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Molecular cloning and differential expression patterns of the regulatory subunit B' gene of PP2A in goldfish,Carassius auratus

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Science China(life Sciences) 2009年第8期52卷 724-732页

作者： li david WanCheng Key Laboratory of Protein Chemistry & Developmental Biology of Education Ministry of China College of Life SciencesHunan Normal University Department of Biochemistry and Molecular Biology College of MedicineUniversity of Nebraska Medical Center Department of Ophthalmology and Visual Sciences College of MedicineUniversity of Nebraska Medical Center

It is well established that the protein serine/threonine phosphatase 2A(PP2A) plays very important roles in many different cellular processes,including cell proliferation and differentiation,gene expression,neurotransmission,apoptosis,and ***2A consists of three heterogenic subunits:the scaffold subunit A,the catalytic subunit C,and the regulatory subunit *** both the scaffold and the catalytic subunits contain only two forms,at least four families of the regulatory subunits,B,B',B'',and B''' have been *** regulatory subunits from different families are encoded by different genes and bear other functions besides directing the specificity of *** study the functions of the regulatory subunits of PP2A in lower vertebrates,we have cloned the full-length cDNA sequence of the gene encoding the regulatory subunit B'δ of PP2A from gold fish,Carassius auratus using 3'-RACE and 5'-RACE cloning *** results revealed that the full-length B'δ cDNA contains 2415 bp and encodes a protein of 555 amino *** B'δ protein displays a very high level of sequence identity with the B'δ regulatory subunit from other species of *** its expression pattern,RT-PCR revealed that the highest level of mRNA was detected in brain,a less level detected in liver,spermary,ovary,kidney and gill,and the lowest level detected in the *** different developmental stages of gold fish,the highest level of mRNA expression was detected at the stages of two-cell,multiple-cell,blastula and gastrula,and a decreased level of B'δ mRNA was detected in other developmental *** the protein level,the highest expression level of B'δ protein was found in spermary,ovary,brain and heart,a less amount found in liver and the lowest level detected in kidney,gill and ***,B'δ protein was strongly expressed at the stages of two-cell,multiple-cell,blastula,gastrula,neurula,and optic vesicle,and then decreased at the stages of brain differentiatio

关键词： PP2A Protein dephosphorylation PP2A-B’δ regulatory subunit.

Peptide-based approaches to identify and characterize proteins that recognize histone post-translational modifications

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Chinese Chemical Letters 2018年第7期29卷 1051-1057页

作者： Jianwei lin Xiang david li Department of Chemistry The University of Hong Kong

Increasing amount of evidence suggests that post-translational modifications(PTMs) on histones are involved in regulating DNA-associated processes such as gene expression and DNA-damage repair. To identify and characterize proteins that recognize histone PTMs, tools relying on synthetic peptides have been constructed and widely used in recent years. In this review, we first summarize the development and applications of these tools, which includes peptide-based pull-down assay and peptide-array-based high-throughput screening. The limitation of peptide-based approaches is then discussed, followed by a brief description on recent development of nucleosome-based tools.

关键词： Histone PTMs Peptide pull-down Crosslinkers Peptide microarrays Peptide libraries

Aortic valve leaflet disruption techniques in transcatheter aortic valve replacement

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Journal of Geriatric Cardiology 2022年第12期19卷 990-994页

作者： Brian Shin david li Hong liU Department of Anesthesiology and Pain Medicine University of California Davis HealthSacramentoUSA

With continued technological advancement and technical improvement of transcatheter aortic valve replacement(TAVR),it has become a desirable treatment option for aortic valve *** minimally invasive approach compared to surgical aortic valve replacement offers the treatment to a broader patient population,mainly non-surgical candidates.A feared complication of TAVR is the occlusion of coronary artery ostium by the native aortic valve leaflet due to its displacement by the expanded transcatheter *** or native aortic scallop intentional laceration to prevent iatrogenic coronary artery obstruction(BASIliCA)is a technique developed to mitigate this risk by creating a lengthwise laceration of the left and/or right aortic valve leaflets prior to *** outcomes following TAVR with BASIliCA have been *** preoperative examination,patient selection,and hemodynamic management are *** continued refinement,BASIliCA may further expand the application of TAVR to patients at high risk for coronary occlusion associated with the procedure.

关键词： aortic transcatheter continued

Embolic protection device, the next advancement in TAVR?

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Journal of Geriatric Cardiology 2020年第3期17卷 160-163页

作者： david li Nicole Shirakawa Hong liu Department of Anesthesiology and Pain Medicine University of California Davis HealthSacramentoCAUSA

The approval of commercial application transcatheter aortic valve replacement(TAVR)by the FDA in 2011 has revolutionized the management of high-risk surgical patients with severe aortic stenosis(AS).With TAVR being expanded to intermediate surgical risk patients in 2016,the number of patients undergoing TAVR has been increasing *** fact,since the very first human TAVR procedure in 2002,more than 300,000 TAVRs have been performed worldwide.[1,2]The technological advancements in the TAVR since its early days of clinical adaptation has made this procedure a routine practice in many institutions ***,one of the most feared complications,ischemic stroke,remains a major concern for clinicians and patients despite all the advancements in the procedure.

