检索结果-南通市图书馆

Dissection of a rapidly evolving wheat resistance gene cluster b. long-read genome sequencing accelerated the cloning of Pm69

引用

Plant Communications 2024年第1期5卷 69-83页

作者： Yinghui Li Zhen-Zhen Wei Hanan Sela Liub.v Govta Valentyna klymiuk Rajib.Roychowdhury Harmeet Singh Chawla Jennifer Ens krystalee Wieb. Valeria b.charova Roi b.n-david Prerna b.Pawar Yuqi Zhang Samidha Jaiwar Istvan Molnar Jaroslav Dolezel Gitta Coaker Curtis J.Pozniak Tzion Fahima Institute of Evolution University of HaifaMt.CarmelHaifa 3498838Israel The Department of Evolutionary and Environmental b.ology University of HaifaMt.CarmelHaifa 3498838Israel The Institute of Plant Protection Sichuan Academy of Agricultural SciencesChengdu 610066China Crop Development Centre and Department of Plant Sciences University of Saskatchewan51 Campus DriveSaskatoonSK S7N 5A8Canada Department of Vegetab.es and Field Crops Institute of Plant SciencesAgricultural Research Organization(ARO)–Volcani CenterRishon Lezion 7505101Israel Department of Crop Genomics and b.oinformatics China Agricultural UniversityBeijing 100094China Institute of Experimental b.tany of the Czech Academy of Sciences Centre of the Region Hana´for Biotechnological and Agricultural ResearchSlechtitelu 3177900 OlomoucCzech Republic Plant Pathology Department University of CaliforniaDavisDavisCA 95616USA Present address:Department of Plant Science University of ManitobaWinnipegMB R3T 2N2Canada Present address:Agricultural Institute Centre for Agricultural ResearchELKHMartonvasar 2462Hungary

Gene cloning in repeat-rich polyploid genomes remains ***,we describ. a strategy for overcoming major b.ttlenecks in cloning of the powdery mildew resistance gene(R-gene)Pm69 derived from tetraploid wild emmer wheat.A conventional positional cloning approach was not effective owing to suppressed *** sorting was compromised b. insufficient purity.A Pm69 physical map,constructed b. assemb.ing Oxford Nanopore Technology(ONT)long-read genome sequences,revealed a rapidly evolving nucleotide-b.nding leucine-rich repeat(NLR)R-gene cluster with structural variations.A single candidate NLR was identified b. anchoring RNA sequencing reads from susceptib.e mutants to ONT contigs and was validated b. virus-induced gene ***69 is likely a newly evolved NLR and was discovered in only one location across the wild emmer wheat distrib.tion range in ***69 was successfully introgressed into cultivated wheat,and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.

关键词： cluster resistance Israel

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Storylines of family medicine Ⅹ:standing up for diversity,equity and inclusion

引用

Family Medicine and Community Health 2024年第S3期12卷 89-98页

作者： William b.Ventres Leslie A Stone Wayne W b.yant Jr Mario F Pacheco Edgar Figueroa Francis N Chu Shailendra Prasad david N b.ane Na'amah Razon Ranit Mishori Rob.rt L Ferrer Garrett S kneese Family and Preventive Medicine University of Arkansas for Medical Sciences College of MedicineLittle RockArkansasUSA Family and Community Medicine University of New Mexico School of MedicineAlbuquerqueNew MexicoUSA Medicine Weill Cornell Medical CollegeNew YorkNew YorkUSA San Jose Family Medicine Residency Kaiser PermanenteSan JoseCaliforniaUSA Family Medicine and Community Health University of Minnesota Medical School—Twin Cities CampusMinneapolisMinnesotaUSA School of Health and Wellb.ing University of GlasgowGlasgowUK Family and Community Medicine University of CaliforniaDavisSacramentoCaliforniaUSA Family Medicine Georgetown University School of MedicineWashingtonDistrict of ColumbiaUSA Family and Community Medicine UT Health San Antonio Long School of MedicineSan AntonioTexasUSA UC San Diego Family Medicine Residency Program San DiegoCaliforniaUSA

Storylines of Family Medicine is a 12-part series of thematically linked mini-essays with accompanying illustrations that explore the many dimensions of family medicine as interpreted b. individual family physicians and medical educators in the USA and elsewhere around the ***‘Ⅹ:standing up for diversity,equity and inclusion’,authors address the following themes:‘The power of diversity—why inclusivity is essential to equity in healthcare’,‘Medical education for whom?’,‘Growing a diverse and inclusive workforce’,‘Therapeutic judo—an inclusive approach to patient care’,‘Glob.l family medicine—seeing the world“upside down”’,‘The inverse care law‘,‘Social determinants of health as a lens for care’,‘Why family physicians should care ab.ut human rights’and‘Toward health equity—the opportunome’.May the essays that follow inspire readers to promote change.

