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Effects of redox potential on soil cadmium solubility:Insight into microbial community

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Journal of Environmental Sciences 2019年第1期31卷 224-232页

作者： delong meng Juan Li Tianbo Liu Yongjun Liu Mingli Yan Jin Hu Xiaoqi Li Xueduan Liu Yili Liang Hongwei Liu Huaqun Yin School of Minerals Processing and Bioengineering Central South University Key laboratory of Biometallurgy Ministry of Education College of Agronomy Hunan Agricultural University College of Plant protection Hunan Agricultural University School of Life Science Hunan University of Science and Technology

Understanding the role of microbes in the solubility of cadmium(Cd) is of fundamental importance for remediation of Cd toxicity. The present study aimed to identify the microbes that involved in regulating Cd solubility and to reveal possible mechanisms. Therefore,microbial communities were investigated through high-throughput sequencing approach, the molecular ecological network was constructed and metagenomes were predicted. Our results indicated that redox conditions affected both the solubility of soil Cd and the microbial communities. Anaerobic microbes, such as Anaerolineaceae, did not only play important roles in shaping the microbial community in soils, but might also be involved in regulating the Cd solubility. Two possible mechanisms that how Anaerolineaceae involved in Cd solubility are(1) Anaerolineaceae are important organic matter degraders under anoxic conditions and(2) Anaerolineaceae can co-exist with methane metabolism microbes, while methane metabolism promotes the precipitation of soluble Cd. Thus, application of Anaerolineaceae in bioremediation of soil Cadmium contamination is a potential approach. The study provided a novel insight into the role of microbial community in the regulation of Cd solubility under different redox conditions, and suggested a potential approach for the remediation of soil Cd contamination.

关键词： Cd solubility Redox potential Microbial community Molecular ecology networks (MENs) Anaerolineaceae

Scene Of Spring--Jiangsu Province China

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ChinAfrica 2021年第4期13卷 4-5页

作者： meng delong(Photograph) 不详

Jiangsu Province China Tourists take a boat tour in Qianduo Scenic Spot in Xinghua,east China’s Jiangsu Province,on March *** Rape Flower Tourism Festival kicked off on the day at the *** can enjoy a vibrant display ... 详细信息

关键词： flowers Jiangsu Spring

Determining the biological zero for gonadal development razor clams Sinonovacula constricta (lamarck 1818) in Zhejiang,China

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Aquaculture and Fisheries 2021年第1期6卷 56-62页

作者： Baobao Xue delong meng Hao Li Wei Liang Donghong Niu Jiale Li Heding Shen Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics Breeding Shanghai201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China

The biological zero of gonadal development and the effective accumulated temperature of one and two-year-old razor clams Sinonovacula constricta(Lamarck 1818)were studied in Sanmen County,Zhejiang Province,China between April 2016 to October 2017,using a new variety named“Shen-Zhe No.1”.The biological zero was determined by using the rule of effective accumulated temperature and the linear regression method,which was confirmed using a histological analysis of the full gonadal development *** results showed that the biological zero of gonadal reproduction of 1-year-old and 2-year-old *** was the same:20.8℃.When the temperature reached 20–21℃,analysis of the histological sections indicated that more than half of the *** individuals initiated gonadal *** average effective accumulated temperature over two years was 806 d℃.This study contributes to our understanding of the regulation and control of gonadal maturation,suggesting that changes in the spawning and breeding season during one-year of farming using the new variety of razor clam could provide an economic benefit to industrialized large-scale spat *** results demonstrated that the razor clam *** is a typical clam of the accumulative temperature mature oviposition *** addition,although *** does not display hypothermy,it requires a lower temperature for gonadal maturity and spawning.

关键词： Sinonovacula constricta Biological zero Gonads Development Histology

Effects of solid-state fermentation product of yeast supplementation on liver and intestinal health,and resistance of common carp(Cyprinus carpio)against spring viraemia carp virus

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Animal Nutrition 2024年第3期18卷 408-418页

作者： mengxin Wang Dongmei Xia Lijuan Yu Qiang Hao Mingxu Xie Qingshuang Zhang Yajie Zhao delong meng Yalin Yang Chao Ran Tsegay Teame Zhen Zhang Zhigang Zhou China-Norway Joint Lab on Fish Gut Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Hubei Key Laboratory of Animal Nutrition and Feed Science Wuhan Polytechnic UniversityWuhan 430000China Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Tigray Agricultural Research Institute Mekelle Agricultural Research CenterMekelleTigray 251Ethiopia

