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Journal of Integrative Plant Biology 2005年第12期47卷 1507-1515页

作者： Neng-Guo TAO Juan XU Yun-Jiang CHENG Liu HONG Wen-Wu GUO Hua-Lin YI Xiu-xin deng State Key Laboratory of Crop Genetic Improvement National Center of Crop Molecular Breeding Huazhong Agricultural University Wuhan 430070 China

As the largest transposable element in the plant genome, retrotransposons are thought to be involved in citrus genetic instability and genome evolution, especially in sweet orange, which is prone to bud mutation. In the present study, the presence of copia-like retrotransposons, their heterogeneity, genomic distribution, and transcriptional activities in Citrus were investigated in 12 sweet orange （Citrus sinensis Osbeck） cultivars using a PCR assay designed to detect copia-like reverse transcriptase （RT） sequences. Twelve amplification products from each cultivar were cloned and sequenced. The cloned sequences showed great heterogeneity, except “Dream” navel and “Hamlin”, both of which shared the same sequence. Frame shifting, termination, deletion, and substitution accounted for the heterogeneity of RT sequences. Southern blot hybridization using the RT1 clone from the “Cara Cara” navel as a probe showed that multiple copies were integrated throughout the sweet orange genomes, which made the retrotransposon possible an effective molecular marker to detect citrus evolution events and to reveal its relationship with bud mutation. No transcriptional activities of the retrotransposon were detected by RT-PCR and Northern analysis in the fruits and leaves of either “Cara Cara” or “Seike” navels.

关键词： bud mutation Citrus cloning PCR retrotransposons.

Comparative Study on the Expression of Genes Involved in Carotenoid and ABA Biosynthetic Pathway in Response to Salt Stress in Tomato

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Journal of Integrative Agriculture 2012年第7期11卷 1093-1102页

作者： DUAN Hui-kun ZHU Yan QI Dong-dong LI Wen-long HUA Xue-jun LIU Yong-xiu deng xin Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R. China Graduate University of Chinese Academy of Sciences Beijing 100049 P.R. China Key Laboratory of Plant Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China

Carotenoid biosynthetic pathway produces not only pigments that protect photosynthetic system against photo-oxidative damage, but also precursors of abscisic acid, the major hormone regulates stress responses. To understand the response of carotenoid biosynthetic pathway to salt stress, the expression of the genes involved in carotenoid and ABA biosynthesis were compared in cultivated tomato Solanum lycopersicon cv. Moneymaker and its relative wild genotype S. pimpinellifolium （PI365967） together with the contents of carotenoids and ABA. The results showed that 11 of the 15 genes investigated were up-regulated and four unaltered in Moneymaker after 5 h of salt stress; whereas only four genes were up-regulated, four unaltered, and seven down-regulated in PI365967 after stress. Further comparison revealed that 11 salinity-induced genes were expressed significantly lower in Moneymaker than in PI365967 under normal condition, and 8 of them were induced to similar levels after salt stress. In consistence, ABA level was doubled in Moneymaker but kept consistent in PI365967 after salt stress, though the contents of neoxanthin, violaxanthin, [3-carotene, lutein, and total carotenoids were kept unchanged in both species. Since it is known that PI365967 is more tolerant to salt stress than Moneymaker, we proposed that the constitutive high level of carotenoid and ABA biosynthetic pathway under normal growth condition could be benefit to PI365967 for establishing the early response to salt stress. In addition, CrtR-bl and CrtR-b2 that encode [3-carotenoid hydroxylases were the only genes in carotenoid biosynthetic pathway that were up-regulated by salt stress in both species. The CrtR-b2 gene was cloned from both species and no essential difference was found in the encoded amino acid sequences. Transformation of CrtR-b2 to tobacco improved the seed germination under salt stress condition, indicating that the hydrolysis of β-carotenoid is the target of transcriptional regulatio

关键词： ABA carotenoid biosynthetic pathway gene expression salt stress tomato

Homogeneous-like Alkyne Selective Hydrogenation Catalyzed by Cationic Nickel Confined in Zeolite

