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Analysis of a Tropic.l Warm Spring Mic.obiota Using 16S rRNA Metabarc.ding

Advanc.s in Mic.obiology 2020年第4期10卷 145-165页

作者： deborah Fasesan Karim Dawkins Roberto Ramirez Hadiza Rasheed-Jada Abiodun Onilude Oyekanmi Nash Nwadiuto Esiobu c.nter for Genomic. Researc. and Innovation National Biotechnology Development Agency Abuja Nigeria Department of Mic.obiology University of Ibadan Ibadan Nigeria Mic.obial Biotec.nology Laboratory Florida Atlantic University Boca Raton USA

The Ikogosi Warm Spring is a unique ec.logic.l nic.e in Western Nigeria with an average temperature and pH of 38°c.and 5.8 respec.ively. It mixes with an adjac.nt c.ld spring (28°c.& pH 7.6), about 100 meters from sourc., yielding a c.nfluenc. body of water of 32°c.and pH 7.7. To explore the bac.erial c.mmunity struc.ure of this unc.mmon environment and to sc.n for potentially useful bac.eria, metagenomes extrac.ed direc.ly from five samples (sourc. and mid-point of warm spring;sourc. and midpoint of c.ld spring, and the c.nfluenc.) were analyzed. Using the MiSeq Illumina next generation sequenc.ng protoc.ls, the V3-V4 region of the 16S rRNA gene pool was sequenc.d and analyzed by QIIME (Quantitative Insights into Mic.obial Ec.logy) and R software. At least 11% (47,446) of all the sequenc.s were unknown to any of the databases employed. Bac.erial diversity and abundanc. at the sourc. of both springs were extremely low, ac.ounting for less than 0.07% of the total sequenc. reads at the c.nfluenc., 100 m downstream. In c.ntrast to the highly diversified mesophilic.c.nfluenc. c.mmunity where 21 different phyla were identified, only 4 and 5 phyla were rec.vered from the sourc.-point of the warm spring and c.ld spring respec.ively. The most prevalent phyla in all samples were members of the versatile Proteobac.eria (35% - 50% relative abundanc.), and the hardy Firmic.tes (33% - 40%). Operational taxonomic.units (OTUs) obtained from all the samples averaged at 1414. Temperature and pH were equally signific.nt predic.ors of genomic.diversity and ric.ness, with the warm and c.ld spring sourc.s having less than 5 bac.eria phyla. Exiguobac.erium sp. (a potential plastic.degrader) and other deep rooted bac.eria were found in the warm spring while the c.ld spring outflow c.ntained among others suc. as Rubrobac.er sp. and c.loroflexi sp. (whic. is c.ose to the phylogenetic.root of the domain Bac.eria). Many taxonomic.lly unresolved sequenc.s c.uld indic.te the presenc. of potenti

关键词： Ikogosi Warm Springs Amplic.n Metagenomic. Next-Gen Sequenc.ng QIIME Bac.erial Diversity Sourc.

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A Th2-sc.re in the tumor mic.oenvironment as a predic.ive biomarker of response to Bac.llus c.lmette Guérin in patients with non-musc.e invasive bladder c.rc.noma:A retrospec.ive study

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Onc.logy Researc. 2023年第2期31卷 207-220页

作者： GUSTAVO MARTÍN VILLOLDO MARÍA TERESA POMBO MARIANA ARIS JOAQUÍN c.EMI PABLO MANDÓ SUPRIYA NAGARAJU JUAN c.MEAN ADRIÁN BURIONI deborah EGEA MORA AMAT JOSÉLEÓN MELLADO JOSÉMORDOH ALBERTO VILLARONGA MARÍA MARc.LA BARRIO Urology Department Instituto Alexander FlemingCiudad Autónoma de Buenos Aires1426Argentina Immunohistoc.emistry Department Instituto Alexander FlemingCiudad Autónoma de Buenos Aires1426Argentina c.ntro de Investigac.ones Onc.lógic.s Fundación Cáncer FUCACiudad Autónoma de Buenos Aires1426Argentina Urology Department MD Anderson Cancer CenterHoustonTX77030USA Pathology Department Instituto Alexander FlemingCiudad Autónoma de Buenos Aires1426Argentina

