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Journal of Ocean University of China 2024年第1期23卷 216-220页

作者： YU Guihong ZHOU Luning WU Guangwei li dehai Shandong Province Key Laboratory of Applied Mycology and Qingdao International Center on Microbes Utilizing BiogasSchool of Life SciencesQingdao Agricultural UniversityQingdao 266109China School of Medicine and Pharmacy and Sanya Oceanographic InstitutionOcean University of ChinaQingdao 266003China College of Chemical Engineering Nanjing Forestry UniversityNanjing 210037China Laboratory for Marine Drugs and Bioproducts Laoshan LaboratoryQingdao 266237China

Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).

关键词： co-culture Penicillium crustosum Penicillium citrinum dihydropyrones antibacterial activity

Structural diversity and biological activity of natural p-terphenyls

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Marine life Science & Technology 2022年第1期4卷 62-73页

作者： Guoliang Zhou Tianjiao Zhu Qian Che Guojian Zhang dehai li Key Laboratory of Marine Drugs School of Medicine and PharmacyChinese Ministry of EducationOcean University of ChinaQingdao266003China Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao266237China

p-Terphenyls are aromatic compounds consisting of a central benzene ring substituted with two phenyl groups,and they are mainly isolated from terrestrial and marine organisms.The central ring of p-Terphenyls is usually modified into more oxidized forms,e.g.,para quinone and phenols.In some cases,additional ring systems were observed on the terphenyl-type core structure or between two benzene moieties.p-Terphenyls have been reported to have cytotoxic,antimicrobial,antioxidant andα-glucosidase inhibitory effects.In this review,we will mainly summarize the structural diversity and biological activity of naturally occurring p-Terphenyls referring to the research works published before.

关键词： p-Terphenyl Marine organism Structure Biological activity Cytotoxic

Superposition model for analyzing the dynamic ground subsidence in mining area of thick loose layer

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International Journal of Mining Science and Technology 2018年第4期28卷 656-661页

作者： Defeng Hou dehai li Guosheng Xu Yanbin Zhang College of Resources & Safety Engineering China University of Mining & Technology Institute of Energy Science and Engineering Henan Polytechnic Universit

The dynamic ground subsidence due to underground mining is a complicated time-dependent and ratedependent process. Based on the theory of rock rheology and probability integral method, this study developed the superposition model for the prediction and analysis of the ground dynamic subsidence in mining area of thick loose layer. The model consists of two parts(the prediction of overlying bedrock and the prediction of thick loose layer). The overlying bedrock is regarded as visco-elastic beam, of which the dynamic subsidence is predicted by the Kelvin visco-elastic rheological model. The thick loose layer is regarded as random medium and the ground dynamic subsidence is predicted by the probability integral model. At last, the two prediction models are vertically stacked in the same coordinate system, and the bedrock dynamic subsidence is regarded as a variable mining thickness input into the prediction model of ground dynamic subsidence. The prediction results obtained were compared with actual movement and deformation data from Zhao I and Zhao II mine, central China. The agreement of the prediction results with the field measurements show that the superposition model(SM) is more satisfactory and the formulae obtained are more effective than the classical single probability integral model(SPIM), and thus can be effectively used for predicting the ground dynamic subsidence in mining area of thick loose layer.

关键词：动态沉淀重叠模型采矿区地面域分析松积分模型时间依赖

linear polyketides produced by co-culture of Penicillium crustosum and Penicillium fellutanum

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Marine life Science & Technology 2022年第2期4卷 237-244页

作者： Guihong Yu Peng Sun Reyilamu Aierken Chunxiao Sun Zhenzhen Zhang Qian Che Guojian Zhang Tianjiao Zhu Qianqun Gu Mingyu li dehai li Key Laboratory of Marine Drugs Chinese Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao266003China Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao) Qingdao266237China Fujian Provincial Key Laboratory of Innovative Drug Target Research School of Pharmaceutical ScienceXiamen UniversityXiamen361102China School of life Sciences Shandong Province Key Laboratory of Applied MycologyQingdao Agricultural UniversityQingdao266109China

