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Neuroscience Bulletin 2020年第6期36卷 598-610页

作者： Penglai Liu Tiantian Cao Jinshan Xu Xingfeng Mao dejuan wang Anan Li Jiangsu Key Laboratory of Brain Disease and Bioinformation Research Center for Biochemistry and Molecular BiologyXuzhou Medical UniversityXuzhou 221004China

The olfactory bulb(OB) is the first relay station in the olfactory *** the OB,mitral/tufted cells(M/Ts),which are the main output neurons,play important roles in the processing and representation of odor *** studies focusing on the function of M/Ts at the single-cell level in awake behaving mice have demonstrated that odor-evoked firing of single M/Ts displays transient/long-term plasticity during ***,we tested whether the neural activity of M/Ts and sniffing patterns are dependent on anticipation and reward in awake behaving *** used an odor discrimination task combined with in vivo electrophysiological recordings in awake,head-fixed mice,and found that,while learning induced plasticity of spikes and beta oscillations during odor sampling,we also found plasticity of spikes,beta oscillation,sniffing pattern,and coherence between sniffing and theta oscillations during the periods of anticipation and/or *** results indicate that the activity of M/Ts plays important roles not only in odor representation but also in salience-related events such as anticipation and reward.

关键词： Olfactory bulb In vivo electrophysiological recording Go/no-go Odor representation

Bioprinted mesenchymal stem cell microfiber-derived extracellular vesicles alleviate unilateral renal ischemia-reperfusion injury and fibrosis by inhibiting tubular epithelial cells ferroptosis

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Bioactive Materials 2024年第10期40卷 649-664页

作者： Qiang Guo Jianwei Chen Jianjian Wu Zijun Mo Lei Ye Wenwen Zhong Yi Zhang Huajian Lai Yifei Zhang Jianguang Qiu Tao Xu dejuan wang Department of Urology The Sixth Affiliated HospitalSun Yat-sen UniversityNo 26 Yuancun Erheng RoadGuangzhou510655China Biomedical Innovation Center The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou510655China Bio-intelligent Manufacturing and Living Matter Bioprinting Center Research Institute of Tsinghua University in ShenzhenTsinghua UniversityShenzhen518057China Department of Research and Development Huaqing Zhimei(Shenzhen)Biotechnology Co.Ltd.ShenzhenGuangdong Province518107China Tsinghua Shenzhen International Graduate School Tsinghua UniversityShenzhen 518055China

Renal unilateral ischemia-reperfusion injury(UIRI)constitutes a significant global health challenge,with poor recovery leading to chronic kidney disease and subsequent renal *** vesicles(EVs)present substantial potential benefits for renal ***,the limited yield and efficacy of EVs produced through traditional methodologies(2D-EVs)severely restrict their widespread ***,the efficient and effective strategies for using EVs in UIRI treatment and their mechanisms remain largely *** this study,we propose an innovative approach by integrating bioprinted mesenchymal stem cell microfiber extracellular vesicles production technology(3D-EVs)with a tail vein injection method,introducing a novel treatment strategy for *** comparison of the biological functions of 2D-EVs and 3D-EVs,both in vitro and in vivo,reveals that 3D-EVs significantly outperform ***,in vitro,3D-EVs demonstrate a superior capacity to enhance the proliferation and migration of NRK-52E cells and mitigate hypoxia/reoxygenation(H/R)-induced injuries by reducing epithelial-mesenchymal transformation,extracellular matrix deposition,and *** vivo,3D-EVs exhibit enhanced therapeutic effects,as evidenced by improved renal function and decreased collagen deposition in UIRI mouse *** further elucidate the mechanism by which 3D-EVs derived from KLF15 ameliorate UIRI-induced tubular epithelial cells(TECs)ferroptosis through the modulation of SLC7A11 and GPX4 *** findings suggest that bioprinted mesenchymal stem cells microfiberderived EVs significantly ameliorate renal UIRI,opening new avenues for effective and efficient EV-based therapies in UIRI treatment.

