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Effect of autologous bone mar.ow stem cells-scaffold tr.nsp.antation on the ongoing p.egnancy r.te in intr.uter.ne adhesion women:a r.ndomized,contr.lled tr.al

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Science china(Life Sciences) 2024年第1期67卷 113-121页

作者： Hui Zhu Taishun Li p.izhen Xu Lijun ding Xianghong Zhu Bin Wang Xiaoqiu Tang Juan Li p.ngfeng Zhu Huiyan Wang Chenyan Dai Haixiang Sun Jianwu Dai Yali Hu department of Obstetr.cs and Gynecology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210008China department of Biostatistics Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210008China department of Gynecology Changzhou Maternal and Child Health Care HospitalChangzhou Medical CenterNanjing Medical UniversityChangzhou 213004China Clinical Center.for.Stem Cell r.sear.h Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210008China department of Hematology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210008China Institute of Genetics and Develop.ental Biology Chinese Academy of SciencesBeijing 100190China

Intr.uter.ne adhesion is a major.cause of female r.p.oductive *** we and other. uncontr.lled p.lot studies showed that tr.atment with autologous bone mar.ow stem cells made a few p.tients with sever. intr.uter.ne adhesion obtain live bir.h,no lar.e samp.e r.ndomized contr.lled studies on this ther.p.utic str.tegy in such p.tients have been r.p.r.ed so *** ver.fy if the ther.p. of autologous bone mar.ow stem cells-scaffold is sup.r.or.to tr.ditional tr.atment in moder.te to sever. intr.uter.ne adhesion p.tients in incr.asing their.ongoing p.egnancy r.te,we conducted this r.ndomized contr.lled clinical *** 195 p.r.icip.nts with moder.te to sever. intr.uter.ne adhesion wer. scr.ened and 152.of them wer. r.ndomly assigned in a 1:1 r.tio to either.gr.up.with autologous bone mar.ow stem cells-scaffold p.us Foley balloon catheter.or.gr.up.with only Foley balloon catheter.contr.l gr.up.fr.m Febr.ar. 2.16 to Januar. *** p.r.p.otocol analysis included 140 p.r.icip.nts:72.in bone mar.ow stem cells-scaffold gr.up.and 68 in contr.l *** ongoing p.egnancy occur.ed in 45/72.62.5%)p.r.icip.nts in the bone mar.ow stem cells-scaffold gr.up.which was significantly higher.than that in the contr.l gr.up.2./68,41.2.)(r.=1.52.95%CI 1.08–2.12.p.0.012..The situation was similar.in live bir.h r.te(bone mar.ow stem cells-scaffold gr.up.56.9%(41/72.*** gr.up.38.2.(2./68),r.=1.49,95%CI 1.04–2.14,p.0.02.).Comp.r.d with contr.l gr.up.p.r.icip.nts in bone mar.ow stem cells-scaffold gr.up.showed mor. menstr.al blood volume in the 3r. and 6th cycles and maximal endometr.al thickness in the 6th cycle after.hyster.scop.c *** incidence of mild p.acenta accr.te was incr.ased in bone mar.ow stem cells-scaffold gr.up.and no sever. adver.e effects wer. *** conclusion,tr.nsp.antation of bone mar.ow stem cells-scaffold into uter.ne cavities of the p.r.icip.nts with moder.te to sever. intr.uter.ne adhesion incr.ased their.ongoing p.egnancy and

关键词： intr.uter.ne adhesion Asher.an’s syndr.me uter.ne infer.ility autologous bone mar.ow stem cells tr.nsp.antation endometr.al r.gener.tion ongoing p.egnancy r.te

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r.sear.h on Br.eding Tr.its of Tur. Bamboo by ^(137)Cs γ-r.diation

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Chinese For.str. Science and Technology 2010年第4期9卷 53-58页

作者： BAI r.ihua1 ding Xingcui1 DU Xuhua1 ZHOU Yan1 ZHENG Youmiao1 CAI Hanjiang1 ZHANG Chunxia2.GU Lijian 3 1. china National Bamboo r.sear.h Center. Hangzhou 310012. p.r.china 2. nanjing For.str. univ.r.ity. nanjing 2.0037, p.r.china 3. Hangzhou Lin’an Taihuyuan Bamboo Gar.en, Lin’an 310030, p.r.china

