检索结果-南通市图书馆

ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells

World Journal of Stem Cells 2021年第11期13卷 1783-1796页

作者： Giuliana Mannino Martina Cristaldi Giovanni Giurdanella Rosario Emanuele Perrotta debora lo furno Rosario Giuffrida Dario Rusciano Department of Biomedical and Biotechnological Sciences University of CataniaCatania 95123CTItaly Research Center SOOFT-Italia S.p.A.Catania 95123CTItaly Department of General Surgery and Medical-Surgery Specialties University of CataniaCatania 95100CTItaly

BACKGROUND Adipose-derived stem cells(ASCs)have been increasingly explored for cell-based medicine because of their numerous advantages in terms of easy availability,high proliferation rate,multipotent differentiation ability and low *** this respect,they have been widely investigated in the last two decades to develop therapeutic strategies for a variety of human pathologies including eye *** ocular diseases involving the retina,various cell types may be affected,such as Müller cells,astrocytes,photoreceptors and retinal pigment epithelium(RPE),which plays a fundamental role in the homeostasis of retinal tissue,by secreting a variety of growth factors that support retinal *** To test ASC neural differentiation using conditioned medium(CM)from an RPE cell line(ARPE-19).METHODS ASCs were isolated from adipose tissue,harvested from the subcutaneous region of healthy donors undergoing liposuction *** ASC culture conditions were investigated:ASCs cultured in basal Dulbecco's Modified Eagle Medium(DMEM);ASCs cultured in serum-free DMEM;ASCs cultured in serumfree DMEM/F12;and ASCs cultured in a CM from ARPE-19,a spontaneously arising cell line with a normal karyotype derived from a human *** proliferation rate and viability were assessed by crystal violet and MTT assays at 1,4and 8 d of *** the same time points,ASC neural differentiation was evaluated by immunocytochemistry and western blot analysis for typical neuronal and glial markers:Nestin,neuronal specific enolase(NSE),protein gene product(PGP)9.5,and glial fibrillary acidic protein(GFAP).RESULTS Depending on the culture medium,ASC proliferation rate and viability showed some significant ***,less dense populations were observed in serum-free cultures,except for ASCs cultured in ARPE-19 serum-free ***,a different cell morphology was seen in these cultures after 8 d of treatment,with more elongated cells,often showing cytoplasmic ramifications.

关键词： Adipose-derived mesenchymal stem cells Retinal pigment epithelium Neural markers Neural differentiation Retina damage Cell-based medicine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Potential therapeutic applications of mesenchymal stem cells for the treatment of eye diseases

引用

World Journal of Stem Cells 2021年第6期13卷 632-644页

作者： Giuliana Mannino Cristina Russo Anna longo Carmelina Daniela Anfuso Gabriella Lupo debora lo furno Rosario Giuffrida Giovanni Giurdanella Department of Biomedical and Biotechnological Sciences School of MedicineUniversity of CataniaCatania 95123Italy

Stem cell-based treatments have been extensively explored in the last few decades to develop therapeutic strategies aimed at providing effective alternatives for those human pathologies in which surgical or pharmacological therapies produce limited *** stem cells of different sources,mesenchymal stem cells(MSCs)offer several advantages,such as the absence of ethical concerns,easy harvesting,low immunogenicity and reduced tumorigenesis *** than a multipotent differentiation ability,MSCs can release extracellular vesicles conveying proteins,mRNA and *** to these properties,new therapeutic approaches have been designed for the treatment of various pathologies,including ocular *** this review,the use of different MSCs and different administration strategies are described for the treatment of diabetic retinopathy,glaucoma,and retinitis *** a large number of investigations,positive results have been obtained by in vitro experiments and by MSC administration in animal *** authors agree that beneficial effects are likely related to MSC paracrine *** on these considerations,many clinical trials have already been carried ***,although some adverse effects have been described,promising outcomes are *** can be assumed that in the near future,safer and more effective protocols will be developed for more numerous clinical applications to improve the quality of life of patients affected by eye diseases.

