检索结果-南通市图书馆

Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple

引用

Horticultural Plant Journal 2024年第3期10卷 629-640页

作者： Qinglong Dong Yi Tian Xuemei zhang Dingyue Duan He zhang Kaiyu Yang Peng Jia Haoan Luan Suping Guo Guohui Qi Ke mao Fengwang Ma College of Forestry Hebei Agricultural UniversityBaodingHebei 071001China National Engineering Research Center for Agriculture in Northern Mountainous Areas Agricultural Technology Innovation Center in Mountainous Areas of Hebei ProvinceHebei Agricultural UniversityBaodingHebei 071001China State Key Laboratory of Crop Stress Biology for Arid Areas Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China College of Horticulture Hebei Agricultural UniversityBaodingHebei 071001China

Abiotic stress reduces plant yield and *** transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain ***,we functionally characterized the apple GroupⅢWRKY gene ***-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic *** activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic *** localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal *** analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic *** affinity purification sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene *** binding was further verified by an electrophoretic mobility shift ***,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple.

关键词： Apple MdWRKY115 Osmotic stress Drought stress Function analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Full-length transcriptome sequencing analysis reveals differential skin color regulation in snakeheads fish Channa argus

引用

Aquaculture and Fisheries 2024年第4期9卷 590-596页

作者： Leigang mao Yongan Zhu Jiaren Yan Longgang zhang Shuren Zhu Li An Qinglei Meng Zhishan zhang Xirong Wang College of Fisheries and Life Science Shanghai Ocean UniversityShanghai201306China Shandong Freshwater Fisheries Research Institute Jinan250017China

Albinism is a genetic disorder caused by a series of genetic abnormalities that result in a decrease in *** yellow snakehead fish(GCAS)has been discovered while the Channa argus(CAS)breeding,but the genetic mechanism that causes its albinism is not *** sequencing and comparison of CAS and GCAS skin using Oxford Nanopore Technologies(ONT)technology to elucidate the molecular mechanisms of snakehead albinism.27.1 G clean reads and 26,198 full-length non-redundant sequences were generated via ONT ***,8079 new transcripts and 7231 genes were identified by comparing and analyzing redundant removed transcripts and known reference genome *** results of deGs analysis showed that a total of 59 transcripts were differentially expressed in the two groups,including 22 up-regulated transcripts and 37 down-regulated *** causative genes associated with albinism have been identified,including TYR(Tyrosinase)and SOX10(SRY-box transcription factor 10)(tyrosinase synthesis and metabolism),S-100(S100 calcium binding protein A1)(melanin production),NLRC3(NLR family CARD domain containing 3)(disease immunity)are significantly down-regulated in GCAS,while RhoGEFs expression(melanin synthesis)is *** interaction analysis revealed that GAPDH(Glyceraldehyde-3-phosphate dehydrogenase)is the top hub gene in the regulation of skin color in *** addition,the skin of snakehead fish is regulated by metabolic(GAPDH),immune[Tumor protein p53(TP53),C-X-C motif chemokine ligand 8(CXCL8),SRC proto-oncogene,non-receptor tyrosine kinase(SRC),CD274]and neural[Notch receptor 1(NOTCH1),C-X-C motif chemokine ligand 12(CXCL12)]related genes to achieve differences in skin *** findings in understanding the process by which albinism in fish develops.

