检索结果-南通市图书馆

Transcript shortening via alternative polyadenylation promotes gene expression during fracture healing

Bone Research 2023年第1期11卷 163-177页

作者： deepak kumar Khajuria Irena Nowak Ming Leung Vengadeshprabhu Karuppagounder Yuka Imamura Christopher C.Norbury Fadia Kamal Reyad A.Elbarbary Department of Orthopaedics and Rehabilitation The Pennsylvania State University College of MedicineHersheyPA17033USA Center for Orthopaedic Research and Translational Science(CORTS) The Pennsylvania State University College of MedicineHersheyPA17033USA Institute for Personalized Medicine Penn State College of MedicineHersheyPA17033USA Department of Pharmacology The Pennsylvania State University College of MedicineHersheyPA17033USA Department of Microbiology and Immunology The Pennsylvania State University College of MedicineHersheyPA17033USA Department of Biochemistry and Molecular Biology The Pennsylvania State University College of MedicineHersheyPA17033USA Center for RNA Molecular Biology Pennsylvania State UniversityUniversity ParkPA16802USA

Maturation of the 3′end of almost all eukaryotic messenger RNAs(m RNAs)requires cleavage and *** mammalian m RNAs are polyadenylated at different sites within the last exon,generating alternative polyadenylation(APA)isoforms that have the same coding region but distinct 3′untranslated regions(UTRs).The 3′UTR contains motifs that regulate m RNA metabolism;thus,changing the 3′UTR length via APA can significantly affect gene *** ossification is a central process in bone healing,but the impact of APA on gene expression during this process is ***,we report the widespread occurrence of APA,which impacts multiple pathways that are known to participate in bone ***,the progression of endochondral ossification involves global 3′UTR shortening,which is coupled with an increased abundance of shortened transcripts relative to other transcripts;these results highlight the role of APA in promoting gene expression during endochondral bone *** mechanistic studies of transcripts that undergo APA in the fracture callus revealed an intricate regulatory network in which APA enhances the expression of the collagen,type I,alpha 1(Col1a1)and Col1a2 genes,which encode the 2 subunits of the abundantly expressed protein collagen *** exerts this effect by shortening the 3′UTRs of the Col1a1 and Col1a2 m RNAs,thus removing the binding sites of mi R-29a-3p,which would otherwise strongly promote the degradation of both *** together,our study is the first to characterize the crucial roles of APA in regulating the 3′UTR landscape and modulating gene expression during fracture healing.

关键词： healing metabolism shortening

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multifaceted role of mTOR (mammalian target of rapamycin) signaling pathway in human health and disease

引用

Signal Transduction and Targeted Therapy 2023年第11期8卷 5159-5183页

作者： Vivek Panwar Aishwarya Singh Manini Bhatt Rajiv K.Tonk Shavkatjon Azizov Agha Saquib Raza Shinjinee Sengupta deepak kumar Manoj Garg Department of Pharmaceutical Chemistry School of Pharmaceutical SciencesShoolini UniversitySolanHimachal Pradesh173229India Amity Institute of Molecular Medicine and Stem Cell Research(AIMMSCR) Amity University Uttar PradeshSector-125NoidaUttar Pradesh201313India Department of Biomedical Engineering Indian Institute of TechnologyRoparPunjab140001India Department of Pharmaceutical Chemistry School of Pharmaceutical SciencesDelhi Pharmaceutical Sciences and Research University(DPSRU)New Delhi110017India Laboratory of Biological Active Macromolecular Systems Institute of Bioorganic ChemistryAcademy of Sciences UzbekistanTashkent100125Uzbekistan Faculty of Life Sciences Pharmaceutical Technical University100084TashkentUzbekistan Rajive Gandhi Super Speciality Hospital TahirpurNew Delhi110093India

The mammalian target of rapamycin(mTOR)is a protein kinase that controls cellular metabolism,catabolism,immune responses,autophagy,survival,proliferation,and migration,to maintain cellular *** mTOR signaling cascade consists of two distinct multi-subunit complexes named mTOR complex 1/2(mTORC1/2).mTOR catalyzes the phosphorylation of several critical proteins like AKT,protein kinase C,insulin growth factor receptor(IGF-1R),4E binding protein 1(4E-BP1),ribosomal protein S6 kinase(S6K),transcription factor EB(TFEB),sterol-responsive element-binding proteins(SREBPs),Lipin-1,and Unc-51-like autophagy-activating *** signaling plays a central role in regulating translation,lipid synthesis,nucleotide synthesis,biogenesis of lysosomes,nutrient sensing,and growth factor *** emerging pieces of evidence have revealed that the constitutive activation of the mTOR pathway due to mutations/amplification/deletion in either mTOR and its complexes(mTORC1 and mTORC2)or upstream targets is responsible for aging,neurological diseases,and human ***,we provide the detailed structure of mTOR,its complexes,and the comprehensive role of upstream regulators,as well as downstream effectors of mTOR signaling cascades in the metabolism,biogenesis of biomolecules,immune responses,and ***,we summarize the potential of long noncoding RNAs(lncRNAs)as an important modulator of mTOR ***,we have highlighted the potential of mTOR signaling in aging,neurological disorders,human cancers,cancer stem cells,and drug ***,we discuss the developments for the therapeutic targeting of mTOR signaling with improved anticancer efficacy for the benefit of cancer patients in clinics.

