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Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers

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Journal of Integrative Plant Biology 2011年第8期53卷 641-652页

作者： Zhuanfang Hao Xinhai Li Chuanxiao Xie Jianfeng Weng Mingshun Li degui zhang Xiaoling Liang Lingling Liu Sisi Liu Shihuang zhang Institute of Crop Science Chinese Academy of Agricultural Sciences National Key Facilities for Crop Genetic Resources and Improvement Beijing 100081 China Institute of Food Crops Science Xinjiang Academy of Agricultural Sciences Urumqi 830000 China

Single nucleotide polymorphism （SNP） is a common form of genetic variation and popularly exists in maize genome. An Illumina GoldenGate assay with 1 536 SNP markers was used to genotype maize inbred lines and identified the functional genetic variations underlying drought tolerance by association analysis. Across 80 lines, 1 006 polymorphic SNPs （65.5% of the total） in the assay with good call quality were used to estimate the pattern of genetic diversity, population structure, and familial relatedness. The analysis showed the best number of fixed subgroups was six, which was consistent with their original sources and results using only simple sequence repeat markers. Pairwise linkage disequilibrium （LD） and association mapping with phenotypic traits investigated under water-stressed and well-watered regimes showed rapid LD decline within 100–500 kb along the physical distance of each chromosome, and that 29 SNPs were associated with at least two phenotypic traits in one or more environments, which were related to drought-tolerant or drought-responsive genes. These drought-tolerant SNPs could be converted into functional markers and then used for maize improvement by marker-assisted selection.

关键词： association analysis drought tolerance functional variation maize (Zea mays L.) single nucleotide polymorphism.

A differentially methylated region of the ZmCCT10 promoter affects flowering time in hybrid maize

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The Crop Journal 2023年第5期11卷 1380-1389页

作者： Zhiqiang Zhou Xin Lu Chaoshu zhang Mingshun Li Zhuanfang Hao degui zhang Hongjun Yong Jienan Han Xinhai Li Jianfeng Weng Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Flowering time(FT) is a key maize domestication trait, variation in which allows maize to grow in a wide range of latitudes. Although previous studies have investigated the genetic control of FT-related traits per se, few studies of FT hybrid performance have been published. We characterized the genomic architecture associated with hybrid performance for FT in a hybrid panel by testcrossing Chang 7–2 with 328Ye478 × Qi319 recombinant inbred lines(RILs). We identified 11 quantitative trait loci(QTL) for hybrid performance in FT-related traits, including a major QTL qFH10 that controls hybrid performance and heterosis in a summer maize-growing region. However, this locus acts in regulating FT traits per se only in a spring maize-growing region. We validated ZmCCT10 as a candidate gene for qFH10 and found that differences between hybrids and their parental lines in DNA methylation in the differentially methylated region(DMR, –700 to –1520) of the ZmCCT10 promoter affected gene expression pattern and thereby FT in the summer maize-growing region.

关键词： Maize Flowering time Hybrid performance QTL Epigenetic

Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize(Zea mays L.)

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The Crop Journal 2014年第Z1期2卷 132-143页

作者： Liyu Shi Xiangling Lv Jianfeng Weng Hanyong Zhu Changlin Liu Zhuanfang Hao Yu Zhou degui zhang Mingshun Li Xiaoke Ci Xinhai Li Shihuang zhang National Key Facility of Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences College of Agronomy Shenyang Agricultural University Wenshan Academy of Agricultural Sciences

Gray leaf spot(GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.)worldwide,resistance to which is controlled by multiple quantitative trait loci(QTL).To gain insights into the genetic architecture underlying the resistance to this disease,an association mapping population consisting of 161 inbred lines was evaluated for resistance to GLS in a plant pathology nursery at Shenyang in 2010 and ***,a genome-wide association study,using 41,101 single-nucleotide polymorphisms(SNPs),identified 51 SNPs significantly(P<0.001)associated with GLS resistance,which could be converted into 31 *** addition,three candidate genes related to plant defense were identified,including nucleotidebinding-site/leucine-rich repeat,receptor-like kinase genes similar to those involved in basal *** genic SNPs,PZE-103142893 and PZE-109119001,associated with GLS resistance in chromosome bins 3.07 and 9.07,can be used for marker-assisted selection(MAS)of GLS *** results provide an important resource for developing molecular markers closely linked with the target trait,enhancing breeding efficiency.

关键词： Cercospora zeae-maydis Maize SNP Genome-wide association study Candidate genes

Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design

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The Crop Journal 2023年第6期11卷 1884-1892页

作者： Ping Luo Houwen Wang Zhiyong Ni Ruisi Yang Fei Wang Hongjun Yong Lin zhang Zhiqiang Zhou Wei Song Mingshun Li Jie Yang Jianfeng Weng Zhaodong Meng degui zhang Jienan Han Yong Chen Runze zhang Liwei Wang Meng Zhao Wenwei Gao Xiaoyu Chen Wenjie Li Zhuanfang Hao Junjie Fu Xuecai zhang Xinhai Li State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China International Maize and Wheat Improvement Center(CIMMYT) Texcoco 56237Mexico College of Agronomy Xinjiang Agricultural UniversityUrumqi 830091XinjiangChina College of Agronomy Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Institute of Cereal and Oil Crops Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050035HebeiChina Food Crops Research Institute Xinjiang Academy of Agricultural ScienceUrumqi 830091XinjiangChina Maize Research Institute of Shandong Academy of Agricultural Sciences Jinan 250100ShandongChina

Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental *** a GP experiment,34 elite inbred lines were selected to make 285 single-cross hybrids in a partial-diallel cross *** lines represented a mini-core collection of Chinese maize germplasm and comprised 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic *** parents were genotyped by sequencing and the 285 hybrids were phenotyped for nine yield and yield-related traits at two locations in the summer sowing area(SUS)and three locations in the spring sowing area(SPS)in the main maizeproducing regions of *** GP models were employed to assess the accuracy of trait prediction in the *** ten-fold cross-validation,the prediction accuracies of yield performance of the hybrids estimated by the genomic best linear unbiased prediction(GBLUP)model in SUS and SPS were 0.51 and 0.46,*** prediction accuracies of the remaining yield-related traits estimated with GBLUP ranged from 0.49 to 0.86 and from 0.53 to 0.89 in SUS and SPS,*** additive,dominance,epistasis effects,genotype-by-environment interaction,and multi-trait effects were incorporated into the prediction model,the prediction accuracy of hybrid yield performance was *** ratio of training to testing population and size of training population optimal for yield prediction were *** prediction models can improve prediction accuracy in hybrid breeding.

关键词： Maize Genomic prediction Prediction model Genetic effects Hybrid performance

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电视媒介的跨区域、跨媒体经营分析

电视技术 2019年第15期43卷 12-13页

作者：张德贵十堰广播电视台湖北十堰442000

我国城市电视台,受新媒体技术的多种因素牵制,导致经营收入出现断崖式下滑,其固有的节目生产和经营模式不能保障媒体的正常运转。国家提出传统媒体要加强采编发流程再造以及融媒体中心建设的顶层设计,打造新型传媒集团的国家战略需要。... 详细信息

我国城市电视台,受新媒体技术的多种因素牵制,导致经营收入出现断崖式下滑,其固有的节目生产和经营模式不能保障媒体的正常运转。国家提出传统媒体要加强采编发流程再造以及融媒体中心建设的顶层设计,打造新型传媒集团的国家战略需要。基于这一背景,以电视为代表的传统媒体为应对市场纷纷抱团取暖,实现资源整合和媒介融合,打造内容品牌,走跨区域、跨媒体经营之路。

关键词：跨区域跨媒体电视媒体

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X射线荧光光谱法测定铁原料中K、Na、Pb、Zn微量元素

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河北冶金 2013年第4期 22-24页

作者：张德贵河北钢铁集团宣钢公司计量检验中心河北宣化075100

采用高频熔融制备玻璃片样品,用市售国家标样和自制标样绘制工作曲线,确定最佳试验参数,消除干扰因素,成功实现X射线荧光光谱法测定铁原料中K、Na、Pb、Zn等微量元素。该方法检出限低,灵敏度高,操作简单快速,尤其适用于进厂铁原料的批... 详细信息

采用高频熔融制备玻璃片样品,用市售国家标样和自制标样绘制工作曲线,确定最佳试验参数,消除干扰因素,成功实现X射线荧光光谱法测定铁原料中K、Na、Pb、Zn等微量元素。该方法检出限低,灵敏度高,操作简单快速,尤其适用于进厂铁原料的批量检测。

关键词： X射线荧光光谱法铁原料微量元素

CO2气体保护焊在汽车车身焊接中常见缺陷及预防方法

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时代汽车 2021年第18期 139-140页

作者：张德贵上汽通用五菱汽车股份有限公司广西柳州市545007

通过对CO2气体保护焊技术的优劣势和缺陷进行分析,阐述了这些焊接常见设备、在汽车制造工程行业,焊接汽车车身生产过程中可能会产生的一些常见和缺陷问题,并且通过调节,焊接电流、电弧电压、气体流量及外部因素影响的手段和方法,达到有... 详细信息

通过对CO2气体保护焊技术的优劣势和缺陷进行分析,阐述了这些焊接常见设备、在汽车制造工程行业,焊接汽车车身生产过程中可能会产生的一些常见和缺陷问题,并且通过调节,焊接电流、电弧电压、气体流量及外部因素影响的手段和方法,达到有效控制这些缺陷,保证在焊接过程中,每一道焊缝都完美。

关键词：气孔咬边飞溅未焊透烧穿

高中化学创新教育课程开发探索——以上海市徐汇中学“多彩的功能膜”为例

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创新人才教育 2018年第1期 85-89页

作者：张德贵上海市徐汇中学

高中生科技创新素养的培养需要开发更具针对性的校本课程。"多彩的功能膜"是基于化学前沿科技内容开发的高中拓展型课程,其宗旨在于帮助学生接触膜科技前沿领域,经历创新学习过程,培养和发展学生的创新意识和实践能力。该课程包含"准备... 详细信息

高中生科技创新素养的培养需要开发更具针对性的校本课程。"多彩的功能膜"是基于化学前沿科技内容开发的高中拓展型课程,其宗旨在于帮助学生接触膜科技前沿领域,经历创新学习过程,培养和发展学生的创新意识和实践能力。该课程包含"准备创新""体验创新""自主创新"三个阶段,注重发挥学生的主体性并采取社会化评价模式,有效提升了学生的创新素养。

关键词：化学教育创新课程校本课程

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皮带输送机能效优化分析

机械管理开发 2021年第12期36卷 243-244,247页

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为进一步提升带式输送机的稳定、安全、节能运行,在对其能效优化思路研究的基础上,提出从对功率匹配控制的角度对其进行优化控制;其次在优化思路的指导下分别完成带式输送机高效、节能、安全、稳定控制系统的硬件和软件设计;最后,对带式输送机系统的优化节能效果进行验证。

关键词：带式输送机节能变频 PLC控制器软启动功率平衡