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第十一届全国免疫学学术大会

作者： Fang Xiang deng guo-min Key Laboratory of Antibody Techniques of ministry of Health Nanjing Medical University

Objective:Systemic lupus erythematosus(SLE)is an autoimmune disease characterized by high auto-antibody levels and multi-organ tissue damage,including kidney and *** disorders are frequent in patients with SLE,yet the... 详细信息

关键词： Systemic lupus erythematosus(SLE) liver apoptosis immune complexes(ICs)

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Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alphasynuclein

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Neural Regeneration Research 2012年第2期7卷 85-90页

作者： Tao Chen Xiaoping Liao guoqiang Wen Yidong deng min guo Zhigang Long Feng Ouyang Department of Neurology Hainan Provincial People＇s Hospital Haikou 570311 Hainan Province China

The specific and effective a-synuclein RNA interference （RNAi） plasmids, and the a-synuclein-pEGFP recombinant plasmids were co-transfected into human embryonic kidney 293 （HEK293） cells using the lipofectamine method. Using an inverted fluorescence microscope, a-synuclein proteins were observed to aggregate in the cytoplasm and nucleus. Wild-type a-synuclein proteins co-localized with mitochondria. Hematoxylin-eosin staining revealed round eosinophilic bodies （Lewy body-like inclusions） in the cytoplasm of some cells transfected with a-synuclein-pEGFP plasmid. However, the formation of Lewy body-like inclusions was not observed following transfection with the RNAi pSYN-1 plasmid. RNAi blocked Lewy body-like inclusions in the cytoplasm of HEK293 cells induced by wild-type a-synuclein overexpression, but RNAi did not affect the subcellular localization of wild-type a-synuclein in mitochondria.

关键词： RNA interference a-synuclein gene subcellular localization inclusion Parkinson'sdisease neural regeneration

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The role of Syk in the pathogenesis of bacterial DNA-induced arthritis

The role of Syk in the pathogenesis of bacterial DNA-induced...

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第十一届全国免疫学学术大会

作者： Liu Huicheng Qiao Wei deng guo-min Key Lab of Antibody Techniques of ministry of Health Nanjing Medical University

Objective: Bacterial DNA has immunostimulatory effect and can induce arthritis;inhibitor of Syk has therapeutic effect in patients with rheumatoid arthritis. In this study, we investigated the role of Syk in the pathogenesis of bacterial DNAinduced ***: We evaluated the role of Syk inhibitor in prevention and treatment of bacterial DNA-induced arthritis animal model and investigated the mechanism that Syk regulated bacterial DNA-induced arthritis. Results: We found that bacterial DNA induced arthritis;inhibitor of Syk R788 prevented and treated arthritis induced by bacterial *** of monocytes/macrophages significantly reduced incidence and severity of arthritis induced by bacterial DNA;Incidence and severity of arthritis induced by bacterial DNA were markedly decreased in TNF deficient mice compared to wild mice. Bacterial DNA stimulated monocytes/macrophages to assembly of lipid rafts that recruit Syk and Toll-like receptor 9(TLR9). Depletion of lipid rafts reduced severity of arthritis induced by bacterial ***: Syk exerts important role in the pathogenesis of bacterial DNA-induced arthritis;Syk regulates signaling pathway of TLR through lipid rafts.

关键词： Bacterial DNA Syk TLR9

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智能时代教育研究的认识论变革与方法论转变

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现代教育技术 2020年第5期30卷 26-32页

作者：邓国民贵阳学院教育科学学院贵州贵阳550005

文章对教育领域的"三个世界"及其智能虚拟映射进行了认识切割,在此基础上提出了三个新的智能虚拟世界:代表数字化教育事实和存在的世界一’、代表人工智能教育认识的世界二’、代表人工智能教育思想和知识库的世界三’。文章指出,这三... 详细信息

