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Species-dependent responses of soil microbial properties to fresh leaf inputs in a subtropical forest soil in South China

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journal of Plant Ecology 2014年第1期7卷 86-96页

作者： Faming Wang jin Liu Bi Zou deborah A.Neher Weixing Zhu Zhian Li Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems South China Botanical GardenChinese Academy of SciencesGuangzhou 510160China University of Chinese Academy of Science Beijing 100049China Department of Plant and Soil Science University of VermontBurlington VT 05405USA Department of Biological Sciences State University of New York-BinghamtonBinghamtonNY 13902USA

Aims Forest disturbance from extreme weather events due to climate change could increase the contribution of fresh green leaves to the litter layer of soil and subsequently alter the composition and activity of the soil microbial properties and soil carbon *** objective of this study was to compare the effect of naturally fallen litter and fresh leaves on the soil microbial community composition and their *** Fresh leaves and normal fallen litter were collected from four tree species(Pinus elliottii,Schima superba,Acacia mangium,***)in subtropical China and mixed with *** microbial community composition was determined using PLFAs,and its activity was quantified by soil *** a 12-month period,the decomposition rate of litter was measured bimonthly using a litterbag *** microbial samples were collected after 6 and 12 *** respiration was measured *** Findings We found that fresh leaves decomposed faster than their conspecific fallen *** total microbial biomass and bacterial biomass were similar among treatments,soil fungal biomass was higher in fresh leaf than fallen litter treatments,resulting in greater values of the Fungal phospholipid fatty acids(PLFAs)/Bacterial PLFAs *** PLFA values were greater for Schima superba than the other *** effect of litter type on soil respiration was ***,fallen litter released 35%more CO_(2) than fresh leaves of the conifer *** opposite pattern was observed in the broadleaf species whose fresh leaf treatments emitted 17%–32%more CO_(2) than fallen *** future predictions that global climate change will cause more disturbances to forests,these results indicate that conifer and broadleaf forests in subtropical China may respond differently to increased fresh litter inputs,with net soil microbial respiration decreasing in conifer forests and increasing in broadleaf forests.

关键词： fresh leaf input forest disturbance soil microbial community soil respiration southern China

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短时间与精浆接触对淋巴细胞和巨噬细胞功能和特性的影响

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中国免疫学杂志 1990年第4期6卷 226-230页

作者：张文浩 deborah j.Anderson 天津市计划生育研究所天津300191 美国哈佛医学院Fcaring研究室

通过精浆免疫抑制可逆性实验,精浆对淋巴细胞表面蛋白影响:白细胞趋化运动及巨噬细胞吞噬等试验,研究短时间与人精浆接触对人淋巴细胞和巨噬细胞的生物合成和免疫功能的影响。结果表明人精浆有不可逆影响活化淋巴细胞合成DNA;RNA和蛋白... 详细信息

通过精浆免疫抑制可逆性实验,精浆对淋巴细胞表面蛋白影响:白细胞趋化运动及巨噬细胞吞噬等试验,研究短时间与人精浆接触对人淋巴细胞和巨噬细胞的生物合成和免疫功能的影响。结果表明人精浆有不可逆影响活化淋巴细胞合成DNA;RNA和蛋白的能力,但不影响细胞表面蛋白的表达。提示人精液中的淋巴细胞已受精浆中某些物质所麻痹。然而人精浆并不影响巨噬细胞的吞噬功能,通过人精浆中的某些趋化因子可将巨噬细胞吸引到有精浆的部位。

关键词：精浆淋巴细胞巨噬细胞 AIDS

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Computer Skills in Humanistic Discipline： Some Observations

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journal of Literature and Art Studies 2017年第3期7卷 361-365页

作者： deborah j. Schuster Gerardo M. Acay Middle School Science Boonville R1 Schools Boonville Missouri USA Division of Social Science Missouri Valley College Marshall Missouri USA

This paper explores the myriad capabilities of that contraption, the computer, and its attendant role in the development of creative thought and logical thinking; skills （we could have used the word ＂knowledge＂ instead of skills; but to avoid the unnecessary ambiguity of the former, we shall drop it and talk of skills instead） which the humanistic disciplines and related disciplines in the social sciences and in natural sciences should inculcate. The process of acquiring these skills assumes that one rational animal-the student develops her/his rational powers by studying the wisdom and folly of other rational animals-the authors of various works and/or disciplines the student is set to study. In a word, what is dealt with specifically in the humanistic disciplines, in swimming and riding bicycles, leave us only when decrepitude invades our minds and bodies. In short, students must learn to think. This is an obvious platitude.