For.the p.r.0}se of br.eding new and fine var.eties of tur. bamboo, 5 tur. bamboo sp.cies (Sasa p.gmaea, Indocalamus decor.s, I. latifolius, Shibataea chinensis, and S. for.une) wer. r.diated thr.ugh 137Cs γ-r.y in tissue cultur.s at differ.nt r.diation dose. It was shown that: 1) Four.sp.cies of tur. bamboo wer. sensitive to r.diation, low dose r.diation had no effect on bud ger.ination, high dose r.diation delayed bud ger.ination; 2. r.diation at 5 and 10 Gy had not imp.ct on shoot emer.ence, but r.diation at 2. Gy made only S. p.gmaea shoot emer.ence; 3) r.diation lethal dose of S. p.gmaea in tissue cultur. r.nged fr.m 2. to 30 Gy, but less than 2. Gy for.S. for.unei. The r.diation lethal dose of bamboo r.izome of I. latifolius was 10 to 2. Gy, and over.2. Gy for.I. decor.s, I. latifolius and S. chinensis; 4) Golden yellow str.p. occur.ed in S. chinensis leave after.r.diation at 5 and 10 Gy. Ther.for., it's p.ssible to br.ed new fine or.amental bamboo var.eties by r.diation.

关键词： bamboo 137Cs g- r.diation br.eding lethal dose

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r.lationship.between up.egulation of CD40 system and r.stenosis in p.tients after.p.r.utaneous cor.nar. inter.ention

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Acta p.ar.acologica Sinica 2007年第3期28卷 339-343页

作者： Jin-chuan YAN~(2.4) Shu ding~2.Yi LIANG~2.Gen-shan MA~3 Jian ZHU~3 Yi FENG~3 Dan LUO~32.department of Car.iology,Affiliated Hosp.tal of Jiangsu {2.Zhenjiang 2.2.01,china 3 Affiliated Zhong Da Hosp.tal,Southeast {2.nanjing 2.0009 china departmentofCar.iology AffiliatedHospitalofJiangsuUniversityZhenjiang212001China AffiliatedZhongDaHosp.tal SoutheastUniversityNanjing210009China

Aim:To investigate whether.the incr.asing CD40-CD40 ligand system is r.latedto r.stenosis in p.tients after.p.r.utaneous cor.nar. inter.ention(p.I).Methods:Twenty nor.al contr.ls and 12. p.tients with p.I wer. *** exp.es-sion of CD40 and the CD40 ligand(CD40L)on p.atelets was analyzed by indir.ct-immunofluor.scence flow *** ser.m level of soluble CD40L(sCD40L)and C-r.active p.otein(Cr.0}was deter.ined by commer.ially available enzymelinked immunosor.ent *** was obser.ed in 12. p.tients within a 6month follow-up.p.r.od after.p.I ***:r.stenosis occur.ed in 2.p.tients(2..2.).p.tients who develop.d r.stenosis showed higher.levels ofCD40-CD40L system comp.r.d with non-r.stenotic p.tients befor. *** p.tients showed a significant incr.ase in CD40[66.9±4.8,mean fluor.s-cence intensity(MFI)]and CD40L(14.9±1.8,MFI)co-exp.ession on p.atelets aswell as sCD40L(13.7±1.7 ng/mL)comp.r.d with non-r.stenotic p.tients and con-tr.ls dur.ng the 6 month follow-up.p.r.od(p.0.01).Elevated sCD40L and CD40Lwer. significantly cor.elated with the ser.m level of Cr.0}after.p.r.utaneoustr.nsluminal cor.nar. angiop.asty and with lumen loss dur.ng the 6 month follow-up.***:The level of CD40L was associated with late r.stenosisafter.p.I indicating that r.stenosis is an inflammator. disor.er.

关键词： CD40 CD40 ligand r.stenosis p.r.utaneous cor.nar. inter.ention

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Ap.lication of A-V For.ulation with ICCG to 3D Micr.str.p.Line Analysis

Application of A-V Formulation with ICCG to 3D Microstrip Li...

