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Deoxyelephantopin induces ROS-mediated autophagy and apoptosis in human colorectal cancer in vitro and in vivo

Asian Pacific Journal of Tropical Biomedicine 2020年第3期10卷 120-135页

作者： Chim-Kei Chan kind-Leng Tong Pooi-Fong Wong Habsah Abdul Kadir Biomolecular Research Group Biochemistry ProgramInstitute of Biological SciencesFaculty of ScienceUniversity of Malaya50603 Kuala LumpurMalaysia Department of Pharmacology Faculty of MedicineUniversity of Malaya50603 Kuala LumpurMalaysia

Objective:To systematically map the stepwise events leading to deoxyelephantopin-induced cell death of HCT116 human colorectal cancer cells and evaluate the effectiveness of deoxyelephantopin in ***:HCT116 cells were treated with deoxyelephantopin at various concentrations and time *** was confirmed by the detection of autophagosomes and autophagosomal proteins by electron microscopy and Western blotting assays,respectively,and then validated by siRNA *** addition,apoptosis was confirmed by the detection of apoptosis-related *** intracellular reactive oxygen species(ROS)level was measured using flow *** growth inhibitory effect of deoxyelephantopin was further evaluated in vivo using a mouse xenograft ***:Deoxyelephantopin firstly elevated ROS production,which then triggered autophagic flux with the accumulation of autophagosomal proteins including LC3 A/B,ATG5,and ATG7,followed by the induction of apoptosis via the intrinsic and extrinsic ***-treatment with N-acetyl-L-cysteine,a ROS inhibitor,reversed both apoptosis and *** knockdown of LC3 prevented apoptosis induction which confirmed that deoxyelephantopin induced autophagy-dependent apoptosis in HCT116 *** of ROS also activated apoptosis via the mitogen-activated protein kinases signaling ***,deoxyelephantopin also inhibited the PI3 K/AKT/mTOR pathway,which then released the inhibition of *** vivo study further showed that deoxyelephantopin significantly suppressed the growth of HCT116 subcutaneous xenograft in nude ***:Our findings revealed that deoxyelephantopin elevates oxidative stress and induces ROS-dependent autophagy followed by apoptosis in HCT116 cells via the concerted modulation of multiple signaling *** findings further support the development of deoxyelephantopin as a therapeutic agent for colorectal cancer.

关键词： Deoxyelephantopin Autophagy PI3K mTOR Colorectal cancer MAPK

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分离三分量远震接收函数的多道最大或然性反褶积方法

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地震地质 2004年第3期26卷 416-425页

作者：刘启元 Rainer kind 中国地震局地质研究所地震动力学国家重点实验室 GeoForschung Zentrum Potsdam Telegrafenberg14473 PotsdamGermany

迄今为止 ,广泛应用的分离接收函数方法主要基于单个台站的观测数据 ,并依赖等效震源假定的有效性。当地壳内存在明显的间断面时 ,等效震源假定不适于宽频带地震波形数据 ,接收函数的估计将在不同程度上遭到破坏。解决这个问题的出路在... 详细信息

迄今为止 ,广泛应用的分离接收函数方法主要基于单个台站的观测数据 ,并依赖等效震源假定的有效性。当地壳内存在明显的间断面时 ,等效震源假定不适于宽频带地震波形数据 ,接收函数的估计将在不同程度上遭到破坏。解决这个问题的出路在于避免使用等效震源假定。依据多道最大或然性反褶积原理 ,给出了利用多道观测数据分离接收函数的方法。我们的方法避免了等效震源假定 ,这对于在复杂构造环境条件下改善接收函数的估计具有实际价值。合成地震图的数值检验结果验证了给出的方法。利用该方法 ,我们得到了跨越大别山造山带 ,从江西大冶附近的大箕铺 (30°2 0′N ,1 1 5°0 3′E)到河南兰考附近的崔林 (34°4 0′N ,1 1 4°4 9′E) ,总长度约 5 0 0km的流动地震台阵剖面各台站的三分量接收函数估计。与基于单台数据的接收函数径向分量比较表明。

