检索结果-南通市图书馆

Downregulation of TNFR2 decreases survival gene expression,promotes apoptosis and affects the cell cycle of gastric cancer cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第24期28卷 2689-2704页

作者： Ana Flávia Teixeira Rossi Fernanda da silva Manoel-Caetano Joice Matos Biselli Ágata silva cabral Marilia de Freitas Calmon Saiki Marcelo Lima Ribeiro Ana Elizabete silva Department of Biological Sciences Sao Paulo State University(UNESP)São Josédo Rio Preto 15054-000São PauloBrazil Clinical Pharmacology and Gastroenterology Unit São Francisco University(USF)Bragança Paulista 12916-900São PauloBrazil

BACKGROUND Chronic inflammation due to Helicobacter pylori(***)infection promotes gastric *** necrosis factor-α(TNF-α),a key mediator of inflammation,induces cell survival or apoptosis by binding to two receptors(TNFR1 and TNFR2).TNFR1 can induce both survival and apoptosis,while TNFR2 results only in cell *** dysregulation of these processes may contribute to *** To evaluate the effects of TNFR1 and TNFR2 downregulation in AGS cells treated with *** extract on the TNF-α*** AGS cell lines containing TNFR1 and TNFR2 receptors downregulated by specific shRNAs and nonsilenced AGS cells were treated with *** extract for 6 ***,quantitative polymerase chain reaction with TaqMan®assays was used for the relative quantification of the mRNAs(TNFA,TNFR1,TNFR2,TRADD,TRAF2,CFLIP,NFKB1,NFKB2,CASP8,CASP3)and miRNAs(miR-19a,miR-34a,miR-103a,miR-130a,miR-181c)related to the TNF-αsignalling *** cytometry was employed for cell cycle analysis and apoptosis *** In nonsilenced AGS cells,*** extract treatment increased the expression of genes involved in cell survival and inhibited both apoptosis(NFKB1,NFKB2 and CFLIP)and the TNFR1 ***1 downregulation significantly decreased the expression of the TRADD and CFLIP genes,although no change was observed in the cellular process or miRNA *** contrast,TNFR2 downregulation decreased the expression of the TRADD and TRAF2 genes,which are both important downstream mediators of the TNFR1-mediated pathway,as well as that of the NFKB1 and CFLIP genes,while upregulating the expression of miR-19a and ***,a reduction in the number of cells in the G0/G1 phase and an increase in the number of cells in the S phase were observed,as well as the promotion of early *** Our findings mainly highlight the important role of TNFR2 in the TNF-αpathway in gastric cancer,indicating that silencing it can reduce the

关键词： Gastric cancer Helicobacter pylori Tumour necrosis factor-αsignalling pathway TNFR1 TNFR2 miRNAs

Characterization of soil fauna under the influence of mercury atmospheric deposition in Atlantic Forest, Rio de Janeiro, Brazil

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2015年第6期27卷 217-227页

作者： Andressa Cristhy Buch Maria Elizabeth Fernandes Correia daniel cabral Teixeira Emmanoel Vieira silva-Filho Departamento de Geoquímica Universidade Federal Fluminense Embrapa Agrobiologia BR 465 km 7

The increasing levels of mercury（Hg） found in the atmosphere arising from anthropogenic sources, have been the object of great concern in the past two decades in industrialized countries. Brazil is the seventh country with the highest rate of mercury in the *** major input of Hg to ecosystems is through atmospheric deposition（wet and dry）, being transported in the atmosphere over large distances. The forest biomes are of strong importance in the atmosphere/soil cycling of elemental Hg through foliar uptake and subsequent transference to the soil through litter, playing an important role as sink of this element. Soil microarthropods are keys to understanding the soil ecosystem, and for such purpose were characterized by the soil fauna of two Units of Forest Conservation of the state of the Rio de Janeiro, inwhich one of the areas suffer quite interference from petrochemicals and industrial anthropogenic activities and other area almost exempts of these perturbations. The results showed that soil and litter of the Atlantic Forest in Brazil tend to stock high mercury concentrations, which could affect the abundance and richness of soil fauna, endangering its biodiversity and thereby the functioning of ecosystems.

