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Bhlhe40 protects cochlear hair cell-like HEI-OC1 cells against H_(2)O_(2) ‑triggered oxidative injury

BIOCELL 2024年第6期48卷 991-999页

作者： LITING WEN XIAOXIA ZENG PEIXIONG CHEN dapeng zhao YANGYANG LI XIANHAI ZENG Department of Otorhinolaryngology Longgang Otorhinolaryngology Hospital and Shenzhen Key Laboratory of OtorhinolaryngologyShenzhen Institute of OtorhinolaryngologyShenzhenChina

Background:Cochlear hair cell injury is a common pathological feature of hearing loss.The basic helix-loop-helix family,member e40(Bhlhe40),a gene belonging to the basic helix-loop-helix(bHLH)family,exhibits strong transcriptional repression activity.Methods:Oxidative damage,in House Ear Institute-Organ of Corti 1(HEI-OC1)cells,was caused using hydrogen peroxide(H2O2).The Ad-Bhlhe40 particles were constructed to overexpress Bhlhe40 in HEI-OC1 cells.Various assays including cell counting kit-8(CCK-8),terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay(TUNEL),ﬂow cytometry,immunoﬂuorescence,and corresponding commercial kits were employed to investigate the impacts of Bhlhe40 on cell viability,apoptosis,oxidative stress levels,mitochondrial membrane potential and cellular senescence.Additionally,a dual-luciferase reporter assay was performed to conﬁrm the targeting of the histone deacetylases 2(Hdac2)by Bhlhe40.Results:The results revealed that Bhlhe40 was downregulated in H_(2)O_(2)-treated HEI-OC1 cells,but its overexpression improved cell viability and mitigated H_(2)O_(2)-induced oxidative injury in HEI-OC1 cells with increase of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx)activities and decrease of reactive oxygen species(ROS)levels.Besides,overexpression of Bhlhe40 suppressed H_(2)O_(2)-triggered cell senescence,as evidenced by the fact that the upregulation of P53,P21,and P16 in HEI-OC1 cells treated with H2O2 were all alleviated by Bhlhe40 overexpression.And we further veriﬁed that overexpression of Bhlhe40 could inhibit the expression of Hdac2,which may be related to the repression of Hdac2 transcription.Conclusion:This study suggests that Bhlhe40 plays a protective role against senescence and oxidative damage in cochlear hair cells exposed to H2O2.

关键词： Bhlhe40 Oxidative injury Cochlear hair cell Histone deacetylases 2

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Engineering nano-structures with controllable dimensional features on micro-topographical titanium surfaces to modulate the activation degree of M1 macrophages and their osteogenic potential

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Journal of Materials Science & Technology 2022年第1期96卷 167-178页

作者： Luxin Liang Qianli Huang Hong Wu Hao He Guanghua Lei dapeng zhao Kun Zhou State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China Foshan(Southern China)Institute for New Materials Foshan 528200China Department of Vascular Surgery The Second Xiangya HospitalCentral South UniversityChangsha 410011China Department of Orthopedics Xiangya HospitalCentral South UniversityChangsha 410008China College of Biology Hunan UniversityChangsha 410082China School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingapore 639798Singapore

Modulating the activation state and degree of macrophages still remains as a challenge for the topographical design of Ti-based implants.In this work,micro/nano-structured coatings were prepared on Ti substrates by micro-arc oxidation(MAO)and subsequent hydrothermal(HT)treatment.By varying the HT conditions,plate-like nano-structures with an average length of 80,440 or 780 nm were obtained on MAO-prepared micro-topographical surfaces.Depending on the dimensional features of nano-plates,the specimens were noted as Micro,Micro/Nano-180,Micro/Nano-440 and Micro/Nano-780,respectively.The in vitro results showed that the activation state and degree of macrophages could be effectively modulated by the micro/nano-structured surfaces with various dimensional features.Compared to the Micro surface,the Micro/Nano-180 surface activated both M1 and M2 phenotype in macrophages,while the Micro/Nano-440 and Micro/Nano-780 surfaces polarized macrophages to their M1 phenotype.The activation degree of M1 macrophages followed the trend:Micro

关键词： Titanium,Micro/nano-structures Macrophages activation Inflammatory response Osteogenesis

