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OsRLR4 binds to the OsAUX1 promoter to negatively regulate primary root development in rice

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Journal of Integrative Plant Biology 2022年第1期64卷 118-134页

作者： Chendong Sun Dongming Li Zhenyu Gao Lei Gao Lianguang Shang Mei Wang Jiyue Qiao Shilin Ding Chuanyou Li Markus Geisler dean jiang Yanhua Qi Qian Qian Key Laboratory of Herbage&Endemic Crop Biology of Ministry of Education Inner Mongolia Key Laboratory of Herbage&Endemic Crop BiotechnologySchool of Life SciencesInner Mongolia UniversityHohhot 010000China State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province College of Advanced Agricultural SciencesZhejiang Agriculture and Forestry UniversityLin'an Hangzhou 311300China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 310006China College of Life Sciences and Oceanography Guangdong Provincial Key Laboratory for Plant EpigeneticsShenzhen UniversityShenzhenChina Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518120China State Key Laboratory of Plant Genomics National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyThe Chinese Academy of SciencesBeijing 100101China Department of Biology University of FribourgFribourg CH-1700Switzerland

Root architecture is one of the most important agronomic traits that determines rice crop yield. The primary root(PR) absorbs mineral nutrients and provides mechanical support;however, the molecular mechanisms of PR elongation remain unclear in rice. Here, the two loss-of-function T-DNA insertion mutants of root length regulator 4(Os RLR4), osrlr4-1 and osrlr4-2 with longer PR, and three Os RLR4 overexpression lines, OE-Os RLR4-1/-2/-3 with shorter PR compared to the wild type/Hwayoung(WT/HY), were identified. Os RLR4 isone of five members of the PRAF subfamily of the regulator chromosome condensation1(RCC1) family. Phylogenetic analysis of Os RLR4 from wild and cultivated rice indicated that it is under selective sweeps, suggesting its potential role in domestication. Os RLR4 controls PR development by regulating auxin accumulation in the PR tip and thus the root apical meristem activity. A series of biochemical and genetic analyses demonstrated that Os RLR4 functions directly upstream of the auxin transporter Os AUX1. Moreover, Os RLR4 interacts with the TRITHORAX-like protein Os Trx1 to promote H3 K4 me3 deposition at the Os AUX1 promoter, thus altering its transcription level. This work provides insight into the cooperation of auxin and epigenetic modifications in regulating root architecture and provides a genetic resource for plant architecture breeding.

关键词： auxin transporter primary root development regulator chromosome condensation 1 rice root length regulator

MsYSL1 of Miscanthus sacchariflorus enhances resistance of Arabidopsis to cadmium by mediating metal ion reallocation

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MsYSL1 of Miscanthus sacchariflorus enhances resistance of A...

第七届长三角植物科学研讨会暨青年学术报告会

作者： Cheng Zhang Houming Chen Haipeng Guo dean jiang College of Life Sciences Zhejiang University

The Yellow Stripe-Like(YSL) family of transporters mediates the uptake,translocation,and distribution of various mineral elements in vivo by transferring metal ions chelated with phytosiderophore or nicotianamine(NA).However,little is known about the roles of the YSL genes against cadmium in *** this study,we first cloned and characterized a vital member of the YSL gene family,Ms YSL1,from the bioenergy plant Miscanthus *** YSL1 localized in the plasma membrane and was widely expressed throughout the whole seedling with the highest expression level in the *** addition,its expression in the root was stimulated by excess manganese(Mn),cadmium(Cd),and lead,and a shortage of iron(Fe),zinc(Zn),and *** complementation in yeast indicated that Ms YSL1 showed transport activity for Fe(II)-NA and Zn-NA,but not for *** they exhibited no significant differences versus the wild type under normal cultivation conditions,Ms YSL1-overexpressing Arabidopsis lines displayed a higher resistance to Cd accompanied by longer root lengths,lower Cd,Zn,and Mn levels in roots,and higher Cd,Fe,and Mn translocation ratios under Cd ***,genes related to NA synthesis,metal translocation,long-distance transport,and Cd exclusion were highly induced in transgenic lines under Cd ***,MsYSL1 may be an essential transporter for diverse metal-NAs to participate in the Cd detoxification by mediating the reallocation of other metal ions.