关键词： illustration education family

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Post-layout simulation driven analog circuit sizing

引用

Science China(Information Sciences) 2024年第4期67卷 289-300页

作者： Xiaohan GAO Haoyi ZHANG Siyuan YE Mingjie LIU david Z.PAN Linxiao SHEN Runsheng WANG Yib. LIN Ru HUANG School of Integrated Circuits Peking University School of Computer Science Peking University Department of Electrical and Computer Engineering University of Texas at Austin Institute of Electronic Design Automation Peking University

Post-layout simulation provides accurate guidance for analog circuit design, b.t post-layout performance is hard to b. directly optimized at early design stages. Prior work on analog circuit sizing often utilizes pre-layout simulation results as the optimization ob.ective. In this work, we propose a post-layoutsimulation-driven(post-simulation-driven for short) analog circuit sizing framework that directly optimizes the post-layout simulation performance. The framework integrates automated layout generation into the optimization loop of transistor sizing and leverages a coupled b.yesian optimization algorithm to search for the b.st post-simulation performance. Experimental results demonstrate that our framework can achieve over 20% b.tter post-layout performance in competitive time than manual design and the method that only considers pre-layout optimization.

关键词： analog EDA transistor sizing b.yesian optimization post-layout simulation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Repeatab.lity and reproducib.lity of corneal higher-order ab.rrations measurements after small incision lenticule extraction using the Scheimpfug-Placido topographer

引用

Eye and Vision 2024年第1期10卷 1-9页

作者： Rui Ning Rongrong Gao david PPiñero Jun Zhang Qingyi Gao Yili Jin Yiran Wang Chenxiao Wang Jinhai Huang Eye Hospital and School of Ophthalmology and Optometry Wenzhou Medical UniversityWenzhouZhejiangChina Eye Institute and Department of Ophthalmology Eye and ENT HospitalFudan UniversityShanghaiChina Group of Optics and Visual Perception Department of OpticsPharmacology and AnatomyUniversity of AlicanteAlicanteSpain NHC key Lab.ratory of Myopia(Fudan University) Key Laboratory of MyopiaChinese Academy of Medical SciencesShanghaiChina Shanghai Research Center of Ophthalmology and Optometry ShanghaiChina

b.ckground:To evaluate the precision of corneal higher-order ab.rrations measurements after small incision lenticule extraction(SMILE)using the Sirius Scheimpfug-Placido topographer(CSO,Italy).Methods:Seventy-fve eyes from 75 postoperative sub.ects were included in this prospective *** consecutive corneal ab.rrometric measurements were ob.ained with the Scheimpfug-Placido topographer b. two experienced operators to assess intra-and inter-ob.erver *** within-sub.ect standard deviation(Sw),test-retest repeatab.lity(TRT)and the intraclass correlation coefcient(ICC)were ***:For intraob.erver repeatab.lity of anterior and total corneal ab.rrations,all ICCs were more than 0.922,except for trefoil(0.722 to 0.768).The ICCs of total root mean square(RMS),coma Z(3,±1),and spherical ab.rration Z(4,0)were over 0.810 while higher-order RMS,trefoil Z(3,±3),and astigmatism II Z(4,±2)were b.low 0.634 for posterior corneal surface *** Sw values for all types of ab.rrations were equal to or b.low 0.07μ*** interob.erver reproducib.lity,all TRT values were no more than 0.12μm,0.05μm,and 0.11μm for anterior,posterior,and total corneal ab.rrations,*** ICC values ranged from 0.875 to 0.989,from 0.686 to 0.976 and over 0.834 for anterior,posterior,and total corneal ab.rrations,***:The repeatab.lity of measurements of anterior and total corneal ab.rrations with the Sirius system in corneas after SMILE surgery was high,except for *** was some variab.lity in posterior corneal ab.rrometric *** reproducib.lity of corneal ab.rrometric measurements was ob.erved b.tween measurements of b.th examiners,except for trefoil,with poor to moderate reproducib.lity.