Two new polyketides, penifellutins A (1) and B (2), possessing a 22 carbon linear skeleton, were isolated from a co-culture of the deep-sea-derived fungi Penicillium crustosum PRB-2 and Penicillium fellutanum HDN14-323. Meanwhile, two esterification products of 1, penifellutins C (3) and D (4), were obtained because compound 1 could be esterified spontaneously when stored in methanol. Their configurations were difficult to determine because of chiral central crowdedness, structural flexibility and instability. As such, we solved this issue by comprehensively using Mo2(OAc)4-based CD experiments, density functional theory calculation of 13C NMR, DP4 + probability analysis and many chemical reactions, including making acetonide derivative, Mosher’s method, PGME method, etc. Compounds 1 and 2 show obvious inhibitory activity on the liver hyperplasia of zebrafish larvae at a concentration of 10 μmol/L, while 3 and 4 show no activity, indicating that two carboxyls in the structure are important active sites.

关键词： Co-culture Polyketides Extraction and isolation Structure elucidation Antiproliferation

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蓝莓原浆抗氧化及缓解视疲劳功能研究

中国食品学报 2024年第2期24卷 110-119页

作者：蔡轶男王乐兵李德海东北林业大学生命科学学院哈尔滨150040 黑龙江省森林食品资源利用重点实验室哈尔滨150040

研究蓝莓原浆体内抗氧化及缓解人体视疲劳的功效,分析蓝莓原浆抗氧化功能与改善视疲劳作用的相关性。灌胃给予小鼠低、中、高剂量的蓝莓原浆30 d后,采用试剂盒测定小鼠体内蛋白含量、超氧化物歧化酶(SOD)活力、过氧化氢酶(CAT)活力及丙... 详细信息

研究蓝莓原浆体内抗氧化及缓解人体视疲劳的功效,分析蓝莓原浆抗氧化功能与改善视疲劳作用的相关性。灌胃给予小鼠低、中、高剂量的蓝莓原浆30 d后,采用试剂盒测定小鼠体内蛋白含量、超氧化物歧化酶(SOD)活力、过氧化氢酶(CAT)活力及丙二醛(MDA)含量。选择18~22岁具有视疲劳症状的志愿者共40名,随机分为对照组与试饮组。试饮组每天饮用2瓶蓝莓原浆,即服用量为100 mL/d,连续服用60 d。试验结果显示:与空白对照组相比,摄入0.53 mL蓝莓原浆后,小鼠体内血清SOD活力显著增加至610.76 U/mg prot,同时小鼠体内血清MDA含量显著降低至4.82 nmol/mg prot(P<0.05);摄入0.21 mL蓝莓原浆可显著增加小鼠体内血清CAT酶活力至8.58 U/mg prot(P<0.05)。人体试饮试验表明饮用本蓝莓原浆60 d后,试饮组视疲劳症状及明视持久度均得到良好改善,其中男性群体具有出显著性(P<0.05)。结论:蓝莓原浆具备较好的抗氧化性及缓解人体视疲劳作用。

关键词：蓝莓原浆花青素抗氧化明视持久度视疲劳

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乳酸菌对果蔬产品的抑菌机理及应用

中国食品学报 2024年第2期24卷 432-441页

作者：王璐蒋沙沙李德海孙常雁李书欣东北林业大学生命科学学院哈尔滨150040 哈尔滨理工大学材料科学与化学工程学院哈尔滨150080

随着人们对健康饮食的日益重视,果蔬产品作为重要的营养来源备受关注。然而,果蔬的保藏一直是个难题,传统的果蔬保鲜剂往往依赖于化学物质,其在提高保鲜效果的同时却也带来了潜在的风险和不良影响,因此在果蔬生产和分销链中开发绿色有... 详细信息