关键词：分离接收函数地震台站地震波形数据震源反褶积

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Neonatal lamb mortality:major risk factors and the potential ameliorative role of melatonin

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Journal of Animal Science and Biotechnology 2021年第2期12卷 451-461页

作者： Tom Flinn david O.Kleemann Alyce M.Swinbourne Jennifer M.Kelly Alice C.Weaver Simon K.Walker Kathryn L.Gatford Karen L.kind William H.E.J.van Wettere Davies Livestock Research Centre School of Animal and Veterinary SciencesThe University of AdelaideRoseworthySAAustralia Turretfield Research Centre South Australian Research and Development InstituteRosedaleSAAustralia Robinson Research Institute Adelaide Medical SchoolThe University of AdelaideAdelaideSAAustralia

High incidences of pre-weaning mortality continue to limit global sheep production,constituting a major economic and welfare *** significant advances in genetics,nutrition,and management,the proportion of lamb deaths has remained stable at 15–20%over the past four *** is mounting evidence that melatonin can improve outcomes in compromised ovine pregnancies via enhanced uterine bloodflow and neonatal *** review provides an overview of the major risk factors and underlying mechanisms involved in perinatal lamb mortality and discusses the potential of melatonin treatment as a remedial *** pregnant ewes with melatonin enhances uterine bloodflow and fetal oxygenation,and potentially birthweight and neonatal thermogenic *** freely crosses the ovine placenta and blood-brain barrier and provides neuroprotection to the fetal lamb during periods of chronic and acute hypoxia throughout gestation,with improved behavioural outcomes in hypoxic *** current literature provides strong evidence that maternal melatonin treatment improves outcomes for lambs which experience compromised in utero development or prolonged parturition,though to date this has not been investigated in livestock production *** such there is a clear basis for continued research into the effects of maternal melatonin supplementation during gestation on pre-weaning survival under extensive production conditions.

关键词： Lamb survival Melatonin Neonatal mortality Reproduction Sheep

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基于芯片的直接进样质谱法和气相色谱-质谱测定莱茵衣藻中的极性脂类

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化学学报 2013年第4期71卷 663-669页

作者：杨大伟范晓蕾 kind Tobias Fiehn Oliver 郭荣波中国科学院青岛生物能源与过程研究所生物燃料重点实验室青岛266101 中国科学院研究生院北京100039 美国加州大学戴维斯分校-基因组中心戴维斯95616

目前微藻的脂类分析主要侧重于脂肪酸酯、甘油三酯以及细胞内总脂的分析,忽视了极性脂类的分析.本研究建立了一个基于芯片的全自动电喷雾进样系统联用线性离子阱质谱的技术,用于分析莱茵衣藻中的极性脂类.通过数据库Lipidblast鉴定出了3... 详细信息

目前微藻的脂类分析主要侧重于脂肪酸酯、甘油三酯以及细胞内总脂的分析,忽视了极性脂类的分析.本研究建立了一个基于芯片的全自动电喷雾进样系统联用线性离子阱质谱的技术,用于分析莱茵衣藻中的极性脂类.通过数据库Lipidblast鉴定出了35种不同种类的极性脂类,并首次鉴定出20种甜菜碱脂类.同时利用了GC/TOF-MS来检测莱茵衣藻中的游离脂肪酸,发现亚油酸、亚麻酸和棕榈酸是莱茵衣藻中含量最丰富的三种脂肪酸.建立的脂类分析方法为微藻脂类种类全面综合的分析提供了方法上的参考.

关键词：极性脂类莱茵衣藻直接进样质谱法气相色谱-质谱游离脂肪酸

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Current Trends in Tall Building Construction

Current Trends in Tall Building Construction

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国际桥梁2004年大会

作者： Steffen kind Prof. Dr.-Ing.University of Applied Sciences Darmstadt Germany Office for Structural Engineering Wiesbaden Germany

＜正＞ Essential tendencies in the international field of tall building construction are shown. The article gives details on the complexity of a variety of requirements originating from esthetics, functionality, economical feasibility and safety, as well as planning, erection, usage （and re-usage） and demolition. On the whole, the tendencies are described as follows: increasing flexibility, filigree full glass facades, integrative planning, improved room climate, modified vibration behaviour, optimised supporting complexes and materials, increased safety requirements.

