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Cu-DNAzyme facilitates highly sensitive immunoassay

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Chinese Chemical Letters 2019年第9期30卷 1652-1654页

作者： Zhi Shan Mingsheng Lyu Dennis curry david Oakley Ken Oakes Xu Zhang Verschuren Centre for Sustainability in Energy & the Environment Cape Breton University Sydney Now Scotia B1P 612 Canada College of Life Science Sichuan Agriculture University Yaan 625014 China Jiangsu Key Laboratory of Marine Bioresources and Environment Huaihai Institute of Technology Lianyungang 222005 China Department of Biology Cape Breton University Sydney Nova Scotia BJP 612 Canada Department of Chemistry Cape Breton University Sydney Nova Scotia B1P 612 Canada

Fast and sensitive antigen detection is important in biomedical research and development. Despite, being invented 48 years ago, the enzyme-linked immunosorbent assay(ELISA) remains one of the most successful and widely employed bioanalytical techniques in research and clinical diagnostics due to its reliability and simplistic design. Recently, nanotechnology has offered efficient signal reporting. In spite of some improvements in these systems, there are typically increased material costs involved and the need for expensive equipment or complicated chemical processes, thus negating any possible benefits over ELISA. Herein, we communicate a simple Cu-DNAzyme system for signal transduction of a CuO nanoparticle-labeled immunoassay. The reported immunoassay amplifies signal generation similar to traditional ELISA and is fast, simple, cost-effective, and sensitive, holding promise for biomedical applications and point-of-care testing.

关键词： Cu-DNAzyme Immunoassay ELISA CuO Nanoparticles

Amino acid digestibility in low-fat distillers dried grains with solubles fed to growing pigs

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Journal of Animal Science and Biotechnology 2014年第4期5卷 414-420页

作者： Shelby Marie curry Diego Mario david Labadan Navarro Ferdinando Nielsen Almeida Juliana Abranches Soares Almeida Hans Henrik Stein Department of Animal Sciences University of Illinois

The objective of this experiment was to determine the standardized ileal digestibility（SID） of amino acids（AA） in 3sources of distillers dried grains with solubles（DDGS） with different concentrations of *** growing barrows（initial body weight：76.1 ± 6.2 kg） were randomly allotted to a replicated 6×4 Youden square design with 6 diets and 4 *** fat content of the 3 sources of DDGS were 11.5,7.5,and 6.9%*** contained 60%DDGS and fat concentration of the diets were 7.5,52,and 5.2%,*** additional diets containing the 2sources of DDGS with 75 and 6.9%fat were also formulated,and corn oil was added to these diets to increase the concentration of fat in the diets to levels that were calculated to be similar to the diet containing conventional DDGS with 11.5%fat.A N-free diet was also formulated to calculate endogenous losses of crude protein（CP） and AA from the *** were fed experimental diets during four 7-d *** first 5 d of each period were an adaptation period and ileal digesta were collected on d 6 and 7 of each *** apparent ileal digestibililty（AID） and SID of CP and all indispensable AA,except AID Pro and SID of Trp,were greater（P 〈 0.01） in conventional DDGS than in the 2 sources of DDGS with reduced *** oil to the diets containing the 2 sources of DDGS with reduced fat did not consistently increase SID of *** conclusion,conventional DDGS has greater SID values for most AA compared with DDGS that contains less fat and inclusion of additional oil to diets containing low-fat DDGS does not increase AID or SID of *** lower AA digestibility in low-fat DDGS could not be overcome by the inclusion of additional fat to the diets.

关键词： Amino acid digestibility Distillers dried grains with solubles Pigs

Effect of Land Use Change on Carbon Content and CO<sub>2</sub>Flux of Cloud Forest Soils, Santa Elena, Costa Rica

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Open Journal of Soil Science 2014年第2期4卷 64-71页

作者： Lawrence H. Tanner david L. Smith Jessica curry Justin Twist Department of Biological Sciences Le Moyne College Syracuse USA Pottstown Memorial Medical Center Pottstown USA

We investigated the effects of land-use changes on soil carbon storage and soil CO2 flux by comparing soils from mature cloud forest and 31-year-old secondary forest, both in the Santa Elena Forest Reserve, a municipallyowned reserve at an elevation of 1600 to 1700 m near the town of Monteverde, and a clear-cut pasture near the reserve. Soils in the mature forest exhibit only weak horizonation but typically thick A horizons;they also consistently yield the highest carbon contents in the upper 30 cm. Soil CO2 flux was the highest in these soils, but also displayed the highest spatial variability. Secondary forest soils contain substantially less soil carbon than mature forest soils, but more than pasture soils. CO2 flux in the secondary forest soils was more similar to that of the mature forest, but displayed lower spatial variability. The pasture soils contain less soil carbon and produced lower CO2 flux levels than either of the forest soils. The pasture soils typically contain a well-defined coarse sandy layer 10 to 20 cm below the surface that we interpret as a sediment layer deposited across much of the landscape following a widespread erosion event, likely a consequence of the clear-cutting. Soil nitrogen concentrations are more than an order of magnitude lower than soil carbon concentrations, and display no trends between the different landscapes examined. Our preliminary results suggest that reforestation does restore soil carbon to clear-cut landscapes, but returning soil carbon levels to pre-land use levels occurs at a time scale of centuries, rather than decades.