随着人们对健康饮食的日益重视,果蔬产品作为重要的营养来源备受关注。然而,果蔬的保藏一直是个难题,传统的果蔬保鲜剂往往依赖于化学物质,其在提高保鲜效果的同时却也带来了潜在的风险和不良影响,因此在果蔬生产和分销链中开发绿色有效的抗菌剂成为研究热点。近年来,利用乳酸菌来延缓果蔬腐败进程已引起研究人员的极大兴趣。乳酸菌可以减缓果蔬腐败,且没有重大不良反应,其作为果蔬防腐剂的应用潜力巨大。然而,乳酸菌的抑菌机理不明确,而且获得监管机构批准并可广泛用于商业用途的乳酸菌或其代谢产物数量仍然很少,导致其在果蔬保鲜贮藏中的应用还不够广泛。本文综述乳酸菌的抑菌机理、应用现状以及在抑制腐败菌方面的优势和局限性,总结近年有关乳酸菌作为果蔬产品抑菌剂的研究进展,并对乳酸菌的发展进行展望,为乳酸菌在果蔬保鲜中的应用提供理论依据。

关键词：果蔬乳酸菌乳酸菌代谢产物抑菌

Immunosuppressant mycophenolic acid biosynthesis employs a new globin-like enzyme for prenyl side chain cleavage

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Acta Pharmaceutica Sinica B 2019年第6期9卷 1253-1258页

作者： Xiwei Chen Lu Wang Jinmei Zhang Tao Jiang Changhua Hu dehai li Yi Zou College of Pharmaceutical Sciences Southwest UniversityChongqing 400715China Key Laboratory of Marine Drugs Chinese Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Biological Science Research Center Southwest UniversityChongqing 400715China

Mycophenolic acid(MPA,1)and its derivatives are first-line immunosuppressants used in organ transplantation and for treating autoimmune diseases.Despite chemical synthetic achievements,the biosynthetic formation of a seven-carbon carboxylic acid pharmacophore side chain of 1,especially the processes involving the cleavage of the prenyl side chain between DHMP(4)and DMMPA(5),remains unknown.In this work,we identified a membrane-bound prenyltransferase,PgMpaA,that transfers FPP to 4 to yield FDHMP(6).Compound 6 undergoes the first cleavage step via a new globin-like enzyme PgMpaB to form a cryptic intermediate 12.Heterologous expression of PgMpa genes in Aspergillus nidulans demonstrates that the second cleavage step(from 12 to 5)of 1 is a PgMpa clusterindependent process in vivo.Our results,especially the discovery of the broad tolerance of substrates recognized by PgMpaB,set up a strategy for the formation of"pseudo-isopentenyl"natural products using fungal globin-like enzymes.

关键词： Biosynthesis Mycophenolic acid Prenylation C-C bond cleavage Globin enzyme

Inhibition of MALT1 paracaspase activity improves lesion recovery following spinal cord injury

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Science Bulletin 2019年第16期64卷 1179-1194页

作者： Hua Zhang Guodong Sun Xiaowei li Zhen Fu Chengbin Guo Guangchao Cao Baocheng Wang Qian Wang Shuxian Yang dehai li Xichun Xia Peng li Jing Zhu Wei Zhou liangyan Zheng Jingxia li Lei Zhang Jianlei Hao libing Zhou Frederic Bornancin Zhizhong li Zhinan Yin Yunfei Gao The First Affiliated Hospital Biomedical Translational Research Institute and School of PharmacyJinan UniversityGuangzhou 510632China Department of Orthopedics First Affiliated HospitalJinan UniversityGuangzhou 510632China Shandong Provincial Qianfoshan Hospital Shandong First Medical UniversityJinan 250014China Guangdong-Hongkong-Macao Institute of CNS Regeneration Ministry of Education CNS Regeneration Collaborative Joint LaboratoryJinan UniversityGuangzhou 510632China Novartis Institutes for BioMedical Research CH-4002 BaselSwitzerland Department of Orthopedics Heyuan People’s Hospital(Heyuan Affiliated Hospital of Jinan University)Heyuan 517199China State Key Laboratory of Biotherapy Collaborative Innovation Center for BiotherapyWest China HospitalSichuan UniversityChengdu 610041China