文章对教育领域的"三个世界"及其智能虚拟映射进行了认识切割,在此基础上提出了三个新的智能虚拟世界:代表数字化教育事实和存在的世界一’、代表人工智能教育认识的世界二’、代表人工智能教育思想和知识库的世界三’。文章指出,这三个新的智能虚拟世界是教育领域的"三个世界"在人工智能环境下的智能虚拟映射,扩展了传统教育研究的本体论假设,进而导致认识论的全方位变革。此外,文章还从认识主体、研究对象、研究范式、推理思维、知识表征等五个方面的变化,阐明了教育研究的认识论变革所引发的方法论转变。文章的研究有助于理解智能时代教育的过程与本质,并为教育人工智能系统的设计、开发与应用提供理论依据。

关键词：教育人工智能教育研究 “三个世界” 认识论方法论

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Ethanol prevents damage of skin, kidney and joint in lupus-prone mice by regulating lipid rafts

Ethanol prevents damage of skin, kidney and joint in lupus-p...

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第十一届全国免疫学学术大会

作者： Dou Hui Liu Lena Qiao Wei guo Xuanxuan deng guo-min Nanjing Medical University Beth Israel Deaconess Medical Center

Objective:Ethanol has been elaborated to have a beneficial effect on destructive ***,the effect of ethanol on the development of systemic lupus erythematosus(SLE)remains *** study was performed to determine the potent... 详细信息

关键词： Systemic lupus erythematosus Ethanol organ tissue damage Lipid rafts

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应关注系统性红斑狼疮脑组织损害的发病机制

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中国现代神经疾病杂志 2014年第9期14卷 741-743页

作者：邓国民南京医科大学基础医学院微生物学与免疫学系卫生部抗体技术重点实验室 210029

系统性红斑狼疮（SLE）是一种严重的慢性全身性自身免疫性疾病，以大量自身抗体产生和多脏器损害为主要临床特征。系统性红斑狼疮好发于青年女性，发病高峰年龄为15～40岁，男女发病比例约为1：9，其中儿童和老年患者男女发病比例约为1... 详细信息

系统性红斑狼疮（SLE）是一种严重的慢性全身性自身免疫性疾病，以大量自身抗体产生和多脏器损害为主要临床特征。系统性红斑狼疮好发于青年女性，发病高峰年龄为15～40岁，男女发病比例约为1：9，其中儿童和老年患者男女发病比例约为1：2，最近的流行病学调查资料显示全球患病率为（20～50）／10万，我国患病率约为70／10万”。

关键词：红斑狼疮系统性脑疾病核糖体蛋白质类抗原,CD3 T淋巴细胞

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维普期刊数据库

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The major roles of monocytes and their product tumor necrosis factor alpha in the induction of skin inflammation triggered by intradermal lupus IgG

The major roles of monocytes and their product tumor necrosi...

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第十一届全国免疫学学术大会

作者： Xu Guangqiong Lena Liu guo Xuanxuan Li Xiaoyan Liu Huichen deng guo-min Nanjing Medical University Beth Israel Deaconess Medical Center

Objectives: Skin injury is the second most common clinical manifestation in patients with systemic lupus erythematosus(SLE), but its pathogenesis has not been thoroughly elucidated. Methods: Based on skin deposition of IgG in SLE, we studied the features and mechanisms of intradermal IgG-induced skin inflammation. Results: We found that skin inflammation appeared at 3 h and peaked at 3 d after intradermal injection of lupus IgG. This phenomenon was related to the dose of injected IgG but not to systemic disease activity. The severity of skin inflammation induced by lupus IgG was significantly decreased in mice depleted of monocytes and in mice deficient in TNF-a but not in mice lacking mature lymphocytes. Furthermore, lupus IgG promoted the progression of monocyte differentiation to dendritic cells(DCs) and enhanced the expression of TNF-α. TNF-α was found to stimulate the IgG-induced maturation of DCs and played a major role in the proliferation and activation of keratinocytes. Conclusions: The results also indicate that the deposition of IgG in skin exerts an important role in the pathogenesis of skin injury in patients with SLE;therefore, blocking the IgG/Fc R signaling pathway can be a therapeutic target in skin lesions of patients with SLE.