关键词： Embolic protection Stroke Transcatheter aortic valve replacement

Online Power System Voltage Stability Index for LCC HVDC Using Local Measurements

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CSEE Journal of Power and Energy Systems 2023年第1期9卷 100-110页

作者： david li Conghuan Yang Ying Xue Xiao-Ping Zhang Department of Electronic Electrical and Systems EngineeringSchool of EngineeringUniversity of BirminghamBirmingham B152TTUK Guangzhou Maritime University GuangzhouChina School of Electric Power Engineering South China University of TechnologyGuangzhou 510640China

This paper proposes an online power system voltage stability index(PVSI Online)that quantifies power system voltage stability in real time between an AC network and LCC HVDC using only local *** methods relied on using telecommunications to inform a change in a predetermined AC network *** proposed method uses local measurements of voltage and current from the HVDC using a newly developed,numerically robust technique called Moving Window Covariance to detect a change in the AC network in real *** network parameters are fed to a newly devised index that accurately models and captures the dynamics of the interaction between an LCC HVDC and AC *** index is used to quantify stability,but also to advise network *** an onset of instability due to network degradation,the proposed index can provide a power order value for the HVDC to restore *** as a self-contained module that can be implemented within a HVDC scheme.

关键词： LCC HVDC local measurements Moving Window Covariance numerical drift online PVSI PVSI Online real time SCR SCL self-contained

How Did China Maintain Macroeconomic Stability During 1978-2018?

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China & World Economy 2021年第3期29卷 55-82页

作者： Ming Feng david Daokui li Shuyu Wu National Academy of Economic Strategy Chinese Academy of Social SciencesChina Academic Center for Chinese Economic Practice and Thinking(ACCEPT) Tsinghua UniversityChina School of Economics and Resource Management Beijing Normal UniversityChina

In this paper,we analyze the role of macroeconomic management in developing countries'economic take-off and structural *** argue that developing countries face three leading challenges:market immaturity,lack of a developed financial system,and severe information asymmetry between international investors and domestic *** not properly dealt with,these challenges can lead to macroeconomic volatility and fragility in economic ***,the government must intervene appropriately to address these *** analyzing China s experiences in the era of reform and opening up(1978-2018),we find three important lessons:(i)It is important for the government to facilitate the entry and exit of enterprises in macroeconomic cycles,relying not only on market signals but also on administrative orders and measures of institutional reform;(ii)Financial reforms should be implemented in order to promote financial deepening and channel savings into investment;and(iii)The government should carefully manage capital account liberalization in order to preserve financial stability while promoting foreign investment,international trade,and industrial upgrading.

关键词： capital account liberalization China’s macroeconomic management financial deepening macroeconomic stability

Cognitive function assessment should be included in preoperative evaluation

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The Journal of Biomedical Research 2018年第3期32卷 161-163页

作者： david li Hong liu Department of Anesthesiology and Pain Medicine University of California Davis Health

As the aging population continues to increase worldwide,a proportionally increasing number of elderly patients will be undergoing surgical procedures requiring anesthesia *** has been estimated that approximately one ... 详细信息

关键词： anesthesia preoperative amongst elderly older requiring postoperative incidence screening likely

Isolation and identification of novel casein-derived bioactive peptides and potential functions in fermented casein with Lactobacillus helveticus

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Food Science and Human Wellness 2019年第2期8卷 156-176页

作者： Mengzhu Fan Tingting Guo Wanru li Jing Chen Fushuo li Chao Wang Yi Shi david Xi-an li Shaohui Zhang School of Agriculture and Biology Shanghai Jiao Tong UniversityChina Zhejiang Go Peptides life Science and Healthcare Technology Co. Ltd.China School of Systemic Biology and Medicine Shanghai Jiao Tong UniversityChina Zhejiang Panda Dairy Group Co.Ltd. China

The present study here establishes a complete and effective method for isolating,purifying and identifying extracellular and intracellular peptides,and also describes the characters and bioactivities of peptides from fermented casein with Lactobacillus *** peptides are much larger in quantity and more complex in composition than extracellular peptides,between which the correlation reveals proteolytic and metabolic *** addition,totally 241 different peptide sequences were identified by Nano LC–MS/MS from casein(212)and Lactobacillus helveticus proteins(29).These casein-derived peptides mostly originated from-casein,followed byS1-casein,-casein,andS2-casein,and came from extracell(69)and intracell(143),in which common peptides have a total of ***-four of the identified peptides were previously described as bioactive,including angiotensinconverting enzyme(ACE)-inhibitory,antioxidant,immunomodulating,antimicrobial,DPP-IV inhibitory,antiamnesic and anticancer effects and so *** peptides with the potential of some biological activities are obtained,which were described in previous studies.A total of 47 novel peptides of 5 to 26 amino acids that were not disclosed were *** new sources of natural bioactive peptides may have the very high application value as potential new peptide drugs for treatment human *** product peptide DELQDKIHPF found in both extracell and intracell was quantitatively analyzed using the MRM mode of UPLC-U3Q,23.1 and 9.76 ng/mL,*** quantitative analysis of the potential bioactive peptide may also advance the production of peptide products in the future.

关键词： Bioactive peptide Peptide profiling Fermented casein Lactobacillus helveticus

Is a designated arterial catheter indicated in transcatheter aortic valve replacement procedure?

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The Journal of Biomedical Research 2022年第2期36卷 73-76页

作者： Pooja Patel lillian Jundi david li Hong liu Department of Anesthesiology and Pain Medicine University of California Davis HealthSacramentoCA 95817USA California Northstate University College of Medicine SacramentoCA 95757USA

Aortic stenosis(AS)is the most common valvular disorder frequently affecting patients as they get older,and resulting in life-altering symptoms such as dyspnea,angina,pre-syncope,and syncope,which are often made worse with ***,AS is caused by age-related calcification of the valve;in addition,its pathogenetic mechanism also involves the fact that bicuspid aortic valves tend to narrow rapidly compared to other three-leaflet *** the aging population continues to grow,an increasing number of patients are seeking treatment for AS,which makes therapies for AS continuing to evolve and ***,surgical aortic valve replacement(SAVR),requiring a median sternotomy and an extended post-operative intensive care unit stay,was the predominant therapy for patients with AS.

关键词： aortic valve seeking