This study aimed to investigate the effects of solid-state fermentation products of yeast(SFPY)on liver and intestinal health and disease resistance of common carp(Cyprinus carpio).A total of 200 common carp with an initial average weight of 2.55±0.004 g were divided into 5 groups(4 replications per group and 10 fish per replication),and were fed with one of five diets,including a control diet and 4 diets supplemented with 2‰(Y2),3‰(Y3),4‰(Y4),or 5‰(Y5)SFPY,respectively,for 8 *** indicated that,the addition of SFPY to the diet of common carp did not affect the growth performance or survival rate of fish(P=0.253).Interestingly,with the addition of SFPY,the triacylglycerol(TAG)content of the liver presented a linear decreasing tendency(P=0.004),with significantly decreased in Y4 and Y5 groups(P=0.035)compared with *** lipopolysaccharide(LPS)content and diamine oxidase(DAO)activity presented a negative linear relationship with the addition of SFPY(P=0.015,P=0.030),while serum lipopolysaccharide binding protein(LBP)content first decreased and then increased(P<0.001).The total antioxidant capacity(T-AOC)in the intestine of fish increased continuously with increasing SFPY supplementation(P=0.026),reaching the highest level in Y5 *** villus height in all experimental groups were significantly higher than that in the control group(P<0.001).Furthermore,compared to the control,adding 3‰SFPY to the control diet of common carp significantly increased the relative abundance of Fusobacteria(P=0.018)and decreased that of Proteobacteria(P=0.039)at phylum level,and increased the relative abundance of Cetobacterium(P=0.018)and decreased that of Shewanella(P=0.013)at genus *** with the control,the relative mRNA expression level of spring viraemia of carp virus N protein(SVCV-n)in the kidney was lower than that of the control group without significance and bottomed out in Y4 group(P=0.138).In conclusion,dietary SFPY enhanced the SVCV resistance capa

关键词： Yeast Solid-state fermentation product Probiotic Immunity Gut microbiota Common carp

A Preliminary Study of the Key Technologies in Rice Seed Production

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Asian Agricultural Research 2015年第12期7卷 90-92页

作者： Guodong HUA Guanxi LI delong meng Duoming WANG Lianyungang Academy of Agricultural Sciences

To study the production technology of rice seeds,with Lianjing 7 as test material,this paper explores the effects of the key rice seed production,processing and storage technologies( such as nitrogen fertilizer application and management,weeding mode in seed breeding field,temperature control of mechanical drying and water control on seed storage) on the quality of rice seeds. The results show that the optimal application proportion of tillering stage dressing and earing fertilizer is 8: 2,followed by 7 : 3; the best time of weeding is booting stage,with the highest purity; the effect of drying and germination rate is best when the temperature is controlled at 44℃ in mechanical drying process; the seed storage quality and storage costs can be guaranteed when the water content of rice seed is controlled at 18%.

关键词： Rice seed Nitrogen fertilizer application and management Weeding period Mechanical drying Water content

Biocontrol agents modulate phyllosphere microbiota interactions against pathogen Pseudomonas syringae

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Environmental Science and Ecotechnology 2024年第5期21卷 309-317页

作者： Zhaoyue Yang Tianbo Liu Jianqiang Fan Yiqiang Chen Shaolong Wu Jingjing Li Zhenghua Liu Zhendong Yang Liangzhi Li Suoni Liu Hongwu Yang Huaqun Yin delong meng Qianjun Tang College of Plant Protection Hunan Agricultural UniversityChangsha410128HunanChina School of Minerals Processing and Bioengineering Central South UniversityChangsha410083HunanChina Hunan Tobacco Research Institute Changsha410004HunanChina Technology Center Fujian Tobacco Industrial Co.Ltd.Xiamen361000FujianChina School of Architecture and Civil Engineering Chengdu UniversityChengdu610106SichuanChina Yongzhou Tobacco Corporation Yongzhou425000HunanChina

The pathogen Pseudomonas syringae,responsible for a variety of diseases,poses a considerable threat to global crop *** biocontrol strategies employ antagonistic microorganisms,utilizing phyllosphere microecology and systemic resistance to combat this ***,the interactions between phyllosphere microbial dynamics and the activation of the plant defense system remain poorly *** we show significant alterations in phyllosphere microbiota structure and plant gene expression following the application of biocontrol *** reveal enhanced collaboration and integration of Sphingomonas and Methylobacterium within the microbial co-occurrence ***,Sphingomonas inhibits *** by disrupting pathogen chemotaxis and ***,both Sphingomonas and Methylobacterium activate plant defenses by upregulating pathogenesis-related gene expression through abscisic acid,ethylene,jasmonate acid,and salicylic acid signaling *** results highlighted that biocontrol agents promote plant health,from reconstructing beneficial microbial consortia to enhancing plant *** findings enrich our comprehension of the synergistic interplays between phyllosphere microbiota and plant immunity,offering potential enhancements in biocontrol efficacy for crop protection.