关键词： Cloud Forest Secondary Succession Soil Carbon Soil Nitrogen Soil CO2 Flux

The First Pilot Epigenetic Type Improvement of Neuropsychiatric Symptoms in a Polymorphic Dopamine D2 (-DRD2/ANKK (Taq1A)), OPRM1 (A/G), DRD3 (C/T), and MAOA (4R) Compromised Preadolescence Male with Putative PANDAS/CANS: Positive Clinical Outcome with Precision-Guided DNA Testing and Pro-Dopamine Regulation (KB220) and Antibacterial Therapies

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Open Journal of Immunology 2024年第3期14卷 60-86页

作者： Kenneth Blum Igor Elman david Han Colin Hanna david Baron Ashim Gupta Shan Kazmi Jag Khalsa Debasis Bagchi Thomas McLaughlin Rajendra D. Badgaiyan Edward J. Modestino Drew Edwards Catherine A. Dennen Eric R. Braverman Abdalla Bowirrat Keerthy Sunder Kevin Murphy Nicole Jafari Foojan Zeine Paul R. Carney Mark S. Gold Kai-Uwe Lewandowski Alireza Sharafshah Aryeh R. Pollack Panayotis K. Thanos Division of Addiction Research & Education Center for Sports and Mental Health Western University of Health Sciences Pomona CA USA Department of Psychiatry Boonshoft School of Medicine Wright State University and Dayton VA Medical Centre Dayton OH USA Division of Nutrigenomics Victory Nutrition International Lederoch PA USA Institute of Psychology ELTE Etvs Lornd University Budapest Hungary Department of Psychiatry School of Medicine University of Vermont Burlington VT USA The Kenneth Blum Behavioral & Neurogenetic Institute LLC. Austin TX USA Department of Molecular Biology Adelson School of Medicine Ariel University Ariel Israel Division of Neuromodulation Research Karma Doctors & Karma TMS Palm Springs CA USA Division of Personalized Interventions Peak Logic Del Mar CA USA Division of Personalized Medicine Cross-Cultural Research & Educational Institute San Clemente CA USA Awareness Integration Institute San Clemente CA USA Division of Personalized Pain Therapy Center for Advanced Spine Care of Southern Arizona Tucson AZ USA Department of Psychiatry Harvard School of Medicine Cambridge MA USA Department of Management Science and Statistics University of Texas San Antonio TX USA Behavioral Neuropharmacology and Neuroimaging Laboratory Clinical Research Institute on Addictions Department of Pharmacology and Toxicology Jacobs School of Medicine and Biomedical Sciences University at Buffalo Buffalo NY USA Future Biologics Lawrenceville GA USA Department of Microbiology Immunology & Tropic Diseases School of Medicine Georgetown University Washington DC USA Department of Pharmaceutical Sciences College of Pharmacy Texas Southern University Houston TX USA Department of Psychiatry School of Medicine Case Western University Cleveland OH USA Brain and Behavior Laboratory Curry College Milton MA USA The Neurogenesis Project Jacksonville FL USA Department of Family Medicine Jefferson Health Northeast Philadelphia PA USA Departmen

Pediatric autoimmune neuropsychiatric disorders associated with or without streptococcal and other bacterial infections (PANDAS/CANS) are emerging as a featured pediatric disorder. Although there is some controversy regarding treatment approaches, especially related to the behavioral sequelae, we have hypothesized in other published work that it is characterized by the rapid onset of Reward Deficiency Syndrome (RDS) in children. We propose utilizing a multi-systems biological approach involving the coupling of genetic addiction risk testing and pro-dopamine regulation (KB220/POLYGEN®) to help induce “dopamine homeostasis” in patients with PANDAS, especially those with known DNA-induced hypodopaminergia. This case study examines a 12-year-old Caucasian male with no prior psychiatric issues who presented with a sudden onset of severe anxiety, depression, emotional liability, and suicidal ideation. The patient underwent genotyping and the genetic addiction risk score (GARS) testing, which revealed risk polymorphisms in the dopamine D2 (-DRD2/ANKK (Taq1A), OPRM1 (A/G), DRD3 (C/T), and MAOA (4R) genes. These polymorphisms have been linked to hypodopaminergia. The patient was subsequently placed on research ID-KB220ZPBMPOLY (POLYGEN®), and albeit the possibility of bias, based upon self and parental assessment, a marked rapid improvement in psychiatric symptoms was observed. In the second phase of treatment (102 days utilizing KB220), the patient received standard antibody testing, which was positive for Lyme. Antibacterial therapy started immediately, and KB220z was discontinued to provide a wash-out period. A monotonic trend analysis was performed on each outcome measure, and a consistently decreasing trend was observed utilizing antibacterial therapy. Our recommendation, albeit only one case, is to utilize and further research a combined therapeutic approach, involving precision-guided DNA testing and pro-dopamine regulation along with antibacterial therapy, as well as