Intravesic.l Bac.llus c.lmette Guerin(Bc.)is the gold standard therapy for intermediate/high-risk nonmusc.e invasive bladder c.nc.r(NMIBc..However,the response rate is~60%,and 50%of non-responders will progress to musc.e-invasive *** induc.s massive loc.l infiltration of inflammatory c.lls(Th1)and ultimately c.totoxic.tumor *** searc.ed for predic.ive biomarker of Bc. response by analyzing tumor-infiltrating lymphoc.te(TIL)polarization in the tumor mic.oenvironment(TME)in pre-treatment ***-treatment biopsies from patients with NMIBc.who rec.ived adequate intravesic.l instillation of Bc.(n=32)were evaluated retrospec.ively by *** polarization was assessed by quantifying the T-Bet+(Th1)and GATA-3+(Th2)lymphoc.te ratio(G/T),and the density and degranulation of EPX+*** addition,PD-1/PD-L1 staining was *** results c.rrelated with Bc. *** most non-responders,Th1/Th2 markers were c.mpared in pre-and post-Bc. *** was 65.6%in the study *** responders had a higher G/T ratio and a greater number of degranulated EPX+*** c.mbined into a Th2-sc.re showed a signific.nt assoc.ation with higher sc.res in responders(p=0.027).A Th2-sc.re c.t-off value>48.1 allowed disc.imination of responders with 91%sensitivity but lower ***-free survival was signific.ntly assoc.ated with the Th2-sc.re(p=0.007).In post-Bc. biopsies from rec.rring patients,TILs inc.eased Th2-polarization,probably reflec.ing Bc. failure to induc. a pro-inflammatory status and,thus,a lac. of ***-L1/PD-1 expression was not assoc.ated with the response to *** results support the hypothesis that a preexisting Th2-polarized TME predic.s a better response to Bc.,assuming a reversion to Th1 polarization and antitumor ac.ivity.

关键词： Non-musc.e invasive bladder c.nc.r Bc. predic.ive biomarkers Lymphoc.te polarization

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Amino ac.d quality indic.s of the leaves of c.erodendrum volubile

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Journal of c.astal Life Medic.ne 2016年第4期4卷 320-323页

作者： Oc.uko Luc.y Erukainure Folashade Oluwayemisi Owolabi Temiloluwa Adebola Adesioye deborah Olabisi Akinyele Grac. Ijeoma Okonrokwo Department of Food Tec.nology Federal Institute of Industrial ResearchLagosNigeria Tec.nology Transfer Division Federal Institute of Industrial ResearchOshodiNigeria

Objec.ive:To evaluate the amino ac.d profile and quality indic.s of c.erodendrum volubile(***)***:Dried leaves of *** were blended,defatted and subjec.ed to amino ac.d analysis using the tec.nic.n sequential multi-sample amino ac.d *** amino ac.d quality indic.s whic. c.vers for c.emic.l sc.re,essential amino ac.d index,nutritional index,true digestibility,protein digestibility c.rrec.ed amino ac.d sc.re,and digestible indispensable amino ac.d sc.re were evaluated using standard ***:Amino ac.d analysis revealed glutamic.ac.d to have the highest c.nc.ntration,with c.steine having the *** ac.d had the highest c.emic.l sc.re,this was followed by glyc.ne,histidine and arginine,*** least sc.res were observed in serine and *** ac.d had the highest value for true digestibility and protein digestibility c.rrec.ed amino ac.d sc.re,with the least observed in *** indispensable amino ac.d sc.re evaluation showed histidine to have the highest value for infants(birth to 6 months),threonine for c.ildren(6 months to 3 years),while isoleuc.ne was observed to have the highest value for older c.ildren,adolesc.nts and *** essential amino ac.d index value was less than 4,while nutritional index value was less than ***:These results indic.ted the leaves of *** as a potential sourc. of amino ac.ds in the human diet as portrayed by its amino ac.ds profile and qualities.

关键词： Amino ac.ds c.erodendrum volubile Leafy vegetable Malnutrition

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美国农业部林务局实验林场和范围长期研究

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国外科技新书评介 2015年第9期 16-16页

作者： deborah c. Hayes 鲁敏不详中国科学院动物研究所

本书有着很高的科学价值，书中提供了美国用于长期研究复杂多样的生态学过而受到保护的科研场地。该书通过实例，重点讨论公众关注的问题与政策、实验林场和范围、自然资源管理的政策与实践等彼此之间的动态相互作用。很多时候，在一个... 详细信息

本书有着很高的科学价值，书中提供了美国用于长期研究复杂多样的生态学过而受到保护的科研场地。该书通过实例，重点讨论公众关注的问题与政策、实验林场和范围、自然资源管理的政策与实践等彼此之间的动态相互作用。很多时候，在一个研究项目的早期观察到的趋势或者预期可能与几十年的长期研究记录之间会存在矛盾。在美国一些团队的科学家在一些实验场，包括林务局的实验林场，致力于长期的研究。社会的需求和价值观的变化也可能重新定位研究项目。

关键词：美国农业部实验林场林务局围长自然资源管理科学价值相互作用生态学

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Tree suc.ession ac.oss a seasonally dry tropic.l forest and forest-savanna ec.tone in northern Minas Gerais,Brazil

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Journal of Plant Ec.logy 2017年第5期10卷 859-868页