关键词： Mesenchymal stem cells Eye diseases Diabetic retinopathy Glaucoma Retinitis pigmentosa Regenerative medicine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pericyte-like differentiation of human adipose-derived mesenchymal stem cells:An in vitro study

引用

World Journal of Stem Cells 2020年第10期12卷 1152-1170页

作者： Giuliana Mannino Florinda Gennuso Giovanni Giurdanella Federica Conti Filippo Drago Salvatore Salomone debora lo furno Claudio Bucolo Rosario Giuffrida Physiology Section Department of Biomedical and Biotechnological SciencesUniversity of CataniaCatania 95123Italy Pharmacology Section Department of Biomedical and Biotechnological SciencesSchool of MedicineUniversity of CataniaCatania 95123Italy Biochemistry Section Department of Biomedical and Biotechnological SciencesSchool of MedicineUniversity of CataniaCatania 95123Italy

BACKGROUND Adipose-derived mesenchymal stem cells(ASCs)are characterized by long-term self-renewal and a high proliferation *** adequate conditions,they may differentiate into cells belonging to mesodermal,endodermal or ectodermal *** support endothelial cells and play an important role in stabilizing the vessel wall at the microcirculation *** loss of pericytes,as occurs in diabetic retinopathy,results in a breakdown of the blood-retina barrier(BRB)and infiltration of inflammatory *** this context,the use of pericytelike differentiated ASCs may represent a valuable therapeutic strategy for restoring BRB *** To test in vitro strategies to obtain pericyte-like differentiation of human ASCs(hASCs).METHODS Different culture conditions were tested:hASCs cultured in a basal medium supplemented with transforming growth factorβ1;and hASCs cultured in a specific pericyte medium(PM-hASCs).In a further sample,pericyte growth supplement was omitted from the *** addition,cultures of human retinal pericytes(hRPCs)were used for ***-like differentiation of hASCs was tested by immunocytochemical staining and western blotting to evaluate the expression ofα-smooth muscle actin(α-SMA)and neural/glial antigen 2(NG2).Interactions between human retinal endothelial cells(hRECs)and different groups of hASCs were investigated in co-culture *** these cases,the expression of typical junctional proteins such as vascular endothelial-Cadherin,zonula occludens-1 and Occludin were assessed in *** an in vitro model of the BRB,values of trans-endothelial electrical resistance were measured when hRECs were co-cultured with various groups of pretreated *** values observed were compared with co-cultures of hRECs and hRPCs as well as with cultures of hRECs ***-dimensional co-cultures of hRECs and hRPCs or pericyte-like hASCs in Matrigel were designed to assess their reciprocal *** After 3-6 d of cult

关键词： Adipose-derived mesenchymal stem cells Pericyte-like differentiation Retinal endothelial cells Retinal pericytes Blood-retina barrier Junction proteins

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

How Secure Having IoT Devices in Our Homes?

引用

Journal of Information Security 2020年第2期11卷 81-91页

作者： debora Estrada lo’ai Tawalbeh Robert Vinaja Department of Computing and Cyber Security Texas A&M University San Antonio Texas USA

Nowadays, technology has evolved to be in our daily lives to assist in making our lives easier. We now have technology helping us in our lives at home. Devices used to create our “smart home” have done a great deal in making our lives at home less burdensome, but sadly, these devices have secured our personal lives to be more accessible to outsiders. In this paper, the security of home smart devices and their communication will be researched by using other academic articles to support facts found. The operation of the devices will be discussed along with security risks and future trends on security attacks. The results found will be crucial to knowing exactly how well our own home is protected. After understanding where the risks lie and a demonstration of how hackers can take control of our smart home, solutions will be given to shield ourselves from security attacks. We protect our homes from physical threats by locking doors, but it is time we guard ourselves from cyber threats as well.

关键词： IoT Smart Home Cloud

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：