关键词： Miscanthus sacchariflorus MsYSL1 Yeast complementation Cd tolerance Arabidopsis

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橡胶沥青紫外光老化性能试验研究

南京航空航天大学学报 2013年第1期45卷 152-156页

作者：肖鹏吴美平蒋德安扬州大学建筑科学与工程学院扬州225127

为了研究橡胶沥青紫外光老化后的性能变化,使用布氏粘度仪、动态剪切流变仪、弯曲流变仪测试紫外光老化后的橡胶沥青的软化点、粘度、疲劳因子、蠕变劲度和蠕变速率。试验结果表明:随着紫外光老化时间的延长,橡胶沥青高温稳定性有所提高... 详细信息

为了研究橡胶沥青紫外光老化后的性能变化,使用布氏粘度仪、动态剪切流变仪、弯曲流变仪测试紫外光老化后的橡胶沥青的软化点、粘度、疲劳因子、蠕变劲度和蠕变速率。试验结果表明:随着紫外光老化时间的延长,橡胶沥青高温稳定性有所提高,抗疲劳开裂能力和低温性能逐渐降低。紫外老化温度高于60°C时,橡胶沥青受紫外光老化和热氧老化共同作用,软化点和粘度增幅较大,抗疲劳开裂能力和低温性能也受到显著影响。通过灰关联分析可知,随着紫外光老化时间的延长和老化温度的上升,橡胶沥青老化后的抗疲劳开裂能力和低温性能受到显著影响。同时得出蠕变速率不适合作为评价沥青紫外光老化后性能变化的结论。

关键词：橡胶沥青紫外光老化抗疲劳开裂能力低温性能灰关联分析

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玄武岩高强纤维沥青混合料路用性能试验研究

施工技术 2015年第4期44卷 95-97,101页

作者：肖鹏吕阳蒋德安扬州大学建筑科学与工程学院江苏扬州225127

对掺入玄武岩高强纤维的AC-13C级配的基质沥青和SBS改性沥青混合料进行了路用性能研究,并将其与未掺入玄武岩高强纤维的沥青混合料进行对比。采用车辙、低温弯曲、浸水马歇尔、冻融劈裂以及四点弯曲疲劳等一系列室内试验,分析了其高温... 详细信息

对掺入玄武岩高强纤维的AC-13C级配的基质沥青和SBS改性沥青混合料进行了路用性能研究,并将其与未掺入玄武岩高强纤维的沥青混合料进行对比。采用车辙、低温弯曲、浸水马歇尔、冻融劈裂以及四点弯曲疲劳等一系列室内试验,分析了其高温稳定性、低温抗裂性、水稳定性和抗疲劳性能。试验数据表明,玄武岩高强纤维能明显提升沥青混合料的高温稳定性、低温抗裂性和抗疲劳性能,但对水稳定性的影响需要进一步研究。在AC-13C级配沥青混合料中掺加玄武岩高强纤维能有效改善混合料的路面性能,并且在SBS改性沥青混合料中效果更好。

关键词：玄武岩高强纤维沥青混合料级配路用性能试验

Auxin response factor(OsARF12),a novel regulator for phosphate homeostasis in rice

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Auxin response factor(OsARF12),a novel regulator for phospha...

2013全国植物生物学大会

作者： WANG Suikang ZHANG Saina SUN Chendong XU Yanxia CHEN Yue YU Chenliang QIAN Qian jiang dean QI Yanhua State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang University State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural Sciences

Phosphorus(P)is crucial nutrient element for crop growth and ***,the network pathway regulating homeostasis of phosphate(Pi) in crops has many molecular breeding ***,we repor

关键词： auxin response factor OsARF12 phosphate absorption and transport phosphate homeostasis rice

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模拟酸雨对假俭草叶绿素荧光特性的影响

林业科学 2006年第11期42卷 8-11页

作者：马博英徐礼根蒋德安浙江教育学院生物系杭州310012 浙江大学生命科学学院植物生理与生物化学国家重点实验室杭州310058

研究模拟酸雨(pH2.5、pH3.0)对假俭草叶绿素荧光特性的影响。结果表明:酸雨6h胁迫引起假俭草PSⅡ最大光化学效率(Fv/Fm)、PSⅡ潜在活性(Fv/F0)、光化学猝灭系数(qP)、PSⅡ电子传递量子产率(ФPSⅡ)、光合电子传递速率(ETR)和非光化学猝... 详细信息

