2024年第1期15卷 36-53页

作者： Robert J. Lascano Timothy S. Goebel dennis C. Gitz III John E. Stout Cropping Systems Research Laboratory Wind Erosion and Water Conservation Research Unit USDA-ARS Lubbock TX USA

We are evaluating dryland cotton production in martin County, Texas, measuring cotton lint yield per unit of rainfall. Our goal is to collect rainfall data per 250 - 400 ha. Upon selection of a rainfall gauge, we realized that the cost of using, for example, a tipping bucket-type rain gauge would be too expensive and thus searched for an alternative method. We selected an all-in-one commercially available weather station;hereafter, referred to as a Personal Weather Station (PWS) that is both wireless and solar powered. Our objective was to evaluate average measurements of rainfall obtained with the PWS and to compare these to measurements obtained with an automatic weather station (AWS). For this purpose, we installed four PWS deployed within 20 m of the Plant Stress and Water Conservation meteorological Tower that was used as our AWS, located at USDA-ARS Cropping Systems Research Laboratory, Lubbock, TX. In addition, we measured and compared hourly average values of short-wave irradiance (Rg), air temperature (Tair) and relative humidity (RH), and wind speed (WS), and calculated values of dewpoint temperature (Tdew). This comparison was done over a 242-day period (1 October 2022-31 may 2023) and results indicated that there was no statistical difference in measurements of rainfall between the PWS and AWS. Hourly average values of Rg measured with the PWS and AWS agreed on clear days, but PWS measurements were higher on cloudy days. There was no statistical difference between PWS and AWS hourly average measurements of Tair, RH, and calculated Tdew. Hourly average measurements of Rg and WS were more variable. We concluded that the PWS we selected will provide adequate values of rainfall and other weather variables to meet our goal of evaluating dryland cotton lint yield per unit rainfall.

关键词： Automation Sensors Citizen Weather Station mesonet Rainfall Weather Variables

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movie therapy for children with amblyopia:restoring binocular vision with brain plasticity

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Science China(Life Sciences) 2022年第3期65卷 654-656页

作者： Bernhard A.Sabel dennis m.Levi Institute of medical Psychology Medical FacultyOtto-v.-Guericke University of MagdeburgMagdeburg 39120Germany Herbert Wertheim School of Optometry&Vision Science and Helen Wills Neuroscience Institute University of CaliforniaBerkeley CA 94720USA

Amblyopia is a common neurodevelopmental disorder that occurs when the two eyes do not send well matched visual signals to the brain early in *** mismatch of the information coming from both eyes can have different causes:it may be due to differences in image clarity or contrast(called"anisometropia")because the two eyes require different corrective lens powers.

关键词： movie matched visual

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Performance evaluation of a water erosion tracer using plot-scale experiments and process-based modeling

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International Soil and Water Conservation Research 2023年第4期11卷 622-632页

作者： João m.Villela Jamil A.A.Anache Alex m.Watanabe dennis C.Flanagan Edson C.Wendland Silvio Crestana EmBRAPA-Brazilian Agricultural Research Corporation LNNA-National Laboratory of Nanotechnology for AgrobusinessSt.XV de NovembroSão CarlosSP13560-970Brazil University of São Paulo(USP) São Carlos School of Engineering.Graduate Program in Environmental Engineering Sciences(PPG-SEA)Av.Trabalhador São-CarlenseCxP.359São CarlosSP13566590Brazil Department of Hydraulics and Sanitation São Carlos School of Engineering(EESC)University of São Paulo(USP)CxP.359São CarlosSP13566-590Brazil Federal University of mato Grosso Do Sul CxP.549Campo Grande.MS.79070-900Brazil USDA-Agricultural Research Service National Soil Erosion Research Laboratory275 S.Russell StWest LafayetteIN47907USA

Socioeconomic and environmental losses caused by water erosion have highlighted the importance of quantifying and understanding the dynamics of soil redistribution in the landscape to develop effective soil management *** methods are applied to estimate erosion/deposition rates and identify sources of sediments,among them,the one that uses rare earth elements(REE)as a tracer stands ***,an alternative not yet explored that can benefit the accuracy of the estimates provided by the method is using a tracer containing a chemical signature composed of more than one *** present study aimed to evaluate the performance of a new water erosion tracer based on montmoril-lonite labeled with rare earth elements(La40-mmT).The innovative aspects of this La40-mmT tracer include its highly stable multi-chemical signature(Nd^(3+),La^(3+),and Pr^(3+)),which enhances tracer detection in the environment,and its low production cost due to the use of an industrial residue in the synthesis *** tracer was evaluated for a typical soil of the Cerrado biome,using a natural rainfall field-scale plot-NRFP(5 m × 20 m)and a physical predictive erosion model(WEPP).The results showed that the La40-mmT tracer could be used to estimate erosion/deposition rates,with agreement between the values observed with the tracer and the WEPP ***,this study confirmed the great potential of La40-mmT as a tool to identify patterns of soil redistribution at the field scale and aid in the validation of erosion models.