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CCS Chemistry 2022年第3期4卷 949-962页

作者： xin deng Ruihao Bai Yuchao Chai Zhenpeng Hu Naijia Guan Landong Li School of Materials Science and Engineering&National Institute for Advanced Materials Nankai UniversityTianjin 300350 School of Physics Nankai UniversityTianjin 300071

The selective hydrogenation of alkynes to their corresponding alkenes is an important type of organic transformation,which is currently accomplished by modified palladium ***,we report that coordinatively unsaturated Ni(II)sites confined in faujasite zeolite,that is,Ni@Y,can efficiently catalyze the selective hydrogenation of various alkynes in both gas-and liquid *** and kinetic analyses explicitly reveal that alkyne hydrogenation over Ni@Y follows the homogeneous associative mechanism instead of the classic heterogeneous Horiuti–Polanyi *** functional theory calculations confirm that the preferential adsorption of alkynes on coordinatively unsaturated Ni(II)sites is crucial to initiate dihydrogen activation for the subsequent ***@Y represents a true heterogeneous catalyst acting as a homogeneous catalyst and shares the advantages of both heterogeneous and homogeneous *** findings may shed light on the rational design of robust catalysts and new catalytic routes by linking heterogeneous and homogeneous catalysis.

关键词： zeolites alkynes selective hydrogenation associative mechanism homogeneous-like

Boosting ammonia production in electrocatalytic NO_(x)reduction on a robust Fe/FeMoO_(4)catalyst

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Nano Research 2024年第7期17卷 5801-5806页

作者： Dong-Xue Liu xin deng Yong-Fu Zhu Zhe Meng Xue-Feng Sun Miao-Miao Shi Hai-Xia Zhong Jun-Min Yan Key Laboratory of Automobile Materials Ministry of EducationSchool of Materials Science and EngineeringJilin UniversityChangchun 130022China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

Electrocatalytic reduction of nitrate(NO_(3)^(−))and nitride(NO_(2)^(−))to ammonia(NH3)is of wide interest as a promising alternative to the energy-intensive Haber-Bosch route for mitigating the vast energy consumption and the accompanied carbon dioxide emission,as well as benefiting for the relevant sewage ***,exploring an efficient and low-cost catalyst with high atomic utilization that can effectively facilitate the slow multi-electron transfer process remains a grand ***,we present an efficient hydrogenation of NO_(3)^(−)/NO_(2)^(−)species to NH3 in both alkaline and neutral environments over the Fe_(2)(MoO_(4))_(3)derived hybrid electrocatalyst with the metallic Fe site on FeMoO_(4)(Fe/FeMoO_(4)).The Mo ingredient can play a synergistically positive role in further promoting the NH_(3) production on *** a result,Fe/FeMoO_(4)behaves well in the electrochemical NH_(3) generation from NO_(2)^(−)with a maximum NH_(3) Faradaic efficiency(FE)of 96.53%and 87.68%in alkaline and neutral electrolyte,corresponding to the NH_(3) yield rate of 640.68 and 302.56 mg·h^(−1)·mg_(cat.)^(−1),respectively,which outperforms the Fe and Mo counterpart and other similar catalyst,showing the robust catalytic capacity of each active site.

关键词： ammonia synthesis NO_(x)reduction synergistic effect alkaline/neutral electrolyte

Decreased KCNE2 expression participates in the development of cardiac hypertrophy

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中国病理生理杂志 2016年第8期32卷 1499-1500页

作者： deng Jian-xin LIU Wen-juan DING Wen-wen WANG Gang LIU Jie Department of Pathophysiology School of MedicineShenzhen University