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Hand preference during bimanual coordinated task in northern pig-tailed macaques Macaca leonina

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Current Zoology 2016年第4期62卷 385-391页

作者： dapeng zhao Yuan WANG Xueyan WEI Tianjin Key Laboratory of Animal and Plant Resistance College of Life Sciences Tianjin Normal University Tianjin 300387 Wildlife Institute Beijing Forestry University Eco-Bridge Continental Beijing 100083 China

In humans, handedness is one defining characteristic regardless of cultures and ethnicity. Population-level right handedness is considered to be related with the evolution of left hemisphere for manual control and language. In order to further understand evolutionary origins of human cerebral lateralization and its behavioral adaptation, standardized measures on hand preference are required to make reliable comparison in nonhuman primate species. In this study, we present the first evidence on hand preference during bimanual coordinated tasks in northern pig-tailed macaques . The classical TUBE task was applied to examine hand preference among nine individuals from Tianjin Zoo of China. We recorded and made analysis on both frequency and bout data on manual laterality. The results consistently show that subjects displayed strong individual hand preferences, whereas no significant group-level handedness was found. There were no sex and age significant differences on both direction and strength of hand preference. The preferred to use the index finger to extract the baited food inside the tube. Our findings fill the knowledge gap on primate handedness, and efficiently affirm the robustness of the TUBE task as one efficient measure of hand preference in primates.

关键词： Macaca leonina bimanual coordination hand preference primate evolution.

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Crack mystery of the damaging Kumamoto earthquakes

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Science Bulletin 2016年第11期61卷 868-870页

作者： dapeng zhao Xin Liu Department of Geophysics Tohoku University College of Marine Geosciences Ocean University of China

The 2016 Kumamoto earthquake(M 7.3 in the Japan Meteorological Agency(JMA)scale)sequence wrote a new chapter of active seismotectonics and subduction dynamics in the Japan Islands.This earthquake sequence,

关键词：地震序列破坏性日本气象厅裂缝活动构造 Moto 动力学上地壳

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Sex-specific participation in inter-group conflicts within a multilevel society:the first evidence at the individual level

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Integrative Zoology 2013年第4期8卷 441-454页

作者： dapeng zhao Zhuoyue CHEN Baoguo LI Teresa ROMERO Tianjin Key Laboratory of Animal and Plant Resistance College of Life SciencesTianjin Normal UniversityTianjinChina College of Life Sciences Northwest UniversityXi’anChina Institute of Zoology Shaanxi Academy of SciencesXi’anChina Department of Cognitive and Behavioral Science Graduate School of Arts and SciencesUniversity of TokyoTokyoJapan Japan Society for the Promotion of Science ChiyodaTokyoJapan

Inter-group conflicts are common among many group-living animals and involve potentially complex motivations and interactions.Mammals living in multilevel societies offer a good opportunity to study inter-group conflicts.This study is the first to explore the function of sex-specific participation during inter-group conflicts within a multilevel society at the individual level.The Sichuan snub-nosed monkey(Rhinopithecus roxellana)is an endangered seasonal breeding species living in a multilevel society.From Sep 2007 to May 2008 we recorded 290 inter-group conflicts of a free-ranging provisioned band of R.roxellana in the Qinling Mountains of China to investigate the function of individual aggression during inter-group encounters.Our findings show that adult males were the main participants in inter-group conflicts,while females took part in them only occasionally.The male participation rate during the mating season,when adult females were estrous,was significantly higher than that during the non-mating season.Furthermore,males directed their aggression to other males,and directed more intense aggression towards bachelor males than towards other resident males.For both sexes,the participation rate as initiators was higher in the winter than that in the spring;and there was a significant positive correlation between group size and the participation rate as initiators.Our results suggest that inter-group aggression in Sichuan snub-nosed monkeys is linked to both mate defense and resource defense.

