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ARMILLARIA TRAPPING IN A PINUS KORAIENSIS PLANTATION IN NORTHEAST cHINA

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Journal of Northeast Forestry University 1992年第2期3卷 1-12页

作者： david waigcck ip Forestry canada Winnipeg Manitoba Canada R3C 1A6

Trap bags were used to assess the presence of an Armillaria species in a Pinus koraiensis plantation in Heilongjiang, china. 216 trap bags containing bark were placed in three plots at 2×2m spacing. After 10 weeks, the presence of Armillaria was determined from the growth of rhizomorphs in the trap bags. The trap bag method proved successful in determining the presence of viable Armillaria rhizomorphs where only a few dead trees showed signs of the disease. The distribution of positive traps corresponded generally with the presence of rhizomorphs in the soil taken from the trap holes. However, the trap bags were superior to soil samples for evaluating the presence of viable rhizomorphs. The fresh, abundant rhizomorphs were easily identified by persons inexperienced in the assessment of Armillaria root rot. Several practical problems need to be solved before the method is applicable in general forest management in china.

关键词： Armillaria,Disease management Northeast china Pinus koraiensis Plantation discase Trapping

Silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

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Neural Regeneration Research 2025年第6期20卷 1789-1800页

作者： carina Hromada Patrick Heimel Markus Kerbl LászlóGál Sylvia Nürnberger Barbara Schaedl James Ferguson Nicole Swiadek Xavier Monforte Johannes c.Heinzel Antal Nógrádi Andreas H.Teuschl-woller david Hercher Department Life Science Engineering University of Applied Sciences Technikum WienViennaAustria Austrian cluster for Tissue Regeneration ViennaAustria Ludwig Boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University clinic of Dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medical University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of Hand- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tNGcs). However, the use of tNGcs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGc would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tNGcs and compared the various features of these improved silk^(+) tNGcs with the tubes without holes(silk^(–) tNGcs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tNGcs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tNGcs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tNGcs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tNGcs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimizatio

关键词： axon regeneration blood vessel functional recovery macroporous nerve lesion peripheral nerve repair sciatic nerve silk-based nerve guidance conduit vascularization

VPOT:A customizable Variant Prioritization Ordering Tool for Annotated Variants

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Genomics, Proteomics & Bioinformatics 2019年第5期17卷 540-545页

作者： Eddie ip Gavin chapman david winlaw Sally LDunwoodie Eleni Giannoulatou Victor chang cardiac Research Institute Sydney 2010Australia St Vincent’s clinical School University of New South WalesSydney 2052Australia Heart centre for children The Children’s Hospital at WestmeadSydney 2145Australia Sydney Medical School University of SydneySydney 2050Australia School of Biotechnology and Biomolecular Sciences University of New South WalesSydney 2033Australia

Next-generation sequencing(NGS) technologies generate thousands to millions of genetic variants per *** of potential disease-causal variants is labor intensive as it relies on filtering using various annotation metrics and consideration of multiple pathogenicity prediction *** have developed VPOT(variant prioritization ordering tool),a python-based command line tool that allows researchers to create a single fully customizable pathogenicity ranking score from any number of annotation values,each with a user-defined *** use of VPOT can be informative when analyzing entire cohorts,as variants in a cohort can be *** also provides additional functions to allow variant filtering based on a candidate gene list or by affected status in a family *** outperforms similar tools in terms of efficacy,flexibility,scalability,and computational *** is freely available for public use at Git Hub(https://***/VccRI/VPOT/).Documentation for installation along with a user tutorial,a default parameter file,and test data are provided.

