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Involvement of methylation-associated silencing of formin 2 in colorectal carcinogenesis

World Journal of Gastroenterology 2018年第44期24卷 5013-5024页

作者： dao-jiang Li Zhi-Cai Feng Xiao-Rong Li Gui Hu Department of Gastro-intestinal Surgery the Third Xiangya Hospital Central South University Department of Burns and Plastic Surgery the Third Xiangya Hospital Central South University

AIM To investigate whether promoter methylation is re-sponsible for the silencing of formin 2 （ FMN2） in colorectal cancer （CRC） and to analyze the association between FMN2 methylation and *** frst identifed the expression levels and methylation levels of FMN2 in large-scale human CRC expression data-sets, including GEO and TCGA, and analyzed the relation-ship between the expression and methylation levels. Then, the methylation levels in four CpG regions adjacent to the FMN2 promoter were assessed by MethylTarget？ assays in CRC cells and in paired colorectal tumor samples and adjacent nontumor tissue samples. Furthermore, we inhibited DNA methylation in CRC cells with 5-Aza-2’-deoxycytidine and assessed the expression of FMN2 by qRT-PCR. Last, the association between FMN2 methylation patterns and clinical indicators was *** statistically significant downregulation of FMN2 ex-pression in large-scale human CRC expression datasets was found. Subsequent analysis showed that a high frequency of hypermethylation occurred in the FMN2 gene promoter in CRC tissues; operating characteristic curve analysis revealed that FMN2 gene methylation had a good capability for discriminating between CRC and nontumor tissue samples （AUC = 0.8432, P 〈 0.0001）. MethylTarget？ assays showed that CRC cells and tissues displayed higher methylation of these CpG regions than nontumor tissue samples. Correlation analysis showed a strong inverse correlation between methylation and FMN2 expression, and the inhibition of DNA methylation with 5-Aza significantly increased endogenous FMN2 expression. Analysis of the association between FMN2 methylation patterns and clinical indicators showed that FMN2 methylation was signifcantly associated with age, N stage, lymphovascular invasion, and pathologic tumor stage. Notably, the highest methylation of FMN2 occurred in tissues from cases of early-stage CRC, including cases with no regional lymph node metastasis （N0）, cases in stag

关键词： Formin 2 Colorectal cancer Methylation Methylation-associated silencing Early-stage cancer

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Bile acids in serum and bile of patients with cholesterol gallstone

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World Journal of Gastroenterology 1998年第1期4卷 87-89页

作者： HAN Tian-Quan ZHANG Sheng-dao TANG Wen-Hao jiang Zhao-Yan Department of Surgery Ruijin Hospital Shanghai Second Medical University Shanghai 200025 China Bengbu Medical College China Shanghai Second Medical University China Department of Surgery Ruijin Hospital Shanghai Second Medical University 197 Ruijin Erlu Shanghai 200025 China Department of Surgery Affiliated Hospital Nanjing Railway Medical College Nanjing 210009 China

IM To analyze serum bile acids and biliary lipids of patients with cholesterol gallstone(CS) and explore the relationship between deoxycholic acid (DCA) and CS disease.METHODS Analysis of bile acids in serum was done with gaschromatography in two groups: CS group (n=151) and control group (n=256). Serum bile acids and biliary lipids were also studied in 90 matched samples..RESULTS The serum DCA was 0955μmol/L±0078μmol/L in CS group, which was more than that of control group (0696μmol/L±0047μmol/L), Phe ratio of DCA/chenodeoxycholic acids (CDCA) was 176±030 in CS group, about two times that in control group (092±014). The mole percent of DCA in bile was positively related to cholesterol saturation index (CSI) (Phe mole percent of CDCA in bile negatively to CSI (P=001). There was correlation between the mole percent of DCA, CDCA and cholic acid in bile and in serum.CONCLUSION It is suggested that DCA is lithogenic and the increased amount of DCA or the ratio of DCA/CDCA in serum may be one of the features of cholesterol gallstone patients.