关键词： Biological control Metagenome Transcriptome Community structure Pathogen resistance

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一种用于存储与查询半结构化数据的新方法

计算机工程 2006年第19期32卷 91-93页

作者：叶飞跃蒙德龙员红娟上海大学计算机工程与科学学院上海200072

由于半结构化数据缺乏模式信息,因而半结构化数据的存储与查询将是一个十分重要且具有挑战性的研究课题。利用关系数据库存储半结构化数据可以重用数据库的查询优化器和事务处理机制,能够保证半结构化数据的一致性和完整性。该文提出一... 详细信息

由于半结构化数据缺乏模式信息,因而半结构化数据的存储与查询将是一个十分重要且具有挑战性的研究课题。利用关系数据库存储半结构化数据可以重用数据库的查询优化器和事务处理机制,能够保证半结构化数据的一致性和完整性。该文提出一种实现半结构化数据存储与查询的新方法,该方法使用关系数据库系统来实现半结构化数据的存储与查询。给出了把基于半结构化数据的查询重写为基于关系的查询的算法,同时介绍一个可视化查询程序。

关键词：半结构化数据查询OEM

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虾夷扇贝分拣柔性抓取装置设计与试验

大连海洋大学学报 2023年第6期38卷 1044-1051页

作者：蔡卫国孟德龙郭晟翰大连海洋大学机械与动力工程学院辽宁大连116023 辽宁省渔业装备工程技术研究中心辽宁大连116023

为解决传统生产中海珍品分拣方式效率低、破损率高的实际问题,以虾夷扇贝(Patinopecten yessoensis)为对象,在对其形态特性、物理特性研究的基础上,研制了一种三指柔性抓取装置,柔性手指呈“A”字型结构,柔性三指张开度Ф为14.66~197.44... 详细信息

为解决传统生产中海珍品分拣方式效率低、破损率高的实际问题,以虾夷扇贝(Patinopecten yessoensis)为对象,在对其形态特性、物理特性研究的基础上,研制了一种三指柔性抓取装置,柔性手指呈“A”字型结构,柔性三指张开度Ф为14.66~197.44 mm,并建立柔性手指模型,将虾夷扇贝实体简化为类球体,对柔性单指受力和柔性三指抓取进行仿真分析。结果表明:设计的分拣装置柔性单指受力时向接触面受力一侧弯曲,柔性三指弯曲变形组成完整的包络曲面,包络自适应性能良好;对单一规格虾夷扇贝进行抓取成功率和夹持力试验显示,当气压低于0.2 MPa时,抓取成功率不足90%,当气压达到0.6 MPa时,平均抓取成功率在98%以上,壳体及边缘均无破损,且随着气压的增加,抓取成功率和夹持力均呈正相关变化;在气压为0.6 MPa时,对3种不同质量的虾夷扇贝进行抓取性能试验显示,实测横向、纵向抓取的最大夹持力分别为8.76、7.24 N,虾夷扇贝壳体及边缘无破损,且随着虾夷扇贝质量的增加,夹持力呈下降趋势。研究表明,本研究中所设计的分拣装置能够满足大部分贝类珍品分拣的需求,可应用于规模化生产扇贝、牡蛎、鲍和螺等多种贝类珍品的无损分拣。

关键词：虾夷扇贝柔性手爪包络仿真试验平台

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煤岩破碎微小振动监测系统硬件的开发

中州煤炭 2015年第7期 101-104页

作者：孟德龙国投大同能源有限责任公司山西大同037001

在采矿业中,有预见性地监测监控巷道顶板发育,能在很大程度上减少动力灾害带来的损失。设计了基于加速度传感器搭建的监控煤岩破碎微小振动系统,该系统利用加速度传感器接收振动信号,对信号进行放大处理,得到ADC可以转换的模拟信号,并... 详细信息

在采矿业中,有预见性地监测监控巷道顶板发育,能在很大程度上减少动力灾害带来的损失。设计了基于加速度传感器搭建的监控煤岩破碎微小振动系统,该系统利用加速度传感器接收振动信号,对信号进行放大处理,得到ADC可以转换的模拟信号,并转换为数字信号,通过单片机微处理器进行信号处理,用串口通信发送给计算机,最后应用配套编制的软件进行分析。该系统虽处于实验阶段,但其振动监测已经是一个完备过程,且在实际应用中可以更新。

关键词：煤岩破碎振动信号加速度压电传感器数模转换