关键词： Next-generation sequencing Pathogenicity predictions Variant prioritization customizable ranking Genomic annotation

More than mimicry？ Evaluating scope for flicker-fusion as a defensive strategy in coral snake mimics

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current Zoology 2014年第1期60卷 123-130页

作者： Georgia c. TITcOMB david w. KIKUcHI david w. PFENNIG Department of Biology University of North Carolina at Chapel Hill Chapel Hill NC 27599-3280

coral snakes and their mimics often have brightly colored banded patterns, generally associated with warning colora- tion or mimicry. However, such color patterns have also been hypothesized to aid snakes in escaping predators through a ＂flicker-fusion＂ effect. According to this hypothesis, banded color patterns confuse potential predators when a snake transitions from resting to moving because its bands blur together to form a different color. To produce this motion blur, a moving snake＇s bands must transition faster than the critical flicker-fusion rate at which a predator＇s photoreceptors can refresh. It is unknown if coral snakes or their mimics meet this requirement. we tested this hypothesis by measuring the movement speed and color pat- terns of two coral snake mimics, Lampropeltis triangulum campbelli and L. elapsoides, and comparing the frequency of color transitions to the photoreceptor activity of the avian eye. we found that snakes often produced a motion blur, but moving snakes created a blurring effect more often in darker conditions, such as sunrise, sunset, and nighttime when these snakes are often active. Thus, at least two species of coral snake mimics are capable of achieving flicker-fusion, indicating that their color patterns may confer an additional defense aside from mimicry

关键词： coral snake mimicry Flicker-fusion effect cFF Kingsnake Defensive strategy

Longwall automation: Delivering enabling technology to achieve safer and more productive underground mining

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International Journal of Mining Science and Technology 2015年第6期25卷 865-876页

作者： Ralston Jonathon c. Reid david c. Dunn Mark T. Hainsworth david w. coal Mining Research Program CSIRO Energy

The ongoing need to deliver improved safety, productivity and environmental benefit in coal mining presents an open challenge as well as a powerful incentive to develop new and improved solutions. This paper assesses the critical role that enabling technologies have played in the delivery of remote and automated capability for longwall mining. A brief historical account is given to highlight key technical contributions which have influenced the direction and development of present-day longwall technology. The current state of longwall automation is discussed with particular attention drawn to the technologies that enable automated capability. Outcomes are presented from an independently conducted case study that assessed the impact that cSIRO＇s LASc longwall automation research has made to the longwall mining industry in Australia. Importantly, this study reveals how uptake of this innova- tive technology has significantly benefitted coal mine productivity, improved working conditions for personnel and enhanced environmental outcomes. These benefits have been widely adopted with cSIRO automation technology being used in 60 per cent of all Australian underground operations. International deployment of the technology is also emerging. The paper concludes with future challenges and opportunities to highfight the ongoing scope for longwall automation research and development.

关键词： Safety Productivity Environment Mining automation Longwall mining Remote mining

Use of non-timber forest products from invasive alien Prosopis species(mesquite) and native trees in South Africa: implications for management

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Forest Ecosystems 2015年第3期2卷 197-207页

作者： Ross T Shackleton david c Le Maitre Brian w van wilgen david M Richardson centre for Invasion Biology Department of Botany and ZoologyStellenbosch University centre for Invasion Biology Natural Resources and the EnvironmentCSIR

Background: Prosopis species have been introduced to many areas outside their native range to provide benefits to local communities. Several Prosopis species and their hybrids(hereafter "mesquite") have, however, become naturalised and invasive and now generate substantial costs. Management options are limited because of the complex conflicts of interest regarding benefits and costs. Management policies and strategies must take account of such conflicts, but further insights are needed on the dimensions of uses and impacts before such information can be usefully applied. current policy in South Africa allows for the growth and use of mesquite in one province,but not in others where its control is mandatory. we report on a study to quantify the direct use and perceptions of non-timber forest products(NTFPs) from mesquite and native trees in South ***: Semi-structures household interviews were conducted with various stakeholder groups to identify what tree products are used, to ascertain amounts used as well as to gauge perceptions of natural resource use between different tree species and use over ***: The direct household use value of native trees was higher than that of mesquite, and local stakeholders attached greater value to products from native trees than from mesquite. Therefore, native trees are and will still be preferentially harvested, and mesquite is unlikely to offer protection to native species by providing an alternative source of products. Mesquite pods do, however, provide valuable additional resources(fodder and medicinal products). The use of both native trees and mesquite is decreasing as the incomes of poorer households rise and as alternative energy sources become available. The benefits and reliance on mesquite are not as high as previously assumed and the impacts from mesquite invasions create large problems for local ***: This study provides further evidence that the impacts of mesquite exceed the benefi