关键词： cholesterol gallstone bile acids/analysis deoxycholic acids/analysis chromatography,gas

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Global Longitudinal Strain at Rest for Detection of Coronary Artery Disease in Patients without Diabetes Mellitus

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Current Medical Science 2018年第3期38卷 413-421页

作者： Hou-juan ZUO Xiu-ting YANG Qi-gong LIU Yan ZHANG he-song ZENG jiang-tao YAN dao-wen WANG Hong WANG Department of Cardiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Cardiology Binzhou Medical University Hospital Binzhou 256600 China

Global longitudinal strain （GLS） at rest on two-dimensional speckle tracking echocardiography （2D STE） was demonstrated to help detect coronary artery disease （CAD）. However, the optimal cut-off point of GLS and its diagnostic power for detecting critical CAD in non-diabetes mellitus （DM） patients are unknown. In the present study, 211 patients with suspected CAD were prospectively included, with DM patients excluded. All patients underwent echocardiography and subsequently coronary angiography within 3 days. Left ventricular （LV） GLSs were quantified by 2D STE. Territorial peak systolic longitudinal strains （TLSs） were calculated based on the perfusion territories of the 3-epicardial coronary arteries in a 17-segment LV model. Critical CAD was defined as an area stenosis _〉70% in _〉1 epicardial coronary artery （_〉50% in left main coronary artery）. Totally 145 patients were diagnosed as having critical CAD by coronary angiography. Significant differences were observed in all strain parameters between patients with and without critical CAD. The area under the receiver operating charcteristic （ROC） curve （AUC） for GLS in the detection of left main （LM） or threevessel CAD was 0.875 at a cut-off value of-19.05% with sensitivity of 78.1% and specificity of 72.7%, which increased to 0.926 after exclusion of apical segments （cut-off value -18.66%; sensitivity 84.4% and specificity 81.8%）. The values of TLSs were significantly lower in regions supplied by stenotic arteries than in those by non-stenotic arteries. The AUC for the TLSs to identify critical stenosis of left circumflex （LCX） artery, left anterior descending （LAD） artery and right coronary artery （RCA）, in order of diagnostic accuracy, was 0.818 for LCX, 0.764 for LAD and 0.723 for RCA, respectively. In conclusion, in non-DM patients with suspected CAD, GLS assessed by 2D STE is an excellent predictor for LM or three-vessel CAD with high diagnostic accuracy, and a higher cut-off point than reported before should

关键词： two-dimensional strain coronary heart disease global longitudinal strain territorial longitudinal strain

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Electrodeposition of functionally graded Ni-W/Er2O3 rare earth nanoparticle composite film

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International Journal of Minerals,Metallurgy and Materials 2020年第6期27卷 818-829页

作者： Guo-quan Lai Hong-zhong Liu Bang-dao Chen Dong Niu Biao Lei Wei-tao jiang State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049China

Multi-layered functionally graded(FG)structure Ni-W/Er2O3 nanocomposite films were prepared by continuously changing the deposition parameters,in which the Er2O3 and W contents varied with *** microstructure and chemical composition of the electrodeposited Ni-W/Er2O3 films were determined by scanning electron microscopy(SEM)and energy-dispersive X-ray spectroscopy(EDS).The anti-corrosion and wear properties of the electrodeposition films were investigated by electrochemical measurement and ball-on-disk friction *** microhardness distribution of the cross section of nanocomposites was measured by *** results showed that with decreasing agitation rate or increasing average current density,the contents of Er2O3 nanoparticles and tungsten were distributed in a gradient along the thickness,and the contents on the surface were *** comparison,FG Ni-W/Er2O3 films had better anti-corrosion and wear properties than the uniform Ni-W/Er2O3 *** force microscopy(AFM)and profilometry measurements indicated that Er2O3 nanoparticles had an effect on the surface roughness.

关键词： nickel-tungsten electrodeposition functionally graded material nanocomposite erbium oxide nanoparticle

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不同保鲜方式下鲜食玉米品质变化规律的研究

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食品安全质量检测学报 2024年第8期15卷 22-32页

作者：赵琳陈玉骆乐谈石江王道泽杭州市农业科学研究院农作物(生态)研究所杭州310024 杭州市临安区科学技术局农村与社会发展科杭州311300 杭州市乡村振兴服务中心杭州310020

目的探究不同保鲜方式下鲜食玉米各品质指标的变化规律。方法针对不同类型鲜食玉米(甜玉米、糯玉米和甜糯玉米),选取常温(25℃)、保鲜温度(4℃)2种温度下真空包装(蒸煮、非蒸熟)、普通保鲜袋、特制袋子、仪器等不同处理,通过试剂盒测定... 详细信息