关键词： Albinism Channa argus Nanopore-based RNA sequencing Melanin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bazi Bushen alleviates reproductive aging in aged male mice

引用

Chinese Journal of Natural Medicines 2024年第5期22卷 416-425页

作者： MA Kun LI Mengnan SONG Yahui mao Xinjing zhang Shaolan zhang Yaping JIANG Yuning JI Chuanyuan NIU Kunxu LI Hongrong HOU Yunlong WEI Cong Graduate School Hebei University of Chinese MedicineShijiazhuang 050091China State Key Laboratory for Innovation and Transformation of Luobing Theory Shijiazhuang 050035China Key Laboratory of State Administration of TCM(Cardio-Cerebral Vessel Collateral Disease) Shijiazhuang 050035China College of Traditional Chinese Medicine·College of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing 210023China Hebei Provincial Key Laboratory of Luobing Shijiazhuang 050035China Key Disciplines of State Administration of TCM for Luobing Shijiazhuang 050035China

Bazi Bushen(BZBS),a traditional Chinese medicine(TCM),has demonstrated therapeutic efficacy in testicular dysfunction within D-galactose and NaNO_(2)mouse *** study aimed to ascertain if BZBS could also mitigate the decline in testicular function associated with natural ***,male aged mice were employed to evaluate the preventive effects of BZBS on male reproductive *** was achieved by assessing sex hormone production,testicular histomorphology,and *** to the untreated aged control group,BZBS administration elevated the levels of sex hormones and spermatocyte populations and preserved normal testicular structure in aged ***,spermatogenesis was *** analyses,including malondialdehyde(MDA)assays and real-time PCR,indicated that BZBS diminished testicular oxidative stress and the inflammatory *** these findings,mice treated with BZBS exhibited reductions in the populations of senescent and apoptotic cells within the seminiferous tubules,suggesting alleviated cellular *** contrast,we observed that rapamycin,a drug known for its longevity benefits,induced excessive testicular apoptosis and did not decrease lipid ***,our results highlight BZBS’s promising clinical potential in counteracting male reproductive aging,underlining its mechanisms of action.

关键词： Traditional Chinese medicine Male reproductive aging Spermatogenic cells Cell senescence Apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Author correction to“Immune-awakening Saccharomyces-inspired nanocarrier for oral target delivery to lymph and tumors”[Acta Pharmaceutica Sinica B 12(2022)4501-4518]

引用

Acta Pharmaceutica Sinica B 2024年第3期14卷 1478-1482页

作者： Yuling mao Xiudan Wang Caishun Chen Qinfu Zhao Yanfeng Liu Jinghai zhang Siling Wang department of Pharmaceutics School of PharmacyShenyang Pharmaceutical University103 Wenhua RoadShenyang110016China department of Biomedical Engineering School of Medical DevicesShenyang Pharmaceutical University103 Wenhua RoadShenyang110016China School of Life Science and Bio-pharmaceutics Shenyang Pharmaceutical University103 Wenhua RoadShenyang110016China

The authors regret that there is an error in the article Fig.9D1 due to the mistake of copying and pasting in the process of assembling figures and negligence in the proofreading,and also a mislabled error in the supp... 详细信息

关键词： correction supporting proof

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An octopamine-specific GRAB sensor reveals a monoamine relay circuitry that boosts aversive learning

引用

National Science Review 2024年第5期11卷 186-201页

作者： Mingyue Lv Ruyi Cai Renzimo zhang Xiju Xia Xuelin Li Yipan Wang Huan Wang Jianzhi Zeng Yifei Xue Lanqun mao Yulong Li State Key Laboratory of Membrane Biology School of Life SciencesPeking University State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences PKU-IDG/Mc Govern Institute for Brain Research Yuanpei College Peking University Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Peking University–Tsinghua University–National Institute of Biological Sciences Joint Graduate Program Academy for Advanced Interdisciplinary Studies Peking University Institute of Molecular Physiology Shenzhen Bay Laboratory College of Chemistry Beijing Normal University Chinese Institute for Brain Research

