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Machine Learning Approach to Enhance the Performance of MNP?Labeled Lateral Flow Immunoassay

Nano-Micro Letters 2019年第1期11卷 132-146页

作者： Wenqiang Yan Kan Wang Hao Xu Xuyang Huo Qinghui Jin daxiang cui Department of Instrument Science and Engineering School of Electronic Information and Electrical Engineering Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment InstrumentKey Laboratory of Thin Film and Microfabrication (Ministry of Education) Shanghai Jiao Tong University School of Naval Architecture Ocean and Civil Engineering Shanghai Jiao Tong University Department of Biomedical Engineering JiLin Medical University State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Faculty of Electrical Engineering and Computer Science Ningbo University

The use of magnetic nanoparticle(MNP)-labeled immunochromatography test strips(ICTSs) is very important for point-ofcare testing(POCT). However, common diagnostic methods cannot accurately analyze the weak magnetic signal from ICTSs, limiting the applications of POCT. In this study, an ultrasensitive multiplex biosensor was designed to overcome the limitations of capturing and normalization of the weak magnetic signal from MNPs on ICTSs. A machine learning model for sandwich assays was constructed and used to classify weakly positive and negative samples, which significantly enhanced the specificity and sensitivity. The potential clinical application was evaluated by detecting 50 human chorionic gonadotropin(HCG) samples and 59 myocardial infarction serum samples. The quantitative range for HCG was 1–1000 mIU mL^(-1) and the ideal detection limit was 0.014 mIU mL^(-1), which was well below the clinical threshold. Quantitative detection results of multiplex cardiac markers showed good linear correlations with standard values. The proposed multiplex assay can be readily adapted for identifying other biomolecules and also be used in other applications such as environmental monitoring, food analysis, and national security.

关键词： Point-of-care testing Immunochromatography test strips Magnetic nanoparticles Machine learning Support vector machine

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Dual-signal readout paper-based wearable biosensor with a 3D origami structure for multiplexed analyte detection in sweat

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Microsystems & Nanoengineering 2023年第2期9卷 157-166页

作者： Yuemeng Cheng Shaoqing Feng Qihong Ning Tangan Li Hao Xu Qingwen Sun daxiang cui Kan Wang School of Sensing Science and Engineering School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityKey Laboratory of Thin Film and Microfabrication Technology(Ministry of Education)200240 ShanghaiChina Department of Plastic and Reconstructive Surgery Shanghai Ninth People's HospitalShanghai JiaoTong University School of Medicine200011 ShanghaiChina School of Naval Architecture Ocean&Civil EngineeringShanghai Jiao Tong University200240 ShanghaiChina

In this research,we design and implement a small,convenient,and noninvasive paper-based microfluidic sweat sensor that can simultaneously detect multiple key biomarkers in human sweat.The origami structure of the chip includes colorimetric and electrochemical sensing regions.Different colorimetric sensing regions are modified with specific chromogenic reagents to selectively identify glucose,lactate,uric acid,and magnesium ions in sweat,as well as the pH value.The regions of electrochemical sensing detect cortisol in sweat by molecular imprinting.The entire chip is composed of hydrophilically and hydrophobically treated filter paper,and 3D microfluidic channels are constructed by using folding paper.The thread-based channels formed after the hydrophilic and hydrophobic modifications are used to control the rate of sweat flow,which in turn can be used to control the sequence of reactions in the differently developing colored regions to ensure that signals of the best color can be captured simultaneously by the colorimetric sensing regions.Finally,the results of on-body experiments verify the reliability of the proposed sweat sensor and its potential for the noninvasive identification of a variety of sweat biomarkers.