目的探究不同保鲜方式下鲜食玉米各品质指标的变化规律。方法针对不同类型鲜食玉米(甜玉米、糯玉米和甜糯玉米),选取常温(25℃)、保鲜温度(4℃)2种温度下真空包装(蒸煮、非蒸熟)、普通保鲜袋、特制袋子、仪器等不同处理,通过试剂盒测定不同处理下氨基酸、淀粉、糖类的变化趋势和规律。结果0 d时,甜玉米(钱江甜1号)蒸煮后氨基酸含量升高,而糯玉米(晋糯20号)和甜糯玉米(钱江糯3号)蒸煮后氨基酸含量降低;甜玉米(钱江甜1号)蒸煮后可溶性糖和葡萄糖含量变高,糯玉米(晋糯20号)蒸煮后蔗糖含量变高,甜糯玉米(钱江糯3号)蒸煮后果糖、葡萄糖和蔗糖含量变高;短期保鲜时,可以采用4℃真空和4℃特制袋子+保鲜剂+吸水纸两种处理。结论甜玉米、糯玉米和甜糯玉米在不同保鲜时间、不同处理间各指标的变化趋势不同,短期保鲜时,4℃真空不需额外添加保鲜剂,而4℃特制袋子+保鲜剂处理中一定要添加吸水纸,否则保鲜效果将变差。

关键词：鲜食玉米保鲜贮藏筛选

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DNA demethylases remodel DNA methylation in rice gametes and zygote and are required for reproduction

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Molecular Plant 2021年第9期14卷 1569-1583页

作者： Shaoli Zhou Xue Li Qian Liu Yu Zhao Wei jiang Anqi Wu dao-Xiu Zhou National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University430070 WuhanChina Institute of Plant Science Paris–Saclay(IPS2) CNRSINRAEUniversity Paris–Saclay91405 OrsayFrance

Fertilization constitutes a critical step in the plant life cycle during which the gamete genomes undergo chromatin dynamics in preparation for embryogenesis. In mammals, parental chromatin is extensively reprogrammed through the global erasure of DNA methylation. However, in flowering plants it remains unclear whether and how DNA methylation is remodeled in gametes and after fertilization in the zygote. In this study, we characterize DNA methylation patterns and investigate the function of DNA glycosylases in rice eggs, sperm, and unicellular zygotes and during embryogenesis. We found that DNA methylation is locally reconfigured after fertilization and is intensified during embryogenesis. Genetic, epigenomic, and transcriptomic analysis revealed that three rice DNA glycosylases, DNG702, DNG701, and DNG704, demethylate DNA at distinct genomic regions in the gametes and the zygote, and are required for zygotic gene expression and development. Collectively, these results indicate that active DNA demethylation takes place in the gametes and the zygote to locally remodel DNA methylation, which is critical for egg and zygote gene expression and reproduction in rice.

关键词： DNA methylation DNA glycosylase fertilization

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Prophylactic effects of Edible Lilium-Egg yolk compound powder on depression-like behaviors in rats exposed to chronic unpredictable mild stress: A role of gut-brain axis

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Food Science and Human Wellness 2024年

作者： Yu Huang Yu-xin Luo Sai-nan Tian Meng-zhou Xie Liang Li Ming-xing jiang Zhi-ying Yuan Hunan University of Chinese Medicine Key Laboratory of TCM heart and Lung Syndrome Differentiation & Medicated Diet and Dietotherapy， Hunan University of Chinese Medicine TCM and Ethnomedicine Innovation & Development International Laboratory， School of Pharmacy， Hunan University of Chinese Medicine

The incidence rate and harmfulness of depression are increasing yearly， and the research on the antidepressant effect of regulating the intestinal microecology balance has attracted widespread attention. The purpose of this research was to study the preventive effects and the regulatory mechanism of edible Lilium-egg yolk compound powder(Chinese abbreviation， BHT) on gut-brain crosstalk in depressive rats induced by chronic unpredictable mild stress(CUMS) through the "microbiota-gut-brain" axis. Results showed that infusion of BHT for 21 days ameliorated the morphology of the hippocampus and colon， increased the rate of sucrose preference， reduced the immobility time of forced swimming， increased the expression level of anti-inflammatory cytokine Interleukin-10 (IL-10) in serum， up-regulate the expression level of plasma neurotransmitter 5-hydroxy tryptamine (5-HT)， down-regulate the expression of inflammatory factors tumor necrosis factor-α (TNF-α)， interleukin-6 (IL-6)， and interleukin-1β (IL-1β) and neurotransmitter glutamic acid (Glu) in serum， and decrease the expression levels of lipopolysaccharide (LPS)， diamine oxidase (dao) and lactate dehydrogenase (LD) in plasma. The underlying mechanism may be to reduce the activation of microglia (lower levels of CD11b， IBa1 protein and gene expression)， regulate hippocampal GR/BDNF/NPY signal pathway (higher levels of GR， BDNF， NPY protein and gene expression)， enhance intestinal mucosal barrier function (higher levels of ZO-1， Occludin protein and gene expression)， regulate the composition and structure of intestinal flora(Analysis results of LEfSe combined with random forest showed that Prevotella and Paraprevotella might be biomarkers of BHT intervention in depression)， and it is related to the improvement of glutathione metabolism， and other metabolic pathways. Pearson correlation analysis found that 5-HT is strongly positively correlated with Prevotella，and Glu has a strong negative correlation with ***