关键词： computer skills critical/logical thinking humanistic rational

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Elucidating the impact of the ionomer equivalent weight on a platinum group metal-free PEMFC cathode via oxygen limiting current

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SusMat 2023年第1期3卷 72-90页

作者： HaoWang Luigi Osmieri Haoran Yu Michael j.Zachman jae Hyung Park Nancy N.Kariuki Firat C.Cetinbas Sunilkumar Khandavalli Scott Mauger deborah j.Myers David A.Cullen Kenneth C.Neyerlin Chemistry and Nanoscience Center National Renewable Energy LaboratoryGoldenColoradoUSA Center for Nanophase Materials Sciences Oak Ridge National LaboratoryOak RidgeTennesseeUSA Chemical Sciences and Engineering Division Argonne National LaboratoryLemontIllinoisUSA Nuclear Science and Engineering Division Argonne National LaboratoryLemontIllinoisUSA

Leveraging the interactions between ionomer and catalyst can increase the performance of proton exchangemembrane fuel *** impacts of the equivalent weight(EW)of perfluorosulfonic acid-based ionomers on the platinum group metal-free electrode structure and fuel cell performance have not been fully *** membrane electrode assemblies(MEAs)were prepared by using a commercial Fe-N-C catalyst,two perfluorosulfonic acid ionomers with different EWs,that is,Aquivion 720(A720)and Nafion 1100(N1100),and two ionomer-tocatalyst(I/C)*** four MEAswere characterized to understand the impact of the ionomer EW and content on the capacitance,proton conductivity,and mass transport on the *** mass transport resistance was measured for the first time using a new oxygen reduction reaction limiting current method enabling to couple the effects of oxygen diffusion with liquid water *** EW ionomer combined with a moderate I/C results in improved performance due to its enhanced proton ***,when used at high I/C,it can cause severewater flooding at high current density due to the enhanced liquid water uptake,especially at high relative humidity,resulting in lower catalyst utilization and higher mass transport resistance.

关键词： ionomer equivalent weight limiting current mass transport oxygen reduction reaction PGMfree catalyst proton exchange membrane fuel cell

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Regulation of Wnt/β-catenin signaling by herpesviruses

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World journal of Virology 2016年第4期5卷 144-154页

作者： Kevin j Zwezdaryk joseph A Combs Cindy A Morris deborah E Sullivan Department of Microbiology and Immu-nology Tulane University School of Medicine

The Wnt/β-catenin signaling pathway is instrumental in successful differentiation and proliferation of mammalian cells. It is therefore not surprising that the herpesvirus family has developed mechanisms to interact with and manipulate this pathway. Successful coexistence with the host requires that herpesviruses establish a lifelong infection that includes periods of latency and reactivation or persistence. Many herpesviruses establish latency in progenitor cells and viral reactivation is linked to host-cell proliferation and differentiation status. Importantly, Wnt/β-catenin is tightly connected to stem/progenitor cell maintenance and differentiation. Numerous studies have linked Wnt/β-catenin signaling to a variety of cancers, emphasizing the importance of Wnt/β-catenin pathways in development, tissue homeostasis and disease. This review details how the alpha-, beta-, and gammaherpesviruses interact and manipulate the Wnt/β-catenin pathway to promote a virus-centric agenda.