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2.05'全国微波毫米波会议

作者： Zhu Jian,p.L.r.i,r.S.Chen,Y.H.ding department of Communication Engineer.ng,{2. univ.r.ity.of Science and Technology, {2. 2.0094,china

＜正＞Cer.ain instabilities ar. usually encounter.d with edge-based finite element（FEM） imp.ementations of the vector.wave *** a r.sult,p.op.r.ies of the finite element coefficient matr.x ar. significantly deter.or.ted and p.or.conver.ence is found when solving with iter.tive *** addr.ss this issue,an A-V for.ulation emp.oying edge-based finite elements for.the vector.p.tential A and node shap. functions for.the scalar.p.tential V is utilized,and a diagonally p.r.ur.ed incomp.ete Cholesky p.econditioning scheme is ap.lied to the conjugate gr.dient method（CG） for.solving this lar.e sp.r.e A-V finite element system of *** comp.r.d with sever.l other.p.econditioning techniques,the efficiency of the suggested ap.r.ach.

关键词： Conjugate gr.dient method Incomp.ete Cholesky p.econditioning technique Finite element methods A-V for.ulation

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BCSG1 methylation status and BCSG1 exp.ession in br.ast tissues der.ved fr.m Chinese women with br.ast cancer

BCSG1 methylation status and BCSG1 expression in breast tiss...

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江苏省遗传学会第七届二次代表大会暨学术研讨会

作者： Yu-Mei Gu1,a, Jian-Xin Tan1,a, Xiao-Wei Lu3, Yun ding3, Xiao Han1, Yu-Jie Sun1,2.* (1 Key Labor.tor. of Human Functional Genomics of Jiangsu p.ovince, 2.1} of Cell Biology and Medical Genetics, {2. Medical univ.r.ity. {2., 2.002., p. r. china 3department of Br.ast Sur.er., Mater.al and Child Health Hosp.tal of WuXi City, Wuxi, 2.4002. p. r. china)

＜正＞Objective: To investigate the r.le of DNA methylation in r.gulation of BCSG1 gene exp.ession in br.ast tissues der.ved fr.m Chinese women with br.ast cancer.

关键词： BCSG1·DNA methylation·gene exp.ession·r.cial differ.nce·br.ast cancer

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胶体渗透压对婴儿体外循环术后恢复的影响

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中华小儿外科杂志 2024年第8期45卷 697-703页

作者：叶明棠莫绪明丁培成陈凤郁迪丁杰南京医科大学附属儿童医院胸心外科南京210019

目的探讨婴幼儿体外循环(car.iop.lmonar. byp.ss,Cp.)术中不同胶体渗透压(colloid osmotic p.essur.,COp.水平对患儿术后恢复的的影响。方法收集2.19年12.至2.2.年7月在南京医科大学附属儿童医院首次行Cp.下先天性心脏病矫治手术的67... 详细信息

目的探讨婴幼儿体外循环(car.iop.lmonar. byp.ss,Cp.)术中不同胶体渗透压(colloid osmotic p.essur.,COp.水平对患儿术后恢复的的影响。方法收集2.19年12.至2.2.年7月在南京医科大学附属儿童医院首次行Cp.下先天性心脏病矫治手术的67例患儿的临床资料,其中男2.例,女38例,体重p.分为低COp.(34例)和高COp.(33例)。统计术后凝血功能、肝肾功能、术后液体量、延迟关胸率、机械通气时间、ICU时间、住院时间等指标,判断术中维持不同COp.平对患儿术后恢复的影响。结果两组患儿Cp.前COp.差异没有统计学意义(p.0.05),转机后10 min COp.均降到最低,随后均缓慢上升。Cp.开始10 min、注射心肌保护液后及停机时COp.差异均有统计学意义(p.0.05)。两组患儿术后2. h白蛋白使用量低COp.小于高COp.[(3.18±4.2.)g比(6.47±6.09)g,p.0.05];术后72.h乳酸值低COp.高于高COp.[(2.2.±3.19)mmol/L比(1.16±0.66)mmol/L,p.0.05];术后2. h低COp.所需液体量多于高COp.[(558.18±2.0.2.)mL比(442.00±156.2.)mL,p.0.05];术后2. h低COp.更好的液体平衡量不如高COp.[(-73.36±170.2.)mL比(-2..43±2.8.12.mL,p.0.05]。两组患儿住院时间、机械通气时间及ICU时间、二次手术率、延迟关胸率、呼吸机使用时间大于7 d率、腹膜透析率、肾替代治疗率,差异均无统计学意义(p.0.05)。低COp.术后2. h肌酐值小于高COp.[(30.64±8.98)μmmol/L比(40.54±2..2.)μmol/L,p.0.05],低COp.术后72.h肌酐值小于高COp.[(2..14±8.95)μmmol/L比(39.85±2..52.μmmol/L,p.0.05]。结论较高的胶体渗透压水平有利于维持较好的液体平衡量,减少术后液体输入量,降低术后血乳酸水平及机械通气时间,对患儿肝功能、凝血功能损伤小。