关键词： Systemic lupus erythematosus lupus IgG Monocytes/macrophage TNF dendritic cells

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The role of IL-1 in skin inflammation induced by lupus serum

The role of IL-1 in skin inflammation induced by lupus serum

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第十一届全国免疫学学术大会

作者： Li Xiaoyan guo Xuanxuan Liu Huicheng Xu Guangqiong Fei Xibing deng guo-min Nanjing Medical University

Objectives: Systemic lupus erythematosus(SLE) is a seriously chronic autoimmune disease, which is characterized by a large number of autoantibodies and multiple organ damage. Skin lesion is one of the common clinical manifestations of lupus erythematosus, but its pathogenesis is not clear. IL-1 is a proinflammatory cytokine, and its role in SLE skin lesion is still ***: We used IL-1 receptor deficient mice and other gene deficient mice to study the role of IL-1 in the lupus serum-induced skin inflammation. Results: We found that the severity of skin lesion in IL-1 receptor deficient mice and caspase-1 deficient mice was reduced compared with that in wild type mice. IL-1 receptor deficiency suppressed the expression of FcγRI(CD64) and MHC class II(CD74), and increased the level of FcγRII(CD32) induced by lupus serum. IL-1 receptor deficiency also suppressed the lipid raft clustering and IFN-γ in T cells, and reduced IgG internalization and presentation in macrophage, and decreased expression of MCP-1 and TNFa in monocytes. In addition, TNFa couldpromote the proliferation of ***: Our findings indicate that IL-1 plays an important role in skin lesions of lupus erythematosus. This study suggests IL-1 is a therapeutic target in skin lesions of systemic lupus erythematosus.

关键词： Systemic lupus erythematosus Skin inflammation IL-1 lupus serum

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Intracisternal deposited IgG induces meningitis of systemic lupus erythematosus through TNFα

Intracisternal deposited IgG induces meningitis of systemic ...

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第十一届全国免疫学学术大会

作者： Xie Changhao guo Yacong Fang Xiang Qiao Wei Sun Lingyun deng guo-min Nanjing Medical University

Objective: Systemic lupus erythematosus(SLE) is a chronic autoimmune disease characterized by high levels of autoantibodies and multi-organ tissue damage including damage of the central system. Nerve system disease in SLE includes neurologic and psychiatric events, collectively termed neuropsychiatric SLE(NPSLE). Currently, the pathogenesis of NPSLE is unclear. We investigated pathogenesis of NPSLE in this ***: To understand the pathogenesis of NPSLE, we analyzed the medical records of 1131 patients with SLE, and conducted experiments by using lupus-prone mice and mice with intracisternal injections of lupus serum containing high level of autoantibody and mice with gene deficiency Results: There are 59 patients with NPSLE clinical manifestation including headaches, seizure disorder, cognitive dysfunction, cerebrovascular disease, etc. MRI examination in 14 patients with NPSLE indicates 50% normal and 50% abnormal signals. Lupus-prone mice spontaneously develop meningitis. Meningitis was developed in normal mice withintracisternal injection of lupus serum but not healthy serum;IgG is a major contributor in this meningitis. Monocyte/macrophage, complement and selectin are involved in the development of meningitis induced by lupus serum. This meningitis is dependent on dose of IgG but not associated with kinds of autoantibodies and systemic disease activity. Severity of meningitis induced by lupus serum IgG was significantly reduced in TNF deficient mice compared to wild mice. Conclusion: The deposited IgG or IgG contained immune complex in brain tissue exerts an important role in the pathogenesis of meningitis. This finding will promote development of effective therapeutic strategy to patients with NPSLE.

关键词： Systemic lupus erythematosus IgG Meningitis Monocytes/macrophages TNF

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The role of hepatic deposited IgG in development of lupus hepatic inflammation

The role of hepatic deposited IgG in development of lupus he...

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第十二届全国免疫学学术大会

作者： Fang Xiang MUHAMMAD HAIDAR ZAMAN Chen Jun-Yu deng guo-min Nanjing Medical University

Background:Systemic lupus erythematosus(SLE)is a chronic autoimmune disease characterized by high levels of autoantibodies and multi-organ tissue ***,liver damage associated with SLE is infrequently reported,but the r... 详细信息

关键词： IgG deposition Lupus hepatitis FcyR Syk

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