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Journal of Pharmaceutical Analysis 2021年第6期11卷 783-790页

作者： Shiya Liu Zhiyuan Wang daoqi zhu Jiabin Yang Dandan Lou Ruijiao Gao Zetai Wang Aiwu Li Ying Lv Qin Fan School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou510515China Nan Fang Hospital Guangzhou510515China

Shengmai Yin(SMY)is a Chinese herbal decoction that effectively alleviates the side effects of radiotherapy in various cancers and helps achieve radiotherapy’s clinical *** this study,we explored the interaction mechanism among SMY,DNA methylation,and nasopharyngeal carcinoma(NPC).We identified differences in DNA methylation levels in NPC CNE-2 cells and its radioresistant cells(CNE-2R)using the methylated DNA immunoprecipitation array and found that CNE-2R cells showed genomewide changes in methylation status towards a state of *** may restore its original DNA methylation status,and thus,enhance ***,we confirmed that the differential gene Tenascin-C(TNC)was overexpressed in CNE-2R cells and that SMY downregulated TNC *** downregulation of TNC inhibited NPC cell radiation resistance,migration,and ***,we found that TNC was hypomethylated in CNE-2R cells and partially restored to a hypermethylated state after SMY *** methyltransferases 3 a may be the key protein in DNA methylation of TNC.

关键词： Nasopharyngeal carcinoma DNA methylation Radiosensitization Shengmai Yin

Clinical study of gamma ray whole body therapeutic system for malignant tumors (report of 643 cases)

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The Chinese-German Journal of Clinical Oncology 2008年第2期7卷 115-117页

作者： Guanghua Zhou daoqi zhu Jiali Su Limin Liu Center of Oncology Radiotherapy The No. 163 Hospital of PLA Changsha 410003 China

Objective： To evaluate the feasibility of the whole body gamma knife in the treatment of malignant solid tumors. Methods： 643 patients diagnosed by pathology or cytology were treated with the whole body gamma knife. They received a total dose from 40-60 Gy/2-3 w, 3-10 Gy/fraction, 5 fractions/week. Results： The rates of response, complete remission, partial remission, no remission, and tumor progression were 94.6%, 39.2%, 55.4%, 5.1% and 0.3%, respectively. Conclusion： Whole body γ-radiation system （y-knife） was an effective method in treatment of patients with malignant tumor.

关键词： the whole body gamma knife tumor radiotherapy tomography

Clinical observation of colon carcinoma treated with Boerning capsules plus FOLFOX4 regimen

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The Chinese-German Journal of Clinical Oncology 2007年第4期6卷 328-330页

作者： Ping Liu Jianhui Liu daoqi zhu Jin＇an Ma Chunhong Hu Department of Oncology the Second Hospital of Xiangya Central South University Changsha 410011 China Department of Oncology the 163 Hospital the People＇s Liberation Army of Chinal Changsha 410003 China

Objective:To evaluate the effects and adverse reactions of Boerning capsules plus FOLFOX4 regimen in clinical treatment for colon ***:Total patients with colon carcinoma were randomly divided into two groups from January 2005 to May 2006:group A(45 patients)were received Boerning capsules plus FOLFOX4 regimen treatment,and group B(49 patients)were received FOLFOX4 regimen treatment ***:Boerning capsules could obviously improve the life quality of the colon carcinoma *** weight gain in group A was higher than that of group *** significant existed in the body immune function changes(T cell subgroup).But it could ameliorate the adverse reactions which induced by the ***:Boerning capsules can improve the life quality of the colon carcinoma patients and ame- liorate the symptoms and sign which induced by the chemotherapy.

关键词： Boerning capsules FOLFOX4 regimen colon carcinoma

miR-129-5p在人脑胶质瘤组织中表达下调的表观遗传学机制

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中国肿瘤生物治疗杂志 2016年第2期23卷 201-205页

作者：杨妤苏加利朱道奇申良方中南大学湘雅医院肿瘤科湖南长沙410008 解放军第163医院肿瘤科湖南长沙410003

目的:检测miR-129成熟体miR-129-5p在人脑胶质瘤组织中的表达特点,探讨人脑胶质瘤组织中miR-129-2表达异常是否与miR-129-2启动子甲基化有关。方法:收集湘雅医院神经外科2013年10-12月收治的胶质瘤患者肿瘤组织21例、脑外伤患者非肿瘤... 详细信息