关键词： Dual structure wear

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R11 peptides can promote the molecular imaging of spherical nucleic acids for bladder cancer margin identification

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Nano Research 2022年第3期15卷 2278-2287页

作者： Minghai Ma Pu Zhang Xiao Liang daxiang cui Qiuya Shao Haibao Zhang Mengzhao Zhang Tao Yang Lu Wang Nan Zhang Minxuan Jing Lu Zhang Weichao Dan Rundong Song Xi Liu Jiatao Hao Yuhang Chen Lijiang Gu Lei Wang Jinhai Fan Department of Urology The First Affiliated HospitalXi’an Jiaotong UniversityXi’an 710061China Department of Urology Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhou 310014China Department of Thoracic Surgery Tangdu HospitalAir Force Medical UniversityXi’an 710038China Department of Bio-Nano-Science and Engineering National Key Laboratory of Nano/Micro Fabrication TechnologyInstitute of Micro-Nano Science and TechnologyShanghai Jiao Tong UniversityShanghai 200240China

One of the critical problems in bladder cancer(BC)management is the local recurrence of disease.However,achieving the accurate delineation of tumor margins intraoperatively remains extremely difficult due to the lack of effective tumor margin recognition technology.Herein,survivin molecular beacon(MB)and R11 peptide-linked spherical nucleic acids(SNAs)were synthesized as nanoprobes(AuNP-MB@R11)for sensitive detection of BC margins.Physicochemical properties proved that R11 peptides and survivin MB were successfully loaded onto the surface of SNAs.AuNP-MB@R11 had good stability against nuclease activity and high sensitivity and specificity to detect survivin single strand DNA(ssDNA)in vitro.According to cytology,R11 peptides could increase the BC targeting ability and membrane penetrability of SNAs.Notably,R11 peptides significantly promoted the disintegration of lysosomes and the release of SNAs to enhance fluorescence imaging quality.Further RNA sequencing proved that some genes and pathways related to endocytosis and lysosomes were significantly regulated,such as AGPAT5,GPD1L,and GRB2.In orthotopic BC models and a clinical sample from a patient with BC,AuNP-MB@R11 showed a more legible cancerous fluorescence margin and offered remarkably improved detection compared to those achieved by SNAs.R11 peptide-linked SNAs present promising potential to identify BC margin,which may help to improve the R0 resection rate in surgery and improve patients’quality of life.

关键词： R11 peptides molecular beacons spherical nucleic acids tumor targeting lysosome escape bladder cancer

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Potassium sodium tartrate-assisted hydrothermal synthesis of BaLuFs ：Yb3＋/Er3＋ nanocrystals

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Particuology 2016年第1期14卷 164-169页

作者： Rongjin Sun Peiyu Qiu Ting Yin Guo Gao Hualin Fu Kan Wang Chunlei Zhang daxiang cui Institute of Nano Biomedicine and Engineering Department of Instrument Science and Technology Key Laboratory for Thin Film and MicrofabricationTechnology of Ministry of Education School of Electronic Information and Electrical Engineering School of Materials Science and Engineering Shanghai JiaoTong University Shanghai 200240 China

Highly-dispersed BaLuFs：yb3＋/Er3＋ nanocrystals were prepared by a facile potassium sodium tartrate- assisted hydrothermal method. The average particle size was approximately 20-25 nm. The formation mechanism is discussed. Potassium sodium tartrate led to form a complex with an approximately three- dimensional network structure, which insured largely concurrent nucleation. As a result, we acquired uniform nanoparticles. The hydrothermal temperature, holding time, and pH value were important fac- tors affecting the formation of the BaLuF5 ：yb3＋/Er3＋ nanocrystals. We investigated their influence on the formation and realized the optimal reaction parameters. Remarkably, potassium sodium tartrate also con- tributed to the biocompatibility and potential biomedical applications of BaLuFs ：Yb3＋/Er3＋ nanocrystals by decomposing into small organic groups attached to the nanoparticles.

关键词： Upconversion BaLuFs PST Hydrothermal synthesis

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One Step Quick Detection of Cancer Cell Surface Marker by Integrated NiFe-based Magnetic Biosensing Cell Cultural Chip

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Nano-Micro Letters 2013年第3期5卷 213-222页

作者： Chenchen Bao Lei Chen Tao Wang Chong Lei Furong Tian daxiang cui Yong Zhou Key Laboratory for Thin Film and Microfabrication of Ministry of Education Research Institute of Micro/Nano Science and TechnologyShanghai Jiao Tong University FOCAS Research Institute Dublin Institute of Technology

RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a specially designed NiF e-based magnetic biosensing cell chip combined with functionalized magnetic nanoparticles. Magnetic nanoparticles with 20-30 nm in diameter were prepared by coprecipitation and modified with RGD-4C, and the resultant RGD-functionalized magnetic nanoparticles were used for targeting cancer cells cultured on the NiF e-based magnetic biosensing chip and distinguish the amount of cell surface receptor-integrin.Cell lines such as Calu3, Hela, A549, CaF br, HEK293 and HUVEC exhibiting different integrin expression were chosen as test samples. Calu3, Hela, HEK293 and HUVEC cells were successfully identified. This approach has advantages in the qualitative screening test. Compared with traditional method, it is fast, sensitive, low cost,easy-operative, and needs very little human intervention. The novel method has great potential in applications such as fast clinical cell surface marker detection, and diagnosis of early cancer, and can be easily extended to other biomedical applications based on molecular recognition.