关键词： Herpesvirus Herpes simplex virus-1 Varicella zoster virus Cytomegalovirus Epstein-Barr virus Kaposi’s sarcoma-associated herpesvirus Wnt/β-catenin Glycogen synthase kinase-3 Axin

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Vegetative propagation of Apuleia leiocarpa(Vogel)***

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journal of Forestry Research 2022年第2期33卷 455-462页

作者： joséGabriel de Souza Silva Diêgo Faustolo Alves Bispo David Pessanha Siqueira Giovanna Campos Mamede Weiss de Carvalho deborah Guerra Barroso Laboratório de Fitotecnia Universidade Estadual do Norte Fluminense Darcy RibeiroAvenida Alberto LamegoCampos dos GoytacazesRJ 2000Brazil

Apuleia leiocarpa is an environmentally and economically significant Atlantic Forest species threat-ened by ongoing *** vegetative propagation of the species could be a step forward to enable implanta-tion of clonal seed orchards and multiplication of selected trees but remains unknown to *** study assessed the mini-cutting technique for propagation of *** and determined the time required for rooting and viable mini-cutting type and *** results show that it is possible to propagate *** with mini-cuttings derived from mini-stumps produced with seeds;the mini-stumps pre-sented regrowth capacity and remained constantly produc-tive for 1 year;10-cm *** mini-cuttings should remain under misting conditions for at least 40 days,and the vegetative propagation of *** with intermediate 7-to 10-cm mini-cuttings resulted in more vigorous clonal seedlings than smaller mini-cuttings.

关键词： Mini-cutting Tropical woody species Adventitious rooting Fabaceae

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婴儿广泛瘙痒性皮疹一例

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英国医学杂志中文版 2021年第8期24卷 479-480页

作者： Michael j Lavery deborah Woodcock Will Simmons Ali Al-Sharqi 秦思(译) Department of Dermatology Alder Hey Children′s Hospital NHS Foundation TrustLiverpoolUK Department of Histopathology Alder Hey Children′s Hospital NHS Foundation TrustLiverpoolUK 广东省第二人民医院皮肤科

1名7月龄的婴儿因全身广泛瘙痒性皮疹3个月就诊于皮肤科门诊,皮疹主要分布在躯干和四肢。家庭医生予以患儿外用糖皮质激素和口服抗生素治疗,未见明显好转。患儿一般情况良好。体格检查:全身弥漫分布多形性皮疹,伴有红斑丘疹、结节、粘... 详细信息

1名7月龄的婴儿因全身广泛瘙痒性皮疹3个月就诊于皮肤科门诊,皮疹主要分布在躯干和四肢。家庭医生予以患儿外用糖皮质激素和口服抗生素治疗,未见明显好转。患儿一般情况良好。体格检查:全身弥漫分布多形性皮疹,伴有红斑丘疹、结节、粘连斑块(图1)。掌指关节和指间隙有结痂丘疹。双足底可见匐行隧道,皮肤镜下可见数个黑点。面部也有受累,左侧眶周和左侧鼻部可见皮疹。

关键词：掌指关节家庭医生红斑丘疹瘙痒性皮疹多形性皮疹皮肤镜眶周皮肤科门诊

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Isolation of Cell Lines for HPV Life Cycle Studies

Isolation of Cell Lines for HPV Life Cycle Studies

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24th International Papillomavirus Conference and Clinical Workshop

作者： Erin Isaacson deborah j jackson Ken Raj john Doorbar Division of Virology National Institute for Medical ResearchMill Hill.LondonUnited Kingdom Division of Virology National Institute for Medical ResearchMill Hill.LondonUnited Kingdom Division of Virology National Institute for Medical ResearchMill Hill.LondonUnited Kingdom Division of Virology National Institute for Medical ResearchMill Hill.LondonUnited Kingdom

＜正＞The HPV productive cycle requires the maintenance of viral episomes in cells of the basal layer,and the initiation of viral genome amplification and the assembly of infectious virions in the upper epithelial *** events can be recreated in the laboratory using raft culture techniques,using keratinocytes transfected with viral episomes,or keratinocyte cell lines derived from low-grade cervical *** two such lines are in common use for the analysis of HPV life-cycle events,the W12 line,

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人血小板在WT小鼠和SCID小鼠体内复苏率的比较

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中国输血杂志 2009年第7期22卷 539-542页

作者：聂咏梅 deborah F.Dumont Larry j.Dumont 广州血液中心广东广州510095 Dartmouth-Hitchcock Medical Center

目的观察人血小板(hPLTs)输注给WT小鼠和SCID小鼠后,在2种小鼠体内的复苏情况。方法通过一侧尾静脉分别给11只WT(FVB/Nj)小鼠和10只SCID小鼠输注单采的hPLTs 200μl/只,在输注后5、60、120、240 min从WT和SCID小鼠对侧尾静脉,以及360 mi... 详细信息

