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Prevalence and Antibiotic Resistance Pattern among the Mastitis Causing Microorganisms

Open Journal of Veterinary Medicine 2018年第4期8卷 54-64页

作者： Karanvir Singh Mudit Chandra Gurpreet Kaur deepti narang Dhiraj Kumar Gupta Department of Veterinary Microbiology College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana India Department of Veterinary Clinical Medicine College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India

Mastitis is one of the most commonly occurring diseases of dairy animals. It is the most important cause of economic losses to the dairy industry in India and throughout the world. In the present study prevalence of microorganisms isolated from mastitic milk and their antibiotic resistance was studied. A total of sixty nine milk samples from mastitic animals (clinical and subclinical) were tested using sodium lauryl sulphate test and those positive were selected and transported to the laboratory for isolation and identification of the causative agent. Out of these samples fifty samples yielded bacterial growth when tested on selective/non selective medium. Of these fifty samples, seven samples had single bacterial growth whereas rest of the 43 samples had mixed growth. Among the bacterial isolates it was found that the highest prevalence was of Streptococcus agalactiae and Staphylococcus aureus followed by E. coli and Klebsiella pneumoniae. Antibiotic sensitivity test revealed that Streptococcus agalactiae revealed the highest sensitivity to ofloxacin, ciprofloxacin, gentamicin and resistance to amoxicillin and doxycycline;Staphylococcus aureus revealed the highest sensitivity to ciprofloxacin, doxycycline, azithromycin and sparfloxacin and resistance to amoxycillin and gentamicin. Similarly, E. coli revealed the highest sensitivity to azithromycin and chloramphenicol and resistance to amoxicillin and teicoplanin, and Klebsiella pneumoniae revealed highest sensitivity to azithromycin, gentamicin and resistance to amoxicillin, teicoplanin and erythromycin. Thus, from the present study it could be concluded that Streptococcus agalactiae, Staphylococcus aureus, E. coli and Klebsiella pneumoniae were the most frequently isolated organisms from mastitic animals and azithromycin and the third generation fluoroquinolones (ciprofloxacin, sparfloxacin and ofloxacin) were the most sensitive drugs.

关键词： Mastitis Milk Antibiotic Bacteria Resistance

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Diagnosis of Bovine Tuberculosis by Comparative Intradermal Tuberculin Test, Interferon Gamma Assay and <i>esxB</i>(CFP-10) PCR in Blood and Lymph Node Aspirates

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Open Journal of Veterinary Medicine 2019年第5期9卷 55-65页

作者： Derhasar Brahma deepti narang Mudit Chandra Gursimran Filia Amarjit Singh Sikh Tejinder Singh Department of Veterinary Microbiology College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Department of Veterinary Microbiology College of Veterinary ScienceGuru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Animal Disease Research Centre College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Department of Veterinary Pathology College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Directorate Livestock Farm College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences UniversityLudhiana Punjab India

Bovine tuberculosis (TB) is a chronic debilitating disease of huge economic importance due to loss in production, morbidity and mortality, and has a potential zoonotic threat. TB is endemic in India and has a worldwide prevalence, therefore, needing early diagnostic technique for the eradication of TB globally. Currently, compared to the eradication programme of TB in Medical sector, Veterinary sector is lagging behind though TB is one of the major zoonotic diseases prevalent in dairy animals and wildlife in India. With the “End TB” strategy by WHO in human, parallel measures for early diagnosis and culling has to be followed in case of animals for an overall successful eradication programme. The objective of this study is diagnosis of TB in cattle and buffaloes by using the cell-mediated immune response tests, i.e. Comparative Intradermal Tuberculin Test (CITT) and Interferon gamma (IFN-γ) assay, and Polymerase Chain Reaction (PCR) targeting esxB gene (CFP-10 protein) and to compare their diagnostic capabilities. This study was carried out in 202 dairy cattle and buffaloes from an organized dairy farm, where almost all of the animals appeared clinically healthy. We found that, the combined use of both CITT and IFN-γ assay lead to more accurate diagnosis of TB, although IFN-γ assay was more specific than CITT. However, esxB PCR showed almost similar sensitivity to IFN-γ assay and may be used as a fast alternative method for the diagnosis of bovine TB from blood samples.

关键词： Bovine TB CITT IFN-γ esxB PCR

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