Octopamine（OA）,analogous to norepinephrine in vertebrates,is an essential monoamine neurotransmitter in invertebrates that plays a significant role in various biological functions,including olfactory associative ***,the spatial and temporal dynamics of OA in vivo remain poorly understood due to limitations associated with the currently available methods used to detect *** overcome these limitations,we developed a genetically encoded GPCR activation-based（GRAB） OA sensor called *** sensor is highly selective for OA and exhibits a robust and rapid increase in fluorescence in response to extracellular *** GRABOA1.0,we monitored OA release in the Drosophila mushroom body（MB）,the fly's learning center,and found that OA is released in response to both odor and shock stimuli in an aversive learning *** OA release requires acetylcholine（ACh） released from Kenyon cells,signaling via nicotinic ACh ***,we discovered that OA amplifies aversive learning behavior by augmenting dopamine-mediated punishment signals via Octβ1R in dopaminergic neurons,leading to alterations in synaptic plasticity within the ***,our new GRABOA1.0sensor can be used to monitor OA release in real time under physiological conditions,providing valuable insights into the cellular and circuit mechanisms that underlie OA signaling.

关键词： octopamine dopamine GRAB sensor learning and memory

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Wafer-scale carbon-based CMOS PDK compatible with siliconbased VLSI design flow

引用

Nano Research 2024年第8期17卷 7557-7566页

作者： Minghui Yin Haitao Xu Yunxia You Ningfei Gao Weihua zhang Hongwei Liu Huanhuan Zhou Chen Wang Lian-mao Peng Zhiqiang Li Institute of Microelectronics Chinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China State Key Lab of Fabrication Technologies for Integrated Circuits Beijing 100029China Beijing Hua Tan Yuan Xin Electronics Technology Co. Ltd.Beijing 101399China Institute of Carbon-based Thin Film Electronics Peking UniversityShanxi 030012China Key Laboratory for the Physics and Chemistry of Nanodevices and Center for Carbon-based Electronics School of ElectronicsPeking UniversityBeijing 100871China

Carbon nanotube field-effect transistors(CNTFETs)are increasingly recognized as a viable option for creating high-performance,low-power,and densely integrated circuits(ICs).Advancements in carbon-based electronics,encompassing materials and device technology,have enabled the fabrication of circuits with over 1000 gates,marking carbon-based integrated circuit design as a burgeoning field of research.A critical challenge in the realm of carbon-based very-large-scale integration(VLSI)is the lack of suitable automated design methodologies and infrastructure *** this study,we present the development of a waferscale 3μm carbon-based complementary metal-oxide-semiconductor(CMOS)process design kit(PDK)(3μm-CNTFETs-PDK)compatible with silicon-based Electronic design Automation(EDA)tools and VLSI circuit design *** proposed 3μm-CNTFETs-PDK features a contacted gate pitch(CGP)of 21μm,a gate density of 128 gates/mm^(2),and a transistor density of 554 transistors/mm^(2),with an intrinsic gate delay around 134 *** of the 3μm-CNTFETs-PDK was achieved through the successful design and tape-out of 153 standard cells and 333-stage ring oscillator *** the carbon-based PDK and a silicon-based design platform,we successfully implemented a complete 64-bit static random-access memory(SRAM)circuit system for the first time,which exhibited timing,power,and area characteristics of clock@10 kHz,122.1μW,3795μm×2810μ*** research confirms that carbon-based IC design can be compatible with existing EDA tools and silicon-based VLSI design flow,thereby laying the groundwork for future carbon-based VLSI advancements.

关键词： carbon nanotube field-effect transistors(CNTFETs) complementary metal-oxide-semiconductor(CMOS) process design kit(PDK) wafer-scale very-large-scale integration(VLSI)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

RNF6 promotes chronic myelogenous leukemia cell proliferation and migration by stabilizing vimentin via multiple atypical ubiquitinations

引用

Genes & Diseases 2024年第1期11卷 87-90页

作者： Hongxia zhang Yueya Zhong Yuanming He Yujia Xu Ying Ren Haixia Zhuang Tong Sun Zhigang Zhu Xinliang mao Institute of Clinical Pharmacology Science and Technology Innovation CenterGuangzhou University of Chinese MedicineGuangzhouGuangdong 510403China Guangdong Provincial Key Laboratory of Protein Modification and degradation School of Basic Medical SciencesGuangzhou Medical UniversityGuangzhouGuangdong 511436China department of Pharmacology College of Pharmaceutical SciencesSoochow UniversitySuzhouJiangsu 215123China Division of Hematology&Oncology Department of GeriatricsGuangzhou First People's HospitalCollege of MedicineSouth China University of TechnologyGuangzhouGuangdong 510180China

