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A systematic scoping review of study methodology for randomized controlled trials investigating probiotics in athletic and physically active populations

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Journal of Sport and Health Science 2024年第1期13卷 61-71页

作者： Alex E.Mohr david b.pyne.ge.vana sil.a fogaça l.ite.de.orah akins Jamie Pugh College of Health Solutions Arizona State UniversityPhoenixAZ 85004USA Research Institute for Sport and Exercise University of CanberraCanberraACT 2617Australia laboratory of Functional Fermented Food Faculty of Pharmaceutical SciencesUniversity of São PauloSão Paulo 05508-030Brazil Research Institute for Sport and Exercise Sciences Liverpool John Moores UniversityLiverpool L33AFUK

background:The purported ergogenic and health effects of probiotics have been a topic of great intrigue among researchers,practitioners,and the lay public *** has also been an increased research focus within the realm of sports science and exercise medicine on the athletic gut ***,compared to other ergogenic aids and dietary supplements,probiotics present unique study *** objectives of this systematic scoping review were to identify and characterize study methodologies of randomized controlled trials investigating supplementation with probiotics in athletes and physically active ***:Four databases(MEDlINE,CINAHl,Cochrane CENTRAl,and Cochrane Database of Systematic Reviews)were searched for randomized controlled studies involving healthy athletes or physically active *** intervention with probiotics and inclusion of a control and/or placebo group were *** peer-reviewed articles in English were considered,and there were no date *** were extracted and presented in tabular form to detail study protocols,characteristics,and *** in randomized controlled trials was determined with the Rob 2.0 ***:A total of 45 studies were included in the review,with 35 using a parallel group design and 10 using a cross-over *** half the studies used a single probiotic and the other half a multi-strain *** probiotic dose ranged from 2×10^(8)to 1×10^(11)colony forming units daily,and the length of intervention was between 7 and 150 *** than half the studies directly assessed gastrointestinal symptoms,gut permeability,or the gut *** sex ratio of participants was heavily weighted toward males,and only 3 studies exclusively investigated ***-level adverse events were reported in only 2 studies,although the methodology of reporting varied *** risk of bias was generally low,although details on randomization were lacking in some s

关键词： Exercise Experimental methodology Gastrointestinal symptoms Gut microbiota Probiotic supplementation

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genome-wide SNP genotyping Resolves Signatures of Selection and Tetrasomic Recombination in Peanut

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Molecular Plant 2017年第2期10卷 309-322页

作者： Josh Clevenger Ye Chu Carolina Chavarro Gaurav Agarwal david J. bertioli Soraya C.M. leaI-bertioli Manish K. Pandey Justin Vaughn brian Abernathy Noelle A. barkley Ran Hovav Mark burow Spurthi N. Nayak Annapurna Chitikineni Thomas G. Isleib C. Corley Holbrook Scott A. Jackson Rajeev K. Varshney Peggy Ozias-akins department of Horticulture and Institute of Plant breeding Genetics & Genomics The University of Georgia 2356 Rainwater Road Tifton GA 31793 USA Center for Applied genetic Technologies and Institute of Plant breeding Genetics & Genomics The University of Georgia Athens GA 30602 USA International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Hyderabad 502324 India University of brasil.a Institute of Biological Sciences Campus Darcy Ribeiro 70910-900 Brasilia DF Brazil Embrapa genetic Resources and biotechnology 70770-917 Brasflia DF Brazil eUSDA-ARS PGRCU Griffin GA 30223 USA Agricultural Research Organization Plant Sciences Institute 7528809 Rishon LeZion Israel department of Plant and Soil Science Texas Tech University Lubbock TX 79409-2122 USA department of Crop and Soil Sciences North Carolina State University Box 7629 Raleigh NC 28695-7629 USA USDA-ARS Tifton GA 31793. USA

Peanut （Arachis hypogaea; 2n = 4x = 40） is a nutritious food and a good source of vitamins, minerals, and healthy fats. Expansion of genetic and genomic resources for genetic enhancement of cultivated peanut has gained momentum from the sequenced genomes of the diploid ancestors of cultivated peanut. To facil- itate high-throughput genotyping of Arachis species, 20 genotypes were re-sequenced and genome-wide single nucleotide poiymorphisms （SNPs） were selected to develop a large-scale SNP genotyping array. For flexibility in genotyping applications, SNPs polymorphic between tetraploid and diploid species were included for use in cultivated and interspecific populations. A set of 384 accessions was used to test the array resulting in 54 564 markers that produced high-quality polymorphic clusters between diploid species, 47 116 polymorphic markers between cultivated and interspecific hybrids, and 15 897 polymorphic markers within A. hypogaea germplasm. An additional 1193 markers were identified that illuminated genomic re- gions exhibiting tetrasomic recombination. Furthermore, a set of elite cultivars that make up the pedigree of US runner germplasm were genotyped and used to identify genomic regions that have undergone pos- itive selection. These observations provide key insights on the inclusion of new genetic diversity in culti- vated peanut and will inform the development of high-resolution mapping populations. Due to its efficiency, scope, and flexibility, the newly developed SNP array will be very useful for further genetic and breeding applications in Arachis.

关键词： single nucleotide polymorphism groundnut Arachis hypogaea

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