AIM: To investigate whether KCNE2 participates in the development of pathological hypertrophy. METHODS: Bidirectional manipulations of KCNE2 expression were performed by adenoviral overexpression of KCNE2 or knockdown of KCNE2 with RNA interference in PE-induced neonatal rat ventricular myocytes. Then overexpression of KCNE2 in mouse model of left ventricular hypertrophy induced by transverse aortic constriction( TAC) by ultrasound microbubble-mediated gene transfer were used to detect the therapeutic function of KCNE2 in the development of hypertrophy. RESULTS: KCNE2 expression was significantly decreased in PE-induced hypertrophic cardiomyocytes and in hypertrophic hearts produced by TAC. Knockdown of KCNE2 in cardiomyocytes reproduced hypertrophy,whereas overexpression of KCNE2 attenuated PE-induced cardiomyocyte hypertrophy. Knockdown of KCNE2 increased calcineurin activity and nuclear NFAT protein level,and pretreatment with nifedipine or FK506 attenuated decreased KCNE2-induced cardiomyocyte hypertrophy. Overexpression of KCNE2 in heart by ultrasound microbubble-mediated gene transfer suppressed the development of hypertrophy and activation of calcineurin-NFAT and MAPK pathways in TAC mice. CONCLUSION: These findings demonstrate that cardiac KCNE2 expression is decreased and contributes to the development of hypertrophy via activation of calcineurin-NFAT and MAPK pathways.

关键词： KCNE Decreased KCNE2 expression participates in the development of cardiac hypertrophy

Efficacy and safety of Traditional Chinese Medicine for the treatment of acquired immunodeficiency syndrome:a systematic review

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Journal of Traditional Chinese Medicine 2014年第1期34卷 1-9页

作者： xin deng Manjun Jiang Xiaofang Zhao Jian Liang Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine

OBJECTIVE:To systematically review the efficacy and safety ofTraditional Chinese Medicine(TCM)interventions,compared with control interventions(placebo or conventional Western medical therapy),in the treatment of acquired immunodeficiency syndrome(AIDS).METHODS:Electronic databases including PubMed,the Cochrane Library,China National Knowledge Infrastructure(CNKI),and Wanfang Data were searched to identify relevant randomized controlled trials(RCTs)published as of May *** were selected according to the specified inclusion and exclusion criteria and then subjected to methodological quality assessment,data extraction,and meta-analysis according to the Cochrane Handbook for Systematic Reviews of ***:Twelve RCTs involving 881 patients with AIDS were *** quality assessment showed that two were high-quality,two were moderate-quality,and eight were ***-analysis showed that TCM interventions were associated with significantly reduced plasma viral load compared with placebo[odds ratio OR=2.46,95%confidence interval CI(1.02,5.94);P=0.04].However,the reductions in plasma viral load significantly favored conventional Western medical therapy alone over integrated traditional Chinese and Western medical therapy[OR=0.16,95%CI(0.05,0.55);P=0.004].Patients receiving TCM interventions had significantly higher CD4+T lymphocyte counts compared with those on placebo[OR=2.54,95%CI(1.40,4.60);P=0.002].In addition,TCM interventions were significantly more likely to have improved clinical symptoms[OR=2.82,95%CI(1.85,4.31);P<0.00001].TCM interventions conferred a similar risk of adverse events(AEs)compared with control interventions[OR=1.87,95%CI(0.58,6.01);P=0.29].CONCLUSION:Current evidence suggests that TCM interventions are significantly more effective than placebo in reducing plasma viral load and increasing CD4+T lymphocyte count in patients with *** compared with conventional Western medical therapy,TCM interventions we

关键词： Acquired immunodeficiency syn-drome Traditional Chinese Medicine Safety Sys-tematic review

Protective Effect of Fresh/Dry Dandelion Extracts on APAP-Overdose-Induced Acute Liver Injury

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Chinese Journal of Integrative Medicine 2022年第8期28卷 683-692页

作者： ZHENG Yao LEI Lei LIANG Shuai AI Jiao deng xin LI Yan-qiu ZHANG Tian-pei PU Shi-biao REN Yong-shen School of Pharmaceutical Science South-Central University for NationalitiesWuhan430074China College of Chinese Materia Medica Yunnan University of Chinese MedicineKunming650500China

