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Optimal concentration and time window for proliferation and differentiation of neural stem cells from embryonic cerebral cortex: 5% oxygen preconditioning for 72 hours

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Neural Regeneration Research 2015年第9期10卷 1516-1522页

作者： Li-li Yuan Ying-jun Guan deng-dian ma Hong-mei Du Department of Histology and Embryology Academy of Basic Medicine Jining Medical University Department of Histology and Embryology Academy of Basic Medicine Weifang Medical University Department of Otorhinolaryngology Affiliated Hospital of Jining Medical University

Hypoxia promotes proliferation and differentiation of neural stem cells from embryonic day 12 rat brain tissue, but the concentration and time of hypoxic preconditioning are controversial. To address this, we cultured neural stem cells isolated from embryonic day 14 rat cerebral cortex in 5% and 10% oxygen in vitro. MTT assay, neurosphere number, and immunofluorescent staining found that 5% or 10% oxygen preconditioning for 72 hours improved neural stem cell viability and proliferation. With prolonged hypoxic duration （120 hours）, the proportion of apoptotic cells increased. Thus, 5% oxygen preconditioning for 72 hours promotes neural stem cell prolif- eration and neuronal differentiation. Our findings indicate that the optimal concentration and duration of hypoxic preconditioning for promoting proliferation and differentiation of neural stem cells from the cerebral cortex are 5% oxygen for 72 hours.

关键词： nerve regeneration brain injury neural stem cells low oxygen cerebral cortex apoptosis differentiation microtubule-associated protein 2 glial fibrillary acidic protein caspase-3 neuralregeneration

Digital generation of super-Gaussian perfect vortex beams via wavefront shaping with globally adaptive feedback

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High Power Laser Science and Engineering 2024年第1期12卷 42-51页

作者： Rui ma Ke Hai Luo Jing Song He Wei Li Zhang dian Yuan Fan Anderson S.L.Gomes Jun Liu International Collaborative Laboratory of 2D materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale OptoelectronicsShenzhen UniversityShenzhenChina Institute for Advanced Study Shenzhen UniversityShenzhenChina Fiber Optics Research Centre School of Information and Communication EngineeringUniversity of Electronic Science and Technology of ChinaChengduChina Departamento de Física Universidade Federal de PernambucoRecife-PEBrazil

High-intensity vortex beams with tunable topological charges and low coherence are highly demanded in applications such as inertial confinement fusion(ICF) and optical communication. However, traditional optical vortices featuring nonuniform intensity distributions are dramatically restricted in application scenarios that require a high-intensity vortex beam owing to their ineffective amplification resulting from the intensity-dependent nonlinear effect. Here, a low-coherence perfect vortex beam(PVB) with a topological charge as high as 140 is realized based on the super-pixel wavefront-shaping technique. More importantly, a globally adaptive feedback algorithm(GAFA) is proposed to efficiently suppress the original intensity fluctuation and achieve a flat-top PVB with dramatically reduced beam speckle contrast. The GAFA-based flat-top PVB generation method can pave the way for high-intensity vortex beam generation,which is crucial for potential applications in ICF, laser processing, optical communication and optical trapping.

关键词： digital micromirror device flat-top beam orbital angular momentum perfect vortex beam random fiber laser

Deleterious variants in RNF111 impair female fertility and induce premature ovarian insufficiency in humans and mice

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Science China(Life Sciences) 2024年第7期67卷 1325-1337页

