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Evaluation of the Marginal Adaptation in Metal Crowns Using CAD/CAM and Manual Wax Patterns

Microscopy Research 2015年第2期3卷 26-32页

作者： Alan Sepulveda-Rodríguez Felipe Guerrero-Martínez César Gaitan-Fonseca Takashi Komabayashi Enrique Reyes-Vela david masuoka Department of Stomatology Universidad Autónoma de Aguascalientes Aguascalientes Mexico Faculty of Dentistry Universidad Autónoma de San Luis Potosí San Luis Potosí Mexico Department of Endodontics West Virginia University Charleston USA

The aim of this study is to evaluate the maximum and minimum distances between the model and the cast crown of three techniques using Scanning Electron Microscopy (SEM). Three technique groups were used for this study: group A (control), traditional manual wax patterns;group B, dipping wax patterns;group C, resin patterns made with CAD/CAM. For each group, 10 samples were made using the same model, and then metal cast. Marginal accuracies of the samples were evaluated by performing gap measurements using SEM with a magnification of 1200× (minimum distance). The data were statistically analyzed using the one-way analysis of variance (ANOVA) at the 0.05 significance level. The average (standard deviation) of the minimum distance [μm] was 22.5 (12.1), 9.9 (4.3), and 14.7 (6.6), in groups A, B, and C, respectively. The average standard deviation of gap area [μm2] was 21667.2 (3476.4), 9906.4 (1512.1), and 16048.8 (8123). In the minimum distance comparison, groups A and B (p = 0.006) showed statistically significant results. In the gap area comparison, there was no statistical significance among groups A, B, and C (p = 0.174). The marginal adaptations of all three techniques were within a reported clinically acceptable range of margin.

关键词： Marginal Adaptation CAD/CAM Metal Caps Wax Pattern

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Bevel Tip Deformation in New and Used Dental Needles

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Microscopy Research 2015年第1期3卷 1-5页

作者： César Gaitán- Fonseca Denise Romo- Castillo Bernardino Cerda- Cristerna david masuoka Amaury Pozos- Guillén Stomatology Department Aguascalientes University Aguascalientes City Mexico Basic Sciences Laboratory Faculty of Dentistry San Luis Potosi University San Luis Potosi Mexico

Objectives: To evaluate quantitatively deformation of the angle of the horizontal plane of the bevel tip in new dental needles and in dental needles used in clinical procedures. Material and Methods: A cross-sectional study was performed with 360 dental needles from four different manufacturers. The needles were divided into four groups (90 per group): Group 1, Monoject;Group 2, DLP;Group 3, Badiject, and Group 4, Zeyco. The new and used needles were evaluated by scanning electron microscopy to evaluate quantitatively the deformation on the bevel. Results: All groups showed a significantly statistical difference between new needles and needles used for one or two injections

关键词： Bevel Tip Deformation Dentistry Needles

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Unilateral Impact of Altered Loading by Changing Teeth Height on the TMJ Fibrocartilage: Disc and Condyle of Wistar Rats

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Microscopy Research 2016年第2期4卷 20-31页

作者： Roxanne M. Olvera-Farias Jose Raymundo Cruz-Perez Rogelio Salinas-Gutierrez Jose Antonio Guerrero-Diaz de Leon Juan B. Kouri-Flores Raul Rosales-Ibañez david masuoka Ito Alma Lilian Guerrero Barrera Departamento de Morfología Universidad Autónoma de Aguascalientes Aguascalientes México Departamento de Infectómica y Patogénesis Molecular CINVESTAV-IPN Mexico City Mexico Departamento de Estadística Universidad Autónoma de Aguascalientes Aguascalientes México Departamento de Estomatología Universidad Autónoma de San Luis Potosí San Luis Potosi México Departamento de Estomatología Universidad Autónoma de Aguascalientes Aguascalientes México Laboratorio de Biología Celular y Tisular Departamento de Morfología Universidad Autónoma de Aguascalientes Aguascalientes México

Temporomandibular joint (TMJ) is sensitive to loading and mechanical stress that provokes morphological changes produced by the impact in the occlusal plane. Here, this impact is evaluated in TMJ articular disc and articular cartilage using an in vivo model of unilateral occlusal plane impact and by analysis of serial tissue sections stained with Hematoxylin-Eosin (H-E) or with Masson trichrome technique. Thus, six groups of 5 Wistar rats (200 - 250 g) are subjected to biomechanical dental stimulation by placing unilateral resin occlusal interference, or unilateral tooth wear made by upper left molars artificial mechanical devastation (1 control and 2 experimental groups for each treatment). Each treatment is evaluated two times at 1 and 15 days post-treatment. By H-E staining, control groups show chondrocytes arrangement as several cord cell groups in comparison with the experimental groups, which show an arrangement in one cord cell along of articular disc. However, this yields no significant difference (p < 0.05) in cell number between control and experimental groups. In contrast, in articular cartilage chondrocytes are random distributed along the superficial zone in control groups, while in experimental groups cell-free regions are observed in superficial zone. An image Blue hue analysis for trichrome stain is performed to quantify collagen;this shows a significant collagen decrease (p < 0.05) in almost all experimental groups compared with the controls. A degenerative process biomechanically induced by unilateral occlusal plane modification, causes cell and tissue changes on the TMJ structures that remain the degenerative changes observed in early osteoarthritis.

关键词： Articular Disc Articular Cartilage Dental Occlusion Collagen Temporomandibular Joint

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