关键词： Biodiversity Litter Microarthropods Soil Trace-element

Comparative Evaluation of Peptidases Produced by Penicillium corylophilum and Penicillium waksmanii in Solid State Fermentation Using Agro-industrial Residues

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Agricultural Science and Technology(B) 2013年第3期3卷 230-237页

作者： Ronivaldo Rodrigues da silva Tamara Angelo Hamilton cabral Institute of Biosciences Letters and Exact Sciences Sao Paulo State University UNESP SSo Josd do Rio Preto Sao Paulo Brazil Department Pharmaceutical Sciences School of Pharmaceutical Sciences ofRibeirao Preto University ofSao Paulo RibeiraoPreto Sao Paulo CEP 14040-903 Brazil

In this present work, the best conditions for production of peptidases under solid state fermentation by the fungi Penicillium corylophilum and Penicillium waksmanii, partial purification using Sephadex G-75 gel filtration column, as well as the biochemical characterization of the partial purified enzymes were investigated. P. corylophilum showed the best production in medium containing wheat bran, agro-industrial residue, without additives （egg albumin or casein）, in which peptidase activity reached 520 U mL^-1 and the enzyme displayed the optimum activity between pH range from 7 to 8 and 60 ℃. It also showed high stability in wide pH range and temperature until 45 ℃ for 60 min of incubation. On the other hand, P. waksmanii, the best production was noted in a medium containing wheat bran （95%） and casein （5%）, reaching 545 U mL^-1, with proteolytic optimum activity at pH 7.5 and 55 ℃. The enzyme was mainly stable in pH range from 8 to 9 and at temperatures until 45 ℃ for 60 rain of incubation. The peptidases secreted by both fungi were inhibited in the presence of phenylmethane sulfonyl fluoride, showing that they belong to the subclass of serine peptidases.

关键词： Serine peptidase biochemical characterization enzymatic extract fungi.

Ecological implications of twentieth century reforestation programs for the urban forests of São Paulo, Brazil: a study based on litterfall and nutrient cycling

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ecological Processes 2021年第1期10卷 367-379页

作者： Maurício Lamano Ferreira Marcelo Ferreira Barbosa Eduardo Pereira cabral Gomes Ana Paula Branco do Nascimento Edgar Fernando de Luca Karina Gonçalves da silva Ulisses Bezerra França Plínio Barbosa de Camargo Raffaele Lafortezza Master’s Program in Health Promotion Adventist University of São PauloSão PauloSPBrazil. Master’s Program in Geoenvironmental Analysis University of GuarulhosGuarulhosSPBrazil. Department of Health Faculty of Biological ScienceUniversity Nove de Julho(UNINOVE)São PauloSPBrazil. Institute of Botany(IBT/SP) São PauloSPBrazil. Department of Civil Engineering São Judas Tadeu UniversitySão PauloSPBrazil. Institute of Forestry(IF/SP) São PauloSPBrazil. Centro de Energia Nuclear na Agricultura(CENA/USP) University of São PauloPiracicabaSPBrazil. Prefeitura da cidade de São Paulo(Secretaria do Verde e Meio Ambiente) São PauloSPBrazil Department of Agricultural and Environmental Sciences University of Bari Aldo MoroBariItaly. Department of Geography The University of Hong KongCentennial CampusPokfulam RoadHong KongHong Kong.

The focus of this study was to investigate the wide use of Eucalyptus spp.,an exotic plant with high allelopathic ability,in the reforestation programs of urban parks in São Paulo City,Brazil,over the last *** understand the implications of using Eucalyptus *** the city’s parks,this study aimed to compare nutrient cycling and litter decomposition between a mixed composition of litter(i.e.,native and exotic species)and the litter of a single species(i.e.,Eucalyptus).To accomplish this,newly deciduous leaves were collected from two native and two exotic species that are commonly used in the afforestation of São Paulo as well as from Eucalyptus *** mixed composition of litter yielded a higher dry mass loss and return of macro-and micronutrients to the forest *** decomposition constant(k)values were 0.00322 and 0.00207 g g^(−1) day^(−1) for the mixed composition and Eucalyptus spp.,*** time required for decomposition of 50 and 95%of deciduous material was 215 and 931 days,respectively,and for the mixed litterfall 334 and 1449 days,respectively,for Eucalyptus ***,the mixed litter exhibited greater dry mass loss and nutrient cycling in an urban forest of São Paulo City,since dry mass losses as well as speed and amount of nutrients returned to the forest floor were relatively higher compared to Eucalyptus *** cycling via Eucalyptus *** was less efficient than mixed composition of litter,demonstrating that reforestation programs carried out in the twentieth century using only one species may have had little *** results of this work emphasize the fact that in urban reforestation programs the City of São Paulo must consider the environmental and biogeographic characteristics of the species employed and use high levels of biodiversity,since the city lies in a megadiverse biome.