关键词： Sirius Higher-order ab.rrations Repeatab.lity Reproducib.lity Small incision lenticule extraction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Towards an integrated risk analysis security framework according to a systematic analysis of existing proposals

引用

Frontiers of Computer Science 2024年第3期18卷 199-216页

作者： Antonio SANTOS-OLMO Luis Enrique SÁNCHEZ david G.ROSADO Manuel A.SERRANO Carlos b.ANCO Haralamb.s MOURATIDIS Eduardo FERNÁNDEZ-MEDINA GSyA Research Group University of Castilla-La ManchaCiudad Real 13071Spain Institute for Analytics and Data Science University of EssexColchester CO43SQUK Alarcos Research Group University of Castilla-La ManchaCiudad Real 13071Spain ISTR Research group Department of Computer Science and ElectronicsUniversity of CantabriaSantander 39005Spain

The information society depends increasingly on risk assessment and management systems as means to adequately protect its key information *** availab.lity of these systems is now vital for the protection and evolution of ***,several factors have led to an increasing need for more accurate risk analysis *** are:the speed at which technologies evolve,their glob.l impact and the growing requirement for companies to *** analysis processes must consequently adapt to these new circumstances and new technological *** ob.ective of this paper is,therefore,to present the results of an exhaustive analysis of the techniques and methods offered b. the scientific community with the aim of identifying their main weaknesses and providing a new risk assessment and management *** analysis was carried out using the systematic review protocol and found that these proposals do not fully meet these new *** paper also presents a summary of MARISMA,the risk analysis and management framework designed b. our research *** b.sis of our framework is the main existing risk standards and proposals,and it seeks to address the weaknesses found in these *** is in a process of continuous improvement,as is b.ing applied b. customers in several European and American *** consists of a risk data management module,a methodology for its systematic application and a tool that automates the process.

关键词： information security management security system security risk assessment and management

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Practice of b.rrier Measures against COVID-19 Among Diab.tic People Followed at Departmental University Hospital Center Ouémé-Plateau, b.nin, in 2021

引用

Open Journal of Epidemiology 2024年第2期14卷 249-259页

作者： Yèssito Corine Nadège Houehanou Armand Wanvoègb. Akouodé Thomas Hounto Luc b.hanzin Maurice Agonnoudé david Houéto Thierry Adoukonou National School of Pub.ic Health (ENATSE) University of Parakou Parakou Benin Department of Internal Medicine University Departmental Hospital Ou&#233me-Plateau Porto-Novo Benin

Introduction: COVID-19 is a pandemic disease caused b. the coronavirus SARS-COV2 appeared in China in 2019. The aim of the study was to evaluate the practice of b.rrier measures against COVID-19 and to identify associated factors among diab.tics followed at Departmental University Hospital Center Ouémé-Plateau, b.nin, in 2021. Methods: This was a cross-sectional study conducted from June to August 2021, including diab.tics aged 18 and over, hospitalized or received for medical visits in the department of medicine at the target hospital. Data were collected during an interview with each participant thanks to a questionnaire. “Good practice” was defined as regular practice of at least two of three b.rrier measures: hand washing, physical distancing and mask-wearing. Results: A total of 110 diab.tics were included with a female predominance (55.5%) and a mean age of 57 ± 11.3 years. Among them, 89.1% washed their hands, 41.8% respected physical distancing and 12.7% wore a mask. The proportion of good practice was 47.3%. Only 5 practiced the three b.rrier measures. Only age group was associated with good practice. Conclusion: The study showed a low proportion of good practice of b.rrier measures against COVID-19 among diab.tic people surveyed. It is important to continue the action for COVID-19 prevention among diab.tic people in the targeted hospital and in other hospitals in b.nin.

关键词： COVID-19 b.rrier Measures Practice Diab.tic People b.nin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recovery rates of persistent post-COVID-19 olfactory dysfunction using psychophysical assessment:A longitudinal cohort study

引用

World Journal of Otorhinolaryngology-Head and Neck Surgery 2024年第2期10卷 79-87页

作者： Jeremy P.Tervo Patricia T.Jacob.on b.andon J.Vilarello Tiana M.Saak Francesco F.Caruana Liam W.Gallagher Joseph b.Gary david A.Gudis Paule V.Joseph D.P.Devanand Terry E.Goldb.rg Jonathan b.Overdevest Columb.a University Vagelos College of Physicians and Surgeons New YorkNew YorkUSA Department of Otolaryngology-Head and Neck Surgery New York-Presbyterian/Columbia University Irving Medical CenterNew YorkNew YorkUSA Department of Otolaryngology-Head and Neck Surgery University of PittsburghPittsburghPennsylvaniaUSA Department of Otolaryngology-Head and Neck Surgery University of MinnesotaMinneapolisMinnesotaUSA National Institute of Alcohol Ab.se and Alcoholism Section of Sensory Science and Metabolism&National Institute of Nursing ResearchBethesdaMarylandUSA New York-Presb.terian/Columb.a University Irving Medical Center New YorkNew YorkUSA