Chronic myelogenous leukemia(CML)is a malignancy from bone marrow myeloid stem cells mainly driven by the fusion gene *** addition to BCR-ABL,other genes including RNF6 are also dysregulated in CML cells.1 RNF6,a ubiquitin ligase of the RING family,promotes various cancer cell proliferation,chemoresistance,and tumor growth in vivo by targeting various proteins for ubiquitination and degradation,including SHP1,TLE3,FOXA1,and MAD1.^(2) However,its specific mechanism in CML is not known.

关键词： ubiquitin myelogenous leukemia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The cluster digging behavior of larvae confers trophic benefits to fitness in insects

引用

Insect Science 2024年第3期31卷 870-884页

作者： Yujie Wu Qiang Wang Weikang Yang Sheng zhang Chuan-Xi mao Nana He Shaojie Zhou Chuanming Zhou Wei Liu School of Plant Protection Anhui Agricultural UniversityAnhui Province Key Laboratory of Crop Integrated Pest ManagementAnhui Province Engineering Laboratory for Green Pesticide Development and ApplicationHefeiChina College of Plant Protection Nanjing Agricultural UniversityNanjingChina School of Teacher Education Nanjng Xiaozhuang UniversityNanjingChina State Key Laboratory of Biocatalysis and Enzyme Engineering Hubei Province Key Laboratory of Biotechnology of Chinese Traditional MedicineNational&Local Joint Engineering Research Center of High-throughput Drug Screening TechnologySchool of Life ScienceHubei UniversityWuhanChina

Collective behaviors efficiently impart benefits to a diversity of species ranging from bacteria to *** larvae tend to cluster and form coordinated digging groups under crowded conditions,yet understanding the rules governing this behavior is in its *** primarily took advantage of the Drosophila model to investigate cooperative foraging ***,we report that Drosophila-related species and the black soldier fly have evolved a conserved strategy of cluster digging in food ***,we investigated relative factors,including larval stage,population density,and food stiffness and quality,that affect the cluster digging ***,oxygen supply through the posterior breathing spiracles is necessary for the organization of digging *** importantly,we theoretically devise a mathematical model to accurately calculate how the cluster digging behavior expands food resources by diving depth,cross-section area,and food *** found that cluster digging behavior approximately increases 2.2 fold depth,1.7-fold cross-section area,and 1.9 fold volume than control groups,*** of food sources significantly facilitates survival,larval development,and reproductive success of Drosophila challenged with competition for limited food resources,thereby conferring trophic benefits to fitness in ***,our findings highlight that the cluster digging behavior is a pivotal behavior for their adaptation to food scarcity,advancing a better understanding of how this cooperative behavior confers fitness benefits in the animal kingdom.

关键词： cluster digging determinant development fecundity larvae mathematical model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hypoxia activates the hypoxia-inducible factor-1α/vascular endothelial growth factor pathway in a prostatic stromal cell line:A mechanism for the pathogenesis of benign prostatic hyperplasia

引用

Current Urology 2024年第3期18卷 185-193页

作者： Tao zhang Changlin mao Yao Chang Jiaju Lyu delong Zhao Sentai Ding department of Urology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanChina department of Urology Mindong Hospital Affiliated to Fujian Medical UniversityFuanChina