关键词： metabolism homeostasis mTOR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Optimization of Multigrain Premix for High Protein and Dietary Fibre Biscuits Using Response Surface Methodology (RSM)

引用

Food and Nutrition Sciences 2015年第9期6卷 747-756页

作者： Kathalsar Ashwath kumar Gopal kumar Sharma Mohammed Ayub Khan Anil Dutt semwal Cereals and Pulses Technology Division Defence Food Research Laboratory Siddarthanagar Mysore Karnataka India

In order to improve the nutritional quality of biscuits, a multigrain premix (MGP) was developed by using whole barley, sorghum, chickpea, pea and defatted soya flour, each at 20% level. The developed MGP had 26.28% protein, 10.13% insoluble dietary fiber and 7.38% soluble dietary fiber. The experiment was designed to optimise the MGP and wheat flour concentration for the development of multigrain biscuits with high protein, dietary fibre and to maximize the acceptability by the application of central composite rotatable design (CCRD) of Response Surface Methodology (RSM). The levels of incorporation of MGP and wheat flour were taken as variables whereas protein, soluble, insoluble fibers, biscuit dough hardness, breaking strength and overall acceptability (OAA) as responses. The optimum level of MGP and wheat flour obtained using numerical optimization was found to be 40 g and 60 g respectively. The biscuits prepared using these had 16.61% protein, 2.57% soluble fibre, and 6.67% insoluble fibre which is significantly (p ≤ 0.05) higher than control biscuit.

关键词： Multigrain Biscuits Breaking Strength High Protein and Fiber Multigrain Premix Response Surface Methodology (RSM)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparative evaluation of microscopy,OptiMAL® and 18S rRNA gene based multiplex PCR for detection of Plasmodium falciparum & Plasmodium vivax from field isolates of Bikaner,India

引用

Asian Pacific Journal of Tropical Medicine 2013年第5期6卷 346-351页

作者： deepak Pakalapati Shilpi Garg Sheetal Middha Abhishek Kochar Amit kumar Subudhi Boopathi Pon Arunachalam Sanjay kumar Kochar Vishal Saxena Pareek RP Jyoti Acharya Dhanpat kumar Kochar Ashis Das Center for Biotechnology Dept of Biological SciencesBirla Institute of Technology and SciencePilani-333031RajasthanIndia Department of Medicine Sardar Patel Medical CollegeBikaner-334003RajasthanIndia Medical Centre Birla Institute of Technology and SciencePilani-333031RajasthanIndia Kothari Medical and Research Institute BikanerRajasthan-334003India

Objective:To evaluate microscopy,OptiMAL? and multiplex PCR for the identification of Plasmodium falciparum（***） and Plasmodium vivax（***） from the field isolates of Bikaner,Rajasthan（Northwest India）.Methods:In this study,a multiplex PCR（*** and ***） was further developed with the incorporation of Plasmodium malariae（***） specific primer and also a positive *** performance of microscopy,plasmodium lactate dehydrogenase（pLDH） based malaria rapid diagnostic test OptiMAL? and 18S rRNA gene based multiplex PCR for the diagnosis of *** and *** was ***:The three species multiplex PCR ***,*** and ***) with an inbuilt positive control was developed and *** comparison with multiplex PCR,which showed the sensitivity and specificity of 99.36%（95%CI,98.11%-100.00%） and 100.00%（95%CI,100.00%-100.00%）,the sensitivity and specificity of microscopy was 90.44%（95%CI,88.849-95.04%） and 99.22%（95% CI,97.71%-100.00%）,and OptiMAL? was 93.58%（95%CI,89.75%-97.42%） and 97.69%（95%CI, 95.10%-100.00%）.The efficiencies were 99.65%,95.10%and 95.45%for multiplex *** and OptiMAL?.***:Our results raise concerns over the overall sensitivities of microscopy and OptiMAL?,when compared to the multiplex PCR and thus stress the need for new molecular interventions in the accurate detection of the malarial *** further highlights the fact that further developments are needed to improve the performance of rapid diagnostic tests at field level.