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“两尘四气”空气污染数据的校准模型

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环境科学导刊 2024年第1期43卷 42-47,91页

作者：朱莹姜道旭盛俊李建龙扬州职业大学江苏扬州225009

通过Excel软件对监测点收集的空气质量数据进行拆分及构造函数的方法,拟合了“两尘四气”的六个方程,这些方程可用于对自建点数据进行校准。在对所收集的数据标准化后,利用SPSS软件对“两尘四气”数据误差产生的原因进行回归分析,建立... 详细信息

通过Excel软件对监测点收集的空气质量数据进行拆分及构造函数的方法,拟合了“两尘四气”的六个方程,这些方程可用于对自建点数据进行校准。在对所收集的数据标准化后,利用SPSS软件对“两尘四气”数据误差产生的原因进行回归分析,建立了多元线性回归方程。方程显示:压强、温度和风速更容易导致自建点数据误差,可使用对上述三种因素不敏感的材质制造检测仪的传感器。以上使用Excel和SPSS软件对大数据进行比对处理及建模的方法可用于环境监测、农业生产等领域促进高质量生态文明建设。

关键词： “两尘四气” Excel和SPSS 曲线拟合多元线性回归

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先秦文学神游书写的文化隐义

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中州学刊 2024年第8期 152-160页

作者：蒋振华湖南师范大学文学院湖南长沙410081

先秦文学中有丰富的神游书写,其所涉及山水客体内隐着丰富的文化要义。时间流逝伴随着神游主体记忆的消亡,时间永恒引发的理想诉求,有无相生,动机效果关系,凡此种种,体现了古人对于山水的哲学思辨。神游主体之于山水客体的美丑感受,神... 详细信息

先秦文学中有丰富的神游书写,其所涉及山水客体内隐着丰富的文化要义。时间流逝伴随着神游主体记忆的消亡,时间永恒引发的理想诉求,有无相生,动机效果关系,凡此种种,体现了古人对于山水的哲学思辨。神游主体之于山水客体的美丑感受,神游书写展示出的雄伟、缥缈、轻盈、朦胧、谲怪、奇诡等审美意象,开发了中国古典美学多种审美范式。神游中的山水与神仙的结盟,固化为一种“仙山”理念,开创了中国本土宗教中仙道文化的先河。这些文化隐义对中国古代文学中的神仙赋、游仙诗、玄言诗、梦境文学的形成产生了深远的影响。

关键词：先秦文学神游书写哲思美丑仙道

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Ten-channel mode-division-multiplexed silicon photonic integrated circuit with sharp bends

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Frontiers of Information Technology & Electronic Engineering 2019年第4期20卷 498-506页

作者： Chen-lei LI Xiao-hui jiang Yung HSU Guan-hong CheN Chi-wai CHOW dao-xin DAI Centre for Optical and Electromagnetic Research State Key Laboratory for Modern Optical InstrumentationCollege of Optical Science and Engineering Zhejiang University Ningbo Research Institute Zhejiang University Department of Photonics and Institute of Electro-Optical Engineering Chiao Tung University

A multimode silicon photonic integrated circuit(PIC) comprising a pair of on-chip mode(de)multiplexers with 10-mode channels and a multimode bus waveguide with sharp bends is demonstrated to enable multi-channel on-chip transmissions. The core width of the multimode bus waveguide is chosen such that it can support 10 guided modes, of which there are four transverse-magnetic polarization modes and six transverse-electric polarization modes. This multimode bus waveguide comprises sharp bends based on modified Euler curves. Experimental results demonstrate that the present silicon PIC enables the 10-channel on-chip transmission with a low inter-mode crosstalk of approximately-20 dB over a broad bandwidth of 1520–1610 nm even when the bending radius of the S-bend is as small as 40 μm. Compared with a silicon PIC using a conventional arc-bend with the same bending radius, our proposed PIC demonstrates a significant improvement.

关键词： Silicon Multimode Waveguide Euler-bends

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