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HAF1 mediates circadian accumulation of Os ELF3 in determining the heading date of rice under long-day conditions

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HAF1 mediates circadian accumulation of Os ELF3 in determini...

Bract suppression regulated by the miR156/529-SPLs-NL1-PLA1 module is required for the transition from vegetative to reproductive branching in rice

2016年全国植物生物学大会

作者： Chunmei Zhu Qiang Peng debao fu Dongxia Zhuang Changyin Wu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Plant circadian clock has been provided to be involved in multiple physiology and metabolism process by monitoring external environment changes,including photoperiodic flowering regulation. Our previous study showed that Os ELF3 acts as a floral activator in the long-day photoperiodic pathway via its crosstalk with the circadian clock in rice. However,post-transcription regulation of Os ELF3 is still unclear. In this study,we showed that HAF1,an E3 ubiquitin ligase,physically interact with Os ELF3 in vivo and in vitro. C-terminal domain of Os ELF3 was identified as the functional motif for mediating interaction with HAF1,as well as formation of homodimer by itself. further protein degradation assay indicated that Os ELF3 is the direct substrate of HAF1 for ubiquitination in rice. Genetically,the oself3 haf1 double mutant headed as late as oself3 under long-day conditions. Previous investigation revealed that Os ELF3 plays a crucial role in photoperiodic response to determine rice regional adaptability. We proposed that HAF1 plays a pivotal role in heading date modulation by ubiquitination of Os ELF3 under long-day conditions.

关键词： circadian clock photoperiodic flowering ubiquitination rice

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Molecular Plant 2021年第7期14卷 1168-1184页

作者： Lei Wang Luchang Ming Keyan Liao Chunjiao Xia Shengyuan Sun Yu Chang Hongkai Wang debao fu Conghao Xu Zhengji Wang Xu Li Weibo Xie Yidan Ouyang Qinglu Zhang Xianghua Li Qinghua Zhang Jinghua Xiao Qifa Zhang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China

Reproductive transition of grasses is characterized by switching the pattern of lateral branches,featuring the suppression of outgrowth of the subtending leaves(bracts)and rapid formation of higher-order branches in the inflorescence(panicle).However,the molecular mechanisms underlying such changes remain largely ***,we show that bract suppression is required for the reproductive branching in *** identified a pathway involving the intrinsic time ruler microRNAI56/529,their targets SQUAMOSA PROMOTER BINDING PROTEIN LIKE(SPL)genes,NECK LEAF1(NL1),and PLASTOCHRON1(PLA1),which regulates the bract outgrowth and thus affects the pattern switch between vegetative and reproductive *** of the bract results in global reprogramming of transcriptome and chromatin accessibility following the reproductive transition,while these processes are largely dysregu-lated in the mutants of these *** discoveries contribute to our understanding of the dynamic plant architecture and provide novel insights for improving crop yields.

关键词： grass plant architecture developmental timing lateral branch bract suppression

The PMM1 gene is involved in brassinosteroid biosynthesis and determines inflorescence architecture in rice

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The PMM1 gene is involved in brassinosteroid biosynthesis an...

第十三届全国植物基因组学大会

作者： Xuemei Li,debao fu,Yan Li,Guoxing Chen,Changyin Wu~* National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(wuhan),Huazhong Agricultural University,Wuhan 430070,China

Panicle morphology is one of the main determinants for the yield in *** order to understand the mechanism of inflorescence architecture in rice,the Panicle Morphology Mutant 1(PMM1) was isolated by a map-based cloning...

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对“低碳”问题的再讨论

林业勘查设计 2011年第2期40卷 -页

作者：张兴华付德宝黑龙江省穆棱林业局

实木不环保,不‘低碳’。顺应低碳时代需求，从身边做起，从自身做起，将会有一片树林、一片蓝天。

关键词：实木陆地生态系统主体

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制浆黑液中碱木质素的利用开发途径

林业勘查设计 2011年第3期40卷 114-115页

作者：张兴华付德宝黑龙江省穆棱林业局

因为我国森林资源日趋紧张,造纸工业原料约86%采用禾本科作物秸秆和草类原料,主要采用碱法制浆,此种原料制浆的主要副产品———碱木质素。那么通过对碱木质素的改性,可以有效地开发碱木质素产品,用途广泛。

