CRISPR-based genome editing systems have been successfully and effectively used in many ***,only a few studies have reported the comparison between CRISPR/Cas9 and CRISPR/Cpf1 systems in the whole-genome *** many web-...
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CRISPR-based genome editing systems have been successfully and effectively used in many ***,only a few studies have reported the comparison between CRISPR/Cas9 and CRISPR/Cpf1 systems in the whole-genome *** many web-based toolkits are available,there is still a shortage of comprehensive,user-friendly,and plant-specific CRISPR databases and desktop *** this study,we identified and analyzed the similarities and differences between CRISPR/Cas9 and CRISPR/Cpf1 systems by considering the abundance of proto-spacer adjacent motif(PAM)sites,the effects of GC content,optimal proto-spacer length,potential universality within the plant kingdom,PAM-rich region(parr)inhibiting ratio,and the effects of G-quadruplex(G-Q)*** this information,we built a comprehensive CRISPR database(including 138 plant genome data sources,***/pc/***),which provides search tools for the identification of CRISPR editing sites in both CRISPR/Cas9 and CRISPR/Cpf1 *** also developed a desktop software on the basis of the Perl/Tk tool,which facilitates and improves the detection and analysis of CRISPR editing sites at the whole-genome level on Linux and/or Windows ***,this study provides helpful data and software for easy selection and application of CRISPR-based genome editing systems in plants.
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