关键词： Bioprinted 3D-EVs Unilateral renal ischemia-reperfusion injury KLF15 Ferroptosis

Corrigendum to“Synergistically targeting synovium STING pathway for rheumatoid arthritis treatment”[Bioact Mater 24(2022)37–53]

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Bioactive Materials 2024年第10期40卷 665-666页

The authors regret that two images in Fig.3A were originally misplaced by a careless *** corrected versions of these images are provided *** corrections do not alter any findings and conclusions reported in this *** a... 详细信息

关键词： correction finding mistake

Integrative analysis of prognostic long non-coding RNAs with copy number variation in bladder cancer

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第8期22卷 664-681页

作者： Wenwen ZHONG dejuan wang Bing YAO Xiaoxia CHEN Zhongyang wang Hu QU Bo MA Lei YE Jianguang QIU Department of Urology the Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510655China Department of Medical Record Management Section the Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China

Copy number variations(CNVs), which can affect the role of long non-coding RNAs(lncRNAs), are important genetic changes seen in some malignant tumors. We analyzed lncRNAs with CNV to explore the relationship between lncRNAs and prognosis in bladder cancer(BLCA). Messenger RNA(mRNA) expression levels, DNA methylation, and DNA copy number data of 408 BLCA patients were subjected to integrative bioinformatics analysis. Cluster analysis was performed to obtain different subtypes and differently expressed lncRNAs and coding genes. Weighted gene co-expression network analysis(WGCNA) was performed to identify the co-expression gene and lncRNA modules. CNV-associated lncRNA data and their influence on cancer prognosis were assessed with Kaplan-Meier survival curve. Multi-omics integration analysis revealed five prognostic lncRNAs with CNV, namely NR2 F1-AS1, LINC01138, THUMPD3-AS1, LOC101928489, and TMEM147-AS1, and a risk-score signature related to overall survival in BLCA was identified. Moreover, validated results in another independent Gene Expression Omnibus(GEO) dataset, GSE31684, were consistent with these results. Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis revealed that the mitogen-activated protein kinase(MAPK) signaling pathway, focal adhesion pathway, and Janus kinase-signal transducers and activators of transcription(JAK-STAT) signaling pathway were enriched in a high-risk score pattern, suggesting that imbalance in these pathways is closely related to tumor development. We revealed the prognosis-related lncRNAs by analyzing the expression profiles of lncRNAs and CNVs, which can be used as prognostic biomarkers for BLCA.

关键词： Bladder cancer Copy number variation(CNV) Long non-coding RNA(lncRNA) Prognosis

Treatment child kidney caculis by color doppler ultrasound-guided percutaneous nephrostomy

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Treatment child kidney caculis by color doppler ultrasound-g...

Insulin modulates neural activity of pyramidal neurons in the anterior piriform cortex

第十五届全国泌尿外科学术会议

作者： Cai Yubin (1), Gao Xin (2), wang dejuan (3), Lu Minhua (4). 中山大学附属第三医院泌尿外科

Introduction: Urolithiasis in children is a growing *** report our experience with PCNL to treat kidney calculi in children. Methods:A 2.3 years old child was on admission for recurrly urinary tract infection. CTU shows there has a stone in left kidney and no other urinary tract *** stone burden was 10 mm. After to detain a Fr4 ureteral stent in left ureter,child had prone position.

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Insulin modulates neural activity of pyramidal neurons in th...

腹侧被盖区多巴胺能神经元调控LPS处理小鼠的焦虑样行为

中国神经科学学会第十二届全国学术会议

作者： Yang Zhou Tiantian Cao Xiaojie wang Jinshan Xu dejuan wang Diego Restrepo Anan Li Jiangsu Key Laboratory of Brain Disease and Bioinformation Research Center for Biochemistry and Molecular Biology Xuzhou Medical University Department of Cell and Developmental Biology University of Colorado Anschutz Medical Campus