关键词： Malaria diagnosis Microscopy OptiMAL® Multiplex PCR 18S rRNA gene Plasmodium

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dosimetric Comparison of Fixed and Flexible Geometry ICRT Applicators in Patients Treated for Carcinoma Cervix

引用

International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2015年第1期4卷 1-6页

作者： Sukhvir Singh Arti Sarin Manoj kumar semwal Ajay kumar Singh Pankaj Goyal Ashok kumar Department of Radiation Oncology Army Hospital (R&R) Delhi Cantonment New Delhi India

In intracavitary radiotherapy (ICRT) for cancer cervix, applicator geometry has the potential to impact the doses to organs at risk (OARs) and the coverage of the target volume. At our centre two Varian made Fletcher-style applicator sets, namely defined (fixed) geometry and flexible geometry, are used for ICRT. In the present work, the two types of applicators were compared dosimetrically as per the recommendations of the International Commission on Radiation Units and Measurements (ICRU-38) while delivering high dose rate brachytherapy. Twenty four patients who underwent ICRT were considered for this study. Radiographic method was used for treatment planning on Abacus planning system. ICRU-38 recommended parameters were estimated and compared. Flexible geometry applicator showed 8.8% and 16% higher bladder and rectum point doses as compared to the fixed geometry one but the difference was statistically not significant. The thickness of the pear-shaped isodose volume was larger for the flexible geometry implant also indicating towards higher doses to OARs. The higher bladder and rectum point doses in the case of flexible applicator will need to be validated by a larger data set.

关键词： Carcinoma Cervix Intracavitary Radiotherapy Applicator Geometry Dosimetry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Emission of Thermal Photon in Heavy-Ion Collisions

引用

Journal of Modern Physics 2014年第9期5卷 792-799页

作者： Poonam Jain Yogesh kumar deepak kumar Department of Physics Shyam Lal College University of Delhi New Delhi India Department of Physics Sri Aurobindo College University of Delhi New Delhi India

We study the leading order processes for photon production using a phenomenological model of quark-gluon plasma (QGP) in relativistic heavy-ion collisions incorporating the parametrization factors in thermal dependent quark mass. The measurement of photon emission provides valuable insights into the early conditions of QGP. The production rate is observed in the low and intermediate range of energy and transverse momentum. The photon yield is found to increase marginally with the effect of thermal dependent quark mass. The results are little enhanced and in good agreement with other work.

关键词： Photons Quark-Gluon Plasma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antioxygenic Activity of <i>Solanum nigrum L.</i>Leaves in Sunflower Oil Model System and Its Thermal Stability

引用

Food and Nutrition Sciences 2014年第11期5卷 1022-1029页

作者： Ananthan Padmashree Gopal kumar Sharma Anil Dutt semwal Chitrashekarachar Mahesh Department of Cereals and Pulses Technology Defence Food Research Laboratory Siddarthanagar Mysore India

The antioxygenic activity of Solanum nigrum L. leaves and its various solvent extracts were evaluated using sunflower oil model system. Leaf powder and its methanol/water (80:20) soluble fraction showed strong antioxygenic activity in refined sunflower oil. On the other hand, ethyl acetate fraction exhibited marginal antioxygenic activity, whereas the water soluble fraction was practically devoid of any activity in refined sunflower oil. Thermal stability of different extracts of Solanum nigrum L. leaves heated at 80?C in refined sunflower oil also indicated the strong efficacy of methanol/water (80:20) extract to inhibit thermal oxidation. Solanum nigrum L. contain high levels of magnesium (239.0 mg/100g) and phosphorous (80.3 mg/100g). Fatty acid analysis of the lipid extracted from Solanum nigrum L. leaves indicated the presence of linoleic (59.1%) as a major fatty acid. The result of this study confirmed the presence of antioxygenic compounds in leaves, in particular its methanol/water (80:20) extracts showed great potential as a natural antioxidant to inhibit lipid peroxidation in foods.