关键词：碱木质素产品合理利用

Multi-objective optimization of frequency and damping of vertical stabilizer skin structure placed with variable-angle tows

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Chinese Journal of Aeronautics 2023年第1期36卷 444-455页

作者： Lei ZU Xianzhao XIA Qian ZHANG Guiming ZHANG Shijun CHEN Jianhui fu Lichuan ZHOU Huabi WANG debao LI Liqing ZOU School of Mechanical Engineering Hefei University of TechnologyHefei 230009China Anhui Province Key Laboratory of Aerospace Structural Parts Forming Technology and Equipment Hefei University of TechnologyHefei 230009China

The vibration characteristics of composite vertical stabilizer skin structures play a critical role in damping effects designed for overcoming the air disturbances experienced by aircraft structural components during *** first-order fundamental frequencies and their corresponding damping characteristics of the vertical stabilizer skin structure tow-steered by automatic fiber placement technique were optimized with the parameterized trajectories and plies as design ***,the vibration and damping numerical models were derived based on Kirchhoff laminate theory,the Rayleigh-Ritz method,and the Strain Energy *** the optimization model was developed by adopting the self-adaptive Differential Evolution Multi-objective optimization algorithm and incorporating the solution method of Pareto *** constraints of this optimization model considered the experimentally obtained minimum turning radius of prepregs tow-steered in automatic fiber placement process obtained from experimental ***,the comparison of numerical simulation results was conducted for the optimized trajectories and the conventional straight trajectories under various boundary conditions,and the numerical results were partially validated through damping and frequency *** results indicate the vibration characteristics of the composite vertical stabilizer skin structure can be enhanced to a large extent by optimizing fiber trajectories,and the enhancement percentage is affected by the boundary conditions of the actual structure.

关键词： Damping fundamental frequency Multi-objective optimiza-tion Variable-angle tow Vertical stabilizer skin Vibration properties

一个控制水稻侧生小穗形成基因的遗传定位分析

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分子植物育种 2015年第3期13卷 483-490页

作者：冯志赟符德保张亚芳马玉银李玲梅吴昌银华中农业大学作物遗传改良国家重点实验室华中农业大学国家植物基因研究中心武汉430070 扬州大学教育部植物功能基因组学重点实验室扬州225009 扬州市职业大学生化工程学院扬州225009

水稻的穗是一个典型的圆锥花序,其发育形态直接影响水稻产量。本研究利用本课题组创建的水稻"中花11号"T-DNA插入突变体库,通过表型筛选鉴定了一个稀穗突变体。该突变体在营养生长阶段表现为分蘖数减少,生殖生长阶段稻穗一次枝梗发育正... 详细信息

水稻的穗是一个典型的圆锥花序,其发育形态直接影响水稻产量。本研究利用本课题组创建的水稻"中花11号"T-DNA插入突变体库,通过表型筛选鉴定了一个稀穗突变体。该突变体在营养生长阶段表现为分蘖数减少,生殖生长阶段稻穗一次枝梗发育正常、但二次枝梗数和侧生小穗数目显著减少,表现为明显的稀穗表型。分子检测结果表明该突变体不是由T-DNA插入导致的。通过与籼稻品种"珍汕97"构建F2遗传定位群体遗传分析表明:该突变性状受一个隐性基因所控制。采用图位克隆的方法将目标基因定位于4号染色体上的SSR标记RM16880和RM1205之间约120 kb范围内。基因组序列分析表明:该突变体是由于LAX2基因序列缺失60 bp所导致。该突变体是LAX2的一个新的等位突变体,命名为lax2-4。lax2-4为研究LAX2控制水稻穗形态建成的分子机制积累了遗传材料。

关键词：水稻侧生小穗基因定位 LAX2

PPARA基因对绵羊肌内脂肪沉积的影响及其多态性

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华北农学报 2024年第2期39卷 209-218页

作者：付玲娟史金平张全伟刘婷赵德宝马维华马海蛟孟泉禄唐致雄成述儒甘肃农业大学动物科学技术学院甘肃兰州730070 甘肃农业大学生命科学技术学院甘肃兰州730070 甘肃省临夏州康乐县畜牧兽医中心甘肃临夏731500

