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Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics

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Asian Journal of Andrology 2012年第6期14卷 890-896页

作者： Ina O Specht Marcello Spano Karin S Hougaard Gian C Manicardi davide bizzaro Gunnar Toft Aleksander Giwercman Jens-Peter E Bonde Department of Occupational and Environmental Medicine University Hospital of Copenhagen Copenhagen NV DK-2400 Denmark Laboratory of Toxicology Unit of Radiation Biology and Human Health ENEA Casaccia Research Center Rome 1-00123 Italy National Research Centre for the Working Environment Copenhagen 0 DK-2100 Denmark Dipartimento di Scienze Agrarie e degli Alimenti Universita di Modena e Reggio Emilia Reggio Emilia 1-42121 Italy Department of Life and Environmental Sciences Polytechnic University of Marche Ancona 1-60131 Italy epartment of Occupational Medicine Aarhus University Hospital Aarhus C DK-8000 Denmark Reproductive Medicine Centre Maim6 University Hospital Lund University MalmO SE-20502 Sweden

Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife＇s antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay （SCSA） and the terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count （P〈O.01）, more motile sperms （P=-O.04） and fewer sperm head defects （P=-O.05）. These associations were consistent within and across study regions. Furthermore, testosterone, follicle-stimulating hormone （FSH） and sexual hormone-binding globulin （SHBG） were significantly negatively correlated with Fas within and across regions as well. The data indicated no association between the anti-apoptotic Bcl-xL marker and semen or personal characteristics. The finding of Fas-positive sperm cells associated with better semen quality in a cohort of spouses of pregnant women seems different from previous data obtained in infertile men and warrants further investigation to clarify the biological significance of sperm apoptotic markers.

关键词： apoptosis Bcl-x protein Fas-associated death domain protein fertility sperm chromatin structure assay (SCSA) sper- matozoa TUN EL assay

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