作者： Chengcheng Song Yingying Qin Yan Li Bingyi Yang Ting Guo Wenqing ma dian Xu Keyan Xu Fangfang Fu Li Jin Yanhua Wu Shuyan Tang Xiaojun Chen Feng Zhang Obstetrics and Gynecology Hospital State Key Laboratory of Genetic EngineeringInstitute of Medical Genetics and GenomicsFudan UniversityShanghai 200011China Human Phenome Institute Zhangjiang Fudan International Innovation CenterFudan UniversityShanghai 201203China Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250021China State Key Laboratory of Reproductive Medicine and Offspring Health Key Laboratory of Reproductive Endocrinology of Ministry of EducationNational Research Center for Assisted Reproductive Technology and Reproductive GeneticsShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthShandong UniversityJinan 250021China Department of Obstetrics and Gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China National Demonstration Center for Experimental Biology Education School of Life SciencesFudan UniversityShanghai 200433China Department of Gynecology the Tenth People’s Hospital of Tongji UniversityShanghai 200072China Soong Ching Ling Institute of maternity and Child Health International Peace Maternity and Child Health Hospital of China Welfare InstituteShanghai 200030China

Premature ovarian insufficiency(POI)is a heterogeneous female disorder characterized by the loss of ovarian function before the age of *** represents a significant detriment to female ***,the known POI-causative genes currently account for only a fraction of *** elucidate the genetic factors underlying POI,we conducted whole-exome sequencing on a family with three fertile POI patients and identified a deleterious missense variant in *** a subsequent replication study involving 1,030 POI patients,this variant was not only confirmed but also accompanied by the discovery of three additional predicted deleterious RNF111 *** variants collectively account for eight cases,representing 0.78%of the study cohort.A further study involving 500 patients with diminished ovarian reserve also identified two additional RNF111 ***,RNF111 encodes an E3 ubiquitin ligase with a regulatory role in the TGF-β/BMP signaling *** analysis revealed that RNF111/RNF111 is predominantly expressed in the oocytes of mice,monkeys,and *** further investigate the functional implications of RNF111 variants,we generated two mouse models:one with a heterozygous missense mutation(Rnf111+/M)and another with a heterozygous null mutation(Rnf111^(+/-)).Both mouse models exhibited impaired female fertility,characterized by reduced litter sizes and small ovarian ***,RNA-seq and quantitative proteomics analysis unveiled that Rnf111 haploinsufficiency led to dysregulation in female gonad development and negative regulation of the BMP signaling pathway within mouse *** conclusion,our findings strongly suggest that monoallelic deleterious variants in RNF111 can impair female fertility and induce POI in both humans and mice.

关键词： POI RNF111 female fertility ovarian reserve

In situ regulating intimately connected heterostructure by decomposition of solid solution oxides toward high-efficient water oxidation

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Rare Metals 2024年第4期43卷 1557-1569页

作者： Shao-Fei Zhang Xiao-Lu Yin Jin Wang Jian-Li Kang Tian-Tian Li Jin-Feng Sun Yong-Qiang Meng Jian-Jiang Wang dian-Long Wang Kai-Qiang Qin Hebei Key Laboratory of Flexible Functional materials/Hebei Key Laboratory of material Near-Net Forming Technology School of Materials Science and EngineeringHebei University of Science and TechnologyShijiazhuang050018China Key Laboratory of Advanced Ceramics and machining Technology/Ministry of Education School of Materials Science and EngineeringTianjin UniversityTianjin300072China Electrochemical Engine Center Department of Mechanical EngineeringPennsylvania State UniversityUniversity ParkPA16802USA

Heterogeneous interfaces produced by interdomain interactions on a nanoscale performs a crucial role in boosting the properties of an electrocatalyst toward oxygen evolution reaction(OER)***,a series of dual-phase electrodes with intimately connected heterointerfaces are prepared by in situ decomposing solid solution oxide of Ni_(x)Co_(y)Fe_(100-x-y)O,which grew on Ni foam massively via an ultrafast combustion ***,with high-reaction kinetics caused by the reduction treatment at 450℃,the less electronegative Fe and Co are more oxyphilic than Ni,which facilitated their co-exsolution and formation of CoFe_2O_4/NiO oxide with enriched oxygen *** from the nanoporous framework,heterojunction structure,and oxygen defects,the self-supporting electrodes present rapid charge/mass transmission and provide abundant active sites for *** optimized sample(R-SNCF4.5)shows low overpotentials of 226 and 324 mV at 10 and100 ma·cm^(-2),a small Tafel slope(46.7 mV·dec^(-1)),and excellent *** assembled R-SNCF4.5//Pt/C/NF electrolyzer demonstrates continuous electrolysis over 50 h at a current density of 10 ma·cm^(-2),under 1.51 *** functional theory(DFT)calculations verify that the strong electronic modulation plays a critical part in the CoFe_2O_4/NiO hybrid by lowering the energy barriers for the ratedetermining steps,and Fe sites are the most active OER sites.