关键词： PANDAS CANS Genetic Addiction Risk Testing (GARS) Pro-Dopamine Regulation Hypodopaminergia Polymorphisms Antibacterial Therapy Infections

Observation of Phase-Locked States in the Atlantic Multi-Decadal Oscillation(AMO)

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Atmospheric and Climate Sciences 2018年第3期8卷 344-354页

作者： david Holmes Douglass Department of Physics and Astronomy University of RochesterRochesterNYUSA

We report that the Atlantic Multi-Decadal Oscillation (AMO) shows the same phase-locked states of period 2 and 3 years that have been reported in many other climate indices. In addition, we find that the report by Muller, curry et al. of an oscillation in the AMO of 9.1 years is a misinterpretation of a maximum in the Fourier spectrum.

关键词： Climate Shift El Nino AMO Phase-Locked States

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《UNIX系统编程——基于SVR4》

Internet（共创软件） 2002年第10期 88-88页

作者： david A.curry 孙伟峰

众所周知，Unix系统编程简洁、功能强大、适应性强、更重要的是安全性好。在此给大家介绍一本经典Unix编程图书——《UNIx系统编程——基于SVR4》。

关键词：《UNIX系统编程——基于SVR4》 Unix系统系统编程书评

Nature:研究利用单细胞分析阐明癌症干细胞在脑癌中的关键角色

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现代生物医学进展 2017年第6期17卷 I0003-I0003页

作者： Itay Tirosh, Andrew S. Venteicher, Christine Hebert, Leah E. Escalante, Keren Yizhak, Jonathan M. Fisher, Christopher Rodman, Mariella G. Filbin, Cyril Neftel, Jackson Nyman, Benjamin Izar, Joshua M. Francis, Kenneth J. Livak, Dave Gennert, Rahul Satija, Todd R. Golub, Miguel N. Rivera, Gad Getz, Orit Rozenblatt-Rosen, Bradley E. Bernstein, Aviv Regev Mario L. Suv agrave Andrew S. Venteicher, Christine Hebert, Leah E. Escalante, Keren Yizhak, Mariella G. Filbin, Cyril Neftel, Niyati Desai, Christina C. Luo, Aanand A. Patel, Maristela L. Onozato, Nicolo Riggi, Ravindra Mylvaganam, A. John Iafrate, Matthew P. Frosch, Miguel N. Rivera, Gad Getz, Bradley E. Bernstein, david N. Louis Mario L. Suv agrave Andrew S. Venteicher, Anoop P. Patel, Brian V. Nahed, William T. curry, Robert L. Martuza Daniel P. Cahill Christopher Mount Michelle Monje Mariella G. Filbin Todd R. Golub Joshua M. Francis Todd R. Golub Aviv Regev Broad Institute of Harvard and MIT Cambridge Massachusetts 02142 USA Department of Pathology and Center for Cancer Research Massachusetts General Hospital and Harvard Medical School Boston Massachusetts 02114 USA Department of Neurosurgery Massachusetts General Hospital and Harvard Medical School Boston Massachusetts 02114 USA Departments of Neurology Neurosurgery Pediatrics and Pathology Stanford University School of Medicine Stanford California 94305 USA Department of Pediatric Oncology Dana-Farber Cancer Institute and Children’s Hospital Cancer Center Boston Massachusetts 02215 USA Department of Medical Oncology Dana-Farber Cancer Institute Boston Massachusetts 02215 USA Howard Hughes Medical Institute Koch Institute Department of Biology MIT Cambridge Massachusetts 02139 USA

近日。来自麻省总医院、博德研究所及哈佛大学的研究人员通过联合研究，在单细胞水平上对脑瘤基因组进行了分析。他们发现。癌症干细胞或许能够诱发少突神经胶质瘤的发生，少突神经胶质瘤是一种缓慢发展但却非常难以治愈的脑癌，相关研... 详细信息

近日。来自麻省总医院、博德研究所及哈佛大学的研究人员通过联合研究，在单细胞水平上对脑瘤基因组进行了分析。他们发现。癌症干细胞或许能够诱发少突神经胶质瘤的发生，少突神经胶质瘤是一种缓慢发展但却非常难以治愈的脑癌，相关研究刊登于Nature杂志上。同时研究者还首次在人类脑瘤样本中鉴别出了癌症干细胞及其分化的后代细胞。

关键词：干细胞单细胞分析癌症脑瘤神经胶质瘤键角利用研究人员