Objective Insulin is an important peptide hormone that regulates food intake and olfactory function. While a multitude of studies investigated the effect of insulin in the olfactory bulb and olfactory epithelium, research on how it modulates higher olfactory centers is lacking. Here we ask how insulin modulates neural activity of pyramidal neurons both in-vitro and in-vivo of the anterior piriform cortex, a key olfactory signal processing center that plays important roles in odor preference learning, odor pattern separation, and perception of odors which may provide some new evidence to the relationship between olfaction and nutritional state. Methods In vitro: Spatial expression of IRs and Ca Mk II alpha（a marker of pyramidal cells） in the APC of the mice were measured by immunostaining. Whole-cell action potential（AP） were recorded in current-clamp mode while miniature-excitatory postsynaptic currents（m EPSCs） and miniature-inhibitatory postsynaptic currents（mIPSCs） were recorded in voltage-clamp mode performing in the brain slices. In vivo:We used fiber photometry to specifically record the calcium signals only from pyramidal neurons and recording the LFP signals from the APC by a single stainless electrode, and changed the insulin level by lateral vertriculus injection. For odor stimulation, awake mice were head-fixed and different odors were presented by an odor delivery system. Insulin level was determined using an insulin enzyme-linked immunosorbent assay（ELISA）kit. Results 1. In total, 92.2%±0.02 of pyramidal neurons were insulin receptor immunopositive, and 53.4%±0.06 of insulin receptor immunopositive cells were pyramidal neurons. 2. Insulin elevated the RMP of most of the neurons and its application significantly decreased the ISI of APs of a substantial proportion of the neurons recorded. Insulin mainly increased the frequencies of m EPSC and decreased mIPSC. 3. Insulin mainly decreased the beta and gamma（both low and high） bands of ongoing LFP signals i

关键词： Anterior pirifrom cortex Insulin Olfaction

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神经解剖学杂志 2022年第5期38卷 485-490页

作者：井思琪仝坤王德娟徐州医科大学生物化学与分子生物学研究中心徐州221004 徐州医科大学江苏省麻醉学重点实验室徐州221004

目的:通过激活腹侧被盖区(VTA)多巴胺能神经元后观察脂多糖(LPS)处理小鼠焦虑样行为的变化,探讨小鼠VTA多巴胺能神经元对LPS处理小鼠焦虑样行为的调控作用。方法:成年雄性C57小鼠随机分为对照组(control)和LPS组,LPS组为按照100μg/kg... 详细信息

目的:通过激活腹侧被盖区(VTA)多巴胺能神经元后观察脂多糖(LPS)处理小鼠焦虑样行为的变化,探讨小鼠VTA多巴胺能神经元对LPS处理小鼠焦虑样行为的调控作用。方法:成年雄性C57小鼠随机分为对照组(control)和LPS组,LPS组为按照100μg/kg腹腔注射脂多糖。1 d后进行旷场实验和高架十字迷宫实验检测小鼠的焦虑情绪。免疫组织荧光染色观察两组小鼠VTA区胶质纤维酸性蛋白(GFAP)和c-Fos表达。成年雄性DAT-cre小鼠随机分为两组,即LPS组与LPS+化学遗传组,LPS造模前3周LPS组双侧VTA注射rAAV-DIO-mCherry病毒;LPS+化学遗传组双侧VTA注射rAAV-DIO-hM3Dq-mCherry病毒,造模1 d后两组小鼠腹腔注射N-氧化氯氮平(CNO),30 min后进行旷场实验和高架十字迷宫实验。结果:旷场实验中,LPS组小鼠进入中央区域的距离与对照组相比明显减少(P0.05)。高架十字迷宫实验中,LPS组小鼠在进入开放臂的探索时间与对照组比明显减少(P<0.05)。与对照组相比,LPS组小鼠脑内VTA区GFAP表达上调,c-Fos表达下降;化学遗传结果显示,与LPS组相比,激活小鼠VTA区多巴胺能神经元后LPS小鼠进入旷场中央区域的距离与进入高架十字迷宫开臂时间明显增加(P<0.05)。结论:LPS处理的小鼠VTA区星形胶质细胞活化增强,表现出焦虑样行为,激活VTA多巴胺能神经元改善了LPS处理小鼠的焦虑样行为。

关键词：脂多糖(LPS) 腹侧被盖区(VTA) 焦虑多巴胺能神经元小鼠

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磁悬浮陀螺转子信号的局部均值分解降噪研究

电子测量与仪器学报 2022年第1期36卷 20-27页

作者：刘迪杨志强王德娟李航长安大学地质工程与测绘学院西安710061

磁悬浮陀螺转子电流信号对环境变化高度敏感,信号采样过程中不可避免会引入噪声,针对该问题提出一种基于局部均值分解(local mean decomposition,LMD),融合豪斯多夫(Hausdorff)距离与阈值降噪(threshold denoising,TD)的算法以减弱噪声... 详细信息