关键词： tall buildings glass facades room-climate integrative planning vibration structures materials safety.

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Seismic signature of the collision between the east Tibetan escape flow and the Sichuan Basin

Seismic signature of the collision between the east Tibetan ...

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中国科学院地质与地球物理研究所第十届(2010年度)学术年会

作者： Rainer kind Deutsches GeoForschungsZentrum GFZ Telegrafenberg14473 PotsdamGermany Freie Universitat Berlin Germany

GPS displacement vectors show that the crust in east Tibet is being squeezed in an easterly direction by the northward motion of the Indian plate,and the Sichuan Basin is resisting this stream and redirecting it mainly towards *** Longmen Shan,containing the steepest rise to the high plateau anywhere in Tibet, results from the strong interaction between the east Tibetan escape flow and the rigid Yangtze block (Sichuan Basin),but the kinematics and dynamics of this interaction are still the subject of some *** herein present results from a dense passive-source seismic profile from the Sichuan Basin into eastern Tibet in order to study the deep structure of this collision *** P and S receiver function images we observe a sudden rise of the Lithosphere-Asthenosphere Boundary(LAB) from 120 to 150 km beneath the Sichuan Basin and from 70 to 80 km beneath eastern *** contrast,the depth of the crust-mantle boundary (Moho) increases from 36 to 40 km beneath the Sichuan Basin and from 55 to 60 km beneath eastern Tibet. The 410km discontinuity is depressed below eastern Tibet by about 30 km,although the 660 remains at nearly the same depth throughout the *** these observations,we conclude that the mode of collision that occurs between Tibet and the Sichuan Basin is very different to that found between India and *** southern Tibet,we observe in essence the subduction of the Indian plate,which penetrates northwards for several hundred kilometers under central *** very thin mantle part of the lithosphere beneath eastern Tibet may indicate delamination or removal of the bottom of the lithosphere by hot asthenospheric escape *** process leads to the exceptionally steep topography at the eastern Tibetan margin as a result of gravitational *** view is supported by the very unusual depression of the 410 km discontinuity beneath eastern Tibet,which could be caused by the dynamics of the sub-vertical downward asthenospheric f

关键词： east Tibetan escape flow Sichuan Basin receiver function imaging mantle transition zone

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The role of the PI3K/AKT/mTOR pathway in brain tumor metastasis

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Journal of Cancer Metastasis and Treatment 2016年第1期2卷 80-89页

作者： Silvia Crespo Marcus kind Alexandre Arcaro Division of Pediatric Hematology/Oncology Bern University HospitalCH-3008 BernSwitzerland

The PI3K/AKT/mTOR(PAM)pathway is involved in a variety of cellular functions and often contributes to oncogenesis and cancer *** has been recognized that this pathway is frequently activated in the most common central nervous system cancers of adults and children,malignant gliomas and medulloblastomas(MB).In these tumors,the PAM network controls key functions necessary for cell invasion and metastasis,such as cell *** review summarizes the current knowledge about the role of PAM signaling in cell invasion and metastasis in gliomas and *** approaches to inhibit cell invasion and metastasis by targeting the PAM pathway will also be discussed.

关键词： PI3K/AKT/mTOR pathway glioblastoma medulloblastoma metastasis

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A Generic Polycarbonate Based Microfluidic Tool to Study Crystal Nucleation in Microdroplets

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Journal of Crystallization Process and Technology 2018年第1期8卷 1-17页

作者： Daniel Selzer Burkard Spiegel Matthias kind Institute of Thermal Process Engineering Karlsruhe Institute of Technology (KIT) Karlsruhe Germany