Background The development of benign prostatic hyperplasia(BPH)is closely related to hypoxia in the prostatic stroma,and the hypoxia-inducible factor-1α/vascular endothelial growth factor(HIF-1α/VEGF)pathway has been shown to significantly activate in response to *** underlying mechanism for activation of this pathway in the pathogenesis of BPH remains *** and methods We constructed HIF-1αoverexpression and knockdown BPH stromal(WPMY-1)and epithelial(BPH-1)cell lines,which were cultured under different oxygen conditions(hypoxia,normoxia,and hypoxia+HIF-1αinhibitor).Quantitative real-time polymerase chain reaction(qPCR)and Western blotting were applied to detect the expression of the HIF-1α/VEGF *** proliferation and apoptosis were analyzed by Cell Counting Kit-8 and flow *** used the miRWalk 2.0 database and Western blotting to predict the potential miRNA that selectively targets the HIF-1α/VEGF pathway,and verified the prediction by qPCR and dual-luciferase *** In a BPH stromal cell line(WPMY-1),the expression of VEGF was in accordance with HIF-1αlevels,elevated in the overexpression cells and decreased in the knockdown ***-induced HIF-1αoverexpression,which could be reversed by a HIF-1α***,the HIF-1αinhibitor significantly depressed cellular proliferation and promoted apoptosis in hypoxic conditions,assessed by Cell Counting Kit-8 and flow ***,in the BPH epithelial cell line(BPH-1),the expression level of HIF-1αdid not influence the expression of ***,a potential miRNA,miR-17-5p,regulating the HIF-1α/VEGF pathway was predicted from the miRWalk 2.0 database and Western blotting,and verified by qPCR and dual-luciferase *** In hypoxia,activation of the HIF-1α/VEGF pathway plays a crucial role in regulating cell proliferation in a BPH stromal cell *** by miR-17-5p may be the potential mechanism for the activation of this *** of thi

关键词： Benign prostatic hyperplasia Hypoxia-inducible factor-1α Vascular endothelial growth factor Pathogenesis miR-17-5p

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Across two phylogeographic breaks: Quaternary evolutionary history of a mountain aspen (Populus rotundifolia) in the Hengduan Mountains

引用

Plant Diversity 2024年第3期46卷 321-332页

作者： Jieshi Tang Xiaoyan Fan Richard I.Milne Heng Yang Wenjing Tao Xinran zhang Mengyun Guo Jialiang Li Kangshan mao Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610065PR China Institute of Molecular Plant Sciences University of EdinburghEdinburgh EH93JHUK School of Ecology and Environment Tibet UniversityLhasa 850000PR China

Biogeographical barriers to gene flow are central to plant *** East Asia,plant distribution is greatly influenced by two phylogeographic breaks,the Mekong-Salween Divide and Tanaka-Kaiyong Line,however,few studies have investigated how these barriers affect the genetic diversity of species that are distributed across *** we used 14 microsatellite loci and four chloroplast DNA fragments to examine genetic diversity and distribution patterns of 49 populations of Populus rotundifolia,a species that spans both the Mekong-Salween Divide and the Tanaka-Kaiyong Line in southwestern *** and migration hypotheses were tested using coalescent-based *** historical gene flow was observed between the western and eastern groups of ***,but substantial flow occurred across both the Mekong-Salween Divide and Tanaka-Kaiyong Line,manifesting in clear admixture and high genetic diversity in the central ***-borne pollen and seeds may have facilitated the dispersal of *** following prevalent northwest winds in the *** also found that the Hengduan Mountains,where multiple genetic barriers were detected,acted on the whole as a barrier between the western and eastern groups of *** niche modeling suggested that *** has undergone range expansion since the last glacial maximum,and demographic reconstruction indicated an earlier population expansion around 600 *** phylogeographic pattern of *** reflects the interplay of biological traits,wind patterns,barriers,niche differentiation,and Quaternary climate *** study emphasizes the need for multiple lines of evidence in understanding the Quaternary evolution of plants in topographically complex areas.

关键词： Chloroplast DNA Microsatellite Phylogeographic break Populus rotundifolia Quaternary history Wind direction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：