目的观察人血小板(hPLTs)输注给WT小鼠和SCID小鼠后,在2种小鼠体内的复苏情况。方法通过一侧尾静脉分别给11只WT(FVB/Nj)小鼠和10只SCID小鼠输注单采的hPLTs 200μl/只,在输注后5、60、120、240 min从WT和SCID小鼠对侧尾静脉,以及360 min从SCID小鼠对侧尾静脉采集小鼠全血10μl/只(次),用3.8%EDTA抗凝后,立即采用4℃保存的1%多聚甲醛固定,4℃过夜保存;将固定的小鼠全血样本经过洗涤和再悬浮后,采用含有磁珠的TruCOUNT试管,用小鼠抗人FITC-CD61抗体和大鼠抗小鼠PE-CD41抗体染色细胞,流式细胞仪定量分析小鼠全血中复苏的hPLTs:以输注后5 min采集的小鼠全血中的hPLTs数量作为基数值,其余时间点的hPLTs数值分别与之比较,计算hPLTs输注后的复苏率。统计学分析比较hPLTs输注后,在WT和SCID小鼠体内的复苏率。结果WT和SCID小鼠体内hPLTs的复苏率,在输注后120 min分别为(37.15±10.39)%和(54.88±18.33)%(t=0.002,P<0.01);在输注后240 min分别为(20.69±8.44)%和(51.07±11.17)%(t=0.000,P<0.01);在输注后360 min在SCID小鼠体内的复苏率为(36.20±7.50)%。结论输注hPLTs后120和240 min,SCID小鼠体内hPLTs的复苏率明显高于WT小鼠,提示SCID小鼠可能作为一种动物模型替代人体实验来评价在体hPLTs的功能。

关键词：人血小板(hPLTs) 实验动物模型野生(WT)小鼠严重联合免疫缺陷(SCID)小鼠输血 hPLTs复苏

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流式细胞术检测小鼠全血中人血小板方法的建立

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广东医学 2010年第16期31卷 2049-2052页

作者：聂咏梅 deborah F.Dumont Larry j.Dumont 广州血液中心广州510095 Dartmouth-Hitchcock Medical Center Lebanon

目的建立一种流式细胞术检测动物模型小鼠全血中人血小板(hPLTs)的方法。方法用CO2致死SCID小鼠,立即心脏穿刺取血,用EDTA抗凝小鼠全血,备用。无菌分离5mL人的单采血小板备用。用1%多聚甲醛固定hPLTs、小鼠全血及hPLTs/小鼠全血混合血液... 详细信息

目的建立一种流式细胞术检测动物模型小鼠全血中人血小板(hPLTs)的方法。方法用CO2致死SCID小鼠,立即心脏穿刺取血,用EDTA抗凝小鼠全血,备用。无菌分离5mL人的单采血小板备用。用1%多聚甲醛固定hPLTs、小鼠全血及hPLTs/小鼠全血混合血液(V/V=1∶4),4℃过夜保存。用洗涤溶液(1%PBS+1%FBS)洗涤和重新悬浮血液,将实验分为阳性对照组、阴性对照组和实验组,用相应的样本与相应抗体反应,建立检测模板。将小鼠全血和hPLTs以1∶4比例混合,用小鼠全血以1∶10、1∶100、1∶1000、1∶10000和1∶100000按体积比稀释上述混合血液,固定,4℃过夜保存。用洗涤溶液洗涤并重新悬浮血液样本,在TruCOUNT试管中定量检测hPLTs,称为"血小板检测值"。用血细胞计数仪检测单采血小板中血小板浓度,称为"血小板期望值"。与血细胞计数仪检测到的"血小板期望值"成对比较,观察可靠的检测浓度范围。结果当血小板期望值介于3000～150000/μL时,与血小板检测值具有较好的线性关系。结论采用流式细胞仪方法,可以可靠地检测到hPLTs期望值介于3000～150000/μL浓度范围内混合在小鼠全血中的hPLTs。

关键词：流式细胞术人血小板小鼠全血抗体

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