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Phytochemical profiling and biological activity of Plectranthus amboinicus(Lour.) mediated by various solvent extracts against Aedes aegypti larvae and toxicity evaluation

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Asian Pacific Journal of Tropical Medicine 2020年第11期13卷 494-502页

作者： deepak paramasivam Balamuralikrishnan Balasubramanian Sungkwon Park Palanisamy Alagappan Tanushri Kaul Wenchao Liu Perumal Pachiappan Department of Biotechnology School of BiosciencesPeriyar UniversitySalemTamil Nadu-636011India Department of Biotechnology Rathnavel Subramaniam College of Arts and ScienceSulur-641402Tamil NaduIndia Department of Food Science and Biotechnology College of Life ScienceSejong UniversitySeoul-05006South Korea Nutritional Improvement of Crops International Centre for Genetic Engineering and BiotechnologyAruna Asaf Ali MargNew Delhi 110067India Department of Animal Science College of AgricultureGuangdong Ocean UniversityZhanjiangGuangdong 524088China Department of Marine Science School of Marine SciencesBharathidasan UniversityTiruchirappalli-620024Tamil NaduIndia

Objective:To analyze the phytochemical compounds and to investigate the bio-toxic efficacy of various solvent extracts of Plectranthus amboinicus against mosquito larvae activity and lethality on non-targeting ***:The methanol,ethyl acetate,hexane,and aqueous extracts of Plectranthus amboinicus were subjected to analyze the mosquitocidal activity against the dengue vector,Aedes aegypti and toxicity assays on zebra fish and brine *** replications were performed,and negative control was also ***,ethyl acetate extract of Plectranthus amboinicus was chosen for the determination of bio-active ***:The mosquitocidal assays of methanol and ethyl acetate extracts of Plectranthus amboinicus showed the maximal activity with minimal concentration against the 4 th instar mosquito-larvae of Aedes aegypti through the following lethal concentration(LC50 and LC90)values:53.36&92.51μg/m L and 13.64&86.09μg/mL,*** addition,the plant extracts showed no toxicity on zebra fish embryo and brine shrimp *** gas-chromatography analysis of the ethyl acetate extract of Plectranthus amboinicus revealed the presence of seven different *** them,PAEA-fraction 60 contained a major active bioactive compound,hexadecanoic acid,methyl ester(270.0).Conclusions:Plectranthus amboinicus possesses mosquitocidal properties and could be used as a potential alternative source for preparing the mosquitocidal agents.

关键词： Plectranthus amboinicus Cold percolation Indian borage Mosquitocidal

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Characterization and screening of in vitro antimalarial and larvicidal activities of selected seaweeds from southeast coast of India against Plasmodium falciparum andAnopheles stephensi

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Journal of Coastal Life Medicine 2017年第6期5卷 242-248页

作者： Rajamani Sowmiya Govindasamy Balasubramani paramasivam deepak Dilipkumar Aiswarya Sundaram Ravikumar SundaramPrasannakumar Pachiappan Perumal Department of Biotechnology School of BiosciencesPeriyar UniversityPeriyar Palkalai NagarSalemTamil Nadu636011India Department of Oceanography and Coastal Area Studies School of Marine SciencesAlagappa UniversityThondi CampusRamanathapuramTamil Nadu623409India

Objective:To investigate the antimalarial and larvicidal activities of seaweeds against Plasmodium falciparum strain andAnopheles stephensi mosquito larva. Methods: The three different seaweeds,Jania rubens,Turbinaria ornata (T. ornata) and Ulva fasciata were collected from Southeast India. The seaweeds were extracted using hexane and ethyl acetate solvents and evaluated for their mosquito-larvicidal and antiplasmodial property. The bioactive compounds of the seaweed extract were studied by Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. Results:The hexane extract ofT. ornata (HTO) has shown significant inhibitory effects at the IC50 value 22.98 μg/mL, when compared to the positive control, chloroquine (12.32 μg/mL). The larvicidal activity of HTO on the fourth instar larvae of Anopheles stephensi was found to be effective with the LC50 and LC90 values (μg/mL) of 1.38 and 3.34. The Fourier transform infrared spectroscopy analysis ofHTO revealed the presence of alkenes, aromatics, amides and amines groups, whereas in gas chromatography-mass spectrometry, 11 different bioactive components were recorded. The bioactive component, hentriacontane in the HTO might be responsible for the recorded antiplasmodial and larvicidal activities. Conclusions:The finding of the present study reveals that the constituents of T. ornata have new sources of antimalarial and larvicidal agents in future.

关键词： Anopheles stephensi FT-IR GC-MS Plasmodium falciparum Turbinaria ornata

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Oxidative stress and female reproductive disorder: A review

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Asian pacific Journal of Reproduction 2022年第3期11卷 107-116页

作者： Kalaivani Manokaran Pavithra Bhat deepak Nayak Ravisankar Baskaran Prabu paramasivam Shiek Fareeth Ahmed Keerthi Priya Karkala Sreedhara Ranganath Pai Vignesh Balaji E Department of Medical Laboratory Technology Manipal College of Health ProfessionsManipal Academy of Higher EducationManipal-576104India Department of Pathology Kasturba Medical CollegeManipal Academy of Higher EducationManipal-576104India Department of Endocrinology Dr ALM PGIBMSTaramaniUniversity of MadrasChennaiIndia School of Medicine Department of NeurologyUniversity of New Mexico Health Sciences CenterUniversity of New MexicoAlbuquerqueNM 87131U.S.A Faculty of Allied Health Science Chettinad Hospital and Research InstituteChennai603103India Department of Pharmacology Manipal College of Pharmaceutical SciencesManipal Academy of Higher EducationManipal-576104India

Oxidative stress arises from an imbalance between the body’s pro-oxidants and ***,oxidative stress has been proven a contributing factor to many female reproductive disorders including infertility,preeclampsia,endometriosis and polycystic ovarian ***,we review the mechanistic role of oxidative stress in inducing the most common female reproductive *** current review has also highlighted the protective role of vitamin C,necessary for certain female reproductive hormone secretion by the antral follicle and corpus luteum and also essential for collagen production in ovarian tissue remodeling after ovulation,in alleviating oxidative stress and thus improving female reproductive outcomes.

关键词： Oxidative stress Gynaecological disorders Infertility Endometriosis Preeclampsia Vitamin C

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