关键词： inter-group conflict mate defense multilevel society resource defense Rhinopithecus roxellana

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In vivo urinary compatibility of Mg-Sr-Ag alloy in swine model

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Bioactive Materials 2022年第1期7卷 254-262页

作者： Di Tie Norbert Hort Minfang Chen Renguo Guan Sviatlana Ulasevich Ekaterina V.Skorb dapeng zhao Yili Liu Patricia Holt-Torres Huinan Liu Engineering Research Center of Continuous Extrusion Ministry of EducationDalian Jiaotong UniversityDalian116028China Magnesium Innovation Center Helmholtz-Zentrum GeesthachtD-21502GeesthachtGermany School of Materials Science and Engineering Tianjin University of TechnologyTianjin300384China Infochemistry Scientific Center ITMO UniversitySt.Petersburg192007Russia College of Biology Hunan University410082ChangshaChina Department of Urology China Medical UniversityShenyang110084China Department of Bioengineering Bourns College of EngineeringUniversity of California at RiversideCA92521USA

A biodegradable metallic ureteral stent with suitable mechanical properties and antibacterial activity remains a challenge.Here we reveal the scientific significance of a biodegradable Mg-Sr-Ag alloy with a favorable combination of balanced mechanical properties,adjustable indwelling time in urinary tract and evident antibacterial activity via in vivo experiments in a swine model.Attributed to the rheo-solidification process,equiaxial microstructure and significantly refined grains(average grain size:27.1μm)were achieved.Mg17Sr2 and Mg4Ag were found as the primary precipitates in the matrix,due to which the alloy obtained ca.111%increase in ultimate tensile strength in comparison to pure magnesium.Both the in vitro and in vivo results demonstrated the satisfactory biocompatibility of the alloy.Histological evaluation and bioindicators analysis suggested that there was no tissue damage,inflammation and lesions in the urinary system caused by the degradation process.The stent also improved the post-operative bladder functions viewed from the urodynamic results.Our findings highlight the potential of this alloy as antibacterial biodegradable urinary implant material.

关键词： In vivo Magnesium alloy Biodegradable metals Mechanical properties Urinary compatibility

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Postconflict behavior among female Sichuan snub-nosed monkeys Rhinopithecus roxellana within one-male units in the Qinling Mountains,China

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Current Zoology 2010年第2期56卷 222-226页

作者： Jian ZHANG dapeng zhao Baoguo LI College of Life Sciences Northwest University Xi＇an 710069 China Institute of Zoology Shaanxi Academy of Sciences Xi＇an 710069 China Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

For group-living primates, the information on postconflict management is crucial for understanding primate competitionand cooperation. However, such information is poorly known for snub-nosed monkeys, especially for wild populations. Inthis study, from September 2007 to June 2008, we investigated postconflict behavior among adult females Sichuan snub-nosedmonkeys Rhinopithecas roxellana within one-male units in a wild, provisioned group in the Qinling Mountains of China bymeans of the time-rule method and the PC-MC method. We obtained a total of 81 PC-MC pairs and each individual was involvedin only 0.004 aggressive behavior per observation hour. The first affiliative behavior was more likely to occur within the firstminute after a conflict. The postconflict affiliative behaviors most often seen were contact-sit, embrace and grooming. The affiliativecontacts between adult females occur due to selective attraction, i.e. reconciliation. The pattern of postconflict affiliationdemonstrates that the R. roxellana belongs to a tolerant

关键词：川金丝猴秦岭山脉性行为单位女性中国灵长类动物 MC方法

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Sex differences in anogenital distances and digit ratios in wild David＇s rock squirrels Sciurotamias davidianus

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Current Zoology 2014年第2期60卷 180-185页

作者： dapeng zhao Zhuoyue CHEN Baoguo LI Tianjin Key Laboratory of Animal and Plant Resistance College of Life Sciences Tianjin Normal University Tianjin 300387China College of Life Sciences Northwest University Xi'an 710069 China

The prenatal hormonal environment plays an important role in organizing sex differences in the morphology, physiology and behavior of humans as well as other vertebrates. Currently, all related research on rodents has been focused on Myo-morpha. This study presents data on sex differences in the anogenital distance (AGD) and the ratio of the second to the fourth digit length (2D:4D) from 44 wild David’s rock squirrelsSciurotamias davidianuscaptured from the Qinling Mountains, China. This was the first study of a species from Sciuromorpha. The AGD as well as body mass are sexually dimorphic. There are no sex differences or lateral asymmetry in 2D:4D. Significant correlation was not found between AGD and 2D:4D for any paw. The findings indicate that 2D:4D may not be fixed or influenced by the prenatal steroidal environment in David’s rock squirrels.