研究模拟酸雨(pH2.5、pH3.0)对假俭草叶绿素荧光特性的影响。结果表明:酸雨6h胁迫引起假俭草PSⅡ最大光化学效率(Fv/Fm)、PSⅡ潜在活性(Fv/F0)、光化学猝灭系数(qP)、PSⅡ电子传递量子产率(ФPSⅡ)、光合电子传递速率(ETR)和非光化学猝灭系数(NPQ)下降,pH2.5处理比pH3.0处理的下降幅度大;酸雨处理停止24h后,Fv/Fm、Fv/F0、qP、ФPSⅡ、ETR、NPQ回升;酸雨处理停止48h后,回升至接近对照。研究还表明,利用非损伤的叶绿素荧光技术可快速检测草对环境因子变化的响应。

关键词：模拟酸雨假俭草叶绿素荧光

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影响水稻光合日变化的酶和相关因素的分析

生物数学学报 1999年第4期14卷 495-500页

作者：翁晓燕蒋德安陆庆浙江大学华家池校区生物系杭州310029

水稻叶片的最大光合速率出现在上午１０：００时，Ｒｕｂｉｓｃｏ初始活力也在此时达到最大，然后逐渐降低，下午１４：００时略上升后又下降．Ｒｕｂｉｓｃｏ初始活力与光合速率之间极显著相关，相关系数为０．９４７４．运用相关性... 详细信息

水稻叶片的最大光合速率出现在上午１０：００时，Ｒｕｂｉｓｃｏ初始活力也在此时达到最大，然后逐渐降低，下午１４：００时略上升后又下降．Ｒｕｂｉｓｃｏ初始活力与光合速率之间极显著相关，相关系数为０．９４７４．运用相关性分析、回归分析、通径分析，对晴（有时有云）天气水稻叶片的光合速度、光合关键酶及有关因素的日变化进行综合评估，结果发现中午光合下降主要来自气孔限制，同时Ｒｕｂｉｓｃｏ活性也下降；Ｒｕｂｉｓｃｏ活性是影响光合日变化的又一重要生化因子．体内Ｒｕｂｉｓｃｏ活性受Ｒｕｂｉｓｃｏ活化酶的调节．

关键词：光合速率 Rubisco 通径分析水稻日变化

Two Rubisco Activase Isoforms May Play Different Role in Photosynthetic Heat Acclimation in Rice Plant

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Two Rubisco Activase Isoforms May Play Different Role in Pho...

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Dun Wang, Xiaofei Li, Wanjun Yang, Xuping Feng, dean jiang* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China

＜正＞Studies in many species have shown that increasing growth temperature gradually or pre-treatment can lead

关键词： Rubisco activase isoforms heat acclimation photosynthetic rate

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玄武岩纤维沥青混合料韧性研究及机理分析

南京理工大学学报 2015年第4期39卷 500-505页

作者：吴正光蒋德安吕阳肖鹏丁泽民余晖扬州大学建筑科学与工程学院江苏扬州225127 扬州市公路管理处江苏扬州225002

为研究玄武岩纤维对沥青混合料韧性的影响,选用AC-13C和SMA-13两种混合料,使用UTM-25试验机对添加纤维前后的马歇尔试件进行间接拉伸试验;通过扫描电镜对纤维沥青混合料进行微观观测,分析纤维对混合料韧性的影响及增韧机理。试验结果表... 详细信息

为研究玄武岩纤维对沥青混合料韧性的影响,选用AC-13C和SMA-13两种混合料,使用UTM-25试验机对添加纤维前后的马歇尔试件进行间接拉伸试验;通过扫描电镜对纤维沥青混合料进行微观观测,分析纤维对混合料韧性的影响及增韧机理。试验结果表明:掺加玄武岩纤维对混合料的韧性指标有较大幅度提高,改善了混合料的抗开裂性能;纤维在沥青混合料中形成三维网状结构,且与沥青间有良好的粘结能力,使得裂纹扩展时的能量释放率减少从而延缓裂缝的扩展。

关键词：玄武岩纤维韧性抗开裂性能间接拉伸微观观测机理分析马歇尔试件沥青混合料