关键词： Solanum nigrum L. Antioxygenic Activity Solvent Extraction Thermal Stability Phenolics Flavonoids

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Optimization of AWJM Process Parameters on 3D-Printed Onyx-Glass Fiber Hybrid Composite

引用

哈尔滨工业大学学报:英文版 2023年第2期30卷 84-98页

作者： Dharmalingam G Arun Prasad M deepak Panghal Sachin Salunkhe Siva kumar M Rathinasuriyan C Department of Mechanical Engineering Vel Tech Rangarajan Dr.Sagunthala R&D Institute of Science and TechnologyAvadiChennai-600062Tamil NaduIndia Department of Fashion Technology National Institute of Fashion TechnologyNew Delhi-11016India

Compared with conventional manufacturing methods, the additive manufacturing technique enhances mechanical properties. The present investigation of additive manufacturing technique is focused on delamination studies of Onyx-Glass Fiber Hybrid Polymer Composite(OGFHPC) along with surface roughness properties during machining(drilling) of composites through abrasive water jet machining(AWJM). However, delamination is a major issue during machining of OGFHPC. In order to overcome these drawbacks, the fabrication of OGFHPC was carried out using fused deposition modeling(FDM) technology which is an additive manufacturing method with Mark forged Mark Two 3D-composite printer. The machinability studies were conducted for drilling operation through AWJM process with different abrasive mass flow rates, drilling diameters and traverse speeds. These drilling machinability process parameters were optimized through the design of experiments-Taguchi analysis followed by the analysis of variance(ANOVA) and validated through the Harmony Search Algorithm(HSA), Whale Optimization Algorithm(WOA) and Grey Wolf Optimization Algorithm(GWO). It was observed that minimum delamination and surface roughness of OGFHPC were optimized for AWJM at abrasive mass flow rates(450 g/min), drilling diameters(12 mm) and traverse speed rate(30 mm/min).

关键词： Onyx-Glass composite fused deposition modeling AWJM delamination surface roughness Taguchi analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Studies on the Optimization and Storage Stability of Virgin Coconut Meal Incorporated Instant Sooji Halwa Mix

引用

Food and Nutrition Sciences 2012年第8期3卷 1092-1099页

作者： Mohammed Ayub Khan Mahesh Chitrashekarchar Anil Dutt semwal Gopal kumar Sharma Defence Food Research Laboratory Siddarthanagar Mysore 570 011 India

Virgin Coconut Meal (VCM) was used for the development of instant wheat sooji (semolina) halwa mix with better nutritional attributes. Central composite rotatable design (CCRD) with 2 independent variables (sugar and VCM) and 4 responses (lightness, redness, taste and overall acceptability) was used for the optimisation. VCM incorporated instant halwa mix prepared using optimised levels of ingredients contained moisture 0.95%;fat 26.2%;protein 7.65%;total ash 0.86%;fibre 1.02% and received overall acceptability score of 8.5 on a 9 point hedonic scale providing 523.86 K·cal/ 100g. The changes in quality of stored VCM incorporated instant halwa mix packed in polypropylene (PP, 75 μ) and laminates of metallised polyester (MP, 90 μ) were monitored in order to assess the shelf-life. Instant halwa mix remained stable and acceptable for one year in both the packaging materials under ambient temperature conditions (15℃ - 34℃). However, the rate of lipid peroxidation was found to be slightly higher in PP packed samples as compared to MP packed ones. Fatty acid composition of VCM incorporated instant halwa mix remained practically unchanged during storage. Oleic acid was the major fatty acid present in fat extracted from halwa mix followed by palmitic and lauric acids.

关键词： Virgin Coconut Meal Optimization Packaging Materials Shelf-Life Overall Acceptability Fatty Acid Composition

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Utilization of Virgin Coconut Meal (VCM) in the Production of Ready-to-Eat Indian Traditional Sweet Meat Using Response Surface Methodology

引用

Food and Nutrition Sciences 2011年第3期2卷 214-221页

作者： Yashi Srivastava Anil Dutt semwal Gopal kumar Sharma Amarinder Singh Bawa 不详

Virgin coconut meal (VCM) was used for the development of ready-to-eat Indian traditional sweet meat commonly known as “ladoo” both with and without sorbic acid (0.3%). The different ingredients were optimized following re-sponse surface methodology with three independent variables i.e. sugar, water and VCM and sensory attributes as responses. The changes in the quality of VCM ladoo packed in polypropylene (PP, 75 μ) and laminates of metalized polyester (MP, 75 μ) were monitored during storage to establish the shelf life under ambient temperature conditions (15?C - 34?C). Samples without added potassium sorbate spoiled within three weeks of storage due to mold growth and fermented odour. VCM ladoo containing sorbic acid did not support any microbial growth during storage up to 4 months, however, during storage samples packed in PP lost moisture and became hard and brittle, while those packed in MP remained soft. Peroxide and thiobarbituric acid values were higher in PP packed samples as compared to those packed in MP. Sorbic acid degraded during storage of ladoo and the rate of degradation was higher in PP packed ladoos than that in MP packed ones.

关键词： Virgin Coconut Meal (VCM) Response Surface Methodology Ladoo Peroxide Value Thiobarbituric Acid Sweet Meat

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：