关键词： Tropical forest Exotic species Nutrient dynamics Ecosystem functioning

Productivity and Dry Matter Accumulation of Sugarcane Crop under Irrigation and Nitrogen Application at Rio Verde GO, Brazil

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2015年第14期6卷 2374-2384页

作者： Alefe Viana Souza Bastos Renato Campos de Oliveira Nelmício Furtado da silva Marconi Batista Teixeira Frederico Antonio Loureiro Soares Edson cabral da silva Instituto Federal Goiano (IFgoiano) Rio Verde Brazil

Dry matter production and productivity of stem currently are being widely studied in sugarcane, reinforcing the study in question, which aims to assess the accumulation of dry matter of the aerial segment and the productivity of stems of sugarcane crops within the first cycle, at different levels of water replacement (WR) with and without nitrogen fertilization, through a subsurface drip irrigation system. The assay was conducted in the experimental area of the Federal Institut Goiano, Campus Rio Verde, GO, Brazil, in a dystroferric Rhodic Hapludox soil, cerrado phase (savannah), and comprised experimental splits of three furrows with an 8-meter long double row. Experimental design consisted of randomized blocks in a 5 × 2 factorial array, with four replications. Evaluated factors comprised five levels of WR (100%, 75%, 50%, 25% and 0% of field capacity), with and without the application of nitrogen (0 and 100 kg·ha-1 urea). Harvest occurred in May 2013 and stem productivity (SP), productivity of pointers (PP), productivity of straw (PS), harvest index (HI), dry matter of stem (DMS), dry matter of pointers (DMP), the relationship between dry matter of pointer and dry matter of stem (DMP/DMS) and total dry matter of the aerial segment (TDM) were determined. The variables SP, PP, DMS and DMP had a linear growth in proportion to WR increase, whereas HI and DMP/DMS adjusted to a quadratic model. Nitrogen fertilization affected positively the variables SP, HI, DMS and DMP/DMS and occurred interaction to TDM;also increasing the productivity stem and the harvest index.

关键词： Sugarcane Ratoon Water Replacement Nitrogen Fertilizer

Systemic humoral responses of non-muscle-invasive bladder cancer during BCG treatment: less is more

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cancer Metastasis and Treatment 2017年第1期3卷 116-126页

作者： Fernando M.Calais da silva Paula A.Videira Dário Ligeiro Maria Guadalupe cabral Richard Sylvester Fernando E.Calais da silva Hélder Trindade Grupo Português Génito-Urinário Hospital São José1050-059 LisboaPortugal UCIBIO Departamento Ciências da VidaFaculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal Centro de Estudos de Doenças Crónicas CEDOCNOVA Medical School/Faculdade de Ciências MédicasUniversidade Nova de Lisboa1169-056 LisbonPortugal CDG&Allies Professionals and Patient Associations International Network(CDG&Allies-PPAIN)Faculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal Centro de Sangue e Transplantação de Lisboa IPSTIP.1749-005 LisboaPortugal EAU Guidelines Office 241040 BrusselsBelgium

Aim:Intravesical Bacille Calmette-Guérin(BCG)is the mainstay adjuvant treatment of non-muscle-invasive bladder ***,one third of the patients on BCG regimen relapse within the first year of *** study aimed at identifying biomarkers to predict response to BCG ***:Gene expression was analyzed in blood cells of 58 patients treated with BCG through six consecutive weekly instillations and then at month 3,6,9,and *** tumor necrosis factor(TNF)-α,interleukin(IL)-10,interferon(IFN)-γ,IL-1β,IL-2,IL-4,and IL-6;chemokines CCL2,CCL3,CCL8,CXCL9,and IP-10;and mediators of cytotoxicity CTLA4,Fas-L,Perf,GNLY,NOS2A,and HMOX-1 were analyzed before the 1st and the 6th week instillation and 24 h after to assess fast(within 24 h)and prolonged changes resulting from ***:BCG instillation led to fast-increased expression of IL-1β,TNF-α,and IL-10 *** compared to relapsing patients,patients with no relapses within one year showed significantly lower expression of IL-1βat 1st week and less IFN-γ,HMOX-1,and GNLY at week ***-1 and GNLY were independent predictive biomarkers,and values above the cut-off≥110 and≥13.0‰mRNA,respectively,were considered prejudicial *** with two HMOX-1 and GNLY factors had highest(66.7%)relapsing ***:Assessing immunomodulators’expression in blood allows the establishment of predictive cut-off values and identification of probabilities for patients’relapses after BCG treatment.

