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Effects of solid-state fermentation product of yeast supplementation on liver and intestinal health,and resistance of common carp(Cyprinus carpio)against spring viraemia carp virus

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Animal Nutrition 2024年第3期18卷 408-418页

作者： mengxin Wang Dongmei Xia Lijuan Yu Qiang Hao Mingxu Xie Qingshuang Zhang Yajie Zhao delong meng Yalin Yang Chao Ran Tsegay Teame Zhen Zhang Zhigang Zhou China-Norway Joint Lab on Fish Gut Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Hubei Key Laboratory of Animal Nutrition and Feed Science Wuhan Polytechnic UniversityWuhan 430000China Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Tigray Agricultural Research Institute Mekelle Agricultural Research CenterMekelleTigray 251Ethiopia

This study aimed to investigate the effects of solid-state fermentation products of yeast(SFPY)on liver and intestinal health and disease resistance of common carp(Cyprinus carpio).A total of 200 common carp with an initial average weight of 2.55±0.004 g were divided into 5 groups(4 replications per group and 10 fish per replication),and were fed with one of five diets,including a control diet and 4 diets supplemented with 2‰(Y2),3‰(Y3),4‰(Y4),or 5‰(Y5)SFPY,respectively,for 8 *** indicated that,the addition of SFPY to the diet of common carp did not affect the growth performance or survival rate of fish(P=0.253).Interestingly,with the addition of SFPY,the triacylglycerol(TAG)content of the liver presented a linear decreasing tendency(P=0.004),with significantly decreased in Y4 and Y5 groups(P=0.035)compared with *** lipopolysaccharide(LPS)content and diamine oxidase(DAO)activity presented a negative linear relationship with the addition of SFPY(P=0.015,P=0.030),while serum lipopolysaccharide binding protein(LBP)content first decreased and then increased(P<0.001).The total antioxidant capacity(T-AOC)in the intestine of fish increased continuously with increasing SFPY supplementation(P=0.026),reaching the highest level in Y5 *** villus height in all experimental groups were significantly higher than that in the control group(P<0.001).Furthermore,compared to the control,adding 3‰SFPY to the control diet of common carp significantly increased the relative abundance of Fusobacteria(P=0.018)and decreased that of Proteobacteria(P=0.039)at phylum level,and increased the relative abundance of Cetobacterium(P=0.018)and decreased that of Shewanella(P=0.013)at genus *** with the control,the relative mRNA expression level of spring viraemia of carp virus N protein(SVCV-n)in the kidney was lower than that of the control group without significance and bottomed out in Y4 group(P=0.138).In conclusion,dietary SFPY enhanced the SVCV resistance capa

关键词： Yeast Solid-state fermentation product Probiotic Immunity Gut microbiota Common carp

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Biocontrol agents modulate phyllosphere microbiota interactions against pathogen Pseudomonas syringae

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Environmental Science and Ecotechnology 2024年第5期21卷 309-317页

作者： Zhaoyue Yang Tianbo Liu Jianqiang Fan Yiqiang Chen Shaolong Wu Jingjing Li Zhenghua Liu Zhendong Yang Liangzhi Li Suoni Liu Hongwu Yang Huaqun Yin delong meng Qianjun Tang College of Plant Protection Hunan Agricultural UniversityChangsha410128HunanChina School of Minerals Processing and Bioengineering Central South UniversityChangsha410083HunanChina Hunan Tobacco Research Institute Changsha410004HunanChina Technology Center Fujian Tobacco Industrial Co.Ltd.Xiamen361000FujianChina School of Architecture and Civil Engineering Chengdu UniversityChengdu610106SichuanChina Yongzhou Tobacco Corporation Yongzhou425000HunanChina

The pathogen Pseudomonas syringae,responsible for a variety of diseases,poses a considerable threat to global crop *** biocontrol strategies employ antagonistic microorganisms,utilizing phyllosphere microecology and systemic resistance to combat this ***,the interactions between phyllosphere microbial dynamics and the activation of the plant defense system remain poorly *** we show significant alterations in phyllosphere microbiota structure and plant gene expression following the application of biocontrol *** reveal enhanced collaboration and integration of Sphingomonas and Methylobacterium within the microbial co-occurrence ***,Sphingomonas inhibits *** by disrupting pathogen chemotaxis and ***,both Sphingomonas and Methylobacterium activate plant defenses by upregulating pathogenesis-related gene expression through abscisic acid,ethylene,jasmonate acid,and salicylic acid signaling *** results highlighted that biocontrol agents promote plant health,from reconstructing beneficial microbial consortia to enhancing plant *** findings enrich our comprehension of the synergistic interplays between phyllosphere microbiota and plant immunity,offering potential enhancements in biocontrol efficacy for crop protection.