作者： Polyanne A.c.elho Paola Ferreira Santos Eduardo de Paiva Paula deborah M.G.Apgaua Bruno Gini Madeira Gisele c.istina de Oliveira Menino Yule Roberta Ferreira Nunes Rubens M.Santos David Y.P.Tng Department of Forest Sc.enc.s Federal University of LavrasC.P.3037CEP 37200-000LavrasMinas GeraisBrazil Department of General Biology State University of Montes ClarosC.P.126CEP 39401-089Montes ClarosMinas GeraisBrazil Instituto Federal Goiano C.P.66CEP 75901-970Rio VerdeGoiâniaBrazil c.ntre for Tropic.l Environmental and Sustainability Sc.enc.s College of Marine and Environmental SciencesJames Cook University14-88 McGregor RdSmithfield QLD 4878CairnsQueenslandAustralia

Aims Understanding suc.ession in tropic.l forest is an important aspec. of vegetation sc.enc.,but to date,suc.essional proc.sses in sea-sonally dry tropic.l forests(SDtFs)have rec.ived muc. less atten-tion than evergreen humid tropic.l *** aim to fill this knowledge *** We investigated vegetation suc.ession in SDtF areas c.nsisting of three different suc.essional stages(early,intermediate,late),and a SDtF-savanna ec.tone in the munic.pal distric. of Juramento,north of Minas Gerais State,*** twelve 400 m2 plots in eac. area,we c.mpared vegetation parameters and struc.ural variables(absolute density and basal area)and examined the floristic.c.mposition of the tree c.mponent to find gradients of *** Findings We found evidenc. of spec.es turnover along a suc.essional gradi-ent,with the intermediate stage showing the highest spec.es ric.-ness and *** was ac.ompanied with a signific.nt inc.ease in the number of tree individuals and basal area from the early to intermediate suc.essional ***,the intermediate and late SDtF suc.essional stages were more similar in struc.ure and *** ec.tone was the most spec.es ric. and was similar to the intermediate SDtF and early suc.essional stage in spec.es ric.ness and floristic.c.mposition *** results will have implic.tions for guiding SDtF management and rec.very programs.

关键词： c.atinga floristic.Similarity ec.tone forest suc.ession resilienc..

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SARS-c.V-2 spike variants differ in their allosteric.responses to linoleic.ac.d

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Journal of Molec.lar c.ll Biology 2023年第3期15卷 55-66页

作者： A.Sofia F.Oliveira deborah K.Shoemark Andrew D.Davidson Imre Berger c.ristiane Sc.affitzel Adrian J.Mulholland Sc.ool of c.emistry Centre for Computational ChemistryUniversity of BristolBristol BS81TSUK Sc.ool of c.emistry University of BristolBristol BS81TSUK Sc.ool of Bioc.emistry University of BristolBristol BS81TDUK Sc.ool of c.llular and Molec.lar Medic.ne University of BristolUniversity WalkBristol BS81TDUK Sc.ool of c.emistry Max Planck Bristol Centre for Minimal BiologyBristol BS81TSUK

The SARS-c.V-2 spike protein c.ntains a func.ionally important fatty ac.d(FA)binding site,whic. is also found in some other c.ronaviruses,***-c.V and *** oc.upanc. of the FA site by linoleic.ac.d(LA)reduc.s infec.ivity by‘loc.ing’the spike in a less infec.ious ***,we use dynamic.l-nonequilibrium molec.lar dynamic.(D-NEMD)simulations to c.mpare the allosteric.responses of spike variants to LA removal.D-NEMD simulations show that the FA site is c.upled to other func.ional regions of the protein,*** rec.ptor-binding motif(RBM),N-terminal domain(NTD),furin c.eavage site,and regions surrounding the fusion peptide.D-NEMD simulations also identify the allosteric.networks c.nnec.ing the FA site to these func.ional *** c.mparison between the wild-type spike and four variants(Alpha,Delta,Delta plus,and Omic.on BA.1)shows that the variants differ signific.ntly in their responses to LA *** allosteric.c.nnec.ions to the FA site on Alpha are generally similar to those on the wild-type protein,with the exc.ption of the RBM and the S71–R78 region,whic. show a weaker link to the FA *** c.ntrast,Omic.on is the most different variant,exhibiting signific.nt differenc.s in the RBM,NTD,V622–L629,and furin c.eavage *** differenc.s in the allosteric.modulation may be of func.ional relevanc.,potentially affec.ing transmissibility and *** c.mparison of the effec.s of LA on SARS-c.V-2 variants,inc.uding emerging variants,is warranted.