关键词： Magnetic impedance Cancer cell Targeted detection Biosensing system Cell cultural chip

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N-doped coaxial CNTs@a-Fe_2O_3@C nanofibers as anode material for high performance lithium ion battery

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Journal of Energy Chemistry 2018年第5期27卷 1453-1460页

作者： Peng Huang Wei Tao Haixia Wu Xiaogang Li Ting Yin Qian Zhang Wen Qi Guo Gao daxiang cui Institute of Nano Biomedicine and Engineering. Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument Department of Instrument Science and Engineering. School of Electronic Information and Electrical Engineering. Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 China College of Chemistry and Bioengineering Yichun University Yichun 336000 Jiangxi China

N-doped coaxial CNTs@α-Fe_2O_3@C nanofibers have been successfully synthesized according to a facile solvothermal/hydrothermal method. The obtained CNTs@α-Fe_2O_3@C nanofibers composites exhibited spe- cial three-dimensional （3-D） network structure, which endows they promising candidate for anode ma- terials of lithium ion battery. The coaxial property of CNTs@α-Fe_2O_3@C nanofibers could significantly improve the cycling and rate performance owing to the acceleration of charge/electron transfer, improve- ment of conductivity, maintaining of structural integrity and inhibiting the aggregation. The α-Fe_2O_3 nanoparticles with small size and high percentage of N-doped amount could further improve the elec- trochemical performance. As for the CNTs@α-Fe_2O_3@C nanofibers, the capacity presented a high value of 1255.4 mAh/g at 0.1 C, and retained at 1213.4 mAh/g after 60 cycles. Even at high rate of 5 C, the ca- pacity still exhibited as high as 319 mAh/g. The results indicated that the synthesized N-doped coaxial CNTs@α-Fe_2O_3@C nanofibers exhibited high cvcling and rate oerformance.

关键词： N-doped Coaxial nanofibers Lithium batteries α-Fe_2O_3

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Remote Tracking Gas Molecular via the Standalone-Like Nanosensor-Based Tele-Monitoring System

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Nano-Micro Letters 2021年第2期13卷 207-220页

作者： Han Jin Junkan Yu daxiang cui Shan Gao Hao Yang Xiaowei Zhang Changzhou Hua Shengsheng cui cuili Xue Yuna Zhang Yuan Zhou Bin Liu Wenfeng Shen Shengwei Deng Wanlung Kam Waifung Cheung Institute of Micro-Nano Science and Technology School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China National Engineering Research Center for Nanotechnology Shanghai 200240People’s Republic of China School of Electrical Engineering and Computer Science Ningbo UniversityNingbo 315211People’s Republic of China State Key Laboratory of Pathogen and Biosecurity Institute of Microbiology and EpidemiologyAcademy of Military Medical SciencesBeijing 100071People’s Republic of China Ningbo Materials Science and Technology Institute Chinese Academy of SciencesNingbo 315201People’s Republic of China College of Chemical Engineering Zhejiang University of TechnologyHangzhou 310014People’s Republic of China Qi Diagnostics Ltd HongkongPeople’s Republic of China

Remote tracking the variation of air quality in an effective way will be highly helpful to decrease the health risk of human short-and long-term exposures to air pollution.However,high power consumption and poor sensing performance remain the concerned issues,thereby limiting the scale-up in deploying air quality tracking networks.Herein,we report a standalone-like smart device that can remotely track the variation of air pollutants in a power-saving way.Brevity,the created smart device demonstrated satisfactory selectivity(against six kinds of representative exhaust gases or air pollutants),desirable response magnitude(164–100 ppm),and acceptable response/recovery rate(52.0/50.5 s),as well as linear response relationship to NO2.After aging for 2 weeks,the created device exhibited relatively stable sensing performance more than 3 months.Moreover,a photoluminescence-enhanced light fidelity(Li-Fi)telecommunication technique is proposed and the Li-Fi communication distance is significantly extended.Conclusively,our reported standalone-like smart device would sever as a powerful sensing platform to construct high-performance and low-power consumption air quality wireless sensor networks and to prevent air pollutant-induced diseases via a more effective and low-cost approach.