关键词：性别差异岩松鼠生殖器距离肛门野生和数脊椎动物

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Inhibition of HeLa cell growth by doxorubicin-loaded and tuftsinconjugated arginate-PEG microparticles

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Bioactive Materials 2018年第1期3卷 48-54页

作者： Tianmu Hu Anwar Saeed Ahmed Qahtan Lei Lei Zhixin Lei dapeng zhao Hemin Nie Institute of Bionanotechnology and Tissue Engineering College of Life SciencesHunan UniversityChangsha410082China Department of Orthodontics Xiangya Stomatological HospitalCentral South UniversityChangsha410008China Changsha DMY Medical Technology Co. LtdChangsha410005China Shenzhen Research Institute of Hunan University Nanshan Hi-new Technology and Industry ParkShenzhen518057China

In order to improve the release pattern of chemotherapy drug and reduce the possibility of drug resistance,poly(ethylene glycol amine)(PEG)-modified alginate microparticles(ALG-PEG MPs)were developed then two different mechanisms were employed to load doxorubicin(Dox):1)forming Dox/ALGPEG complex by electrostatic attractions between unsaturated functional groups in Dox and ALG-PEG;2)forming Dox-ALG-PEG complex through EDC-reaction between the amino and carboxyl groups in Dox and ALG,respectively.Additionally,tuftsin(TFT),a natural immunomodulation peptide,was conjugated to MPs in order to enhance the efficiency of cellular uptake.It was found that the Dox-ALG-PEGTFT MPs exhibited a significantly slower release of Dox than Dox/ALG-PEG-TFT MPs in neutral medium,suggesting the role of covalent bonding in prolonging Dox retention.Besides,the release of Dox from these MPs was pH-sensitive,and the release rate was observably increased at pH 6.5 compared to the case at pH 7.4.Compared with Dox/ALG-PEG MPs and Dox-ALG-PEG MPs,their counterparts further conjugated with TFT more efficiently inhibited the growth of HeLa cells over a period of 48 h,implying the effectiveness of TFT in enhancing cellular uptake of MPs.Over a period of 48 h,Dox-ALG-PEG-TFT MPs inhibited the growth of HeLa cells less efficiently than Dox/ALG-PEG-TFT MPs but the difference was not significant(p>0.05).In consideration of the prolonged and sustained release of Dox,Dox-ALGPEG-TFT MPs possess the advantages for long-term treatment.

关键词： Controlled release Tuftsin Cellular uptake Chemotherapy

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利用不规则网格地震层析成像研究2008年日本岩手地震及岛弧火山活动(英文)

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地学前缘 2013年第2期20卷 155-171页

作者： Wei WEI dapeng zhao Department of Geophysics Tohoku University Key Laboratory of Active Tectonics and Volcano Institute of GeologyChina Earthquake Administration

为了深入了解日本东北俯冲带的地震构造及火山活动,利用布设在日本列岛上密集地震台网所记录到的高质量浅震及深震到时数据,反演求得了该区域地壳及上地幔的三维P波和S波速度结构。为了最大程度地利用地震数据提取模型空间中更为精细的... 详细信息

为了深入了解日本东北俯冲带的地震构造及火山活动,利用布设在日本列岛上密集地震台网所记录到的高质量浅震及深震到时数据,反演求得了该区域地壳及上地幔的三维P波和S波速度结构。为了最大程度地利用地震数据提取模型空间中更为精细的速度结构信息,采用不规则网格模型采进行地震层析成像反演。所得的高分辨率成像结果清晰地显示,2008年岩手地震(M 7.2)位于高低速异常的转换区,而且震源区的地壳介质非均匀性极强。在震源区的下地壳及上地幔顶部存在着明显的低速异常,可能代表了岛弧岩浆和流体在该深度处的储集。研究结果表明,2008年岩手地震的产生受到了来自上地幔楔的岩浆和流体的影响,且这些岩浆和流体与俯冲太平洋板块的脱水作用有着密切的联系。

关键词： 2008年岩手地震不规则网格参数化岩浆与流体板块脱水低频地震

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