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Emergence of H5N1 highly pathogenic avian influenza in Democratic People's Republic of Korea

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Journal of Integrative Agriculture 2022年第5期21卷 1534-1538页

作者： LIU Li-ling YANG huan-liang guo Fu-sheng WANG Xiu-rong deng guo-hua SHI Jian-zhong TIAN guo-bin ZENG Xian-ying State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China China Animal Health and Epidemiology Center Qingdao 266032P.R.China

In the past decade,there has been extensive global surveillance for highly pathogenic avian influenza(HPAI)infection in both animals and humans,however,few studies on epidemiology of avian influenza in Democratic People’s Republic of Korea(DPRK)were *** the period 2013–2014,HPAI H5N1 viruses were detected with outbreaks in domestic poultry in *** analysis revealed that the hemagglutinin gene of all samples belonged to clade 2.***.2.1c with high *** HPAI H5N1 virus found in ducks at the Tudan Duck Farm in 2013 was might introduced by migratory birds and then led to the outbreaks on neighboring chicken farms in *** data provide direct evidence for the transmission of avian influenza viruses from wild birds to waterfowl to terrestrial ***,the monitoring and control of influenza virus in ducks must be given top priority,which are essential components to prevent and control HPAI.

关键词： avian influenza H5N1 Democratic People’s Republic of Korea clade 2.3.2.1c

Development of a real-time RT-PCR method for the detection of newly emerged highly pathogenic H7N9 influenza viruses

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Journal of Integrative Agriculture 2017年第9期16卷 2055-2061页

作者： WANG Xiu-rong GU Lin-lin SHI Jian-zhong XU Hai-feng ZHANG Ying ZENG Xian-ying deng guo-hua LI Cheng-jun CHEN hua-lan State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

In 2013, a human influenza outbreak caused by a novel H7N9 virus occurred in China. Recently, the H7N9 virus acquired multiple basic amino acids at its hemagglutinin（HA） cleavage site, leading to the emergence of a highly pathogenic virus. The development of an effective diagnostic method is imperative for the prevention and control of highly pathogenic H7N9 influenza. Here, we designed and synthesized three pairs of primers based on the nucleotide sequence at the HA cleavage site of the newly emerged highly pathogenic H7N9 influenza virus. One of the primer pairs and the corresponding probe displayed a high level of amplification efficiency on which a real-time RT-PCR method was established. Amplification using this method resulted in a fluorescent signal for only the highly pathogenic H7N9 virus, and not for any of the H1–H15 subtype reference strains, thus demonstrating high specificity. The method detected as low as 39.1 copies of HA-positive plasmid and exhibited similar sensitivity to the virus isolation method using embryonated chicken eggs. Importantly, the real-time RT-PCR method exhibited 100% consistency with the virus isolation method in the diagnosis of field samples. Collectively, our data demonstrate that this real-time RT-PCR assay is a rapid, sensitive and specific method, and the application will greatly aid the surveillance, prevention, and control of highly pathogenic H7N9 influenza viruses.

关键词： H7N9 highly pathogenic influenza virus real-time RT-PCR

Protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses

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Journal of Integrative Agriculture 2022年第7期21卷 2086-2094页

作者： ZENG Xian-ying HE Xin-wen MENG Fei MA Qi WANG Yan BAO Hong-mei LIU Yan-jing deng guo-hua SHI Jian-zhong LI Yan-bing TIAN guo-bin CHEN hua-lan State Key Laboratory of Veterinary Biotechnology National Poultry Laboratory Animal Resource CenterHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.***.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.***.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.***.4.4 b HA gene, an H5 N6 virus bearing the clade 2.***.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.***.4.4 h or 2.***.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.

关键词： avian influenza H5/H7 trivalent vaccine H5-Re13 H5-Re14 H7-Re4 strains protective efficacy chickens ducks and geese

Electrochemical anodic oxidation assisted fabrication of memristors

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International Journal of Extreme Manufacturing 2024年第3期6卷 250-272页

作者： Shuai-Bin hua Tian Jin Xin guo School of Materials Science and Engineering State Key Laboratory of Material Processing and Die&Mould TechnologyHuazhong University of Science and TechnologyWuhan 430074People’s Republic of China

Owing to the advantages of simple structure,low power consumption and high-density integration,memristors or memristive devices are attracting increasing attention in the fields such as next generation non-volatile memories,neuromorphic computation and data ***,the deposition of memristive films often requires expensive equipment,strict vacuum conditions,high energy consumption,and extended processing *** contrast,electrochemical anodizing can produce metal oxide films quickly(e.g.10 s) under ambient *** means of the anodizing technique,oxide films,oxide nanotubes,nanowires and nanodots can be fabricated to prepare *** film thickness,nanostructures,defect concentrations,etc,can be varied to regulate device performances by adjusting oxidation parameters such as voltage,current and *** memristors fabricated by the anodic oxidation technique can achieve high device consistency,low variation,and ultrahigh yield *** article provides a comprehensive review of the research progress in the field of anodic oxidation assisted fabrication of ***,the principle of anodic oxidation is introduced;then,different types of memristors produced by anodic oxidation and their applications are presented;finally,features and challenges of anodic oxidation for memristor production are elaborated.

关键词： anodic oxidation anodized aluminium oxide memristor resistive switching electrical properties

On the convergence of tracking differentiator with multiple stochastic disturbances

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Science China(Information Sciences) 2024年第2期67卷 157-169页

作者： Zehao WU huacheng ZHOU Baozhu guo Feiqi deng School of Mathematics and Big Data Foshan University School of Mathematics and Statistics Central South University Academy of Mathematics and Systems Science Chinese Academy of Sciences Systems Engineering Institute South China University of Technology

This paper investigates the convergence, noise-tolerance, and filtering performance of a tracking differentiator in the presence of multiple stochastic disturbances for the first time. We consider a general case wherein the input signal is corrupted by additive colored noise, and the tracking differentiator is disturbed by additive colored noise and white noise. The tracking differentiator is shown to track the input signal and its generalized derivatives in the mean square sense. Further, the almost sure convergence can be achieved when the stochastic noise affecting the input signal is vanishing. Herein, numerical simulations are performed to validate the theoretical results.