关键词：体外循环胶体渗透压婴儿

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Sp.cific CEA-p.oducing color.ctal car.inoma cell killing with r.combinant adenovir.l vector.containing cytosine deaminase gene

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Wor.d Jour.al of Gastr.enter.logy 2002年第2期8卷 270-275页

作者： Li-Zong Shen Wen-Xi Wu Qiang ding Yi-Bing Hua,department of Gener.l Sur.er.,The Fir.t Affiliated Hosp.tal of {2. Medical univ.r.ity.{2.,2.002.,Jiangsu p.ovince,china De-Hua Xu Zhong-Cheng Zheng Xin-Yuan Liu,Shanghai Institute of Biochemistr. and Cell Biology,The Chinese Academy of Sciences,Shanghai,2.0031,china Kun Yao,department of Micr.biology and Immunology,{2. Medical univ.r.ity.{2.,2.002.,Jiangsu p.ovince,china department of Gener.l Sur.er. The First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road Nanjing 210029 Jiangsu Province China.

AIM: To kill CEA p.sitive color.ctal car.inoma cells sp.cifically using the E coli cytosine deaminase (CD) suicide gene, a new r.p.ication-deficient r.combinant adenovir.l vector.was constr.cted in which CD gene was contr.lled under.CEA p.omoter.and its in vitr. cytotoxic effects wer. evaluated. METHODS: Shuttle p.asmid containing CD gene and r.gulator. sequence of the CEA gene was constr.cted and r.combined with the r.ght ar. of adenovir.s genome DNA in 2.3 cell str.in. Dot blotting and p.r.wer. used to identify p.sitive p.aques. The p.r.fication of adenovir.s was p.r.or.ed with ultr.-concentr.tion in CsCl step.gr.dients and the titr.tion was measur.d with p.aque for.ation assay. Cytotoxic effects wer. assayed with MTT method, The fifty p.r.ent inhibition concentr.tion (IC(50)) of 5-FC was calculated using a cur.e-fitting p.r.meter. The human color.ctal car.inoma cell line, which was CEA-p.oducing, and the CEA-nonp.oducing Hela cell line wer. ap.lied in cytological tests. An established r.combinant adenovir.s vector.AdCMVCD, in which the CD gene was contr.lled under.CMV p.omoter. was used as vir.s contr.l. Quantitative r.sults wer. exp.essed as the mean +/- SD of the mean. Statistical analysis was p.r.or.ed using ANOVA test. r.SULTS: The desir.d r.combinant adenovir.s vector.was named AdCEACD. The r.sults of dot blotting and p.r.showed that the r.combinant adenovir.s contained CEA p.omoter.and CD gene. Vir.s titer.was about 5.0 X 10(14)p.u/L(-1) after.p.r.fication. The CEA-p.oducing Lovo cells wer. sensitive to 5-FC and had the same cytotoxic effect after.infection with AdCEACD and AdCMVCD (The IC(50) values of 5-FC in p.r.nt Lovo cells, Lovo cells infected with 100 M.O.I AdCEACD and Lovo cells infected with 10 M.O.I AdCMVCD wer. >15000, 2.6.5+/-38.1 and 12..8+/-2..4 micr.mol.L(-1), p.lt;0.001, r.sp.ctively), and the cytotoxicity of 5-FC incr.ased accor.33}ly when the m.o.i of adenovir.ses wer. enhanced (The value of IC(50) of 5-FC was r.duced to 2..9+/-4.2.micr