关键词： Biological control Metagenome Transcriptome Community structure Pathogen resistance

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Innate endogenous adjuvants prime to desirable immune responses via mucosal routes

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Protein & Cell 2015年第3期6卷 170-184页

作者： Xiaoguang Wang delong meng CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China School of Biomolecular and Biomedical Science University College Dublin Dublin Ireland School of Biology and Environmental Science University College Dublin Dublin Ireland

Vaccination is an effective strategy to prevent infectious or immune related diseases, which has made remarkable contribution in human history. Recently increasing atten- tions have been paid to mucosal vaccination due to its multiple advantages over conventional ways. Subunit or peptide antigens are more reasonable immunogens for mucosal vaccination than live or attenuated pathogens, however adjuvants are required to augment the immune responses. Many mucosal adjuvants have been developed to prime desirable immune responses to different etiologies. Compared with pathogen derived adjuvants, innate endogenous molecules incorporated into mucosal vaccines demonstrate prominent adjuvanticity and safety. Nowadays, cytokines are broadly used as mucosal adjuvants for participation of signal transduction of immune responses, activation of innate immunity and polarization of adaptive immunity. Desired immune responses are promptly and efficaciously primed on basis of specific interactions between cytokines and corresponding receptors. In addition, some other innate molecules are also identified as potent mucosal adjuvants. This review focuses on innate endogenous mucosal adjuvants, hoping to shed light on the development of mucosal vaccines.

关键词： mucosal vaccine adjuvant innate endogenous molecules

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血5′tRF-LysCTT对急性脑梗死早期诊断及病情严重程度判定的价值

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中国急救医学 2024年第8期44卷 733-736,F0003页

作者：苏茜孟德龙王孟可苏志强李国忠哈尔滨医科大学附属第一医院神经内科黑龙江哈尔滨150001

目的探究血5′tRF-LysCTT表达水平对急性脑梗死(ACI)患者早期诊断及病情严重程度判定的价值。方法收集2022年10月至2023年10月在哈尔滨医科大学附属第一医院神经内科住院急性脑梗死(ACI)患者为ACI组(n=114),选取同期健康体检者为对照组(... 详细信息

目的探究血5′tRF-LysCTT表达水平对急性脑梗死(ACI)患者早期诊断及病情严重程度判定的价值。方法收集2022年10月至2023年10月在哈尔滨医科大学附属第一医院神经内科住院急性脑梗死(ACI)患者为ACI组(n=114),选取同期健康体检者为对照组(n=112)。根据NIHSS评分将ACI患者分为轻型组(NIHSS≤5分,n=66)和中重型组(NIHSS>5分,n=48)。通过实时荧光定量聚合酶链反应(RT-qPCR)技术检测两组血5′tRF-LysCTT水平。应用多因素Logistic回归分析预测ACI患者神经功能缺损程度的危险因素。ROC曲线分析5′tRF-LysCTT对区分轻型、中重型ACI的诊断价值。结果ACI患者血5′tRF-LysCTT水平明显低于对照组(1.34±0.56 vs.3.28±1.01,P<0.001)。与轻型组比较,中重型组血5′tRF-LysCTT表达明显降低(1.65±0.45 vs.0.89±0.36,P<0.001)。与5′tRF-LysCTT相对表达量与ACI神经功能缺损程度呈负相关(P<0.001)。ROC曲线分析5′tRF-LysCTT对区分ACI组与对照组的曲线下面积(AUC值)为0.956(95%CI 0.9317~0.9805),敏感度为94.6%,特异度为84.7%,截断值为2.11;区分ACI轻、中重型的AUC值为0.908(95%CI 0.8543~0.9618),敏感度为93.3%,特异度为80.2%,截断值为1.41,具有较高诊断效率。Logistic回归分析显示,5′tRF-LysCTT是预测神经功能缺损程度的独立危险因素。结论血5′tRF-LysCTT可能成为ACI患者早期诊断及病情严重程度判定的新标志物。

关键词：急性脑梗死 tRNA衍生片段 5′tRF-LysCTT 标志物早期诊断

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Effects of redox potential on soil cadmium solubility:Insight into microbial community

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Journal of Environmental Sciences 2019年第1期31卷 224-232页

作者： delong meng Juan Li Tianbo Liu Yongjun Liu Mingli Yan Jin Hu Xiaoqi Li Xueduan Liu Yili Liang Hongwei Liu Huaqun Yin School of Minerals Processing and Bioengineering Central South University Key laboratory of Biometallurgy Ministry of Education College of Agronomy Hunan Agricultural University College of Plant protection Hunan Agricultural University School of Life Science Hunan University of Science and Technology