目的:检测miR-129成熟体miR-129-5p在人脑胶质瘤组织中的表达特点,探讨人脑胶质瘤组织中miR-129-2表达异常是否与miR-129-2启动子甲基化有关。方法:收集湘雅医院神经外科2013年10-12月收治的胶质瘤患者肿瘤组织21例、脑外伤患者非肿瘤脑组织标本21例。Real-time PCR检测脑胶质瘤组织和非肿瘤脑组织、脑胶质瘤细胞株和正常脑胶质细胞株中miR-129-5p的表达;甲基化特异性PCR(methylation-specific PCR,MSP)检测胶质瘤组织中miR-129-2基因启动子甲基化的情况;亚硫酸氢盐硫化测序PCR(bisulfite-sequencing PCR,BSP)分析经甲基化转移酶抑制剂5-氮杂-2'-脱氧胞苷(5-aza-2'-deoxycitydine,5-Aza-d C)处理后的胶质瘤细胞miR-129-2基因启动子甲基化水平的变化,同时Real-time PCR检测miR-129-5p的表达变化。结果:miR-129-5p在人脑胶质瘤组织和细胞中表达显著低于非肿瘤脑组织和正常脑胶质细胞(P<0.05),胶质瘤组织中miR-129-2基因的启动子甲基化率显著高于正常脑组织(P<0.05),使用5-Aza-d C去甲基化处理脑胶质瘤细胞后miR-129-2基因的启动子甲基化程度降低、miR-129-5p表达显著升高(P<0.05)。结论:miR-129-5p在脑胶质瘤中显著低表达,miR-129-2启动子甲基化是导致其低表达的机制之一。

关键词： miR-129-5p 脑胶质瘤启动子甲基化

Wnt/β-catenin通路相关基因参与穴位敷贴修复缺血性脑损伤机制

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中华中医药学刊 2018年第11期36卷 2651-2654,I0009页

作者：朱道琦刘雅倩刘华聪张东淑南方医科大学中医药学院针灸教研室

目的：穴位敷贴治疗脑缺血为＂靳三针＂特色疗法,本实验在前期研究证实穴位敷贴对脑缺血具有神经保护作用的基础上,探讨Wnt/β-catenin通路基因参与其修复缺血性脑损伤的可能机制。方法：制备安宫牛黄贴（药物组成以郁金、黄芩、栀子、... 详细信息

目的：穴位敷贴治疗脑缺血为＂靳三针＂特色疗法,本实验在前期研究证实穴位敷贴对脑缺血具有神经保护作用的基础上,探讨Wnt/β-catenin通路基因参与其修复缺血性脑损伤的可能机制。方法：制备安宫牛黄贴（药物组成以郁金、黄芩、栀子、冰片、麝香等为主）,实验动物分为正常组、模型组、药物组及穴位敷贴（高、中、低剂量）组,制备大脑中动脉局灶性脑缺血模型（MCAO）,每组分为3个亚组（脑缺血后6 h、3 d、7 d）。采用RT-qPCR检测Wnt3、Dishevelled、Caspase-3、Survivin mRNA;Western-bolt检测β-catenin、GSK-3β蛋白表达。结果：穴位敷贴治疗后,穴位敷贴中剂量组大鼠神经行为学评分明显降低（P〈0.05）,大鼠缺血侧脑组织Wnt3、Dishevelled、Survivin mRNA、β-catenin蛋白表达不同程度增加,Caspase-3mRNA、GSK-3β蛋白不断下降,与模型组相比具有统计学差异（P〈0.01或P〈0.05）,以穴位敷贴中剂量组上述变化较为显著。结论：Wnt/β-catenin通路参与穴位敷贴防治缺血性脑损伤的可能机制为上调核心分子Wnt3、Dishevelled、β-catenin,激活靶基因Survivin,下调抑制因子GSK-3β、Caspase-3表达。

关键词： Wnt/β-catenin通路基因穴位敷贴脑缺血

鼻咽癌斑马鱼移植瘤模型的构建及姜黄素对CNE-2细胞的抑制作用

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吉林大学学报（医学版） 2022年第1期48卷 9-17页

作者：王泽泰娄丹丹彭燕朱道琦李爱武宫凤英吕英范钦南方医科大学中医药学院分子生物学教研室广东广州510515 南方医科大学南方医院古中医科广东广州510515

目的:建立人鼻咽癌斑马鱼异种移植瘤模型,探讨姜黄素在体内外对鼻咽癌的抑制作用,为阐明姜黄素抗鼻咽癌的分子机制提供依据。方法:将鼻咽癌CNE-2细胞分为对照组和细胞膜红色荧光染色剂CM-Dil(CM-Dil)组,连续5 d进行细胞计数,并观察荧光... 详细信息

