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提高细胞融合率和获得杂交瘤细胞过量生长的有效方法

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细胞与分子免疫学杂志 1988年第1期 42-46页

作者：李健强,david t.shen,WilliamC.Davis 西北农业大学兽医系微生物传染病组美国华圣顿州立大学美国华圣顿州立大学兽医学院教授美国农业部动物疾病研究单位微生物学家免疫学教授

B淋巴细胞促分裂素—鼠伤寒沙门氏菌(Salmonella typhimurium)细胞壁水溶性蛋白提取物(StM)的应用和延迟使用含HAt的选择性培养基等条件的改进,使细胞融合率提高到90％以上,分泌特异性抗体的杂交瘤细胞系阳性率提高到50％以上,并得到不... 详细信息

B淋巴细胞促分裂素—鼠伤寒沙门氏菌(Salmonella typhimurium)细胞壁水溶性蛋白提取物(StM)的应用和延迟使用含HAt的选择性培养基等条件的改进,使细胞融合率提高到90％以上,分泌特异性抗体的杂交瘤细胞系阳性率提高到50％以上,并得到不同类和亚类的单克隆抗体。某些影响杂交瘤细胞生长的因素和条件也作了讨论。

关键词：细胞 StM 培养液促分裂素鼠伤寒沙门氏菌完全培养基单克隆抗体融合率

COMPARISON OF tHREE MEtHODS tO PREDICt ADSORPtION ISOtHERMS FOR ORGANIC VAPORS FROM SIMILAR AND NON-SIMILAR POLARItY REFERENCE VAPORS

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Chinese Journal of Chemical Engineering 1989年第2期 298-308页

作者：汪大翚 t.shen Kenneth E.Noll Department of Chemical Engineering Zhejiang University Hangzhou Department of Environmental Engineering Illinois Institute of Techmology Chicago IL 60616 U.S.A.

the Polanyi-Dubinin equation was used to predict adsorption isotherms for eight organic vapors at 25℃ and for two organic vapors at 25℃, 40℃ and 60℃ on activated carbon in this analysis. the theoretical affinity coefficient, β, was calculated by three methods (molar volume, molecular parachor and electronic polarization) based on nonpolar and polar reference vapors.

关键词： isotherms affinity molar Polar deviation polar monitored adsorbed maintained beads

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活性炭上吸附平衡的三种预测方法比较

化工学报 1989年第6期40卷 655-663页

作者：汪大翚 t.shen Kenneth E.Noll 浙江大学化工系伊里诺斯理工学院环境工程系

在用三种方法(摩尔体积、分子等张比容及摩尔电子极化度)计算亲和系数β的基础上,用polanyi-Dubinin方程预计了25℃下八种有机蒸汽以及25、40和60℃下另两种有机蒸气在活性炭上的吸附等温线.相应的实验数据用重量法测定.预计的和实验的... 详细信息

在用三种方法(摩尔体积、分子等张比容及摩尔电子极化度)计算亲和系数β的基础上,用polanyi-Dubinin方程预计了25℃下八种有机蒸汽以及25、40和60℃下另两种有机蒸气在活性炭上的吸附等温线.相应的实验数据用重量法测定.预计的和实验的数据对比表明:(1)对于优化的预计,参照物应与被预计吸附质有相似的极性;(2)选择适宜的参照物后,三种预计方法在精度上无明显差异;(3)对不同温度下的预计,摩尔体积法更为简便、实用.

关键词：活性炭吸附平衡预测法

Fluorescence quenching of eosin upon adsorption by colloidal silver mediated in aqueous solution and reverse micelles

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Chinese Journal of Chemistry 1996年第4期14卷 315-320页

作者： Zang, L shen, t CHINESE ACAD SCI INST PHOTOG CHEMBEIJING 100101PEOPLES R CHINA

the optical effects of the adsorption of eosin on the colloidal sliver particle have been investigated in aqueous solutions. It was found that upon adsorption the fluorescence of eosin was effectively quenched. this was explained as the photoinduced interfacial electron transfer from the excited singlet state of eosin to the silver particle. Decreasing pH of the solution favors the adsorption of eosin and so enhances the fluorescence quenching. For comparison, the fluorescence quenching in reverse micelles was also investigated. the quenching behavior was much different from that mediated in aqueous solution. this was attributed to the unique microenvironment of reverse micelles.