关键词： tracking differentiator convergence noise-tolerance filtering performance multiple stochastic disturbances

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

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Journal of Integrative Agriculture 2020年第9期19卷 2294-2300页

作者： ZENG Xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing deng guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang guo Xing-fu LIU Jing-li TIAN guo-bin CHEN hua-lan State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China Harbin Weike Biotechnology Development Company Harbin 150069P.R.China

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.***.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.***.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.

关键词： avian influenza H5/H7 trivalent vaccine Re-11 Re-12 and H7-Re2 strains protective efficacy

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黄土隧道围岩的结构性变化特征分析

岩土工程学报 2008年第2期30卷 219-224页

作者：邓国华邵生俊西安理工大学岩土工程研究所陕西西安710048

在分析和修正已有结构性参数的基础上,首次提出了将结构性参数直接应用于工程实践的思路。研究了黄土隧道开挖后围岩结构性的变化规律。研究得出:围岩的结构性参数与径深和洞径密切相关;随着径深的增大,拱顶方向上围岩的结构性参数均呈... 详细信息

在分析和修正已有结构性参数的基础上,首次提出了将结构性参数直接应用于工程实践的思路。研究了黄土隧道开挖后围岩结构性的变化规律。研究得出:围岩的结构性参数与径深和洞径密切相关;随着径深的增大,拱顶方向上围岩的结构性参数均呈现出先减小后增大的规律,存在一个最小值。与开挖前原状土的结构性参数相比,围岩的结构性损失在距拱顶约1.7～2.5 m时达到最大。随着洞径的增大,开挖对围岩黄土的扰动增强,拱顶方向上围岩的结构性减弱,结构性参数的最小值变化不大,但最小值发生的位置逐渐远离拱顶。最后,提出了以围岩结构性损失最大为依据判断围岩松动圈的新方法。分析表明,黄土隧道围岩的松动圈在1.7～2.5 m变化。

关键词：黄土隧道结构性参数围岩松动圈

Microstructure and property of laser additive manufactured alloy Ti-6Al-2V-1.5Mo-0.5Zr-0.3Si after aged at different temperatures

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Rare Metals 2024年第5期43卷 2275-2281页

作者： guo-Chao Li Xu Cheng hua-Ming Wang National Engineering Laboratory of Additive Manufacturing for Large Metallic Components and Engineering Research Center of Ministry of Education on Laser Direct Manufacturing for Large Metallic Components School of Materials Science and EngineeringBeihang UniversityBeijing 100191China

The solid solution and aging treatment for conventional manufacturing processes might not be suitable for laser additive manufactured titanium alloys due to the different lamellar *** this study,the influence of aging temperatures(600,700 and 800°C)on microstructure and mechanical properties of titanium alloy Ti-6Al-2V-1.5Mo-0.5Zr-0.3Si was *** results indicate that after solid solution treatment at 970°C followed by water quenching,the alloy mainly consists of coarsening lamellar a phase in martensite α' *** at 600°C will not change the size of primary lamellar α phase but lead to huge amount of secondary a phases(α_(s))generating with very fine *** increasing the aging temperature,the number of α_(s) decreases but with coarsened *** aged at 800°C,the width of the asphase reaches 350 nm,almost 7 times wider than that aged at 600°*** changing size of α_(s) obviously influences the property of the *** fine α_(s) leads to high strength and microhardness but low plasticity,and specimen aged at 700°C with suitable assize has the best comprehensive properties.

关键词： α+βtitanium alloy Laser additive manufacturing Solid solution and aging treatment Microstructure Room temperature tensile property

Recent advances in cobalt phosphide-based materials for electrocatalytic water splitting:From catalytic mechanism and synthesis method to optimization design

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Nano Materials Science 2024年第2期6卷 139-173页

作者： Rongrong deng Mengwei guo Chaowu Wang Qibo Zhang Key Laboratory of Ionic Liquids Metallurgy Faculty of Metallurgical and Energy EngineeringKunming University of Science and TechnologyKunming650093PR China State Key Laboratory of Complex Nonferrous Metal Resources Cleaning Utilization in Yunnan Province Kunming650093PR China

Electrochemical water splitting has long been considered an effective energy conversion technology for trans-ferring intermittent renewable electricity into hydrogen fuel,and the exploration of cost-effective and high-performance electrocatalysts is crucial in making electrolyzed water technology commercially *** phosphide(Co-P)has emerged as a catalyst of high potential owing to its high catalytic activity and durability in water *** paper systematically reviews the latest advances in the development of Co-P-based materials for use in water *** essential effects of P in enhancing the catalytic performance of the hydrogen evolution reaction and oxygen evolution reaction are first ***,versatile synthesis techniques for Co-P electrocatalysts are summarized,followed by advanced strategies to enhance the electrocatalytic performance of Co-P materials,including heteroatom doping,composite construction,integration with well-conductive sub-strates,and structure control from the viewpoint of *** with these optimization strategies,the understanding of the inherent mechanism of enhanced catalytic performance is also ***,some existing challenges in the development of highly active and stable Co-P-based materials are clarified,and pro-spective directions for prompting the wide commercialization of water electrolysis technology are proposed.

关键词： Co-P electrocatalysts Water splitting Hydrogen production Catalytic mechanism Synthesis technique Optimization design