关键词： Soil erosion Tracer Rare earth elements Deposition Sediment source WEPP model

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Optimal investment analysis for heat pumps and nuclear heat in decarbonised Helsinki metropolitan district heating system

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Energy Storage and Saving 2022年第2期1卷 80-92页

作者： Esa Pursiheimo Tomi JLindroos dennis Sundell miika Rämä Ville Tulkki Smart Energy and Built Environment VTT Technical Research Centre of FinlanFI-02044 VTTFinland Nuclear Energy VTT Technical Research Centre of FinlandFI-02044 VTTFinland

Decarbonisation of district heating and cooling(DHC)system in Helsinki metropolitan area requires investments in new energy technologies and approaches to replace fossil fuel fired district heating(DH)***-ment paths involving(a)DH heat pumps(HPs)from low quality heat sources and(b)small modular nuclear reactors(SmR)are compared by utilising investment analysis based on optimisation model depicting the as-sumed 2030 *** scenarios,with varying assumptions concerning existing DHC system,investment costs and electricity prices,are analysed in terms of new capacity and total annualised *** results indicate that the SmR option is more cost-efficient than the HP option with 4-8€/mWh difference in operation costs including annualised *** fired boiler investments,enabled in both options,are preferred to HP investments in most *** cost-efficiency of HP investments is sensitive to investment cost,whereas SmR investments are relatively stable to investment cost *** electricity market prices affect cost-efficiency of large-scale HPs,and investments in SmR cogeneration units take place only with high electricity prices.

关键词： District heating Decarbonisation Heat pumps Small modular reactors Optimisation model

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modeling and Validation of Base Pressure for Aerodynamic Vehicles Based on machine Learning models

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Computer modeling in Engineering & Sciences 2023年第12期137卷 2331-2352页

作者： Jaimon dennis Quadros Sher Afghan Khan Abdul Aabid muneer Baig Senior Lecturer of mechanical Engineering University of BoltonRAK Academic CenterRas Al Khaimah16038UAE Department of mechanical and Aerospace Engineering Faculty of EngineeringInternational Islamic University MalaysiaSelangor53100Malaysia Department of Engineering management College of EngineeringPrince Sultan UniversityRiyadh11586Saudi Arabia

The application of abruptly enlarged flows to adjust the drag of aerodynamic vehicles using machine learning models has not been investigated *** process variables(mach number(m),nozzle pressure ratio(η),area ratio(α),and length to diameter ratio(γ))were numerically explored to address several aspects of this process,namely base pressure(β)and base pressure with cavity(βcav).In this work,the optimal base pressure is determined using the PCA-BAS-ENN based algorithm to modify the base pressure presetting accuracy,thereby regulating the base drag required for smooth flow of aerodynamic *** on the identical dataset,the GA-BP and PSO-BP algorithms are also compared to *** data for training and testing the algorithmswas derived using the regression equation developed using the Box-Behnken Design(BBD).The results show that the PCA-BAS-ENN model delivered highly accurate predictions when compared to the other two *** a result,the advantages of these results are two-fold,providing:(i)a detailed examination of the efficiency of different neural network algorithms in dealing with a genuine aerodynamic problem,and(ii)helpful insights for regulating process variables to improve technological,operational,and financial factors,simultaneously.

关键词： High speed flow mach number machine learning PCA-BAS-ENN algorithm

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Laser Spectroscopy's Roaring Nineties:Recollections from the First Decade at UGA

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发光学报 2008年第4期29卷 622-626页

作者： William m dennis Department of Physics and Astronomy University of Georgia Athens GA 30602

The first decade at the University of Georgia(UGA) was an active and exciting time for Bill Yen,his students,post-docs and *** this paper,I highlight several papers that I hope capture this activity and provide a glim... 详细信息

关键词：激光光谱学科学研究工作美国光学研究

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Part II: Explaining Black Hole Growth due to Universal Expansion: Probabilistic Spacetime versus GEODEs

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Journal of High Energy Physics, Gravitation and Cosmology 2023年第2期9卷 530-541页

作者： dennis m. Doren James Harasymiw Independent Researcher Wisconsin USA

Recent research indicates that black holes can grow based on the expansion of the universe and not just through accretion and mergers. Two different models independently predicted that finding. One model, describing the relevant massive star remnants as “generic objects of dark energy”, rejects the traditional view of black holes while hypothesizing that dark energy causes the cosmologically coupled growth of these objects. The other model, based on the probabilistic spacetime theory, indicates the growth of black holes is based on the same spacetime mechanism underlying all universal expansion, and does so while leaving the traditional black hole conceptualization essentially intact. The fact these two models predicted this observational finding but did so from different perspectives suggests more can be learned by further study of their differences. This paper explores similarities and differences in the two models’ explanations for massive star remnants’ growth, concluding with suggestions for research testing their relative veracity. An exploration of the relative utility and parsimony of the two models is also described.

关键词： Probabilistic Spacetime GEODE Cosmological Coupling Universal Expansion Black Holes

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Resolving the Information Paradox with Probabilistic Spacetime

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Journal of High Energy Physics, Gravitation and Cosmology 2023年第1期9卷 83-99页

作者： dennis m. Doren James Harasymiw Wisconsin USA

It has been 50 years since Hawking described the black hole (BH) information paradox. The combination of BH radiation and subsequent BH evaporation was found to take trapped information into oblivion contrary to the law of conservation of quantum information. Numerous attempts have been made since to resolve this paradox. A brief review herein documents how all these attempts have significant shortcomings, meaning the paradox is still unresolved. A relatively new cosmological theory offers a resolution despite not being developed for that purpose. The theory, entitled the probabilistic spacetime theory (PST), starts with an alteration in one basic assumption compared to all current cosmological theories. Spacetime, instead of being seen as a void or container of other entities, is viewed as the most fundamental entity in the universe, composed of energy fragments, and (in keeping with the conservation principle) impermeable to destruction. The potential contribution of the PST in resolving the information paradox is delineated, with the finding that the single change in the conceptualization of spacetime results in the disappearance of the paradox and not information.

关键词： Information Paradox Hawking Radiation Probabilistic Spacetime Black Holes Energy Fragment

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