磁悬浮陀螺转子电流信号对环境变化高度敏感,信号采样过程中不可避免会引入噪声,针对该问题提出一种基于局部均值分解(local mean decomposition,LMD),融合豪斯多夫(Hausdorff)距离与阈值降噪(threshold denoising,TD)的算法以减弱噪声干扰。首先对原始信号进行局部均值分解,得到若干乘积函数(PF)分量和一个余量,然后根据各PF分量与原始信号间的豪斯多夫距离判定噪声、信号分量,再对噪声分量进行阈值处理,最后将阈值处理后的噪声分量、信号分量及余量进行叠加得到重构信号,实现陀螺仪转子电流信号的降噪。仿真实验结果表明,重构信号的信噪比相对于原始信号平均提高了12.86 db,均方根误差平均降低了9.25×10^(-6)A;实测信号降噪结果表明,该降噪算法对四条导线边的滤波增益分别为40.0%、93.5%、30.8%和50.0%。

关键词：磁悬浮陀螺仪转子电流信号局部均值分解信号降噪

针刺联合耳穴贴压治疗早中期强直性脊柱炎的疗效观察

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上海针灸杂志 2022年第6期41卷 562-568页

作者：王德娟杨彬河南省中医院郑州450002

目的观察针刺联合耳穴贴压治疗早中期强直性脊柱炎的临床疗效。方法将110例肾虚督寒型早中期强直性脊柱炎患者随机分为对照组(55例)和观察组(55例)。对照组予美洛昔康治疗,观察组采用针刺联合耳穴贴压治疗。观察两组治疗前后疾病活动指... 详细信息

目的观察针刺联合耳穴贴压治疗早中期强直性脊柱炎的临床疗效。方法将110例肾虚督寒型早中期强直性脊柱炎患者随机分为对照组(55例)和观察组(55例)。对照组予美洛昔康治疗,观察组采用针刺联合耳穴贴压治疗。观察两组治疗前后疾病活动指标(晨僵时间、脊柱疼痛、关节肿胀和疲惫感觉)以及脊柱功能(腰椎前屈度、腰椎侧弯度、腰椎后仰度和颈椎旋转度)的变化。观察两组患者血清炎性因子、疼痛介质以及肠道菌群水平的变化。比较两组临床疗效。结果观察组总有效率为98.1%,高于对照组的81.1%(P<0.05)。两组治疗后疾病活动指标和脊柱功能的各项指标均较同组治疗前改善(P<0.05),且观察组治疗后上述各项指标均优于对照组(P<0.05)。观察组治疗后血清炎性因子和疼痛介质各项指标以及肠道肠杆菌和拟杆菌均低于对照组(P<0.05),肠道乳酸杆菌和双歧杆菌高于对照组(P<0.05)。结论针刺联合耳穴贴压可改善肾虚督寒型早中期强直性脊柱炎患者的症状和脊柱功能,降低血清炎性因子和疼痛介质,优化肠道菌群水平,疗效优于口服美洛昔康片治疗。

关键词：针刺疗法耳穴贴压强直性脊柱炎肾虚督寒炎性因子疼痛介质肠道菌群

锦纶6/有机蒙脱土复合纳米纤维的制备与表征

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纺织学报 2007年第11期28卷 1-4页

作者：李琪魏取福汪莹莹王德娟高卫东江南大学生态纺织教育部重点实验室江苏无锡214122

通过高压静电纺丝制备锦纶6/有机蒙脱土复合纳米纤维。采用二步插层复合法对有机蒙脱土进行分散和剥离:先在N,N-二甲基甲酰胺中插层复合,然后在锦纶6溶液中深层复合。应用纳米粒度分析仪分析有机蒙脱土片层相对粒径大小和分布范围;利用... 详细信息

通过高压静电纺丝制备锦纶6/有机蒙脱土复合纳米纤维。采用二步插层复合法对有机蒙脱土进行分散和剥离:先在N,N-二甲基甲酰胺中插层复合,然后在锦纶6溶液中深层复合。应用纳米粒度分析仪分析有机蒙脱土片层相对粒径大小和分布范围;利用扫描电镜观察并分析纳米纤维的直径、外观;运用轻敲模式下的原子力显微镜表征和分析纳米纤维的表面微观结构。结果表明有机蒙脱土加入量对纤维的形态和结构有影响。

关键词：锦纶6 有机蒙脱土复合纳米纤维形态结构