Objecitvie:To compare the liver protective activity of fresh/dried dandelion extracts against acetaminophen(APAP)-induced ***:Totally 90 Kunming mice were randomly divided into10 groups according to body weight(9 mice for each group).The mice in the normal control and model(vehicle control)groups were administered sodium carboxymethyl cellulose(CMC-Na,0.5%)*** groups were pretreated with high and low-dose dry dandelion extract(1,000 or 500 g fresh herb dried and then decocted into 120 mL solution,DDE-H and DDE-L);low-,medium-and high-dose dandelion juice(250,500,1,000 g/120 mL,DJ-L,DJ-M,and DJ-H);fresh dandelions evaporation juice water(120 mL,DEJW);dry dandelion extract dissolved by pure water(1 kg/120 mL,DDED-PW);dry dandelion extract dissolved by DEJW(120 g/120 mL,DDED-DEJW)by oral gavage for 7 days at the dosage of 0.5 mL solution/10 g body weight;after that,except normal control group,all other groups were intraperitonealy injected with 350 mg/kg APAP to induce liver *** hours after APAP administration,serum and liver tissue were collected and serum alanine aminotransferase(AST),aspartate transaminase(ALT),alkaline phosphatase(AKP),malondialdehyde(MDA),glutathione(GSH),superoxide dismutase(SOD)activities were quantified by biochemical kits;tumor necrosis factor(TNF-α),interleukin(IL)-2,and IL-1β contents in liver tissue were determined by enzyme linked immunosorbent assay *** changes in liver tissues were observed by hematoxylin and eosin staining;TUNEL Assay and Hoechst 33258 staining were applied for cell apoptosis *** expressions of heme oxygenase-1(HO-1),nuclear factor erythroid-2-related factor 2(Nrf-2),caspase-9,B-cell leukemia/lymphoma 2(Bcl-2),Bax and p-JNK were determined by Western blot ***:Pretreatment with fresh dandelion juice(FDJ,including DJ-L,DJ-M,DJ-H,DEJW and DDED-DEJW)significantly decreased the levels of serum ALT,AST,AKP,TNF-α and IL-1β compared with veh

关键词： fresh dandelion juice acetaminophen-induced liver injury oxidative stress anti-inflammation anti-apoptosis

Role of Co Content on Densification and Microstructure of WC–Co Cemented Carbides Prepared by Selective Laser Melting

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Acta Metallurgica Sinica(English Letters) 2021年第9期34卷 1245-1254页

作者： Jinyang Liu Jian Chen Li Zhou Bingyao Liu Yang Lu Shanghua Wu xin deng Zhongliang Lu Zhipeng Xie Wei Liu Jianye Liu Zhi Qu School of Electromechanical Engineering Guangdong University of TechnologyGuangzhou 510006China School of Electromechanical Engineering Guangdong Polytechnic Normal UniversityGuangzhou 510635China Master Group(Guangdong)Technology Research Co LtdYunfu 527400China State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049China Guangdong Xi’an Jiaotong University Academy Foshan 528300China State Key Laboratory of New Ceramics and Fine Processing Tsinghua UniversityBeijing 100091China Guangdong Hanbang 3D Technology Co. LtdZhongshan 528427China Guangdong Fenghua Zhuoli Technology Co. LtdFoshan 528225China

WC–Co cemented carbides, well-known as the conventional tooling materials, have not been successfully produced by one step additive manufacturing processes such as selective laser melting(SLM) yet. The microstructure evolution as well as WC grain growth behavior has rarely been investigated in detail during SLM process. In this study, the WC–Co cemented carbides with different Co contents(12–32 wt%) were prepared by optimized SLM processes for comparative investigation of densification behavior, microstructure characterization and mechanical property. The increase in Co content in feedstock carbide granules can improve the densification behavior during SLM process. The SLM processed WC-12 Co shows larger average WC grain size and higher percentage of coarser WC grains as compared with both WC-20 Co and WC-32 Co. The microstructure characterization, combined with finite element simulation, shows the WC grain growth mechanisms include agglomeration and dissolution-deposition of WC during SLM process and agglomeration of WC is an important mechanism especially for WC–Co cemented carbides with Co content as low as 12 wt%. The comparison between horizontal(perpendicular to the SLM laser beam) and vertical(parallel to the SLM laser beam) cross sections of carbides shows that SLM process introduces a certain degree of microstructure and mechanical behavior anisotropy for WC-12 Co, WC-20 Co, and WC-32 Co.