关键词： Rapid combustion Decomposition of solid solution oxide Dual-phase heterostructure Oxygen evolution reaction Theoretical calculation

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NiMo基非晶合金电催化析氢反应的进展

Transactions of Nonferrous Metals Society of China 2024年第1期34卷 26-49页

作者：张思璇黄金昭丁殿金汤军邓小龙济南大学物理科学与技术学院济南250022 安徽工业大学数学与物理学院马鞍山243032

电催化水分解产生绿色氢气是发展可持续能源体系的重要途径之一。由过渡金属Ni、Mo组成的具有高活性、低成本的非晶态合金被广泛认为是一种有效的制氢催化剂。重点关注以镍钼(NiMo)基非晶合金为代表的催化剂,总结非晶态合金的制备方法... 详细信息

电催化水分解产生绿色氢气是发展可持续能源体系的重要途径之一。由过渡金属Ni、Mo组成的具有高活性、低成本的非晶态合金被广泛认为是一种有效的制氢催化剂。重点关注以镍钼(NiMo)基非晶合金为代表的催化剂,总结非晶态合金的制备方法及其在催化领域的应用,分别叙述NiMo合金、NiMo基中熵合金和NiMo基高熵合金在电催化析氢反应(HER)中的应用。结果表明,在合理的功能导向设计下,通过对关键科学及技术问题的解决,NiMo基非晶态合金催化剂能够表现出良好的析氢性能,最后提出在制备技术、组分调控、纳米化、电子结构以及活化机理5个方面还有待解决的问题。这对于催化剂的设计、制备、构效关系及催化机理的研究具有一定的参考价值。

关键词： NiMo合金非晶合金水电解析氢反应

基于MSTP协议和5G双域技术的校园融合组网方法研究

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信息技术与信息化 2024年第4期 37-44页

作者：马峥李行张典中国地质大学(武汉) 湖北武汉430074

在高校多校区网络建设中,当前组网技术存在功能单一、适用范围有限、安全性低、维护成本较高等问题,无法满足数字化转型对网络高速互联互通和安全稳定的要求。首先梳理了校园常用组网技术,并研究了融合组网技术原理。然后在此基础上,提... 详细信息

在高校多校区网络建设中,当前组网技术存在功能单一、适用范围有限、安全性低、维护成本较高等问题,无法满足数字化转型对网络高速互联互通和安全稳定的要求。首先梳理了校园常用组网技术,并研究了融合组网技术原理。然后在此基础上,提出了一种校园融合组网方法,利用MSTP协议和5G双域网技术实现适用于不同场景的传输技术整合,整体实现链路保障、接入鉴权、安全隔离、分析预测功能。最后在学校真实校园网环境中完成融合组网部署测试,实验结果达到预期成效。

关键词：校园网 MSTP 5G双域融合组网流量预测

基于分布式深度强化学习的六足机器人步态学习与控制

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组合机床与自动化加工技术 2024年第8期 85-89页

作者：李伦向国菲马丛俊佃松宜四川大学电气工程学院成都610065

针对六足机器人系统结构与决策的复杂导致步态规划与控制困难的问题,提出了一种基于分布式强化学习的六足机器人步态学习与控制框架。该框架采用大规模并行学习的方式获取训练数据,通过强化学习的方法对网络进行数据驱动训练,得到最优... 详细信息