关键词： photoinduced electron transfer eosin reverse micelle

23项生物产业研究动向值得关注 CNS亮点研究成果细盘点

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中国战略新兴产业 2016年第5期 32-37页

作者： t.shen

Cell、Nature和Science(CNS)是国际上代表生命科学最高水平的三大学术杂志。2015年三大杂志刊登了很多耐人寻味的研究,对于人类抗击癌症、艾滋病等医疗工作有着重要意义。编辑部摘编了一些研究成果亮点,希望从中一窥未来生物产业发展的... 详细信息

Cell、Nature和Science(CNS)是国际上代表生命科学最高水平的三大学术杂志。2015年三大杂志刊登了很多耐人寻味的研究,对于人类抗击癌症、艾滋病等医疗工作有着重要意义。编辑部摘编了一些研究成果亮点,希望从中一窥未来生物产业发展的新方向。

关键词：生物产业 Cell 造血干细胞脐带血骨髓细胞 HIV 星形细胞瘤重度抑郁症肿瘤复发候选疫苗衣壳蛋白研究者人类免疫缺陷病毒打印技术 Nature 胰腺肿瘤胰腺癌 CNS

Fully Distributed AC State Estimation Method for Power System Based on Information Propagation Algorithm

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Journal of Modern Power Systems and Clean Energy 2023年第4期11卷 1201-1211页

作者： Qiao Li Yu shen Lin Cheng david Wenzhong Gao University of Denver 2155 E Wesley AveDenverCO 80210USA tsinghua University BeijingChina

this paper proposes a new distributed AC state estimation *** from the popular distributed state estimation(DSE)methods based on area partitioning method,the proposed method is a truly distributed method in which the power system is not required to be divided into smaller areas and a centralized state estimator in each area is not *** order to achieve fully DSE,the information propagation algorithm is introduced in this paper to help the distributed local state estimators share the measurement *** information propagation algorithm is developed based on consensus *** proof of the convergence of the information propagation algorithm is provided in this ***,the AC state estimation method is integrated with the information propagation algorithm to realize the proposed *** proposed method is tested in different standard power system *** results show that the proposed method reaches the similar accuracy as the traditional centralized state estimation methods and performs faster and more accurate than the existing DSE methods.

关键词： State estimation consensus protocol distributed network graph theory

Late Ordovician Mass Extinction: Earth, fire and ice

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National Science Review 2024年第1期11卷 51-65页

作者： david A.t.Harper Palaeoecosystems Group Department of Earth SciencesDurham University

the Late Ordovician Mass Extinction was the earliest of the ‘big' five extinction events and the earliest to affect the trajectory of metazoan life. two phases have been identified near the start of the Hirnantian period and in the middle. It was a massive taxonomic extinction, a weak phylogenetic extinction and a relatively benign ecological extinction. A rapid cooling, triggering a major ice age that reduced the temperature of surface waters, prompted a drop in sea level of some 100 m and introduced toxic bottom waters onto the shelves. these symptoms of more fundamental planetary processes have been associated with a range of factors with an underlying driver identified as volcanicity. Volcanic eruptions, and other products, may have extended back in time to at least the Sandbian and early Katian, suggesting the extinctions were more protracted and influential than hitherto documented.

关键词： Ordovician LOME paleoecology palaeobiogeography biotas

Observation of Barrier-Induced Strain Relaxation in InGaAs/GaAs Single Quantum Wells

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Chinese Physics Letters 1994年第11期11卷 693-696页

作者： shen Wenzhong shen Xuechu tANG Wenguo t.Andersson National Laboratory for Infrared Physics Shanghai Institute of Technical PhysicsShanghai 200083 Department of Physics Chalmers University of Technology5-41296 GoteborgSweden

Resonably good agreement among the photoluminescence,absorption,in-plane photocurrent and theoretical calculation demonstrates the effect of GaAs barrier width on the strain in In_(0.20)Ga_(0.80)As/GaAs single quantum... 详细信息

关键词： InGaAs/GaAs Quantum Observation

Advances in dye-sensitized solar cell

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Chinese Science Bulletin 1997年第23期42卷 1937-1948页

作者： Jing, BW Zhang, MH shen, t Chinese Acad Sci Inst Photog Chem Beijing 100101 Peoples R China

tHE conversion of visible light to electricity using semiconductors as light absorbers has beenan important area of research for many years. In the 1970s, highly efficient photovoltaiccells based on silicon were developed (the light-to-electrical conversion efficiency≥25%),which provides energy to aircrafts. then semiconductor liquid-junction photoelectrochemicalcell started to be investigated. Generally speaking, highly efficient liquid-junction photoelec-trochemical solar cells can be obtained by using semiconductors such as Si, GaAs and WSe.(band gap≈(1.3±0.3) eV). Conversion efficiencies over 15% have been acquired on solarcells consisting of single crystals of Si or GaAs. But the serious photocorrosion and high

关键词： dye-sensitized solar cell ruthenium complex.