关键词： Biological invasions conflicts of interests cost v

Quantifying local fire regimes using the Landsat data-archive:a conceptual framework to derive detailed fire pattern metrics from pixel-level information

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International Journal of Digital Earth 2019年第5期12卷 544-565页

作者： Ignacio San-Miguel david w.Andison Nicholas c.coops Integrated Remote Sensing Studio Department of Forest Resources ManagementUniversity of British ColumbiaVancouverBCCanada Bandaloop Landscape-Ecosystem Services North VancouverBCCanada

Free and open access to the Landsat archive has enabled the detection and delineation of an unprecedented number of fire events across the *** the availability and potential of these data,few studies have analysed residual vegetation patterns and/or partial mortality of fire across the canadian boreal forest,and those available,are either incomplete or ***,they all differ in the methods and spatial language,which makes it difficult for managers to interpret fire patterns over large *** is an urgent need for methods to help unify fire pattern observations across the canadian boreal *** study explores the capacity of the Landsat data archive when coupled with a recently developed fire mapping approach and a robust spatial language to characterize and compare tree mortality patterns across the boreal plains ecozone,*** 507 fires 2.5 Mha mapped,this study represents the most comprehensive analysis of mortality patterns for study *** from this demonstration generated an accurate characterization of the fire patterns the various ecoregions based on seven key fire *** comparison between ecoregions revealed differences in the amount of residual vegetation,which in turn suggested various climate,topography and/or vegetation ecosystem drivers.

关键词： Fire emulation historical range of variability landscape metrics fire patterns disturbance-based management

Recombinase-mediated Gene Stacking as a Transformation Operating System

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Journal of Integrative Plant Biology 2011年第7期53卷 512-519页

作者： david w.Ow South china Botanical Garden

The current method for combining transgenes into a genome is through the assortment of independent loci, a classical operating system compatible with transgenic traits created by different developers, at different times and/or through different transformation techniques. However, as the number of transgenic loci increases over time, increasingly larger populations are needed to find the rare individual with the desired assortment of transgenic loci along with the non-transgenic elite traits. Introducing a transgene directly into a field cultivar would bypass the need to introgress the engineered trait. However, this necessitates separate transformations into numerous field cultivars, along with the characterization and regulatory approval of each independent transformation event. Reducing the number of segregating transgenic loci could be achieved if multiple traits are introduced at the same time, a preferred option if each of the many traits is new or requires re-engineering. If reengineering of previously introduced traits is not needed, then appending a new trait to an existing locus would be a rational strategy. The insertion of new DNA at a known locus can be accomplished by site- specific integration, through a host-dependent homology-based process, or a heterologous site-specific recombination system. Here, we discuss gene stacking through the use of site-specific recombinases.

关键词： site-specific recombination transgenic plants DNA integration marker removal cre-lox

A Review of the Taxonomy and Palaeoecology of the Anurognathidae(Reptilia,Pterosauria)

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Acta Geologica Sinica(English Edition) 2020年第5期94卷 1676-1692页

作者： david w.E.HONE School of Biological and chemical Sciences Queen Mary University of LondonLondonE14NSUK

The anurognathids are an enigmatic and distinctive clade of small,non-pterodactyloid pterosaurs with an unusual combination of anatomical traits in the head,neck,wings and *** are known from very limited remains and few have been described in detail,and as a result,much of their biology remains *** is despite their importance as potentially one of the earliest branches of pterosaur evolution or even lying close to the origins of *** review covers the taxonomy and palaeoecology of the anurognathids,which remain an interesting branch of pterosaurian evolution.

关键词： non-pterodactyloid palaeobiology flight Ornithodira