关键词： Cemented carbides Selective laser melting Microstructure Densifi cation behavior Finite element simulation

A New Method for Thin Film Deposition-Faced Microwave Electron Cyclotron Resonance Plasma Sources Enhanced Direct-Current Magnetron Sputtering

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Chinese Physics Letters 2000年第8期17卷 586-588页

作者： XU Jun MA Teng-cai LU Wen-qi XIA Yuan-liang deng xin-lu State Key Laboratory for Materials Modification by Laser Ionand Electron BeamsDepartment of PhysicsDalian University of TechnologyDalian 116024

A new reactive magnetron sputtering system enhanced by the faced microwave electron cyclotron resonance plasma source was designed and amorphous CN_(x) films has been prepared by using this *** character ization of the films by interference microscopy,atomic force microscopy,and x-ray photoelectron spectroscopy shows that the deposition rate is strongly affected by the direct-current bias,and the films are composed by a single carbon nitride phase and the N/C ratio is 4:3.2,which is close to that of C_(3)N_(4)(4:3).

关键词： Electron Plasma microwave

Two Lycopene β-Cyclases Genes from Sweet Orange (Citrus sinensis L. Osbeck) Encode Enzymes With Different Functional Efficiency During the Conversion of Lycopene-to-Provitamin A

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Journal of Integrative Agriculture 2013年第10期12卷 1731-1747页

作者： ZHANG Jian-cheng ZHOU Wen-jing XU Qiang TAO Neng-guo YE Jun-li GUO Fei XU Juan deng Xiu-xin Key Laboratory of Horticultural Plant Biology Ministry of Education/College of Horticulture and ForestryHuazhong Agricultural University

Citrus fruits are rich in *** the carotenoid biosynthetic pathway,lycopene β-cyclase（LCYb,EC：1.14.-.-） is a key regulatory enzyme in the catalysis of lycopene to β-carotene,an important dietary precursor of vitamin A for human *** closely related lycopene β-cyclase cDNAs,designated CsLCYb1 and CsLCYb2,were isolated from the pulp of orange fruits（Citrus sinensis）.The expression level of CsLCYb genes is lower in the flavedo and juice sacs of a lycopeneaccumulating genotype Cara Cara than that in common genotype Washington,and this might be correlated with lycopene accumulation in Cara Cara *** CsLCYb1 efficiently converted lycopene into the bicyclic β-carotene in an Escherichia coli expression system,but the CsLCYb2 exhibited a lower enzyme activity and converted lycopene into the β-carotene and the monocyclic γ-*** tomato transformation studies,expression of CsLCYb1 under the control of the cauliflower mosaic virus（CaMV） 35S constitutive promoter resulted in a virtually complete conversion of lycopene into β-carotene,and the ripe fruits displayed a bright orange ***,the CsLCYb2 transgenic tomato plants did not show an altered fruit colour during development and *** fruits of the CsLCYb1 transgenic plants,most of the lycopene was converted into β-carotene with provitamin A levels reaching about 700 μg ***,most transgenic tomatoes showed a reduction in total carotenoid accumulation,and this is consistent with the decrease in expression of endogenous carotenogenic genes in transgenic ***,these results suggested that the cloned CsLCYb1 and CsLCYb2 genes encoded two functional lycopene β-cyclases with different catalytic efficiency,and they may have potential for metabolite engineering toward altering pigmentation and enhancing nutritional value of food crops.

关键词： carotenoids biosynthesis citrus fruit gene expression lycopene lycopene β-cyclase β-Carotene transgenic tomato fruit