为研究PPARA对绵羊肌内脂肪(IMF)沉积的影响及作用机制,寻找绵羊肌内脂肪沉积相关分子标记,以小尾寒羊(STH)和萨寒杂交(STH×SFK)F1群体为研究对象,通过测定其肉品质,利用H&E染色和油红O染色比较2个绵羊群体背最长肌组织学结构及脂滴分... 详细信息

为研究PPARA对绵羊肌内脂肪(IMF)沉积的影响及作用机制,寻找绵羊肌内脂肪沉积相关分子标记,以小尾寒羊(STH)和萨寒杂交(STH×SFK)F1群体为研究对象,通过测定其肉品质,利用H&E染色和油红O染色比较2个绵羊群体背最长肌组织学结构及脂滴分布;利用qRT-PCR和WB技术检测PPARA mRNA水平和蛋白水平在不同群体间的表达差异,并分析PPARA基因与肌内脂肪的相关性;Sanger测序技术检测PPARA基因SNP位点以评估其作为绵羊肌内脂肪沉积相关遗传标记的可能性。结果表明:小尾寒羊剪切力和失水率极显著高于萨寒杂交,但是pH值极显著低于萨寒杂交,大理石花纹评分小尾寒羊低于萨寒杂交群体;组织学染色显示,小尾寒羊肌纤维较粗,排列紧密,肌内脂肪在肌纤维间隙集中分布;萨寒杂交肌纤维较细且结构松散,肌内脂肪在肌细胞间及肌纤维间隙均匀、广泛分布,含量更高;PPARA mRNA表达量小尾寒羊群体显著高于萨寒杂交群体,PPARA蛋白表达量小尾寒羊群体极显著高于萨寒杂交群体;相关性分析显示,PPARA基因表达量与绵羊脂滴面积比及pH值呈极显著负相关,与剪切力和失水率呈极显著正相关,与大理石花纹评分弱相关;Sanger测序结果分析显示,2个绵羊群体PPARA基因第2内含子T49885C、T50007C、G50013A、G50835A、G50942A及G51154C位置上发生碱基突变,但小尾寒羊群体未检测到C49885T突变。SNP遗传多样性分析显示,SNP位点群体纯合度均大于杂合度,在群体中处于中低度多态;除G50835A突变偏离了Hardy-Weinberg平衡,其余SNPs均符合Hardy-Weinberg平衡,遗传基础稳定。研究认为,PPARA基因对绵羊肌内脂肪沉积具有重要影响,可作为绵羊肌内脂肪性状选择的潜在分子遗传标记。

关键词：绵羊肌内脂肪 PPARA 多态性

miR-16a对牛肌卫星细胞增殖和分化的影响

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黑龙江畜牧兽医 2024年第3期 54-59页

作者：夏晓霞赵一苹符梦云张林林李新郭宏胡德宝丁向彬郭益文天津农学院动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室天津300384

为了探究miR-16a对牛肌卫星细胞增殖和分化的影响,试验通过NCBI和Ensembl数据库分析整合miR-16a的位置及信息,用miR-16a mimics转染牛肌卫星细胞,将牛肌卫星细胞分为试验组(miR-16a mimics)和对照组(NC),采用实时荧光定量PCR扩增、Weste... 详细信息

为了探究miR-16a对牛肌卫星细胞增殖和分化的影响,试验通过NCBI和Ensembl数据库分析整合miR-16a的位置及信息,用miR-16a mimics转染牛肌卫星细胞,将牛肌卫星细胞分为试验组(miR-16a mimics)和对照组(NC),采用实时荧光定量PCR扩增、Western-blot检测和EdU细胞增殖试验对转染处理后的牛肌卫星细胞的增殖和分化两个时期进行研究。结果表明:miR-16a位于牛12号染色体的反义链上,并由前体bta-miR-16a的5′端加工生成成熟的miR-16a。在增殖期,与对照组比较,试验组增殖标志因子Pax7 mRNA和其蛋白相对表达量显著或极显著下调(P<0.05或P<0.01),EdU标记指数显著降低(P<0.05)。在分化期,与对照组比较,试验组分化标志因子MyoG mRNA相对表达量极显著下调(P<0.01)。说明miR-16a对牛肌卫星细胞的增殖和分化均存在抑制作用。

关键词：牛牛肌卫星细胞 miR-16a 增殖分化