关键词： SARS-c.V-2 spike allosteric.modulation D-NEMD simulations fatty ac.d binding site SARS-c.V-2 variants

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风险

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作者： (澳)狄波拉·勒普顿(deborah Lupton)

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Water Quality and Ec.toxic.ty Assessment in Surfac. Waters from c.batao River and Surroundings,Sao Paulo,Brazil

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Journal of Water Resourc. and Protec.ion 2017年第12期9卷 1510-1525页

作者： Vanessa Silva Granadeiro Garc.a Eduardo Toshio Domingues Matsushita Letíc.a c.istina Alves Mesquita deborah Ines Teixeira Favaro Sueli Ivone Borrely Instituto de Pesquisas Energétic.s e Nuc.eares IPEN-CNEN/SPCentro de Tecnologia das RadiacoesCidade UniversitáriaSao PauloBrazil Instituto de Fisic. da Universidade de Sao Paulo IF/USPButantaSao PauloBrazil Instituto de Pesquisas Energétic.s e Nuc.eares IPEN-CNEN/SPLaboratório de Análises por Ativacao NeutronicaCidade UniversitáriaSao PauloBrazil

The monitoring of water bodies means the attempt of protec.ing vulnerable groups of organisms inhabiting streams and rivers. Industrial and domestic.disc.arges may worsen the water quality and affec. biologic.l balanc., struc.ure and the func.ioning of the ec.system. c.bat?o c.ty, is one of the largest industrial c.nters in Brazil and in Latin Americ., where the c.nstant disc.arge of effluents into c.bat?o River and its tributaries c.used a degradation sc.nario in the hydrographic.basin of the region. The objec.ive of this study was to evaluate the ac.te and c.ronic.effec.s of surfac. water from c.bat?o River and two of its tributaries (Perequê and Pil?es) by ec.toxic.logic.l assays. In addition, physic.l c.emic.l parameters were analyzed. c.riodaphnia dubia and Vibrio fisc.eri were exposed-organisms during the studied period. The study was c.nduc.ed between 2010 and 2011 in four c.mpaigns and nine sites along the basin of c.bat?o River. The ec.toxic.ty was measured by Vibrio fisc.eri bioluminesc.nc., Ec.0 values ranging from 31.25% to 71.61%. In c.ntrast, based on a bioequivalenc. t-test statistic.l analysis, the results obtained with c.riodaphnia dubia revealed no toxic.ty in the sampling sites. A c.itic.l analysis of raw data of luminesc.nc. was c.rried out showing higher values during the 2nd c.mpaign. From the numbers obtained for physic.l-c.emic.l parameters P5 was far the worst due to c.lorides, hardness and c.nduc.ivity. From the Pearson c.rrelation analysis c.rried out with toxic.ty to V. fisc.eri and the physic.l c.emic.l parameters, the dissolved oxygen in water resulted in a moderate positive c.rrelation. Sediment c.ntamination was also demonstrated in the region.

关键词： c.dubia c.batao River Ec.toxic.ty Assays V.fisc.eri Water Quality

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Impac.s of Forest Harvest on Ac.ive c.rbon and Mic.obial Properties of a Volc.nic.Ash c.p Soil in Northern Idaho

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Open Journal of Soil Sc.enc. 2015年第1期5卷 11-19页

作者： deborah S. Page-Dumroese Matt D. Busse Steven T. Overby Brian D. Gardner Joanne M. Tiroc.e USDA Forest Servic. Rocky Mountain Research Station Moscow USA USDA Forest Servic. Pacific Southwest Research Station Davis USA USDA Forest Servic. Rocky Mountain Research Station Flagstaff USA USDA Natural Resourc. c.nservation Servic. Moscow USA

Soil quality assessments are essential for determining impac.s on belowground mic.obial c.mmunity struc.ure and func.ion. We evaluated the suitability of ac.ive c.rbon (c., a rapid field test, as an indic.tor of soil biologic.l quality in five paired forest stands (c.ear c.t harvested 40 years prior and unharvested) growing on volc.nic.ash-c.p soils in northern Idaho. Ac.ive c.was c.mpared with several traditional measures of soil mic.obial properties (mic.obial biomass, respiration, fungal hyphal biomass, bac.erial number and biomass and PLFA c.mmunity struc.ure). Despite the signific.nt differenc.s in forest vegetation between paired stands, no differenc.s in ac.ive c.and only a few signific.nt differenc.s in mic.obial properties were detec.ed. Total bac.eria (mic.osc.pe c.unts) and PLFA signatures (gram positive bac.eria, gram negative bac.eria, ac.inomyc.tes) were signific.ntly higher in the managed stands. Our results indic.te that either mineral soil biologic.l properties in managed stands were relatively unaffec.ed at the time of harvest or some biologic.l rec.very oc.urred 40 years later. Additionally, volc.nic.ash-c.p soils in moist ec.systems c.uld be highly resilient to the impac.s of harvest operations and therefore few signific.nt biologic.l c.anges c.uld be detec.ed.

关键词： Soil Quality Forest Soil Soil Monitoring Soil Organic Matter

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城市与城市文化

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作者： (澳)德波拉·史蒂文森(deborah Stevenson)

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