Understanding the role of microbes in the solubility of cadmium(Cd) is of fundamental importance for remediation of Cd toxicity. The present study aimed to identify the microbes that involved in regulating Cd solubility and to reveal possible mechanisms. Therefore,microbial communities were investigated through high-throughput sequencing approach, the molecular ecological network was constructed and metagenomes were predicted. Our results indicated that redox conditions affected both the solubility of soil Cd and the microbial communities. Anaerobic microbes, such as Anaerolineaceae, did not only play important roles in shaping the microbial community in soils, but might also be involved in regulating the Cd solubility. Two possible mechanisms that how Anaerolineaceae involved in Cd solubility are(1) Anaerolineaceae are important organic matter degraders under anoxic conditions and(2) Anaerolineaceae can co-exist with methane metabolism microbes, while methane metabolism promotes the precipitation of soluble Cd. Thus, application of Anaerolineaceae in bioremediation of soil Cadmium contamination is a potential approach. The study provided a novel insight into the role of microbial community in the regulation of Cd solubility under different redox conditions, and suggested a potential approach for the remediation of soil Cd contamination.

关键词： Cd solubility Redox potential Microbial community Molecular ecology networks (MENs) Anaerolineaceae

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Scene Of Spring--Jiangsu Province China

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ChinAfrica 2021年第4期13卷 4-5页

作者： meng delong(Photograph) 不详

Jiangsu Province China Tourists take a boat tour in Qianduo Scenic Spot in Xinghua,east China’s Jiangsu Province,on March *** Rape Flower Tourism Festival kicked off on the day at the *** can enjoy a vibrant display ... 详细信息

关键词： flowers Jiangsu Spring

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Determining the biological zero for gonadal development razor clams Sinonovacula constricta (lamarck 1818) in Zhejiang,China

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Aquaculture and Fisheries 2021年第1期6卷 56-62页

作者： Baobao Xue delong meng Hao Li Wei Liang Donghong Niu Jiale Li Heding Shen Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics Breeding Shanghai201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China

The biological zero of gonadal development and the effective accumulated temperature of one and two-year-old razor clams Sinonovacula constricta(Lamarck 1818)were studied in Sanmen County,Zhejiang Province,China between April 2016 to October 2017,using a new variety named“Shen-Zhe No.1”.The biological zero was determined by using the rule of effective accumulated temperature and the linear regression method,which was confirmed using a histological analysis of the full gonadal development *** results showed that the biological zero of gonadal reproduction of 1-year-old and 2-year-old *** was the same:20.8℃.When the temperature reached 20–21℃,analysis of the histological sections indicated that more than half of the *** individuals initiated gonadal *** average effective accumulated temperature over two years was 806 d℃.This study contributes to our understanding of the regulation and control of gonadal maturation,suggesting that changes in the spawning and breeding season during one-year of farming using the new variety of razor clam could provide an economic benefit to industrialized large-scale spat *** results demonstrated that the razor clam *** is a typical clam of the accumulative temperature mature oviposition *** addition,although *** does not display hypothermy,it requires a lower temperature for gonadal maturity and spawning.

关键词： Sinonovacula constricta Biological zero Gonads Development Histology

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Leakage Proof,Flame-Retardant,and Electromagnetic Shield Wood Morphology Genetic Composite Phase Change Materials for Solar Thermal Energy Harvesting

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Nano-Micro Letters 2024年第10期16卷 99-120页

作者： Yuhui Chen Yang meng Jiangyu Zhang Yuhui Xie Hua Guo Mukun He Xuetao Shi Yi Mei Xinxin Sheng delong Xie Yunnan Provincial Key Laboratory of Energy Saving in Phosphorus Chemical Engineering and New Phosphorus Materials The International Joint Laboratory for Sustainable Polymers of Yunnan ProvinceThe Higher Educational Key Laboratory for Phosphorus Chemical Engineering of Yunnan ProvinceFaculty of Chemical EngineeringKunming University of Science and TechnologyKunming 650500People's Republic of China Guangdong Provincial Key Laboratory of Functional Soft Condensed Matter School of Materials and EnergyGuangdong University of TechnologyGuangzhou 510006People's Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi'anShaanxi 710072People's Republic of China

Phase change materials(PCMs)offer a promising solution to address the challenges posed by intermittency and fluctuations in solar thermal ***,for organic solid-liquid PCMs,issues such as leakage,low thermal conductivity,lack of efficient solar-thermal media,and flamma-bility have constrained their broad ***,we present an innova-tive class of versatile composite phase change materials(CPCMs)developed through a facile and environmentally friendly synthesis approach,leveraging the inherent anisotropy and unidirectional porosity of wood aerogel(nanowood)to support polyethylene glycol(PEG).The wood modification process involves the incorporation of phytic acid(PA)and MXene hybrid structure through an evaporation-induced assembly method,which could impart non-leaking PEG filling while concurrently facilitating thermal conduction,light absorption,and ***,the as-prepared wood-based CPCMs showcase enhanced thermal conductivity(0.82 W m^(-1)K^(-1),about 4.6 times than PEG)as well as high latent heat of 135.5 kJ kg^(-1)(91.5%encapsula-tion)with thermal durability and stability throughout at least 200 heating and cooling cycles,featuring dramatic solar-thermal conversion efficiency up to 98.58%.In addition,with the synergistic effect of phytic acid and MXene,the flame-retardant performance of the CPCMs has been significantly enhanced,showing a self-extinguishing ***,the excellent electromagnetic shielding of 44.45 dB was endowed to the CPCMs,relieving contemporary health hazards associated with electromagnetic ***,we capitalize on the exquisite wood cell structure with unidirectional transport inherent in the development of multifunctional CPCMs,showcasing the operational principle through a proof-of-concept prototype system.