关键词： Metal-organic framework-derived polyhedral ZnO Perovskite quantum dots Nanosensor NO2 Tele-monitoring system

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Human iPS Cells Loaded with MnO2-Based Nanoprobes for Photodynamic and Simultaneous Enhanced Immunotherapy Against Cancer

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Nano-Micro Letters 2020年第10期12卷 36-52页

作者： Yanlei Liu Jingxing Yang Bin Liu Wen Cao Jingpu Zhang Yuming Yang Lijun Ma Jesus Martinez de la Fuente Jie Song Jian Ni Chunfu Zhang daxiang cui Institute of Nano Biomedicine and Engineering Key Laboratory for Thin Film and Microfabrication of Ministration of EducationShanghai Engineering Research Centre for Intelligent Diagnosis and Treatment InstrumentDepartment of Instrument Science and EngineeringSchool of Electronic Information and Electrical EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240People’s Republic of China National Engineering Center for Nanotechnology Shanghai 200240People’s Republic of China Department of Nuclear Medicine Rui Jin HospitalSchool of Biomedical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China Scientific Research Center Shanghai Public Health Clinical CenterFudan UniversityShanghai 201508People’s Republic of China Tongren Hospital Shanghai Jiao Tong University School of Medicine1111 XianXia RoadShanghai 200336People’s Republic of China

How to trigger strong anti-tumor immune responses has become a focus for tumor therapy.Here,we report the human-induced pluripotent stem cells(iPSs)to deliver MnO2@Ce6 nanoprobes into tumors for simultaneous photodynamic therapy(PDT)and enhanced immunotherapy.Ce6 photosensitizer was attached on manganese dioxide(MnO2)nanoparticles,and resultant MnO2@Ce6 nanoprobes were delivered into mitomycin-treated iPSs to form iPS-MnO2@Ce6 nanoprobes.The iPS-MnO2@Ce6 actively targeted in vivo tumors,the acidic microenvironment triggered interaction between MnO2 and H2O2,released large quantities of oxygen,alleviated hypoxia in tumor.Upon PDT,singlet oxygen formed,broken iPSs released tumor-shared antigens,which evoked an intensive innate and adaptive immune response against the tumor,improving dendritic cells matured,effector T cells,and natural killer cells were activated.Meanwhile,regulatory T cells were reduced,and then the immune response induced by iPS-MnO2@Ce6 was markedly stronger than the immune reaction induced by MnO2@Ce6(P<0.05).The iPS-MnO2@Ce6 markedly inhibited tumor growth and metastasis and reduced mortality in mice models with tumor.Human iPS s loaded with MnO2-based nanoprobes are a promising strategy for simultaneous PDT and enhanced immunotherapy against tumor and own clinical translational prospect.

关键词： Human iPS MnO2@Ce6 nanoprobes Photodynamic therapy Immunotherapy Cancer

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Gold nanoprisms as a hybrid in vivo cancer theranostic platform for in situ photoacoustic imaging, angiography, and localized hyperthermia

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Nano Research 2016年第4期9卷 1043-1056页

作者： Chenchen Bao Joao Conde Fei Pan Chao Li Chunlei Zhang Furong Tian Shujing Liang Jesus M. de la Fuente daxiang cui Institute of Nano Biomedicine and Engineering Department of Instrument Science and Engineering School of Electronic Information and Electrical Engineering National Center for TranslationaI Medicine Collaborative Innovational Center for System Biology Shanghai Jiao Tong University Shanghai200240 China Massachusetts Institute of Technology Institute for Medical Engineering and Science Harvard-MIT Division for Health Sciences and Technology E25-449 Cambridge Massachusetts USA School of Engineering and Materials Science Queen Mary University of London E14N5 London UK School of Food Science and Environmental Health College of Science and Health Dublin Institute of Technology Camden Row 4 Dublin Ireland Instituto de Nanociencia de Aragon (INA) Universidad de Zaragoza Zaragoza 50018 Spain