Spinal cord injury(SCI) is a devastating traumatic injury that causes persistent, severe motor and sensory dysfunction. Immune responses are involved in functional recovery after SCI. Mucosa-associated lymphoid tissue lymphoma translocation 1(MALT1) has been shown to regulate the survival and differentiation of immune cells and to play a critical role in many diseases, but its function in lesion recovery after SCI remains unclear. In this paper, we generated KI(knock in) mice with a point mutation(C472 G) in the active center of MALT1 and found that the KI mice exhibited improved functional recovery after SCI.Fewer macrophages were recruited to the injury site in KI mice and these macrophages differentiated into anti-inflammatory macrophages. Moreover, macrophages from KI mice exhibited reduced phosphorylation of p65, which in turn resulted in decreased SOCS3 expression and increased pSTAT6 levels.Similar results were obtained upon inhibition of MALT1 paracaspase with the small molecule inhibitor‘‘MI-2' or the more specific inhibitor ‘‘MLT-827'. In patients with SCI, peripheral blood mononuclear cells(PBMC) displayed increased MALT1 paracaspase. Human macrophages showed reduced proinflammatory and increased anti-inflammatory characteristics following the inhibition of MALT1 paracaspase. These findings suggest that inhibition of MALT1 paracaspase activity in the clinic may improve lesion recovery in subjects with SCI.

关键词： Anti-inflammatory macrophage Pro-inflammatory macrophage Spinal cord injury NF-j B MALT1 paracaspase activity

物理技术对淀粉结构及OSA酯化淀粉的制备与功能特性影响的研究进展

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现代食品科技 2024年第5期40卷 313-324页

作者：李德海王楠楠杨帆王泽童包怡红王鹏东北林业大学生命科学学院黑龙江哈尔滨150040 黑龙江省森林食品资源利用重点实验室黑龙江哈尔滨150040 华迪集团有限公司北京100195

淀粉资源丰富,应用领域广泛,但是天然淀粉的功能性质具有局限性,制约了其应用,因此淀粉改性研究备受研究学者关注。研究表明辛烯基琥珀酸淀粉酯具有独特的双亲性,改善了淀粉的疏水性和乳化性。然而,传统方法制备的辛烯基琥珀酸淀粉酯效... 详细信息

淀粉资源丰富,应用领域广泛,但是天然淀粉的功能性质具有局限性,制约了其应用,因此淀粉改性研究备受研究学者关注。研究表明辛烯基琥珀酸淀粉酯具有独特的双亲性,改善了淀粉的疏水性和乳化性。然而,传统方法制备的辛烯基琥珀酸淀粉酯效率低,酯化淀粉的功能特性仍不能达到最佳应用要求,因此如何制备高质量的酯化淀粉一直是研究热点。近年来物理技术作为环保、高效的方法被广泛应用于改性淀粉的生产中。研究发现采用物理技术对天然淀粉进行预处理后有利于酯化淀粉的制备,物理技术对淀粉颗粒结构的破坏可以增加淀粉的酯化位点,提高酯化效率。此外,经物理技术预处理后酯化淀粉的功能特性也有所改进,进一步促进淀粉的实际应用。该文对超声波、脉冲电场、超高压、常压等离子体射流、动态高压微流化、球磨等6种物理技术在辛烯基琥珀酸酐酯化淀粉制备中的研究进行总结,综述了6种物理技术的基本原理,并阐述了物理技术对淀粉结构及辛烯基琥珀酸酐酯化淀粉的制备与功能特性的影响。

关键词：淀粉改性物理技术辛烯基琥珀酸酐(OSA) 结构特性

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广播电视工程中数字音频技术的应用

电声技术 2024年第1期48卷 7-9页

作者：况伟杰李德海青岛市城阳区广播电视中心山东青岛266109

为提高广播电视工程的质量与效率,文章以数字音频技术为出发点,分析广播电视工程中数字音频技术的作用,并对数字音频技术在声音采集和编辑、音频编码与解码、数字视频剪辑、声音传输与播放以及音频特技处理等方面的具体应用进行研究,以... 详细信息

为提高广播电视工程的质量与效率,文章以数字音频技术为出发点,分析广播电视工程中数字音频技术的作用,并对数字音频技术在声音采集和编辑、音频编码与解码、数字视频剪辑、声音传输与播放以及音频特技处理等方面的具体应用进行研究,以期为相关从业者提供参考。

关键词：广播电视工程数字音频技术音频编码声音传输