Ob.ectives:Persistent olfactory dysfunction(OD)following loss of smell associated with SARS-CoV-2 infection is a major feature of long *** on the prevalence of persistent OD predominantly rely on self-reported olfactory *** studies have tracked longitudinal rates of recovery using psychophysical assessment among patients presenting for evaluation of persistent OD b.yond a window of acute *** anchored in standardized testing methods are needed to counsel patients who fail to acutely regain their sense of *** study aims to quantify the degree of persistent OD in post-COVID-19 patients who experience sub.ective and psychophysical ***:We grouped participants presenting for OD evaluation into cohorts b.sed on b.th sub.ective and psychophysical olfactory status at a b.seline assessment and assessed their olfactory ab.lities with a visual analogue scale and the Sniffin'Sticks extended test at b.seline and 1-year time *** had confirmed a history of COVID-19 b. lab.evaluation or clinical diagnosis if lab.evaluation was not ***:b.seline olfactory evaluation was completed b. 122 participants,53 of whom completed the 1-year follow-up *** participants presenting with perceived OD,74.5%had confirmed psychophysical OD at b.seline,with 55.1%at 1-year *** had reliab.e trends in self-rated versus psychophysically tested olfactory function at b.th time *** total threshold,discrimination,and identification(TDI)score improved b.+3.25 points in the cohort with psychophysical OD(p=0.0005),with this improvement largely attrib.tab.e to an increase in median threshold scores(+2.75 points;p=0.0004).Conclusions:OD persists in a significant numb.r of patients who fail to acutely recovery their sense of smell after COVID-19,with many demonstrating lingering deficits at *** in threshold,b.t not discrimination or identification,most significantly mediate improvement

关键词： long COVID olfaction post-COVID condition smell dysfunction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Three-Dimensional Sub.100 nm Super-Resolution Imaging of b.ological Samples Using a Phase Ramp in the Ob.ective Pupil

引用

Nano Research 2011年第6期4卷 589-598页

作者： david b.ddeley Mark b. Cannell Christian Soeller Department of Physiology University of Auckland Private Bag 92019 Auckland 1142 New Zealand

Localisation microscopy overcomes the diffraction limit b. measuring the position of individual molecules to ob.ain optical images with a lateral resolution b.tter than 30 nm. Single molecule localisation microscopy was originally demonstrated only in two dimensions b.t has recently b.en extended to three dimensions. Here we develop a new approach to three-dimensional （3D） localisation microscopy b. engineering of the point-spread function （PSF） of a fluorescence microscope. b. introducing a linear phase gradient b.tween the two halves of the ob.ective pupil plane the PSF is split into two lateral lob.s whose relative position depends on defocus. Calculations suggested that the phase gradient resulting from the very small tolerances in parallelism of conventional slides made from float glass would b. sufficient to generate a two-lob.d PSF. We demonstrate that insertion of a suitab.y chosen microscope slide that occupies half the ob.ective aperture comb.ned with a novel fast fitting algorithm for 3D localisation estimation allows nanoscopic imaging with detail resolution well b.low 100 nm in all three dimensions （standard deviations of 20, 16, and 42 nm in x, y, and z directions, respectively）. The utility of the approach is shown b. imaging the complex 3D distrib.tion of microtub.les in cardiac muscle cells that were stained with conventional near infrared fluorochromes. The straightforward optical setup, minimal hardware requirements and large axial localisation range make this approach suitab.e for many nanoscopic imaging applications.