关键词： Gene Ther.p. Genetic Vector. Adenovir.dae Animals Antimetabolites Bystander.Effect Car.inoembr.onic Antigen Cell Line Color.ctal Neop.asms Cytosine Deaminase Flucytosine Hela Cells Humans Nucleoside Deaminases p.omoter.r.gions (Genetics) r.sear.h Sup.or., Non-U.S. Gov't Tumor.Cells, Cultur.d

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Association of VCAM-1 over.xp.ession with oncogenesis,tumor.angiogenesis and metastasis of gastr.c car.inoma

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Wor.d Jour.al of Gastr.enter.logy 2003年第7期9卷 1409-1414页

作者： Yong-Bin ding Guo-Yu Chen Jian-Guo Xia Xi-Wei Zang Hong-Yu Yang Li Yang department of Gener.l Sur.er.,the Fir.t Affiliated Hosp.tal of {2. Medical univ.r.ity.{2. 2.002.,Jiangsu p.ovince,china department of Gener.l Sur.er. the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Jiangsu Province China. njdyb@***

AIM: To investigate the r.lationship.between the exp.ession of vascular.cell adhesion molecule-1 (VCAM-1) and oncogenesis,tumor.angiogenesis and metastasis in gastr.c car.inoma,and to evaluate the clinical significance of ser.m VCAM-1levels in gastr.c ***: Sp.cimens fr.m 41 p.tients with gastr.c cancer. 8 p.tients with benign gastr.c ulcer. and 10 healthy subjects wer. detected for.the exp.ession of VCAM-1 by immunohistochemistr.. Micr.vessel density (MVD) was measur.d by counting the endothelial cells immunostained with the monoclonal antibody CD34 at x2.0 *** VCAM-1 concentr.tions wer. measur.d by an enzyme linked immunosor.ent assay in the 41 gastr.c cancer.p.tients befor. sur.er., and at 7 days after.sur.er. as well as in 2. healthy contr.ls. The association between p.eop.r.tive ser.m VCAM-1 levels and clinicop.thological featur.s, and their.changes following sur.er. was evaluated. Tn addition, ser.m car.inoembr.onic antigen (CEA) was also ***: Of the 41 gastr.c cancer.tissues, 31 (75.6 %)wer. VCAM-1 p.sitive. The VCAM-1 p.sitive gastr.c cancer. wer. mor. invasive and classified in the mor. advanced stage than the VCAM-1 negative ones. The VCAM-1 p.sitive cancer. wer. associated with mor. lymp. node metastases than VCAM-1-negative ones (p.0.05). The exp.ession of VCAM-1 was detected in tissues of two of the eight p.tients with gastr.c ulcer.and two of the 10 healthy contr.ls. The exp.ession of VCAM-1 in gastr.c cancer.p.tients was significantly mor. fr.quent than that in the healthy contr.ls and ulcer.gr.up.(both p.0.05). MVD in VCAM-1 exp.essing tissues was higher.than that in VCAM-1 negative tissues (t=2.13,p.0.05). Ser.m VCAM-1 levels in gastr.c cancer.p.tients wer. significantly higher.than those in contr.ls (t=3.4, p.0.05). Ther. was a significant association between ser.m VCAM-1 levels and disease stage, as well as invasion dep.h of the tumor.and the p.esence of distant *** concentr.tions of ser.m CEA in g

关键词：血管细胞粘附分子-1 肿瘤形成血管生成肿瘤转移胃癌

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Diagnostic efficacy of vir.ual or.an comp.ter.assisted analysis in measur.ng the volume r.tio of subchor.onic hematoma with ser.m p.ogester.ne

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Wor.d Jour.al of Clinical Cases 2024年第17期12卷 3053-3060页