关键词： Wood PCMs MXene Solar thermal storage and conversion Flame-retardant Electromagnetic shielding

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Synergistic strengthening mechanism of Ca^(2+)-sodium silicate to selective separation of feldspar and quartz

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International Journal of Minerals,Metallurgy and Materials 2024年第9期31卷 1985-1995页

作者： Bo Lin Jingzhong Kuang Yiqiang Yang Zheyu Huang delong Yang Mingming Yu School of Resources and Environmental Engineering Jiangxi University of Science and TechnologyGanzhou 341000China Jiangxi Key Laboratory of Mining Engineering Ganzhou 341000China

Inhibitors are important for flotation separation of quartz and *** this study,a novel combined inhibitor was used to separate quartz and feldspar in near-neutral *** inhibition of the combined inhibitor was assessed by micro-flotation *** a series of detection methods were used to detect differences in the surface properties of feldspars and quartz after flotation reagents and put forward the synergistic strengthening *** outcomes were pointed out that pre-mixing combined inhibitors were more effective than the addition of Ca^(2+)and SS in sequence under the optimal proportion of 1:5.A concentrate from artificial mixed minerals that was characterized by a high quartz grade and a high recovery was acquired,and was found to be 90.70wt% and 83.70%,*** was demonstrated that the combined inhibitor selectively prevented the action of the collector and feldspar from Fourier-transform infrared(FT-IR)and adsorption capacity *** results of X-ray photoelectron spectroscopy(XPS)indicated that Ca^(2+)directly interacts with the surface of quartz to increase the adsorption of *** contrast,the chemistry property of Al on the feldspar surface was altered by combined inhibitor due to Na^(+)and Ca^(2+)taking the place of K^(+),resulting in the composite inhibitor forms a hydrophilic structure,which prevents the adsorption of the collector on the surface of feldspar by interacting with the Al active *** combination of Ca^(2+)and SS synergically strengthens the difference of collecting property between quartz and feldspar by collector,thus achieving the effect of efficient separation.A new strategy for flotation to separate quartz from feldspar in near-neutral pulp was provided.

关键词： feldspar quartz Ca^(2+)-sodium silicate selective adsorption flotation separation

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Spin order and dynamics in the topological rare-earth germanide semimetals

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Science China(Physics,Mechanics & Astronomy) 2024年第10期67卷 113-127页

作者： Yuhao Wang Zhixuan Zhen Jing meng Igor Plokhikh delong Wu Dariusz J.Gawryluk Yang Xu Qingfeng Zhan Ming Shi Ekaterina Pomjakushina Toni Shiroka Tian Shang Key Laboratory of Polar Materials and Devices(MOE) School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200241China Laboratory for Multiscale Materials and Experiments Paul Scherrer InstitutVilligen PSI CH-5232Switzerland Center for Correlated Matter and School of Physics Zhejiang UniversityHangzhou 310058China Laboratory for Muon-Spin Spectroscopy Paul Scherrer InstitutVilligen PSI CH-5232Switzerland Laboratorium für Festkörperphysik ETH ZürichZürich CH-8093Switzerland

The REAl(Si,Ge)(RE=rare earth)family,known to break both the inversion-and time-reversal symmetries,represents one of the most suitable platforms for investigating the interplay between correlated-electron phenomena and topologically nontrivial ***,we report on systematic magnetic,transport,and muon-spin rotation and relaxation(μSR)measurements on(Nd,Sm)AlGe single crystals,which exhibit antiferromagnetic(AFM)transitions at T_(N)=6.1 and 5.9 K,*** addition,NdAlGe undergoes also an incommensurate-to-commensurate ferrimagnetic transition at 4.5 *** transverse-fieldμSR measurements confirm the AFM transitions,featuring a~90%magnetic volume ***-field(ZF)μSR measurements reveal a more disordered internal field distribution in NdAlGe than in SmAlGe,reflected in a larger transverse muon-spin relaxation rateλTat T<

关键词： magneticWeyl semimetals topological Hall effect spin fluctuations μSR

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