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Sensory Phenotypic and molecular Identification of Aromatic Rice Accessions Cultivated in Benin

Advances in Bioscience and Biotechnology 2024年第3期15卷 195-206页

作者： Chimène Nadège mahoussi Nanoukon Koffi David montcho Hambada deless edmond fulgence thiémélé Bignon meyrix Pamela Franzel loumedjinon Babatoundé Franel Carel Wenceslas Affolabi Amed Sèmèvo Havivi Kéllya laurinzo Déguénon Grâce Finagnon Vitoekpon Arielle Bankole lambert Gustave Djedatin laboratory of molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Bio-technologies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin laboratory of molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Biotechnologies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin laboratory of Plant Horticultural and Forestry Sciences National University of Agriculture Kétou Benin Genetics Teaching and Research Unit Department of Biochemistry-Genetics Peleforo GON COULIBALY University Korhogo C&#244te d’Ivoire

Rice is one of the most widely cultivated cereals in the world, and its aroma is increasingly in demand. With the advancement of research, a major rice flavor gene has been identified on rice chromosome 8. It encodes non-functional betaine aldehyde dehydrogenase leading to the accumulation of 2-acetyl-1-pyrroline which is the major olfactory compound that confers the fragrant character to rice. The aroma of rice is considered a special trait of enormous economic importance that determines the prime price in world trade. To satisfy the needs of the population and reduce rice imports into Benin, we conducted this study to identify aromatic rice accessions grown in Benin. Seventy-two rice accessions collected across Benin were PCR amplified with three SSR markers Rm 7049, Aro 7, and Rm 223, linked to the fgr (fragrance of rice) aroma gene. molecular analysis revealed that 12 of the 72 accessions, namely Bagou 19, Bagou 22, Tchaka 34, Foun 15, Tchaka 41, Nana 32, Kan 61, Kung 69, Kung 67, Bagou 20, Agbab 101 and Koum 55 possess the fgr gene and can be considered as aromatic rice accessions. A sensory phenotypic test using KOH was carried out on rice accessions carrying fgr gene. Of the twelve positives, only one had the smell of aromatic rice, like the Azucena control. These results show that Benin also has aromatic rice varieties that can be sold on national and international markets.

关键词： Aromatic Rice 2-Acetyl-1-Pyrroline SSR markers Benin

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Paraclinical, Therapeutic and Evolutionary Profile of Osteoarticular Tuberculosis: A Report about 550 Cases in Abidjan

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Open Journal of Rheumatology and Autoimmune Diseases 2015年第4期5卷 131-136页

作者： mohamed Diomandé fulgence Kaboré Ehaulier Soh Christian louis Kouakou mariam Gbané-Koné Abidou Kawélé Coulibaly Kouassi Jean mermoz Djaha Baly Ouattara edmond Eti Jean Claude Daboiko marcel N’zué Kouakou Department of Rheumatology University Hospital Center of Cocody Abidjan C&ocircte d’Ivoire Department of Rheumatology University Hospital Center of Bouaké Bouaké C&ocircte d’Ivoire

Objective: To describe paraclinical, therapeutic and evolutionary aspects of osteoarticular tuberculosis in Abidjan. materials and method: We realized a descriptive retrospective study carried out in the Rheumatology Department of the University Hospital Center of Cocody in Abidjan about 550 patients over a period of 10 years from January 2004 to December 2013. The paraclinical, therapeutic and evolutionary data had interested us. Results: Osteoarticular tuberculosis represented 12.13% of 4531 rheumatic diseases identified during the study period. The tuberculin skin test was positive in 78.49% of cases. An inflammatory syndrome was present with erythrocyte sedimentation rate and C-reactive protein respectively into 69.45% and 76.54% of cases. The research of mycobacterium tuberculosis was realized into 176 of cases which 50% was positive. Polymerase chain reaction and biopsy of tissue were performed respectively in 8 and 6 cases. Plain radiography (89.82%) and computed tomography (83.63%) were the most realized imaging. Computed tomography has contributed for the diagnostic in 99.78% of cases. The majority of our patients (82.91%) received anti-tuberculous treatment which consisted of two months of combination of rifampicin (R), isoniazid (H), pyrazinamide (Z) and Ethambutol (E) (2RHZE), followed by 10 months of rifampicin (R) and isoniazid (H) (10 RH). The outcome in patients on this treatment was favorable in 528 cases (96%). Conclusion: this study revealed that the research of mycobacterium tuberculosis was often realized and positive. On the contrary, polymerase chain reaction and biopsy of tissue were rarely performed. The main imaging is computed tomography. Our protocol of treatment is effective.

关键词： Osteoarticular Tuberculosis Biology Imaging Abidjan

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