关键词： Bladder cancer Bacille Calmette-Guérin immunomodulatory molecules multivariate analysis

Potential role of Limosilactobacillus fermentum as a probiotic with anti-diabetic properties: A review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Diabetes 2022年第9期13卷 717-728页

作者： Diego cabral Lacerda Paulo César Trindade da Costa Paula Brielle Pontes Lucas Alves Carneiro dos Santos JoséPatrocínio Ribeiro Cruz Neto Cristiane Cosmo silva Luis Vanessa Polyana de Sousa Brito JoséLuiz de Brito Alves Department of Nutrition Health Sciences CenterFederal University of ParaíbaJoão Pessoa58051-900ParaíbaBrazil Department of Neuropsychiatry Health Sciences CenterFederal University of PernambucoRecife50670-901PernambucoBrazil Department of Nutrition Federal University of ParaíbaJoão Pessoa58045-190ParaíbaBrazil

Oxidative stress,inflammation,and gut microbiota impairments have been implicated in the development and maintenance of diabetes *** capable of recovering the community of commensal gut microbiota and controlling diabetes mellitus have increased in recent *** lactobacilli strains have an antioxidant and anti-inflammatory system capable of protecting against oxidative stress,inflammation,and diabetes *** studies and some clinical trials have demonstrated that Limosilactobacillus fermentum strains can beneficially modulate the host antioxidant and anti-inflammatory system,resulting in the amelioration of glucose homeostasis in diabetic *** review presents and discusses the currently available studies on the identification of Limosilactobacillus fermentum strains with anti-diabetic properties,their sources,range of dosage,and the intervention time in experiments with animals and clinical *** review strives to serve as a relevant and well-cataloged reference of Limosilactobacillus fermentum strains capable of inducing anti-diabetic effects and promoting health benefits.

关键词： Diabetes Mellitus Gut dysbiosis Oxidative stress Probiotics Limosilactobacillus fermentum

The Quality of Life for People with Parkinson’s Disease in Relation to Their Oral Health

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Stomatology 2018年第11期8卷 312-318页

作者： Phablo Glewber Torres Bezerra Samuel Rodrigo de Andrade Veras Camila Caroline da silva Renato Mariano da silva Deivison Edson Pereira da silva Eduardo Borges da Costa Leite Maria das Gracas Wanderley de Sales Coriolano Carla cabral dos Santos Accioly Lins Federal University of Pernambuco RecifeBrazil Department of Prosthodontics and Oral and Maxillofacial Surgery Federal University of PernambucoRecifeBrazil Department of Anatomy Federal University of PernambucoRecifeBrazil

The present study aimed to evaluate the correlation between the oral health status and the quality of life for people with Parkinson’s disease. The Decayed, Missing, Filled (DMFT) index and the quality of life questionnaire for people with Parkinson’s disease (Parkinson’s Disease Questionnaire-39) were used as evaluation instruments. Sixty-two people with a clinical diagnosis of Parkinson’s disease between stages 1 and 3, age between 46 and 86 years, of both sexes, with an average time of disease evolution of 7 years, participated in the study. data were evaluated using the Pearson’s correlation test, and one-way ANOVA (p daily living.

关键词： Parkinson’s Disease Quality of Life DMFT Index

Vadose Zone Heterogeneity Effect on Unsaturated Water Flow Modeling at Meso-Scale

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Water Resource and Protection 2015年第4期7卷 353-368页

作者： Artur Paiva Coutinho Laurent Lassabatere Thierry Winiarski Jaime Joaquim da silva Pereira cabral Antonio Celso dantas Antonino Rafael Angulo-Jaramillo LEHNA UMR 5023 CNRS ENTPE UCB-Lyon-1 Vaulx-en-Velin France Universidade Federal de Pernambuco Centro de Tecnologia e Geociências Programa de Pós Graduacao em Engenharia Civil/Tecnologia Ambiental e Recursos Hídricos Recife Brasil

The understanding of unsaturated flow in heterogeneous formations is a prerequisite to the understanding of pollutant transfer in the vadose zone and the proper management of infiltration basins settled over such heterogeneous formations. This study addresses the effect of lithological heterogeneity of a glaciofluvial deposit on flow in the vadose zone underneath an infiltration basin settled in the Lyon suburbs. The basin had already been the subject of several previous studies, some of which demonstrated the impact of soil heterogeneity. But all of them were only based on the sedimentological study of a trench and no study addressed the potential spatial variability of results due to the spatial variability of soil heterogeneity. In this study, we model flow in the vadose zone for several case studies, including drainage, water infiltration during a rainfall event, and a complete meteorological chronic. These calculations were conducted for several sections, previously characterized in the basin using GPR and sedimentological study and compared with a blank (homogeneous section). The results clearly show that heterogeneity impacts unsaturated flow and that these impacts depend upon the section considered. Some geometrical architectural and textural parameters were proposed to explain the spatial variability and effect of the soil heterogeneity on unsaturated flow, thus establishing the first step towards modeling unsaturated flow in the basin at the meso-scale.

关键词： Architectural and Structural Indicators Ground Penetration Radar Numerical Modeling Preferential Flow Structural Heterogeneity Unsaturated Flow