目的:建立人鼻咽癌斑马鱼异种移植瘤模型,探讨姜黄素在体内外对鼻咽癌的抑制作用,为阐明姜黄素抗鼻咽癌的分子机制提供依据。方法:将鼻咽癌CNE-2细胞分为对照组和细胞膜红色荧光染色剂CM-Dil(CM-Dil)组,连续5 d进行细胞计数,并观察荧光强度变化。采用显微注射法构建鼻咽癌斑马鱼异种移植瘤模型,将斑马鱼胚胎分为对照组、PBS组和模型组,对照组斑马鱼胚胎不予处理,PBS组斑马鱼胚胎注射10 nLPBS缓冲液,模型组斑马鱼胚胎注射10 nLCNE-2细胞悬液,孵育培养后统计各组斑马鱼存活数,采用宏观体式显微镜观察并定量分析模型组斑马鱼体内移植瘤荧光强度,HE染色观察对照组和模型组斑马鱼移植瘤组织形态表现。将受精后天数(dpf)为3的斑马鱼胚胎暴露于含不同浓度(0、0.625、1.250、2.500、5.000、7.500和10.000μmol·L^(-1))姜黄素的饲养水中,观察斑马鱼死亡数和畸形数并计算斑马鱼死亡率,筛选姜黄素体内用药浓度。采用0、0.625、1.250、2.500和5.000μmol·L^(-1)姜黄素作用于斑马鱼鼻咽癌模型48 h,检测移植瘤生长和转移情况。0、10和20μmol·L^(-1)姜黄素作用CNE-2细胞24 h,CCK-8法和划痕实验检测细胞增殖率和迁移率。结果:与对照组比较,CM-Dil组CNE-2细胞数差异无统计学意义(P>0.05)。实验第5天,与对照组比较,PBS组斑马鱼存活数差异无统计学意义(P>0.05);与对照组和PBS组比较,模型组斑马鱼存活数明显减少(P<0.01)。模型组斑马鱼体内红色荧光随着时间的延长而增强;与注射后第1天比较,注射后第5天模型组斑马鱼体内红色荧光强度明显增强(P<0.05)。HE染色,模型组斑马鱼切片中的卵黄囊有CNE-2细胞,提示造模成功。浓度小于或等于5.000μmol·L^(-1)姜黄素组斑马鱼无死亡及明显毒性反应。与对照组(0μmol·L^(-1)姜黄素组)比较,2.500和5.000μmol·L^(-1)姜黄素组斑马鱼体内移植瘤荧光强度明显降低(P<0.05或P<0.01),5.000μmol·L^(-1)姜黄素组发生头尾部转移的斑马鱼数量明显减少(P<0.05)。CCK-8法检测和划痕实验,与对照组(0μmol·L^(-1)姜黄素组)比较,10和20μmol·L^(-1)姜黄素组CNE-2细胞增殖率和迁移率明显降低(P<0.05)。结论:人鼻咽癌CNE-2细胞可以成功植入斑马鱼体内并构建鼻咽癌斑马鱼模型,姜黄素在体内外均能抑制鼻咽癌细胞的增殖和转移,具有良好的抗肿瘤前景。

关键词：姜黄素鼻咽肿瘤移植瘤斑马鱼细胞增殖率细胞迁移率

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生脉注射液对人鼻咽癌CNE-2细胞抗拒株的放射增敏作用

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世界科学技术-中医药现代化 2020年第3期22卷 804-809页

作者：刘诗雅朱道琦杨家彬娄丹丹高瑞娇吕英范钦南方医科大学中医药学院分子生物实验室广州510515 南方医科大学南方医院古中医科广州510515

目的探讨生脉注射液对人鼻咽癌CNE-2细胞抗拒株及其裸鼠移植瘤的放射增敏作用以及对STAT3、p-STAT3蛋白表达的影响。方法通过细胞毒性实验筛选生脉注射液的最佳浓度,克隆形成实验检测用药前后放射敏感性;建立人鼻咽癌裸鼠移植瘤模型,荷... 详细信息

目的探讨生脉注射液对人鼻咽癌CNE-2细胞抗拒株及其裸鼠移植瘤的放射增敏作用以及对STAT3、p-STAT3蛋白表达的影响。方法通过细胞毒性实验筛选生脉注射液的最佳浓度,克隆形成实验检测用药前后放射敏感性;建立人鼻咽癌裸鼠移植瘤模型,荷瘤鼠随机分为空白组,生脉注射液低、高剂量组,生脉注射液低、高剂量联合照射组,照射组和姜黄素联合照射对照组。计算肿瘤体积抑制率和抑瘤率。蛋白免疫印迹法检测基因表达。结果生脉注射液给药后,CNE-2R细胞放射敏感性增加;与单纯照射组比较,高剂量联合照射组肿瘤体积抑制率及抑瘤率明显升高,其STAT3、p-STAT3表达亦显著下调。结论生脉注射液对人鼻咽癌CNE-2细胞抗拒株CNE-2R及其裸鼠移植瘤具有放射增敏作用,其增敏作用可能与抑制STAT3表达与活化相关。