The development of high-resolution nanosized photoacoustic contrast agents is an exciting yet challenging technological advance. Herein, antibody （breast cancer-associated antigen I （Brcaal） monoclonal antibody）- and peptide （RGD）- functionalized gold nanoprisms （AuNprs） were used as a combinatorial methodology for in situ photoacoustic imaging, angiography, and localized hyperthermia using orthotopic and subcutaneous murine gastric carcinoma models. RGD-conjugated PEGylated AuNprs are available for tumor angiography, and Brcaal monodonal antibody-conjugated PEGylated AuNprs are used for targeting and for in situ imaging of gastric carcinoma in orthotopic tumor models. In situ photoacoustic imaging allowed for anatomical and functional imaging at the tumor site. In vivo tumor angiography imaging showed enhancement of the photoacoustic signal in a time-dependent manner. Furthermore, photoacoustic imaging demonstrated that tumor vessels were clearly damaged after localized hyperthermia. This is the first proof-of-concept using two AuNprs probes as highly sensitive contrasts and therapeutic agents for in situ tumor detection and inhibition. These smart antibody/peptide AuNprs can be used as an efficient nanotheranostic platform for in vivo tumor detection with high sensitivity, as well as for tumor targeting therapy which, with a single-dose injection, results in tumor size reduction and increases mice survival after localized hyperthermia treatment.

关键词： gold nanoprisms,in situ gastric cancer,photoacoustic imaging,photothermal therapy

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GSH-triggered sequential catalysis for tumor imaging and eradication based on star-like Au/Pt enzyme carrier system

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Nano Research 2020年第1期13卷 160-172页

作者： Amin Zhang Qian Zhang Gabriel Alfranca Shaojun Pan Zhicheng Huang Jin Cheng Qiang Ma Jie Song Yunxiang Pan Jian Ni Lijun Ma daxiang cui Institute of Nano Biomedicine and Engineering Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment InstrumentDepartment of Instrument Science and EngineeringSchool of Electronic Information and Electrical EngineeringShanghai Jiao Tong University800 Dongchuan RDShanghai200240China National Center for Translational Medicine Collaborative Innovational Center for System BiologyShanghai Jiao Tong University800 Dongchuan RoadShanghai200240China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai200240China Instituto de Ciencia de Materiales de Aragón(ICMA) CSIC/Universidad de ZaragozaC/Pedro Cerbuna 1250009ZaragozaSpain Department of Tumor Xintai People’s HospitalXintai271200China Department of Tumor in Tongren Hospital Shanghai Jiao Tong University School of Medicine1111 XianXia RoadShanghai200336China

Distinctively different metabolism between tumor cells and normal cells endows tumor tissues unique microenvironment.In this regard,we have successfully prepared a sequential catalytic platform based on Au/Pt star for tumor theragnostic.The multifunctional probes consisted of a gold/platinum star-shaped core(Au/Pt star)conjugated with a GSH-sensitive disulfide bond(S–S),a targeting ligand(rHSA-FA),a near-infrared fluorophore(IR780)and glucose oxidase(GOx).When systemically administered in a xenografted murine model,the probes specifically targeted the tumor sites.As the disulfide linker was cleaved by intracellular GSH,the IR780 molecules could be released for photo-thermal therapy&photodynamic therapy(PTT&PDT)and imaging.Subsequently,the Pt nanolayer of the Au/Pt star and the GOx formed a sequential catalytic system:GOx effectively catalyzed intracellular glucose by consuming oxygen to generate H2O2 and enhance the local acidity,and the Pt layer exhibited peroxidase-like property to catalyze H2O2 producing toxic·OH for tumor oxidative damage.Here we demonstrated that our probes simultaneously possessed a GSH-sensitive release,real-time imaging ability,and synergetic cancer starving-like therapy/enzyme oxidative therapy/PTT/PDT features,which provides a potential strategy for effective tumor theragnostic.

关键词： Au/Pt star GSH-responsive GOx peroxidase sequential nanocatalyst cancer starving-like therapy enzyme oxidative therapy photo-thermal therapy&photodynamic therapy(PTT&PDT)

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