针对六足机器人系统结构与决策的复杂导致步态规划与控制困难的问题,提出了一种基于分布式强化学习的六足机器人步态学习与控制框架。该框架采用大规模并行学习的方式获取训练数据,通过强化学习的方法对网络进行数据驱动训练,得到最优控制策略,并在IsaacGym平台下构建仿真环境评估和验证六足机器人步态学习与控制的性能。结果表明,训练的六足机器人步态在奖励值、速度跟踪和稳定性都有良好的表现,验证了所提方法的有效性。

关键词：六足机器人分布式深度强化学习步态学习

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人工岛与多工程影响下的潮流动力响应特征

科学技术与工程 2024年第5期24卷 1837-1847页

作者：纪超姜奇马殿光吕彪周俊伟交通运输部天津水运工程科学研究所港口水工建筑技术国家工程研究中心天津300456 中交第一航务工程勘察设计院有限公司天津300222

人工岛常与连岛桥、海岸防护等工程构成复杂的人工岛工程系统,科学评估人工岛与多工程对周围海域潮流动力的影响,对海洋空间资源的开发与利用具有重要的指导意义。以海南三亚新机场工程为对象,建立了工程海域潮流数学模型,采用实测的潮... 详细信息

人工岛常与连岛桥、海岸防护等工程构成复杂的人工岛工程系统,科学评估人工岛与多工程对周围海域潮流动力的影响,对海洋空间资源的开发与利用具有重要的指导意义。以海南三亚新机场工程为对象,建立了工程海域潮流数学模型,采用实测的潮位、流速、流向数据对模型进行了验证,模拟研究了人工岛、离岸潜堤和连岛桥等多工程作用下的潮流场响应特性。结果表明:工程海域的主体潮流特征为往复流,人工岛建设后其周围产生绕流,人工岛东北偏北和西南偏南两侧流速增大,西北偏西和东南偏东两侧形成缓流区,流速显著减小;弧形离岸潜堤修建后,其南北两侧由于绕流作用流速增大,弧内与东西两侧因阻水影响流速减小;连岛桥建设对潮流场的影响相对较小,主要表现为连岛桥背流侧的流速总体减小,而在人工岛与离岸潜堤的部分掩护区域,连岛桥则会导致流速有所增加。总体而言,在人工岛、离岸潜堤和连岛桥的共同作用下,工程海域潮流动力的时空变化将更为复杂,工程对不同位置处水流运动的影响规律存在明显的差异。

关键词：人工岛离岸潜堤连岛桥潮流数值模拟

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基于改进势场法的智能车超车动态路径规划

科学技术与工程 2024年第20期24卷 8746-8753页

作者：张喜清马志光陈殿明李进太原科技大学车辆与交通工程学院太原030000 太原科技大学机械工程学院太原030000

为了改进人工势场法(artificial potential field,APF)在智能车高速超车状态下规划出的轨迹规范性差、安全性得不到保障、以及路径不平滑的问题。设计用于指引智能车换道的换道引力势场,以保证换道轨迹的规范性;构建异向分布结构的障碍... 详细信息

为了改进人工势场法(artificial potential field,APF)在智能车高速超车状态下规划出的轨迹规范性差、安全性得不到保障、以及路径不平滑的问题。设计用于指引智能车换道的换道引力势场,以保证换道轨迹的规范性;构建异向分布结构的障碍车斥力势场提高换道的安全性;并建立带有转向缺口的道路中心线斥力势场和设计平滑代价函数优化规划出的轨迹点,使规划出的路径更加平滑。基于线性二次型调节器(linear quadratic regulator,LQR)设计路径跟踪控制器跟踪规划出的路径。通过Simulink与Carsim联合仿真,结果表明:所改进的APF算法能规划出更加合理的路径,设计的LQR路径跟踪控制器跟踪误差趋近于零,能够较好地控制车辆完成超车换道。

关键词：改进的人工势场法(APF) 高速超车换道局部路径规划线性二次型调节器(LQR)控制器路径跟踪