关键词：鼻咽癌裸鼠移植瘤生脉注射液放射增敏

Modification of Maxwell model for conductivity prediction of carbon nanotubes-filled polymer composites with tunneling effect

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Applied Mathematics and Mechanics(English Edition) 2025年第1期46卷 25-36页

作者： Jue zhu Longyuan LI Ningtao zhu Zhejiang Provincial Engineering Research Center for the Safety of Pressure Vessel and Pipeline Key Laboratory of Impact and Safety Engineering (Ningbo University) Ministry of Education School of Engineering Computing and Mathematics University of Plymouth Xi'an Zhanshi Testing & Engineering Co.Ltd.

Carbon nanotubes(CNTs) have garnered great attention in recent years due to their outstanding electrical, thermal, and mechanical properties. The incorporation of small amounts of CNTs in polymers can substantially improve the sensitivity of the polymer's electrical conductivity. This paper presents a modified Maxwell model to evaluate the electrical conductivity of CNTs-filled polymer composites by introducing a transition zone to account for the tunneling effect. In this modified Maxwell model, the CNTs-filled polymer composite is modeled as a three-phase composite, consisting of a matrix(polymer), inclusions(CNTs), and a transition zone(tunneling zone). The effective electrical conductivity(EEC) of the composite is calculated based on the volume fractions and electrical conductivities of the matrix, inclusions, and transition zone. The model's validity is confirmed through the use of available test data, which demonstrates its capability to accurately capture the nonlinear conductivity behavior observed in CNTs-polymer composites. This study offers valuable insights into the design of high-performance conductive polymer nanocomposites, and enhances the understanding of electrical conduction mechanisms in CNT-dispersed polymer composites.

关键词：

穴位敷贴对急性脑缺血大鼠神经元损伤的保护作用

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中医学报 2014年第6期29卷 753-755页

作者：张东淑陈静刘远亮朱道琦南方医科大学广东广州510515

目的:探讨穴位敷贴对大鼠急性脑缺血后神经元损伤的影响。方法:采用线栓法制备大鼠脑缺血模型;治疗前后分别对大鼠进行神经行为学症状评分,HE染色法观察各组大鼠脑缺血后神经元损害情况。结果:治疗后,穴位敷贴中剂量组、高剂量组及阳性... 详细信息

目的:探讨穴位敷贴对大鼠急性脑缺血后神经元损伤的影响。方法:采用线栓法制备大鼠脑缺血模型;治疗前后分别对大鼠进行神经行为学症状评分,HE染色法观察各组大鼠脑缺血后神经元损害情况。结果:治疗后,穴位敷贴中剂量组、高剂量组及阳性药物组神经行为学评分均明显减低(P<0.05);与正常组比较,模型组大鼠脑组织神经细胞脱失、水肿及变形明显;各治疗组大鼠神经细胞脱失、水肿及变形现象较模型组有不同程度减轻。结论:穴位敷贴可改善急性脑缺血大鼠神经行为学症状,对神经元损伤有一定的保护作用。

关键词：急性脑缺血穴位敷贴神经元损伤大鼠

Impacts of the annual cycle of background SST in the tropical Pacific on the phase and amplitude of ENSO

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 12-17页

作者： Song Jiang Congwen zhu Ning Jiang State Key Laboratory of Severe Weather(LASW) Chinese Academy of Meteorological SciencesBeijingChina College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijingChina

The dominant annual cycle of sea surface temperature(SST)in the tropical Pacific exhibits an antisymmetric mode,which explains 83.4%total variance,and serves as a background of El Niño-Southern Oscillation(ENSO).However,there is no consensus yet on its anomalous impacts on the phase and amplitude of *** on data during 1982-2022,results show that anomalies of the antisymmetric mode can affect the evolution of ENSO on the interannual scale via Bjerknes feedback,in which the positive(negative)phase of the antisymmetric mode can strengthen El Niño(La Niña)in boreal winter via an earlier(delayed)seasonal cycle transition and larger(smaller)annual *** magnitude of the SST anomalies in the equatorial eastern Pacific can reach more than±0.3◦C,regulated by the changes in the antisymmetric mode based on random sensitivity *** reveal the spatial pattern of the annual cycle associated with the seasonal phase-locking of ENSO evolution and provide new insight into the impact of the annual cycle of background SST on ENSO,which possibly carries important implications for forecasting ENSO.

关键词： Annual cycle SST anomaly Antisymmetric mode ENSO