Crystal nucleation is important to control the product properties in industrial crystallization processes. To investigate crystallization phenomena, methods which rely on microscopic volumes have gained relevance over the last decade. Microfluidic devices are suitable for carrying out crystallization experiments based on a large set of individual droplets in the nanoliter range. In this work, we propose a simple method to manufacture such devices from polycarbonate as an alternative to conventional chips made of poly (dimethylsiloxane). The microfluidic device consists of two main functional parts: A T-junction for droplet generation and a section for storage and observation of up to 400 individual droplets. Using these manufactured devices, it is easy to produce and store highly monodisperse droplets of substances that require either a hydrophilic or hydrophobic surface of the microchannel. Since crystal nucleation is a stochastic process which depends on the sample volume, a reproducible droplet volume is of great importance for crystallization experiments. The versatile applicability of the manufactured devices is demonstrated for substances which are used in different crystallization applications, for example, solution crystallization (aqueous potassium nitrate solution) and melt crystallization (ethylene glycol distearate). Finally, we demonstrate that the manufactured microfluidic devices in our experimental setup can be used to conduct crystal nucleation measurements. Based on these measurements we discuss our results with respect to state-of-the-art nucleation models.

关键词： Microfluidics Polycarbonate Crystallization Electrolyte Solution Organic Melt

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Review of the impact of heat stress on reproductive performance of sheep

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Journal of Animal Science and Biotechnology 2021年第3期12卷 859-876页

作者： William H.E.J.van Wettere Karen L.kind Kathryn L.Gatford Alyce M.Swinbourne Stephan T.Leu Peter T.Hayman Jennifer M.Kelly Alice C.Weaver david O.Kleemann Simon K.Walker The University of Adelaide School of Animal and Veterinary SciencesDavies Livestock Research CentreRoseworthy CampusMudla Wirra RdRoseworthySouth Australia 5371Australia The University of Adelaide Robinson Research InstituteAdelaide Medical SchoolNorth TerraceAdelaideSouth Australia 5000Australia The University of Adelaide School of AgricultureFood and WineWaite Research InstituteUrrbraeSouth Australia 5064Australia South Australian Research and Development Institute Primary Industries and Regions SAGovernment of South AustraliaClimate ApplicationsWaite Research PrecinctUrrbraeSouth Australia 5064Australia South Australian Research and Development Institute Primary Industries and Regions SAReproductive BiologyLivestock SciencesTurretfield Research Centre129 Holland RdRosedaleSouth Australia 5350Australia

Heat stress significantly impairs reproduction of sheep,and under current climatic conditions is a significant risk to the efficiency of the meat and wool production,with the impact increasing as global temperatures *** from field studies and studies conducted using environmental chambers demonstrate the effects of hot temperatures(≥32℃)on components of ewe fertility(oestrus,fertilisation,embryo survival and lambing)are most destructive when experienced from 5 d before until 5 d after *** controlled studies also demonstrate that ram fertility,as measured by rates of fertilisation and embryo survival,is reduced when mating occurs during the period 14 to 50 d ***,the contribution of the ram to heat induced reductions in flock fertility is difficult to determine *** primarily on temperature controlled studies,it is clear that sustained exposure to high temperatures(≥32℃)during pregnancy reduces lamb birthweight and will,therefore,decrease lamb survival under field *** is concluded that both ewe and ram reproduction is affected by relatively modest levels of heat stress(≥32℃)and this is a concern given that a significant proportion of the global sheep population experiences heat stress of this magnitude around mating and during *** this,strategies to limit the impacts of the climate on the homeothermy,behaviour,resource use and reproduction of extensively grazed sheep are limited,and there is an urgency to improve knowledge and to develop husbandry practices to limit these impacts.

关键词： Fertility Heat stress Oogenesis Pregnancy Sheep Spermatogenesis

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硼酸盐梯度折射率玻璃

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硅酸盐学报 1994年第5期22卷 427-433页

作者：孙雨南 kind.,DS 北京理工大学工程光学系

研制了几种由离子交换形成梯度折射率的铝－硼酸盐玻璃。通过Ｌｉ￣（＋）和Ｎａ￣（＋）离子交换分别获得了了负轴向折射率梯度。折射率差达０．０３，梯度Ａｂｂｅ数大于１００。

关键词：离子交换梯度折射率硼酸盐玻璃

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