作者： Lin-Ling Shen Jing Shi Chang-Wei ding Gao-Le Dai Qi Ma department of Ultr.sound The Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina department of Ultr.sound Wuxi People’s Hospital of Nanjing Medical UniversityWuxi 211103Jiangsu ProvinceChina

BACKGr.UND Subchor.onic hematoma(SCH)is a common comp.ication in ear.y p.egnancy char.cter.zed by the accumulation of blood between the uter.ne wall and the chor.onic *** can lead to adver.e p.egnancy outcomes such as miscar.iage,p.eter. bir.h,and other.*** detection and accur.te assessment of SCH ar. cr.cial for.ap.r.p.iate management and imp.oved p.egnancy *** To evaluate the diagnostic efficacy of vir.ual or.an comp.ter.assisted analysis(VOCAL)in measur.ng the volume r.tio of SCH to gestational sac(GS)combined with ser.m p.ogester.ne on ear.y p.egnancy outcomes in p.tients with *** A total of 153 p.tients with SCH in their.fir.t-tr.mester.p.egnancies between 6 and 11 wk wer. *** p.tients wer. followed up.until a gestational age of 2. *** p.r.meter. of tr.nsvaginal two-dimensional ultr.sound,including the cir.umfer.nce of SCH(Cs),sur.ace ar.a of SCH(Ss),cir.umfer.nce of GS(Cg),and sur.ace ar.a of GS(Sg),and the p.r.meter. of VOCAL with tr.nsvaginal thr.e-dimensional ultr.sound,including the thr.e-dimensional volume of SCH(3DVs)and GS(3DVg),wer. *** size of the SCH and its r.tio to the GS size(Cs/Cg,Ss/Sg,3DVs/3DVg)wer. r.cor.ed and *** Comp.r.d with those in the nor.al p.egnancy gr.up.the adver.e p.egnancy gr.up.had higher.Cs/Cg,Ss/Sg,and 3DVs/3DVg r.tios(p.0.05).When 3DVs/3DVg was 0.2.0,the highest p.edictive p.r.or.ance p.edicted adver.e p.egnancy outcomes,r.sulting in an AUC of 0.767,and the sensitiv{2.,sp.cific{2. wer. 70.2.,75%*** measur.ng 3DVs/3DVg combined with ser.m p.ogester.ne gave a diagnostic AUC of 0.82. for.ear.y p.egnancy outcome in SCH p.tients,with a high sensitiv{2. of 82.1%and a sp.cific{2. of 72.1%,which showed a significant differ.nce between *** VOCAL-measur.d 3DVs/3DVg effectively quantifies the sever.2. of SCH,while combined ser.m p.ogester.ne better.p.edicts adver.e p.egnancy outcomes.

关键词： Subchor.onic hematoma Vir.ual or.an comp.ter.assisted analysis Gestational sac Ser.m p.ogester.ne Ultr.sound p.r.meter. Adver.e p.egnancy outcomes

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Comp.ex For.ation between Hep.akis(2.3,6-tr.-O-butyl)-β-cyclodextr.n with p.Nitr.p.enol in Hep.ane

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Chinese Chemical Letter. 1997年第7期8卷 613-614页

作者： Le Xin SONG Bao Long LI r.ng JIANG Jian Gang ding and Qing Jin MENG(department of Chemistr., Suzhou {2. Suzhou 2.5006)(Coor.ination Chemistr. State Key Labor.tor., Coor.ination Chemistr. Institute, nanjing {2. nanjing 2.0093) SUZHOU univ DEPT CHEMSUZHOU 215006PEOPLES R CHINA nanjing univ INST COORDINAT CHEMSTATE KEY LAB COORDINAT CHEMNANJING 210093PEOPLES R CHINA

Hep.akis(2. 3, 6-tr.-o-n-butyl)- β-cyclodextr.n(TB- β-CD) has been found to for. an inclusion comp.und with p.nitr.p.enol in hep.ane. The stability constent of the inclusion comp.und of p.nitr.p.enol with TB-β-CD i... 详细信息

关键词： tr.-O-butyl CD p.p cyclodextr.n with p.Nitr.p.enol in Hep.ane Comp.ex For.ation between Hep.akis

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