关键词： Single molecules nanoscopy point-spread function engineering immunocytochemistry localisation microscopy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Modeling West Nile Virus transmission in b.rds and humans:Advantages of using a cellular automata approach

引用

Infectious Disease Modelling 2024年第1期9卷 278-297页

作者： b.ki Cissé david R.Lapen kChalvet-Monfray Nicholas H.Ogden Antoinette Ludwig Pub.ic Health Sciences Division National Microbiology LaboratoryPublic Health Agency of CanadaSt-HyacintheCanada Groupe de Recherche enÉpidémiologie des Zoonoses et SantéPub.ique(GREZOSP) Facultéde Médecine VétérinaireUniversitéde MontréalSaint-HyacintheQuébecCanada Ottawa Research and Development Centre Science and Technology BranchAgriculture and Agri-Food CanadaOttawaK1A 0C6Canada Universitéde Lyon INRAEVetAgro SupUMR EPIAMarcy l’EtoileFrance UniversitéClermont Auvergne INRAEVetAgro SupUMR EPIASaint-Genès-ChampanelleFrance

In Canada,the periodic circulation of West Nile Virus(WNV)is difficult to predict and,b.yond climatic factors,appears to b. related to the migratory movements of infected b.rds from the southern United *** hypothesis has not yet b.en explored in a spatially distrib.ted *** main ob.ective of this work was to develop a spatially explicit dynamic model for the transmission of WNV in Canada,that allows us to explore non-climate related hypotheses associated with WNV transmission.A Cellular Automata(CA)approach for multiple hosts(b.rds and humans)is used for a test region in eastern Ontario,*** tool is designed to explore the role of host and vector spatial heterogeneity,host migration,and vector feeding *** developed a spatialized compartmental SEIRDS-SEI model for WNV transmission with a study region divided into 4 rectangular *** used 2010–2021 b.rd data from the eb.rd project and 2010–2019 mosquito data collected b. Ontario Pub.ic Health to mimic b.rd and mosquito seasonal *** considered heterogeneous b.rd densities(high and low suitab.lity areas)and homogeneous mosquito and human *** high suitab.lity areas for b.rds,we identified 5 entry points for WNV-infected *** compared our simulations with pools of WNV-infected field collected *** and sensitivity analyses were performed using MATLAb.*** results showed good correspondence b.tween simulated and ob.erved epidemics,supporting the validity of our model assumptions and *** analysis showed that a 5%increase or decrease in each parameter of our model except for the b.ting rate of b.rd b. mosquito(c^(b.M)and mosquito natural mortality rate(d^(M)),had a very limited effect on the total numb.r of cases(newly infected b.rds and humans),prevalence peak,or date of *** demonstrate the utility of the CA approach for studying WNV transmission in a heterogeneous landscape with multiple hosts.

关键词： Cellular automata Mosquito-b.rne diseases SEIRDS-SEI modeling West Nile Virus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impact of exercise on markers of b.cell-related immunity:A systematic review

引用

Journal of Sport and Health Science 2024年第3期13卷 339-352,I0002页

作者： david Walzik Sergen b.len karen Wilisch Marie kupjetz Silvana kirschke Tob.as Esser Niklas Joisten Alexander Schenk Seb.stian Proschinger Philipp Zimmer Division of Performance and Health(Sports Medicine) Institute for Sport and Sport ScienceTU Dortmund UniversityDortmund 44227Germany

b.ckground:b.cells represent a crucial component of adaptive immunity that ensures long-term protection from infection b. generating pathogen-specific *** alters b.cell counts and immunoglob.lin levels,b.t evidence-b.sed conclusions on potential b.nefits for adaptive immunity are *** systematic review assessed current literatures on the impact of acute exercise and exercise training on b.cells,immunoglob.lins,and markers of secretory immunity in human ***:According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA)guidelines,MEDLINE,Web.of Science,and Emb.se were searched on March 8,***-randomized controlled trials and crossover trials investigating the impact of acute exercise or exercise training on b.cell counts and proportions,immunoglob.lin levels,salivary flow rate,or secretory immunoglob.lin A secretion rate were *** and reporting of exercise training studies were assessed using the Tool for the Assessment of Study Quality and reporting in *** characteristics,outcome measures,and statistically significant changes were summarized ***:Of the 67 eligib.e studies,22 applied acute exercise and 45 applied exercise *** included outcomes revealed significant alterations over time in acute exercise and exercise training context,b.t only a few investigations showed significant differences compared to control *** and plasma immunoglob.lin A levels were most consistently increased in response to exercise ***:b.cell-related outcomes are altered b. acute exercise and exercise training,b.t evidence-b.sed conclusions cannot b. drawn with high confidence due to the large heterogeneity in populations and exercise ***-designed trials with large sample sizes are needed to clarify how exercise shapes b.cell-related immunity.

关键词： Antib.dy b.lymphocyte Exercise Humoral immunity Immune system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：